Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	191,440	A→G	Y195C (TAC→TGC)	tsf →	protein chain elongation factor EF‑Ts

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	191,440	0	A	G	100.0%	140.3 / NA	41	Y195C (TAC→TGC)	tsf	protein chain elongation factor EF‑Ts
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/18);  total (23/18)

TGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCGCTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAAGCAA  >  NC_000913/191303‑191575                                                                                                                                          |                                                                                                                                        tGCGCGTATCGGCGTTCTGGTTGCTGCTAACGGCGCTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATGcc                                                                                                                                       >  2:691415/1‑140 (MQ=255) tGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCGCTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATGcc                                                                                                                                       >  2:271885/1‑140 (MQ=255) tGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCGCTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATGcc                                                                                                                                       >  1:259292/1‑140 (MQ=255) tGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCGCTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATGcc                                                                                                                                       >  2:786967/1‑140 (MQ=255) tGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCGCTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATGcc                                                                                                                                       >  1:460662/1‑140 (MQ=255) tGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCGCTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATGcc                                                                                                                                       >  2:924187/1‑140 (MQ=255)           ggCGTTCTGGTTGCTGCTAAAGGCGCTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGTAGTGGTAGAAAAAGAATGCCAGGTACAGCt                                                                                                                             >  2:581907/1‑140 (MQ=255)             cGTTCTGGTTGCTGCTAAAGGCGCTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATGCCAGGTACAGCTgg                                                                                                                           <  1:271885/140‑1 (MQ=255)              gTTCTGGTTGCTGCTAAAGGCGCTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATGCCAGGTACAGCTGGa                                                                                                                          <  1:854832/140‑1 (MQ=255)              gTTCTGGTTGCTGCTAAAGGCGCTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATGCCAGGTACAGCTGGa                                                                                                                          <  1:302847/140‑1 (MQ=255)                tCTGGTTGCTGCTAAAGGCGCTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATGCCAGGTACAGCTGGata                                                                                                                        >  2:164939/1‑140 (MQ=255)                     ttGCTGCTAAAGGCGCTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGACAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATGCCAGGTACAGCTGGATATcgcg                                                                                                                   <  1:748666/140‑1 (MQ=255)                       gctgctAAAGG‑GCTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATGCCAGGTACAGCTGGATATCGCGATg                                                                                                                <  1:728319/140‑1 (MQ=255)                         tgctAAAGGCGCTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATGCCAGGTACAGCTGGATATCGCGATGc                                                                                                               <  1:738993/140‑1 (MQ=255)                          gctAAAGGCGCTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATGCCAGGTACAGCTGGATATCGCGATGCa                                                                                                              >  1:693226/1‑140 (MQ=255)                                  cgcTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATGCCAGGTACAGCTGGATATCGCGATGCAGTCTGGTa                                                                                                      >  1:15536/1‑140 (MQ=255)                                     tGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATGCCAGGTACAGCTGGATATCGCGATGCAGTCTGGt                                                                                                       >  2:877891/1‑136 (MQ=255)                                     tGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATGCCAGGTACAGCTGGATATCGCGATGCAGTCTGGt                                                                                                       <  1:877891/136‑1 (MQ=255)                                                 ggTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATGCCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCg                                                                                       >  1:28123/1‑140 (MQ=255)                                                   ttAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATGCCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCa                                                                                     <  2:157957/140‑1 (MQ=255)                                                   ttAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATGCCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCa                                                                                     <  2:15536/140‑1 (MQ=255)                                                       acacATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATGCCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAgaga                                                                                 <  2:846348/140‑1 (MQ=255)                                                          caTCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATGCCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGaaaa                                                                              <  1:164939/140‑1 (MQ=255)                                                                       gTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATGCCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCg                                                                 >  1:222003/1‑140 (MQ=255)                                                                         tgctgcAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATGCCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCa                                                               >  1:239734/1‑140 (MQ=255)                                                                            tgcAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATGCCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATga                                                            >  1:577044/1‑140 (MQ=255)                                                                              caagcaagCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATGCCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATgaag                                                          >  1:300506/1‑140 (MQ=255)                                                                                   aagcCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATGCCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAAtt                                                     >  1:924676/1‑140 (MQ=255)                                                                                   aagcCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATGCCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAAtt                                                     >  1:818134/1‑140 (MQ=255)                                                                                     gcCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATGCCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCa                                                   >  2:946057/1‑140 (MQ=255)                                                                                                       ggAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATGCCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGa                                 <  1:691415/140‑1 (MQ=255)                                                                                                               tATCCGCTGAAGTGGTAGAAAAAGAATGCCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAg                         >  1:412770/1‑140 (MQ=255)                                                                                                                 tCCGCTGAAGTGGTAGAAAAAGAATGCCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGcc                       <  1:946057/140‑1 (MQ=255)                                                                                                                    gCTGAAGTGGTAGAAAAAGAATGCCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCcgtt                    <  2:300506/140‑1 (MQ=255)                                                                                                                    gCTGAAGTGGTAGAAAAAGAATGCCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCcgtt                    <  2:577044/140‑1 (MQ=255)                                                                                                                    gCTGAAGTGGTAGAAAAAGAATGCCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCcgtt                    <  2:693226/140‑1 (MQ=255)                                                                                                                    gCTGAAGTGGTAGAAAAAGAATGCCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCcgtt                    <  1:581907/140‑1 (MQ=255)                                                                                                                      tGAAGTGGTAGAAAAAGAATGCCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCcgttcg                  >  2:136387/1‑140 (MQ=255)                                                                                                                      tGAAGTGGGAGAAAAAGAATGCCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCcgttcg                  >  2:451475/1‑140 (MQ=255)                                                                                                                        aaGTGGTAGAAAAAGAATGCCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCcgttcgtt                >  1:782152/1‑140 (MQ=255)                                                                                                                                gAAAAAGAATGCCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAAcc        >  1:156075/1‑140 (MQ=255)                                                                                                                                      gAATGCCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAAGCaa  <  1:441193/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        TGCGCGTATCGGCGTTCTGGTTGCTGCTAAAGGCGCTGACGAAGAGCTGGTTAAACACATCGCTATGCACGTTGCTGCAAGCAAGCCAGAATTCATCAAACCGGAAGACGTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAAGCAA  >  NC_000913/191303‑191575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑