Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,541,351	C→T	E414K (GAA→AAA)	narZ ←	nitrate reductase Z subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,541,351	0	C	T	100.0%	184.0 / NA	52	E414K (GAA→AAA)	narZ	nitrate reductase Z subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/24);  total (28/24)

GTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTCGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCGGATTGTTGCT  >  NC_000913/1541214‑1541486                                                                                                                                          |                                                                                                                                        gTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTgg                                                                                                                                       >  1:663730/1‑140 (MQ=255)           ggCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTAcc                                                                                                                             >  2:413343/1‑140 (MQ=255)             cAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACcgg                                                                                                                           <  1:769154/140‑1 (MQ=255)                            cACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGgattcca                                                                                                            <  2:797232/140‑1 (MQ=255)                                 gATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGgattccagattc                                                                                                       >  2:250840/1‑140 (MQ=255)                                 gATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGgattccagattc                                                                                                       >  1:65843/1‑140 (MQ=255)                                      tgtTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCAttt                                                                                                  <  2:65843/140‑1 (MQ=255)                                           tACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCtt                                                                                             >  2:800051/1‑140 (MQ=255)                                           tACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCtt                                                                                             >  2:274140/1‑140 (MQ=255)                                                tGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTg                                                                                                 >  1:131155/1‑131 (MQ=255)                                                tGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTg                                                                                                 <  2:131155/131‑1 (MQ=255)                                                tGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTtct                                                                                        >  2:279078/1‑140 (MQ=255)                                                tGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGCTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTtct                                                                                        >  2:860531/1‑140 (MQ=255)                                                        tGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCg                                                                                <  2:360999/140‑1 (MQ=255)                                                                 tCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAAt                                                                       >  1:3139/1‑140 (MQ=255)                                                                     tGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGaa                                                                   <  2:3139/140‑1 (MQ=255)                                                                       ccgccAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAAcc                                                                 >  2:900743/1‑140 (MQ=255)                                                                             aaGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCgg                                                           <  1:860531/140‑1 (MQ=255)                                                                                 aGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACt                                                       <  2:663730/140‑1 (MQ=255)                                                                                    ggAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTAcc                                                    >  1:130479/1‑140 (MQ=255)                                                                                        ggCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAAtt                                                >  2:291873/1‑140 (MQ=255)                                                                                          ccACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCa                                              >  2:707010/1‑140 (MQ=255)                                                                                          ccACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCa                                              >  2:520357/1‑140 (MQ=255)                                                                                           cACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCAc                                             <  1:800051/140‑1 (MQ=255)                                                                                           cACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCAc                                             <  1:945334/140‑1 (MQ=255)                                                                                           cACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATCCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCGCTACCAATTCAc                                             <  2:342552/140‑1 (MQ=255)                                                                                             cGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGTAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCt                                           <  2:547804/140‑1 (MQ=255)                                                                                             cGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCt                                           <  2:741365/140‑1 (MQ=255)                                                                                             cGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCt                                           >  2:643612/1‑140 (MQ=255)                                                                                              gCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTg                                          <  1:437454/140‑1 (MQ=255)                                                                                               ccTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGc                                         <  1:413343/140‑1 (MQ=255)                                                                                               ccTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGc                                         <  1:805589/140‑1 (MQ=255)                                                                                                cTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCg                                        <  1:274140/140‑1 (MQ=255)                                                                                                   cAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGGAACCAATCGAACCGTTCGGCCCTACCAATTc                                               >  1:81636/1‑130 (MQ=255)                                                                                                   cAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTc                                               <  2:81636/130‑1 (MQ=255)                                                                                                         cGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACt                               >  1:380403/1‑140 (MQ=255)                                                                                                         cGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGAGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGc                                         >  1:729281/1‑130 (MQ=255)                                                                                                         cGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGAGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGc                                         <  2:729281/130‑1 (MQ=255)                                                                                                             aTGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTa                           >  2:56200/1‑140 (MQ=255)                                                                                                             aTGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTa                           <  1:520357/140‑1 (MQ=255)                                                                                                              tGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCt                                           <  1:524376/123‑1 (MQ=255)                                                                                                              tGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCt                                           >  2:524376/1‑123 (MQ=255)                                                                                                              tGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTAc                          >  2:721211/1‑140 (MQ=255)                                                                                                                   aCCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTaa                                 <  2:558262/128‑1 (MQ=255)                                                                                                                   aCCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTaa                                 >  1:558262/1‑128 (MQ=255)                                                                                                                   aCCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGttt                     >  2:199003/1‑140 (MQ=255)                                                                                                                   aCCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCC‑TTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGtttt                    >  1:510254/1‑140 (MQ=255)                                                                                                                          agtgTTAACGACAACTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTg              <  2:583376/137‑1 (MQ=255)                                                                                                                             gTTAACTACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCgg           >  1:386475/1‑140 (MQ=255)                                                                                                                             gTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCgg           >  1:768500/1‑140 (MQ=255)                                                                                                                                 aCGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCGGAttg       <  2:380403/140‑1 (MQ=255)                                                                                                                                      aaTTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCGGATTGTTGCt  >  1:593238/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        GTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTCGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCGGATTGTTGCT  >  NC_000913/1541214‑1541486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑