Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	206,603	C→T	A493V (GCG→GTG)	dnaE →	DNA polymerase III subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	206,603	0	C	T	100.0%	162.1 / NA	48	A493V (GCG→GTG)	dnaE	DNA polymerase III subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/27);  total (21/27)

GTGCTGGGGCATCCGTACGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGCTGCCGGAAATCTACGAAGCGGATGAAGAAGTTAAGGCGCTGATCGACATGGCGCGCAAACTGGAAGGGGTCACCCGTAACGCCGGTAAGCACGCCGGTGGGGTGGTTATCGCGCCGACCAAAATTACCGATTTTGCGCCGCTTTACTGCGATGAAGAGGGCAAACATCCGGT  >  NC_000913/206467‑206738                                                                                                                                         |                                                                                                                                        gTGCTGGGGCATCCGTACGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGCTGCCGGAAATCTACGAAGCGGATGAAGAAGTTAAGGTGCt                                                                                                                                      <  2:121245/140‑1 (MQ=255) gTGCTGGGGCATCCGTACGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGCTGCCGGAAATCTACGAAGCGGATGAAGAAGTTAAGGTGCt                                                                                                                                      <  1:318116/140‑1 (MQ=255) gTGCTGGGGCATCCGTACGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGCTGCCGGAAATCTACGAAGCGGATGAAGAAGTTAAGGTGCt                                                                                                                                      <  2:871692/140‑1 (MQ=255)       gggCATCCGTACGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGCTGCCGGAAATCTACGAAGCGGATGAAGAAGTTAAGGTGCTGATCGa                                                                                                                                >  1:842586/1‑140 (MQ=255)            tCCGTACGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGCTGCCGGAAATCTACGAAGCGGATGAAGAAGTTAAGGTGCTGATCGACATgg                                                                                                                           <  1:863833/140‑1 (MQ=255)             ccGTACGGCTTCGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGCTGCCGGAAATCTACGAAGCGGATGAAGAAGTTAAGGTGCTGATCGACATGgc                                                                                                                          >  1:508641/1‑140 (MQ=255)                 aCGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGCTGCCGGAAATCTACGAAGCGGATGAAGAAGTTAAGGTGCTGATCGACATGgcgcgc                                                                                                                      >  2:316198/1‑140 (MQ=255)                     cTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGCTGCCGGAAATCTACGAAGCGGATGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAAc                                                                                                                  >  2:774819/1‑140 (MQ=255)                        tGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGCTGCCGGAAATCTACGAAGCGGATGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAACTgg                                                                                                               >  1:344217/1‑140 (MQ=255)                        tGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGCTGCCGGAAATCTACGAAGCGGATGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAACTgg                                                                                                               <  1:630878/140‑1 (MQ=255)                          tCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGCTGCCGGAAATCTACGAAGCGGATGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAACTGGaa                                                                                                             <  1:461560/140‑1 (MQ=255)                             aTCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGCTGCCGGAAATCTACGAAGCGGATGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAACTGGAAggg                                                                                                          >  2:170390/1‑140 (MQ=255)                               cGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGCTGCCGGAAATCTACGAAGCGGATGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAACTGGAAGGGGt                                                                                                        <  2:239129/140‑1 (MQ=255)                               cGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGCTGCCGGAAATCTACGAAGCGGATGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAACTGGAAGGGGt                                                                                                        >  1:878330/1‑140 (MQ=255)                                gTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGCTGCCGGAAATCTACGAAGCGGATGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAACTGGAAGGGGTc                                                                                                       <  2:344217/140‑1 (MQ=255)                                    ctcGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGCTGCCGGAAATCTACGAAGCGGATGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAACTGGAAGGGGTCAccc                                                                                                   >  1:465451/1‑140 (MQ=255)                                     tcGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGCTGCCGGAAATCTACGAAGCGGATGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAACTGGAAGGGGTCACCCg                                                                                                  <  1:170390/140‑1 (MQ=255)                                       gAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGCTGCCGGAAATCTACGAAGCGGATGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAACTGGAAGGGGTCACCCGTa                                                                                                <  2:465451/140‑1 (MQ=255)                                        aaaCTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGCTGCCGGAAATCTACGAAGCGGATGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAACTGGAAGGGGTCACCCGTaa                                                                                               >  1:91931/1‑140 (MQ=255)                                          aCTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGCTGCCGGAAATCTACGAAGCGGATGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAACTGGAAGGGGTCACCCGTAACg                                                                                             <  1:222093/140‑1 (MQ=255)                                          aCTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGCTGCCGGAAATCTACGAAGCGGATGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAACTGGAAGGGGTCACCCGTAACg                                                                                             <  2:508641/140‑1 (MQ=255)                                              aTCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGCTGCCGGAAATCTACGAAGCGGATGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAACTGGAAGGGGTCACCCGTAACGCCgg                                                                                         >  2:838218/1‑140 (MQ=255)                                                   gcccgATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGCTGCCGGAAATCTACGAAGCGGATGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAACTGGAAGGGGTCACCCGTAACGCCGGTAAGc                                                                                    >  2:358484/1‑140 (MQ=255)                                                    cccgATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGCTGCCGGAAATCTACGAAGCGGATGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAACTGGAAGGGGTCACCCGTAACGCCGGTAAGCa                                                                                   <  1:838218/140‑1 (MQ=255)                                                    cccgATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGCTGCCGGAAATCTACGAAGCGGATGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAACTGGAAGGGGTCACCCGTAACGCCGGTAAGCa                                                                                   <  2:113784/140‑1 (MQ=255)                                                    cccgATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGCTGCCGGAAATCTACGAAGCGGATGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAACTGGAAGGGGTCACCCGTAACGCCGGTAAGCa                                                                                   <  2:91931/140‑1 (MQ=255)                                                     ccgATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGCTGCCGGAAATCTACGAAGCGGATGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAACTGGAAGGGGTCACCCGTAACGCCGGTAAGCAc                                                                                  >  2:646903/1‑140 (MQ=255)                                                     ccgATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGCTGCCGGAAATCTACGAAGCGGATGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAACTGGAAGGGGTCACCCGTAACGCCGGTAAGCAc                                                                                  >  2:807329/1‑140 (MQ=255)                                                             gggATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGCTGCCGGAAATCTACGAAGCGGATGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAACTGGAAGGGGTCACCCGTAACGCCGGTAAGCACGCCggtgg                                                                          <  1:445277/140‑1 (MQ=255)                                                                aTGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGCTGCCGGAAATCTACGAAGCGGATGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAACTGGAAGGGGTCACCCGTAACGCCGGTAAGCACGCCggtggggt                                                                       <  1:807329/140‑1 (MQ=255)                                                                   aCGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGCTGCCGGAAATCTACGAAGCGGATGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAACTGGAAGGGGTCACCCGTAACGCCGGTAAGCACGCCGGTGGggtggt                                                                    >  2:447882/1‑140 (MQ=255)                                                                        ggCGAAAGCGTTTGAAGCCGAGCCGCAGCTGCCGGAAATCTACGAAGCGGATGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAACTGGAAGGGGTCACCCGTAACGCCGGTAAGCACGCCGGTGGGGTGGTTATcg                                                               >  2:743887/1‑140 (MQ=255)                                                                                                gCAGCTGCCGGAAATCTACGAAGCGGATGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAACTGGAAGGGGTCACCCGTAACGCCGGTAAGCACGCCGGTGGGGTGGTTATCGCGCCGACCAAAATTACCGATTTTg                                       <  1:924453/140‑1 (MQ=255)                                                                                                  aGCTGCCGGAAATCTACGAAGCGGATGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAACTGGAAGGGGTCACCCGTAACGCCGGTAAGCACGCCGGTGGGGTGGTTATCGCGCCGACCAAAATTACCGATTTTgcg                                     <  1:135786/140‑1 (MQ=255)                                                                                                          gAAATCTACGAAGCGGATGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAACTGGAAGGGGTCACCCGTAACGCCGGTAAGCACGCCGGTGGGGTGGTTATCGCGCCGACCAAAATTACCGATTTTGCGCCGCTTTa                             <  2:842586/140‑1 (MQ=255)                                                                                                                  cGAAGCGGATGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAACTGGAAGGGGTCACCCGTAACGCCGGTAAGCACGCCGGTGGGGTGGTTATCGCGCCGACCAAAATTACCGATTTTGCGCCGCTTTACTGCGATg                     <  1:774819/140‑1 (MQ=255)                                                                                                                    aaGCGGATGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAACTGGAAGGGGTCACCCGTAACGCCGGTAAGCACGCCGGTGGGGTGGTTATCGCGCCGACCAAAATTACCGATTTTGCGCCGCTTTACTGCGATGaa                   <  1:686581/140‑1 (MQ=255)                                                                                                                     aGCGGATGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAACTGGAAGGGGTCACCCGTAACGCCGGTAAGCACGCCGGTGGGGTGGTTATCGCGCCGACCAAAATTACCGATTTTGCGCCGCTTTACTGCGATGAag                  >  2:521546/1‑140 (MQ=255)                                                                                                                       cGGATGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAACTGGAAGGGGTCACCCGTAACGCCGGTAAGCACGCCGGTGGGGTGGTTATCGCGCCGACCAAAATTACCGATTTTGCGCCGCTTTACTGCGATg                     >  1:793249/1‑135 (MQ=255)                                                                                                                       cGGATGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAACTGGAAGGGGTCACCCGTAACGCCGGTAAGCACGCCGGTGGGGTGGTTATCGCGCCGACCAAAATTACCGATTTTGCGCCGCTTTACTGCGATg                     <  2:793249/135‑1 (MQ=255)                                                                                                                         gATGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAACTGGAAGGGGTCACCCGTAACGCCGGTAAGCACGCCGGTGGGGTGGTTATCGCGCCGACCAAAATTACCGATTTTGCGCCGCTTTACTGCGATGAAGAggg              >  2:684895/1‑140 (MQ=255)                                                                                                                          aTGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAACTGGAAGGGGTCACCCGTAACGCCGGTAAGCACGCCGGTGGGGTGGTTATCGCGCCGACCAAAATTACCGATTTTGCGCCGCTTTACTGCGATGAAGAGGGc             >  1:817077/1‑140 (MQ=255)                                                                                                                          aTGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAACTGGAAGGGGTCACCCGTAACGCCGGTAAGCACGCCGGTGGGGTGGTTATCGCGCCGACCAAAATTACCGATTTTGCGCCGCTTTACTGCGATGAAGAGGGc             >  1:469471/1‑140 (MQ=255)                                                                                                                          aTGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAACTGGAAGGGGTCACCCGTAACGCCGGTAAGCACGCCGGTGGGGTGGTTATCGCGCCGACCAAAATTACCGATTTTGCGCCGCTTTACTGCGATGAAGAGGGc             <  1:447882/140‑1 (MQ=255)                                                                                                                           tGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAACTGGAAGGGGTCACCCGTAACGCCGGTAAGCACGCCGGTGGGGTGGTTATCGCGCCGACCAAAATTACCGATTTTGCGCCGCTTTACTGCGATGAAGAGGGCa            <  2:469471/140‑1 (MQ=255)                                                                                                                           tGAAGAAGTTAAGGTGCTGATCGACATGGCGCGCAAACTGGAAGGGGTCACCCGTAACGCCGGTAAGCACGCCGGTGGGGTGGTTATCGCGCCGACCAAAATTACCGATTTTGCGCCGCTTTACTGCGATGAAGAGGGCa            <  2:817077/140‑1 (MQ=255)                                                                                                                                   ttAAGGTGCTGATCGACATGGCGCGCAAACTGGAAGGGGTCACCCGTAACGCCGGTAAGCACGCCGGTGGGGCGGTTATCGCGCCGACCAAAATTACCGATTTTGCGCCGCTTTACTGCGATGAAGAGGGCAAACATCCg    >  1:116348/1‑140 (MQ=255)                                                                                                                                     aaGGTGCTGATCGACATGGCGCGCAAACTGGAAGGGGTCACCCGTAACGCCGGTAAGCACGCCGGTGGGGTGGTTATCGCGCCGACCAAAATTACCGATTTTGCGCCGCTTTACTGCGATGAAGAGGGCAAACATCCGGt  <  1:868689/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        GTGCTGGGGCATCCGTACGGCTTTGTCGATCGTATCTCGAAACTGATCCCGCCCGATCCGGGGATGACGCTGGCGAAAGCGTTTGAAGCCGAGCCGCAGCTGCCGGAAATCTACGAAGCGGATGAAGAAGTTAAGGCGCTGATCGACATGGCGCGCAAACTGGAAGGGGTCACCCGTAACGCCGGTAAGCACGCCGGTGGGGTGGTTATCGCGCCGACCAAAATTACCGATTTTGCGCCGCTTTACTGCGATGAAGAGGGCAAACATCCGGT  >  NC_000913/206467‑206738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑