Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,359,053	A→G	G45G (GGT→GGC)	puuP ←	putrescine:H(+) symporter PuuP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,359,053	0	A	G	100.0%	132.8 / NA	38	G45G (GGT→GGC)	puuP	putrescine:H(+) symporter PuuP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/18);  total (20/18)

CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATCACCACCTGCCACAATTTCAGTGATTTTCGCAGACGGGTTTTGCCGGGTTGCGCAGCAATATTCAGTGGTGAATTAATAGCC  >  NC_000913/1358918‑1359185                                                                                                                                        |                                                                                                                                     cgcgTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCaa                                                                                                                                  >  2:273650/1‑140 (MQ=255) cgcgTAGCTATAGGCCGAACCGGCCCCCGGAAACAGGCGAACCAGTTTGCTGTAGCTGATGGCGGTAAACAGCGCACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCaa                                                                                                                                  <  2:612236/140‑1 (MQ=255)   cgTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCCCACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCaaaa                                                                                                                                <  2:140101/140‑1 (MQ=255)      aGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTa                                                                                                                             <  1:494473/140‑1 (MQ=255)       ggTATAGGCCGACCCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGTCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTAt                                                                                                                            <  2:656492/140‑1 (MQ=255)                  aaCCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTc                                                                                                                 >  2:909000/1‑140 (MQ=255)                  aaCCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTc                                                                                                                 >  2:208019/1‑140 (MQ=255)                  aaCCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTc                                                                                                                 >  1:334500/1‑140 (MQ=255)                  aaCCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTc                                                                                                                 >  2:30106/1‑140 (MQ=255)                  aaCCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAAAGCCGGAGACAATGCCAAAAGTATCAAATACGGTc                                                                                                                 >  1:922583/1‑140 (MQ=255)                  aaCCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCCCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTc                                                                                                                 >  2:841975/1‑140 (MQ=255)                    ccGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCAt                                                                                                               <  1:30106/140‑1 (MQ=255)                       gCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCgg                                                                                                            >  1:513624/1‑140 (MQ=255)                       gCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCgg                                                                                                            >  1:383261/1‑140 (MQ=255)                         cTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCg                                                                                                          <  1:289199/140‑1 (MQ=255)                         cTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCg                                                                                                          <  1:208019/140‑1 (MQ=255)                              gAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCGTgaga                                                                                                     <  1:909000/140‑1 (MQ=255)                                  cTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCGTGAGATAgg                                                                                                 <  2:513624/140‑1 (MQ=255)                                  cTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCGTGAGATAgg                                                                                                 <  2:383261/140‑1 (MQ=255)                                     gCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCAt                                                                                                               <  1:206988/123‑1 (MQ=255)                                     gCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCAt                                                                                                               >  2:206988/1‑123 (MQ=255)                                      cGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAg                                                                                             <  1:273650/140‑1 (MQ=255)                                                               cGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATCACCACCTGCCACAAt                                                                    >  1:860645/1‑140 (MQ=255)                                                                         cacaCCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATCACCACCTGCCACAATTTCAGTGAtt                                                          >  2:944337/1‑140 (MQ=255)                                                                         cacaCCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATCACCACCTGCCACAATTTCAGTGAtt                                                          >  1:698653/1‑140 (MQ=255)                                                                              ccGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGCATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATCACCACCTGCCACAATTTCAGTGATTTTCGc                                                     >  1:456936/1‑140 (MQ=255)                                                                                gccagcgccagcAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATCACCACCTGCCACAATTTCAGTGATTTTCGCAg                                                   >  2:428098/1‑140 (MQ=255)                                                                                     cgccagcAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATCACCACCTGCCACAATTTCAGTGATTTTCGCAGACggg                                              <  1:944337/140‑1 (MQ=255)                                                                                                  gACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATCACCACCTGCCACAATTTCAGTGATTTTCGCAGACGGGTTTTGCCGGGTTg                                 >  1:100262/1‑140 (MQ=255)                                                                                                   aCGCCGGAACGTGATCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATCACCACCTGCCACAATTTCAGTGATTTTCGCAGACGGGTTTTGCCGGGTTgc                                >  2:16919/1‑140 (MQ=255)                                                                                                    cGCCGGAACGTGATCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATCACCACCTGCCACAATTTCAGTGATTTTCGCAGACGGGTTTTGCCGGGTTgcg                               <  1:16919/140‑1 (MQ=255)                                                                                                    cGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATCACCACCTGCCACAATTTCAGTGATTTTCGCAGACGGGTTTTGCCGGGTTgcg                               <  1:428098/140‑1 (MQ=255)                                                                                                       cGGAACGTGCCCGTCGCAAATCCGGGAAACAATGCCAAAAGTATCAAATACGGTCATCGCGGTGAAATAGGCCGGACCCATCTTCACCACCTGCCACAATTTCAGTGATTTTCGCAGACGGGTTTTGCCGGGTTGCgcag                            <  2:456936/140‑1 (MQ=255)                                                                                                       cGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATCACCACCTGCCACAATTTCAGTGATTTTCGCAGACGGGTTTTg                                         >  2:514775/1‑127 (MQ=255)                                                                                                       cGGAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATCACCACCTGCCACAATTTCAGTGATTTTCGCAGACGGGTTTTg                                         <  1:514775/127‑1 (MQ=255)                                                                                                         gAACGTGACCGTCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATCACCACCTGCCACAATTTCAGTGATTTTCGCAGACGGGTTTTGCCGGGTTGCgcagca                          <  1:841975/140‑1 (MQ=255)                                                                                                              tGACCGGCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATCACCACCTGCCACAATTTCAGTGATTTTCGCAGACGGGTTTTGCCGGGTTGCGCAGCAATAtt                     <  2:334500/140‑1 (MQ=255)                                                                                                                    tCGCTAATGCCGGAGACAATGCCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATCACCACCTGCCACAATTTCAGTGATTTTCGCAGACGGGTTTTGCCGGGTTGCGCAGCAATATTCAgtgg               >  1:545855/1‑140 (MQ=255)                                                                                                                                 agaCAATGCCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATCACCACCTGCCACAATTTCAGTGATTTTCGCAGACGGGTTTTGCCGGGTTGCGCAGCAATATTCAGTGGTGAATTAATAGcc  >  2:514943/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                     CGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATCACCACCTGCCACAATTTCAGTGATTTTCGCAGACGGGTTTTGCCGGGTTGCGCAGCAATATTCAGTGGTGAATTAATAGCC  >  NC_000913/1358918‑1359185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑