Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,732,446	T→C	G1120G (GGT→GGC)	lhr →	putative ATP‑dependent helicase Lhr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,732,446	0	T	C	100.0%	190.6 / NA	53	G1120G (GGT→GGC)	lhr	putative ATP‑dependent helicase Lhr
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/29);  total (24/29)

GGTGAGCGATGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGCTTGCCGGAGTCGGTTTACCCGCCTCACTCTGGGAAAGCCAGATCCTGCCTGCCC  >  NC_000913/1732311‑1732576                                                                                                                                        |                                                                                                                                   ggTGAGCGATGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGcc                                                                                                                                >  2:710251/1‑140 (MQ=255)    gAGCGATGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCgg                                                                                                                             >  1:866474/1‑140 (MQ=255)     aGCGATGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGgc                                                                                                                            >  1:595773/1‑140 (MQ=255)      gCGATGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCC‑CCACCGATGGCAGCCCGgcgc                                                                                                                          >  1:112579/1‑140 (MQ=255)       cGATGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGgcgc                                                                                                                          >  1:681146/1‑140 (MQ=255)       cGATGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGgcgc                                                                                                                          >  1:658916/1‑140 (MQ=255)       cGATGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGgcgc                                                                                                                          >  1:755956/1‑140 (MQ=255)         aTGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGctc                                                                                                                        <  1:198321/140‑1 (MQ=255)         aTGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGctc                                                                                                                        <  1:121462/140‑1 (MQ=255)         aTGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGctc                                                                                                                        <  2:658916/140‑1 (MQ=255)         aTGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGctc                                                                                                                        <  2:681146/140‑1 (MQ=255)           gAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCtt                                                                                                                      <  1:165204/140‑1 (MQ=255)              gTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGc                                                                                                                   <  1:710251/140‑1 (MQ=255)               tATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGGTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGcc                                                                                                                  <  2:496625/140‑1 (MQ=255)                   tcgtcgTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCC‑CCACCGATGGCAGCCCGGCGCTCTTTGCCTCAAc                                                                                                             <  2:112579/140‑1 (MQ=255)                    cgtcgtCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAAc                                                                                                             >  2:777525/1‑140 (MQ=255)                      tcgtcTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACAt                                                                                                           >  1:499017/1‑140 (MQ=255)                         tcTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGctct                                                                                                                       >  1:269595/1‑125 (MQ=255)                         tcTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGctct                                                                                                                       <  2:269595/125‑1 (MQ=255)                         tcTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGc                                                                                                        >  1:668004/1‑140 (MQ=255)                          cTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGcc                                                                                                       <  1:460851/140‑1 (MQ=255)                          cTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGcc                                                                                                       <  2:668004/140‑1 (MQ=255)                          cTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGcc                                                                                                       <  2:138765/140‑1 (MQ=255)                                  gcgcTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGGGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGttt                                                                                               >  1:437899/1‑140 (MQ=255)                                     ctcgctGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATg                                                                                            <  2:947305/140‑1 (MQ=255)                                     ctcgctGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATg                                                                                            <  2:499017/140‑1 (MQ=255)                                     ctcgctGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATg                                                                                            <  2:755956/140‑1 (MQ=255)                                     ctcgctGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATg                                                                                            <  1:777525/140‑1 (MQ=255)                                              aGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGGGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAg                                                                                   <  2:437899/140‑1 (MQ=255)                                               gccgccAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGa                                                                                  >  2:600284/1‑140 (MQ=255)                                                  gccAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATgg                                                                               <  2:899402/140‑1 (MQ=255)                                                  gccAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATgg                                                                               >  2:649391/1‑140 (MQ=255)                                                    cAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCg                                                                             <  1:584676/140‑1 (MQ=255)                                                      gagaAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTg                                                                           <  2:124832/140‑1 (MQ=255)                                                        gaAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGAt                                                                         <  1:649391/140‑1 (MQ=255)                                                         aaGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATg                                                                        <  1:311690/140‑1 (MQ=255)                                                           gCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCg                                                                             >  1:861577/1‑133 (MQ=255)                                                           gCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCg                                                                             <  2:861577/133‑1 (MQ=255)                                                                 cgTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGAt                                                                >  2:754043/1‑140 (MQ=255)                                                                         tGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGc                                                        <  2:866474/140‑1 (MQ=255)                                                                                 cGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGCTTGCCGGa                                                <  1:807557/140‑1 (MQ=255)                                                                                 cGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGCTTGCCGGa                                                <  2:23179/140‑1 (MQ=255)                                                                                    ccTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTCCCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGCTTGCCGGAGTc                                             <  1:6968/140‑1 (MQ=255)                                                                                        tGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGCTTGCCGGAGTCGGtt                                         >  1:686631/1‑140 (MQ=255)                                                                                          cgcgATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGCTTGCCGGAg                                               >  2:495177/1‑132 (MQ=255)                                                                                          cgcgATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGCTTGCCGGAg                                               <  1:495177/132‑1 (MQ=255)                                                                                              aTTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGCTTGCCGGAGTCGGTTTACCCg                                   >  1:456108/1‑140 (MQ=255)                                                                                              aTTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGCTTGCCGGAGTCGGTTTACCCg                                   >  1:318202/1‑140 (MQ=255)                                                                                                 gctgctGGAACGTCGGGGCGTATTCCCCGCCCCCGATGGCAGCCCGGCGCTTTTTGCCTCAACACCGCCGGGCGTTTATGGGGGCGTAGAGGGCGTGAGGCGGGTGATCGAACAGCTTGCCGGAGTCGGTTTACCCGCCt                                <  2:318202/140‑1 (MQ=255)                                                                                                     ggggACCGTCGGGGGTTATTCCCCGCCCCCGAGGGCAGCCCGGCGCTTTTTCCCCAAACACCGCCGGGGGTTTATGGGGGGGTAGATGGGGTGAGGCGGGTGATCGAACAGCTTGCGGGAGTCGTTTTACCCGCCTCAct                            <  2:456108/138‑1 (MQ=255)                                                                                                               aGGGCGTATTCCCCGCCACCGATGGCAGCCGGGCGCTCTTTGCCTCAACACCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGCTTGCCGGAGTCGGTTTACCCGCCTCACTCTGGGAAAGc                  <  1:512197/140‑1 (MQ=255)                                                                                                                     ttttccccGCCACCGAGGGCAGCCGGGCGCTTTTTGCCTCAACATCGCCGGGCGTTTAGGGGGGCGTAGAGGGGGTGAGGGGGGTGATCGAACAGCTGGCGGGAGTCGGTTTACCCGCCTCACTCTGGGAAAGCCAGATc            <  2:686631/135‑1 (MQ=255)                                                                                                                      aTTACCCGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGCTTGCCGGAGTCGGTTTACCCGCCTCACTCTGGGAAAGCCAGATcc           >  1:747976/1‑140 (MQ=255)                                                                                                                            cGCCACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGCTTGCCGGAGTCGGTTTACCCGCCTCACTCTGGGAAAGCCAGATcctgcctg     >  1:165742/1‑140 (MQ=255)                                                                                                                              ccACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGCTTGCCGGAGTCGGTTTACCCGCCTCACTCTGGGAAAGCCAGATCCtgcctgcc   >  2:333262/1‑140 (MQ=255)                                                                                                                               ccccGAGGGCAGCCGGGCGCTTTTTGCCTCAACACCGCCGGGCGTTTATGAGGGCGTAGAGGGCGTGAGGCGGGTGATCGAACAGCTGGCCGGGGTCGGTTTCCCCGCCTCACTCTGGGAAAGCCAGATCCTGCCTGccc  <  2:110699/138‑1 (MQ=255)                                                                                                                               cACCGATGGCAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGCTTGCCGGAGTCGGTTTACCCGCCTCACTCTGGGAAAGCCAGATCCTGCCTGccc  >  1:110699/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                   GGTGAGCGATGAAGTATTTCGTCGTCTGCGTTTGCGCTCGCTGCAAGCCGCCAGAGAAGCGACGCGTCCCGTTGCAGCCACGACCTATGCGCGATTGCTGCTGGAACGTCAGGGCGTATTACCCGCCACCGATGGTAGCCCGGCGCTCTTTGCCTCAACATCGCCAGGCGTTTATGAGGGCGTAGATGGCGTGATGCGGGTGATCGAACAGCTTGCCGGAGTCGGTTTACCCGCCTCACTCTGGGAAAGCCAGATCCTGCCTGCCC  >  NC_000913/1732311‑1732576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑