Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,612,237	C→T	C30Y (TGC→TAC)	yneG ←	conserved protein YneG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,612,237	0	C	T	100.0%	141.6 / NA	42	C30Y (TGC→TAC)	yneG	conserved protein YneG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/22);  total (20/22)

TGCTGAGCGATAAACACCGGATGCCCGCGCATGGGGGTTTGCTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGCAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAACGCGCGAAGAGTGGATCGAGAGACTGCATTAATAAACCGAACGCCCTAACTGTTTCGTCAATTGTTC  >  NC_000913/1612105‑1612363                                                                                                                                     |                                                                                                                               tgcagAGCGATAAACACCGGATGCCCGCGCATGGGGGTTTGCTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTg                                                                                                                         >  1:387294/5‑140 (MQ=255)    agAGCGATAAACACCGGATGCCCGCGCATGGGGGTTTGCTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACg                                                                                                                      <  2:387294/139‑1 (MQ=255)      aGCGATAAACACCGGATGCCCGCGCATGGGGGTTTGCTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGct                                                                                                                    >  1:360129/1‑140 (MQ=255)        cGATAAACACCGGATGCCCGCGCATGGGGGTTTGCTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGctct                                                                                                                  >  2:422955/1‑140 (MQ=255)        cGATAAACACCGGATGCCCGCGCATGGGGGTTTGCTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGctct                                                                                                                  >  2:454757/1‑140 (MQ=255)          aTAAACACCGGATGCCCGCGCATGGGGGTTTGCTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCttt                                                                                                                <  2:717603/140‑1 (MQ=255)           tAAACACCGGATGCCCGCGCATGGGGGTTTGCTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTc                                                                                                               >  1:41637/1‑140 (MQ=255)               cacCGGATGCCCGCGCATGGGGGTTTGCTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGc                                                                                                           <  2:41637/140‑1 (MQ=255)                acCGGATGCCCGCGCATGGGGGTTTGCTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGcc                                                                                                          >  1:560558/1‑140 (MQ=255)                 ccGGATGCCCGCGCATGGGGGTTTGCTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGccc                                                                                                         <  2:560558/140‑1 (MQ=255)                  cGGATGCCCGCGCATGGGGGTTTGCTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCa                                                                                                        >  1:494189/1‑140 (MQ=255)                         ccGCGCATGGGGGTTTGCTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGaaa                                                                                                 >  1:469711/1‑140 (MQ=255)                            cgcATGGGGGTTTGCTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCg                                                                                              >  2:865485/1‑140 (MQ=255)                                 gggggTTTGCTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGccc                                                                                                         <  2:686013/124‑1 (MQ=255)                                 gggggTTTGCTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGccc                                                                                                         >  1:686013/1‑124 (MQ=255)                                  ggggTTTGCTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCg                                                                                        <  1:790858/140‑1 (MQ=255)                                        tGCTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAAttt                                                                                  >  1:726783/1‑140 (MQ=255)                                          cTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTg                                                                                <  2:384872/140‑1 (MQ=255)                                            tACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGaa                                                                              >  2:845671/1‑140 (MQ=255)                                                   aTTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATa                                                                       >  1:439429/1‑140 (MQ=255)                                                     tAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATaaa                                                                     <  1:208535/140‑1 (MQ=255)                                                          ggTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAAcgcgc                                                                <  2:865089/140‑1 (MQ=255)                                                           gTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAAcgcgcg                                                               <  2:360129/140‑1 (MQ=255)                                                           gTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAAcgcgcg                                                               <  2:494189/140‑1 (MQ=255)                                                           gTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAAcgcgcg                                                               <  2:439429/140‑1 (MQ=255)                                                                gCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAACGCGCGAagag                                                          <  1:422955/140‑1 (MQ=255)                                                                  cGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATATTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAACGCGCGAAGAGTg                                                        <  2:517286/140‑1 (MQ=255)                                                                   ggAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAACGCGCGAag                                                            <  2:553677/135‑1 (MQ=255)                                                                   ggAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAACGCGCGAag                                                            >  1:553677/1‑135 (MQ=255)                                                                      gCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAACGCGCGAAGAGTGGATc                                                    >  1:879393/1‑140 (MQ=255)                                                                      gCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAACGCGCGAAGAGTGGATc                                                    >  1:160757/1‑140 (MQ=255)                                                                         aGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAACGCGCGAAGAGTGGATCgag                                                 >  1:936720/1‑140 (MQ=255)                                                                          gACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAACGCGCGAAGAGTGGATCgaga                                                <  1:845671/140‑1 (MQ=255)                                                                               ttAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAACGCGCGAAGAGTGGATCGAGAGACTg                                           <  2:726783/140‑1 (MQ=255)                                                                                 aGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAACGCGCGAAGAGTGGATCGAGAGACTGCa                                         >  1:91050/1‑140 (MQ=255)                                                                                   cGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAACGCGCGAAGAGTGGATCGAGAGACTGCAtt                                       >  1:818602/1‑140 (MQ=255)                                                                                              gccgcATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAACGCGCGAAGAGTGGATCGAGAGACTGCATTAATAAACCGaa                            <  2:879393/140‑1 (MQ=255)                                                                                              gccgcATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAACGCGCGAAGAGTGGATCGAGAGACTGCATTAATAAACCGaa                            <  2:160757/140‑1 (MQ=255)                                                                                                cgcATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAACGCGCGAAGAGTGGATCGAGAGACTGCATTAATAAACCGaa                            <  2:485221/138‑1 (MQ=255)                                                                                                cgcATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAACGCGCGAAGAGTGGATCGAGAGACTGCATTAATAAACCGaa                            >  1:485221/1‑138 (MQ=255)                                                                                                     gttgttCGATGACTGGCGCGCCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAACGCGCGAAGAGTGGATCGAGAGACTGCATTAATAAACCGAACGCCCTa                     <  1:483515/140‑1 (MQ=255)                                                                                                                        gcCTTTCTCCAGGTAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAACGCGCGAAGAGTGGATCGAGAGACTGCATTAATAAACCGAACGCCCTAACTGTTTCGTCAATTGTTc  <  2:936720/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                               TGCTGAGCGATAAACACCGGATGCCCGCGCATGGGGGTTTGCTTACCGTCATTAGCCGGTAATGCCGGAGCAAGACGTTTAGCGACAAAATCCGCCGCATGTTGTTCGATGACTGGCGCGCCTTTCTCCAGGCAATACTGACGCTCTTTCATGCCCAGACGAAAGCGAGAGCGAAATTTTGAACGGGATAAACGCGCGAAGAGTGGATCGAGAGACTGCATTAATAAACCGAACGCCCTAACTGTTTCGTCAATTGTTC  >  NC_000913/1612105‑1612363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑