Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,847,618	G→A	Q131* (CAG→TAG)	selD ←	selenide, water dikinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,847,618	0	G	A	100.0%	112.9 / NA	33	Q131* (CAG→TAG)	selD	selenide, water dikinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/16);  total (17/16)

TTGCATCCGGCTTGTGCGGTACTGTTTTTCTTCACCCGCTCGGTCGGTACGATCCCCGTTACCGCCAGACCAAAAATCGGCTCCGGCGCATCGATGGAGTGACCGCCAGCCAGCGCAATACCCGCCTGACGACATGCATAGCGTCCACCTTCGGTCACTTCGCGGGCAATTTCTGGGGAAAGTTTGTTAATCGGCCAGCCGAGGATCGCAATCGCCATAATCGGTTTGCCCCCCATCGCGAAGATATCGCTGATGGCGTTAGT  >  NC_000913/1847491‑1847753                                                                                                                                |                                                                                                                                        ttGCATCCGGCTTGTGCGGTACTGTTTTTCTTCACCCGCTCGGTCGGTACGATCCCCGTTACCGCCAGACCAAAAATCGGCTCCGGCGCATCGATGGAGTGACCGCCAGCCAGCGCAATACCCGCCTAACGACATGCATa                                                                                                                             <  1:682263/140‑1 (MQ=255)  tGCATCCGGCTTGTGCGGTACTGTTTTTCTTCACCCGCTCGGTCGGTACGATCCCCGTTACCGCCAGACCAAAAATCGGCTCCGGCGCATCGATGGAGTGACCGCCAGCCAGCGCAATACCCGCCTAACGACATGCATAg                                                                                                                            >  2:592537/1‑140 (MQ=255)    cATCCGGCTTGTGCGGTACTGTTTTTCTTCACCCGCTCGGTCGGTACGATCCCCGTTACCGCCAGACCAAAAATCGGCTCCGGCGCATCGATGGAGTGACCGCCAGCCAGCGCAATACCCGCCTAACGACATGCATAGCg                                                                                                                          >  1:203982/1‑140 (MQ=255)       ccGGCTTGTGCGGTACTGTTTTTCTTCACCCGCTCGGTCGGTACGATCCCCGTTACCGCCAGACCAAAAATCGGCTCCGGCGCATCGATGGAGTGACCGCCAGCCAGCGCAATACCCGCCTAACGACATGCATAGCGTcc                                                                                                                       >  1:682770/1‑140 (MQ=255)        cGGCTTGTGCGGTACTGTTTTTCTTCACCCGCTCGGTCGGTACGATCCCCGTTACCGCCAGACCAAAAATCGGCTCCGGCGCATCGATGGAGTGACCGCCAGCCAGCGCAATACCCGCCTAACGACATGCATAGCGTCCa                                                                                                                      >  2:593690/1‑140 (MQ=255)                              cttcACCCGCTCGGTCGGTACGATCCCCGTTACCGCCAGACCAAAAATCGGCTCCGGCGCATCGATGGAGTGACCGCCAGCCAGCGCAATACCCGCCTAACGACATGCATAGCGTCCACCTTCGGTCACTTCGCGGGCaa                                                                                                >  1:630087/1‑140 (MQ=255)                              cttcACCCGCTCGGTCGGTACGATCCCCGTTACCGCCAGACCAAAAATCGGCTCCGGCGCATCGATGGAGTGACCGCCAGCCAGCGCAATACCCGCCTAACGACATGCATAGCGTCCACCTTCGGTCACTTCGCGGGCaa                                                                                                >  1:798369/1‑140 (MQ=255)                                tcACCCGCTCGGTCGGTACGATCCCCGTTACCGCCAGACCAAAAATCGGCTCCGGCGCATCGATGGAGTGACCGCCAGCCAGCGCAATACCCGCCTAACGACATGCATAGCGTCCACCTTCGGTCACTTCGCGGGCAAtt                                                                                              >  2:442737/1‑140 (MQ=255)                                 cACCCGCTCGGTCGGTACGATCCCCGTTACCGCCAGACCAAAAATCGGCTCCGGCGCATCGATGGAGTGACCGCCAGCCAGCGCAATACCCGCCTAACGACATGCATAGCGTCCACCTTCGGTCACTTCGCGGGCAAttt                                                                                             >  1:933972/1‑140 (MQ=255)                                     cGCTCGGTCGGTACGATCCCCGTTACCGCCAGACCAAAAATCGGCTCCGGCGCATCGATGGAGTGACCGCCAGCCAGCGCAATACCCGCCTAACGACATGCATAGCGTCCACCTTCGGTCACTTCGCGGGCAATTTCTgg                                                                                         <  1:592537/140‑1 (MQ=255)                                      gCTCGGTCGGTACGATCCCCGTTACCGCCAGACCAAAAATCGGCTCCGGCGCATCGATGGAGTGACCGCCAGCCAGCGCAATACCCGCCTAACGACATGCATAGCGTCCACCTTCGGTCACTTCGCGGGCAATTTCTggg                                                                                        <  2:169116/140‑1 (MQ=255)                                         cggtcggtACGATCCCCGTTACCGCCAGACCAAAAATCGGCTCCGGCGCATCGATGGAGTGACCGCCAGCCAGCGCAATACCCGCCTAACGACATGCATAGCGTCCACCTTCGGTCACTTCGCGGGCAATTTCTGGGGaa                                                                                     <  2:798369/140‑1 (MQ=255)                                                      ccccGTTACCGCCAGACCAAAAATCGGCTCCGGCGCATCGATGGAGTGACCGCCAGCCAGCGCAATACCCGCCTAACGACATGCATAGCGTCCACCTTCGGTCACTTCGCGGGCAATTTCTggg                                                                                        <  2:939440/124‑1 (MQ=255)                                                      ccccGTTACCGCCAGACCAAAAATCGGCTCCGGCGCATCGATGGAGTGACCGCCAGCCAGCGCAATACCCGCCTAACGACATGCATAGCGTCCACCTTCGGTCACTTCGCGGGCAATTTCTggg                                                                                        >  1:939440/1‑124 (MQ=255)                                                        ccGTTACCGCCAGACCAAAAATCGGCTCCGGCGCATCGATGGAGTGACCGCCAGCCAGCGCAATACCCGCCTAACGACATGCATAGCGTCCACCTTCGGTCACTTCGCGGGCAATTTCTGGGGAAAGTTTGTTAATCGGc                                                                      <  2:219091/140‑1 (MQ=255)                                                        ccGTTACCGCCAGACCAAAAATCGGCTCCGGCGCATCGATGGAGTGACCGCCAGCCAGCGCAATACCCGCCTAACGACATGCATAGCGTCCACCTTCGGTCACTTCGCGGGCAATTTCTGGGGAAAGTTTGTTAATCGGc                                                                      <  2:682770/140‑1 (MQ=255)                                                             aCCGCCAGACCAAAAATCGGCTCCGGCGCATCGATGGAGTGACCGCCAGCCAGCGCAATACCCGCCTAACGACATGCATAGCGTCCACCTTCGGTCACTTCGCGGGCAATTTCTGGGGAAAGTTTGTTAATCGGCCAGcc                                                                 <  2:203982/140‑1 (MQ=255)                                                               cGCCAGACCAAAAATCGGCTCCGGCGCATCGATGGAGTGACCGCCAGCCAGCACAATACCCGCCTAACGACATGCATAGCGTCCACCTTCGGTCACTTCGCGGGCAATTTCTGGGGAAAGTTTGTTAATCGGCCAGCCGa                                                               <  1:387285/140‑1 (MQ=255)                                                                     aCCAAAAATCGGCTCCGGCGCATCGATGGAGTGACCGCCAGCCAGCGCAATACCCGCCTAACGACATGCATAGCGTCCACCTTCGGTCACTTCGCGGGCAATTTCTGGGGAAAGTTTGTTAATCGGCCAGc                                                                  <  1:932611/131‑1 (MQ=255)                                                                     aCCAAAAATCGGCTCCGGCGCATCGATGGAGTGACCGCCAGCCAGCGCAATACCCGCCTAACGACATGCATAGCGTCCACCTTCGGTCACTTCGCGGGCAATTTCTGGGGAAAGTTTGTTAATCGGCCAGc                                                                  >  2:932611/1‑131 (MQ=255)                                                                                gCTCCGGCGCATCGATGGAGTGACCGCCAGCCAGCGCAATACCCGCCTAACGACATGCATAGCGTCCACCTTCGGTCACTTCGCGGGCAATTTCTGGGGAAAGTTTGTTAATCGGCCAGCCGAGGATCGCAATCGCCATa                                              >  1:380139/1‑140 (MQ=255)                                                                                gCTCCGGCGCATCGATGGAGTGACCGCCAGCCAGCGCAATACCCGCCTAACGACATGCATAGCGTCCACCTTCGGTCACTTCGCGGGCAATTTCTGGGGAAAGTTTGTTAATCGGCCAGCCGAGGATCGCAATCGCCATa                                              >  2:805686/1‑140 (MQ=255)                                                                                   ccGGCGCATCGATGGAGTGACCGCCAGCCAGCGCAATACCCGCCTAACGACATGCATAGCGTCCACCTTCGGTCACTTCGCGGGCAATTTCTGGGGAAAGTTTGTTAATCGGCCAGCCGAGGATCGCAATCGCCATAATc                                           <  1:805686/140‑1 (MQ=255)                                                                                    cGGCGCATCGTTGGAGTGACCGCCAGCCAGCGCAATACCCGCCTAACGACATGCATAGCGTCCACCTTCGGTCACTTCGCGGGCAATTTCTGGGGAAAGTTTGTTAATCGGCCAGCCGAGGATCGCAATCGCCATAATCg                                          <  2:854442/140‑1 (MQ=255)                                                                                      gcgcATCGATGGAGTGACCGCCAGCCAGCGCAATACCCGCCTAACGACATGCATAGCGTCCACCTTCGGTCACTTCGCGGGCAATTTCTGGGGAAAGTTTGTTAATCGGCCAGCCGAGGATCGCAATCGCCATAATCGGt                                        <  2:933972/140‑1 (MQ=255)                                                                                               tGGAGTGACCGCCAGCCAGCGCAATACCCGCCTAACGACATGCATAGCGTCCACCTTCGGTCACTTCGCGGGCAATTTCTGGGGAAAGTTTGTTAATCGGCCAGCCGAGGATCGCAATCGCCATAATCGGTTTGcccccc                               <  1:442737/140‑1 (MQ=255)                                                                                                      aCCGCCAGCCAGCGCAATACCCGCCTAACGACATGCATAGCGTCCACCTTCGGTCACTTCGCGGGCAATTTCTGGGGAAAGTTTGTTAATCGGCCAGCCGAGGATCGCAATCGCCATAATCGGTTTGCCCCCCATcgc                          <  1:258153/138‑1 (MQ=255)                                                                                                      aCCGCCAGCCAGCGCAATACCCGCCTAACGACATGCATAGCGTCCACCTTCGGTCACTTCGCGGGCAATTTCTGGGGAAAGTTTGTTAATCGGCCAGCCGAGGATCGCAATCGCCATAATCGGTTTGCCCCCCATcgc                          >  2:258153/1‑138 (MQ=255)                                                                                                      aCCGCCAGCCAGCGCAATACCCGCCTAACGACATGCATAGCGTCCACCTTCGGTCACTTCGCGGGCAATTTCTGGGGAAAGTTTGTTAATCGGCCAGCCGAGGATCGCAATCGCCATAATCGGTTTGCCCCCCATCGCGa                        >  2:914087/1‑140 (MQ=255)                                                                                                                  cgcAATACCCGCCTAACGACATGCATAGCGTCCACCTTCGGTCACTTCGCGGGCAATTTCTGGGGAAAGTTTGTTAATCGGCCAGCCGAGGATCGCAATCGCCATAATCGGTTTGCCCCCCATCGCGAAGATATCGCTGa            >  2:931944/1‑140 (MQ=255)                                                                                                                  cgcAATACCCGCCTAACGACATGCATAGCGTCCACCTACGGTCACTTCGCGGGCAATTTCTGGGGAAAGTTTGTTAATCGGCCAGCCGAGGATCGCAATGGCCATAATCGGTTGGCCCCCCATCGCGAAGATATGGCTGa            >  2:839972/1‑140 (MQ=255)                                                                                                                            gCCTAACGACATGCATAGCGTCCACCTTCGGTCACTTCGCGGGCAATTTCTGGGGAAAGTTTGTTAATCGGCCAGCCGAGGATCGCAATCGCCATAATCGGTTTGCCCCCCATCGCGAAGATATCGCTGATGGCGTTAGt  <  1:688560/140‑1 (MQ=255)                                                                                                                            gCCTAACGACATGCATAGCGTCCACCTTCGGTCACTTCGCGGGCAATTTCTGGGGAAAGTTTGTTAATCGGCCAGCCGAGGATCGCAATCGCCATAATCGGTTTGCCCCCCATCGCGAAGATATCGCTGATGGCGTTAGt  >  1:863555/1‑140 (MQ=255)                                                                                                                                |                                                                                                                                        TTGCATCCGGCTTGTGCGGTACTGTTTTTCTTCACCCGCTCGGTCGGTACGATCCCCGTTACCGCCAGACCAAAAATCGGCTCCGGCGCATCGATGGAGTGACCGCCAGCCAGCGCAATACCCGCCTGACGACATGCATAGCGTCCACCTTCGGTCACTTCGCGGGCAATTTCTGGGGAAAGTTTGTTAATCGGCCAGCCGAGGATCGCAATCGCCATAATCGGTTTGCCCCCCATCGCGAAGATATCGCTGATGGCGTTAGT  >  NC_000913/1847491‑1847753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑