Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,432,087	C→T	G1013G (GGC→GGT)	stfR →	Rac prophage; putative membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,432,087	0	C	T	100.0%	266.0 / NA	75	G1013G (GGC→GGT)	stfR	Rac prophage; putative membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (42/33);  total (42/33)

AGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCATCCAGTACAGATTTGGGGACGAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGCTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACTCCGGTGCTGGTAGTGCATCAACAAGATTGTCTGTTGTGCATAATCAAAACTATGCAACATCATCTGCTGG  >  NC_000913/1431954‑1432221                                                                                                                                      |                                                                                                                                       aGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCATCCAGTGCAGATTTGGGGACGAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACa                                                                                                                                  >  1:150555/1‑140 (MQ=255) aGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCATCCAGTACAGATTTGGGGACGAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACa                                                                                                                                  >  2:851018/1‑140 (MQ=255) aGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCATCCAGTACAGATTTGGGGACGAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACa                                                                                                                                  >  2:72876/1‑140 (MQ=255) aGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCATCCAGTACAGATTTGGGGACGAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACa                                                                                                                                  >  2:312436/1‑140 (MQ=255) aGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCATCCAGTACAGATTTGGGGACGAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACa                                                                                                                                  >  2:530749/1‑140 (MQ=255)   gACGGCATTAAATCGCATACCCACAGCGCCAGCGCATCCAGTACAGATTTGGGGACGAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACaaa                                                                                                                                >  2:865721/1‑140 (MQ=255)   gACGGCATTAAATCGCATACCCACAGCGCCAGCGCATCCAGTACAGATTTGGGGACGAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACaaa                                                                                                                                >  2:217265/1‑140 (MQ=255)     cGGCATTAAATCGCATACCCACAGCGCCAGCGCATCCAGTACAGATTTGGGGACGAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACt                                                                                                                              >  2:756116/1‑140 (MQ=255)           tAAATCGCATACCCACAGCGCCAGCGCATCCAGTACAGATTTGGGGACGAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTg                                                                                                                        <  2:820310/140‑1 (MQ=255)                cGCATACCCACAGCGCCAGCGCATCCAGTACAGATTTGGGGACGAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGca                                                                                                                   <  1:530749/140‑1 (MQ=255)                  cATACCCACAGCGCCAGCGCATCCAGTAGAGATTTGGGGACGAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGcaca                                                                                                                 >  1:712843/1‑140 (MQ=255)                  cATACCCACAGCGCCAGCGCATCCAGTACAGATTTGGGGACGAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCt                                                                                                                         <  1:298057/132‑1 (MQ=255)                  cATACCCACAGCGCCAGCGCATCCAGTACAGATTTGGGGACGAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCt                                                                                                                         >  2:298057/1‑132 (MQ=255)                   aTACCCACAGCGCCAGCGCATCCAGTACAGATTTGGGGACGAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGcacac                                                                                                                <  2:699491/140‑1 (MQ=255)                   aTACCCACAGCGCCAGCGCATCCAGTACAGATTTGGGGACGAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGcacac                                                                                                                <  1:60836/140‑1 (MQ=255)                       ccACAGCGCCAGCGCATCCAGTACAGATTTGGGGACGAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGcacacacac                                                                                                            <  1:865721/140‑1 (MQ=255)                       ccACAGCGCCAGCGCATCCAGTACAGATTTGGGGACGAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGcacacacac                                                                                                            >  2:202885/1‑140 (MQ=255)                          cagcgccagcgcATCCAGTACAGATTTGGGGACGAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACAcact                                                                                                         >  1:467217/1‑140 (MQ=255)                           agcgccagcgcATCCAGTACAGATTTGGGGACGAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACAcactc                                                                                                        >  1:76147/1‑140 (MQ=255)                            gcgccagcgcATCCAGTACAGATTTGGGGACGAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACAcactca                                                                                                       <  1:312436/140‑1 (MQ=255)                             cgccagcgcATCCAGTACAGATTTGGGGACGAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGcacacacac                                                                                                            <  1:367995/134‑1 (MQ=255)                             cgccagcgcATCCAGTACAGATTTGGGGACGAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGcacacacac                                                                                                            >  2:367995/1‑134 (MQ=255)                                cagcgcATCCAGTACAGATTTGGGGACGAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAg                                                                                                   >  2:452355/1‑140 (MQ=255)                                 agcgcATCCAGTACAGATTTGGGGACGAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGc                                                                                                  <  1:851018/140‑1 (MQ=255)                                   cgcATCCAGTACAGATTTGGGGACGAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGcaca                                                                                                                 <  1:335213/123‑1 (MQ=255)                                   cgcATCCAGTACAGATTTGGGGACGAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGcaca                                                                                                                 >  2:335213/1‑123 (MQ=255)                                      aTCCAGTACAGATTTGGGGACGAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACg                                                                                             <  1:202885/140‑1 (MQ=255)                                          aGTACAGATTTGGGGACGAAAACCACGTCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGaa                                                                                         <  1:39353/140‑1 (MQ=255)                                                 aTTTGGGGACGAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCt                                                                                  >  2:915666/1‑140 (MQ=255)                                                    tGGGGACGAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGt                                                                               <  2:712843/140‑1 (MQ=255)                                                    tGGGGACGAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGt                                                                               >  1:590094/1‑140 (MQ=255)                                                      gggACGAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGc                                                                                   >  2:49754/1‑134 (MQ=255)                                                      gggACGAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGc                                                                                   <  1:49754/134‑1 (MQ=255)                                                      gggACGAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTa                                                                                 >  1:344877/1‑136 (MQ=255)                                                      gggACGAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTa                                                                                 <  2:344877/136‑1 (MQ=255)                                                      gggACGAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGc                                                                             >  1:184549/1‑140 (MQ=255)                                                           gAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCGACGTGAACACGGCTAGTGCTAACt                                                                        <  1:72876/140‑1 (MQ=255)                                                           gAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACt                                                                        <  1:756116/140‑1 (MQ=255)                                                             aaaCCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACTcc                                                                      <  1:217265/140‑1 (MQ=255)                                                              aaCCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACTCCg                                                                     <  1:930664/140‑1 (MQ=255)                                                                  acaTCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGTGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACTCCGGTGc                                                                 <  1:452355/140‑1 (MQ=255)                                                                        tcgtTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTACTAACTCCGGTGCTGGTAg                                                           <  1:652548/140‑1 (MQ=255)                                                                               aTTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACTCCGGTGCTGGTAGTGCATca                                                    >  2:710454/1‑140 (MQ=255)                                                                                    ggCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACTCCGGTGCTGGTAGTGCATCAACAAg                                               <  1:709756/140‑1 (MQ=255)                                                                                      cACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACTCCGGTGCTGGTAGTGCATCAACAAg                                               >  1:181472/1‑138 (MQ=255)                                                                                      cACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACTCCGGTGCTGGTAGTGCATCAACAAg                                               <  2:181472/138‑1 (MQ=255)                                                                                         tAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACTCCGGTGCTGGTAGTGCATcaaca                                                 <  1:814182/133‑1 (MQ=255)                                                                                         tAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACTCCGGTGCTGGTAGTGCATcaaca                                                 >  2:814182/1‑133 (MQ=255)                                                                                         tAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACTCCGGTGCTGGTAGTGCATCAACAAGATTGt                                          <  2:184549/140‑1 (MQ=255)                                                                                          aaaTCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACTCCGGTGCTGGTAGTGCATCAACAAGATTGTc                                         >  1:237670/1‑140 (MQ=255)                                                                                           aaTCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACTCCGGTGCTGGTAGTGCATCAACAAGATTGTCt                                        >  2:522445/1‑140 (MQ=255)                                                                                            aTCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACTCCGGTGCTGGTAGTGCATCAACAAGATTGTCtg                                       <  1:767724/140‑1 (MQ=255)                                                                                              ccACGAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACTCCGGTGCTGGTAGTGCATCAACAAGATTGTCtgtt                                     <  2:237670/140‑1 (MQ=255)                                                                                                  gAATAACACCGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACTCCGGTGCTGGTAGTGCATCAACAAGATTGTCTGTTGTGc                                 <  2:320063/140‑1 (MQ=255)                                                                                                           cGGGGCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACTCCGGTGCTGGTAGTGCATCAACAAGATTGTCTGTTGTGCATAATCaaa                        <  2:76147/140‑1 (MQ=255)                                                                                                            ggggCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACTCCGGTGCTGGTAGTGCATCAACAAGATTGTCTGTTGTGCATAATCaaaa                       <  1:710454/140‑1 (MQ=255)                                                                                                            ggggCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACTCCGGTGCTGGTAGTGCATCAACAAGATTGTCTGTTGTGCATAATCaaaa                       >  2:561396/1‑140 (MQ=255)                                                                                                             gggCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACTCCGGTGCTGGTAGTGCATCAACAAGATTGTCTGTTGTGCATAATCAAAAc                      >  1:591218/1‑140 (MQ=255)                                                                                                             gggCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACTCCGGTGCTGGTAGTGCATCAACAAGATTGTCTGTTGTGCATAATCAAAAc                      >  1:622889/1‑140 (MQ=255)                                                                                                              ggCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACTCCGGTGCTGGTAGTGCATCAACAAGATTGTCTGTTGTGc                                 >  2:608573/1‑128 (MQ=255)                                                                                                              ggCACATACACACAGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACTCCGGTGCTGGTAGTGCATCAACAAGATTGTCTGTTGTGc                                 <  1:608573/128‑1 (MQ=255)                                                                                                                        cacaGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACTCCGGTGCTGGTAGTGCATCAACAAGATTGTCTGTTGTGCATAATCAAAACTATGCAAcatc           >  1:127461/1‑140 (MQ=255)                                                                                                                         acaGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACTCCGGTGCTGGTAGTGCATCAACAAGATTGTCTGTTGTGCATAATCAAAACTATGCAAcatca          >  1:600730/1‑140 (MQ=255)                                                                                                                           aGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACTCCGGTGCTGGTAGTGCATCAACAAGATTGTCTGTTGTGCATAATCaaaa                       >  1:357775/1‑125 (MQ=255)                                                                                                                           aGTGTGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACTCCGGTGCTGGTAGTGCATCAACAAGATTGTCTGTTGTGCATAATCaaaa                       <  2:357775/125‑1 (MQ=255)                                                                                                                            gtgtGAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACTCCGGTGCTGGTAGTGCATCAACAAGATTGTCTGTTGTGCATAATCAAAACTATGCAACATCATct       >  2:300340/1‑140 (MQ=255)                                                                                                                             tgtgAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACTCCGGTGCTGGTAGTGCATCAACAAGATTGTCTGTTGTGCATAATCAAAACTATGCAACATCATctg      >  1:81663/1‑140 (MQ=255)                                                                                                                             tgtgAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACTCCGGTGCTGGTAGTGCATCAACAAGATTGTCTGTTGTGCATAATCAAAACTATGCAACATCATctg      >  2:605868/1‑140 (MQ=255)                                                                                                                             tgtgAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACTCCGGTGCTGGTAGTGCATCAACAAGATTGTCTGTTGTGCATAATCAAAACTATGCAACATCATctg      >  2:528098/1‑140 (MQ=255)                                                                                                                             tgtgAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACTCCGGTGCTGGTAGTGCATCAACAAGATTGTCTGTTGTGCATAATCAAAACTATGCAACATCATctg      >  2:81701/1‑140 (MQ=255)                                                                                                                               tgAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACTCCGGTGCTGGTAGTGCATCAACAAGATTGTCTGTTGTGCATAATCAAAACTATGCAACATCATctgct    >  2:777546/1‑140 (MQ=255)                                                                                                                               tgAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACTCCGGTGCTGGTAGTGCATCAACAAGATTGTCTGTTGTGCATAATCAAAACTATGCAACATCATctgct    >  2:813894/1‑140 (MQ=255)                                                                                                                               tgAGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACTCCGGTGCTGGTAGTGCATCAACAAGATTGCCTGTTGTGCATAATCAAAACTATGCAACATCATctgct    >  2:148480/1‑140 (MQ=255)                                                                                                                                 aGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACTCCGGTGCTGGTAGTGCATCAACAAGATTGTCTGTTGTGCATAATCAAAACTATGCAACATCATCTGCTgg  <  2:622889/140‑1 (MQ=255)                                                                                                                                 aGCGGTTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACTCCGGTGCTGGTAGTGCATCAACAAGATTGTCTGTTGTGCATAATCAAAACTATGCAACATCATCTGCTgg  >  1:289190/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                       AGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCATCCAGTACAGATTTGGGGACGAAAACCACATCGTCGTTTGATTACGGCACTAAATCCACGAATAACACCGGGGCACATACACACAGTGTGAGCGGCTCTACAAACTCGGCTGGAGCACACACACACTCACTAGCCAACGTGAACACGGCTAGTGCTAACTCCGGTGCTGGTAGTGCATCAACAAGATTGTCTGTTGTGCATAATCAAAACTATGCAACATCATCTGCTGG  >  NC_000913/1431954‑1432221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑