| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,979,911 | G→A | M53I (ATG→ATA) | uspC → | universal stress protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,979,911 | 0 | G | A | 98.1% | 174.8 / ‑4.8 | 52 | M53I (ATG→ATA) | uspC | universal stress protein C |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (17/34); total (17/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
CGCTGTTGCGGTTACACCGGAAAGTCAGCAACTGCTGGCAAAAGCGGTATCTATCGCCAGGCCAGTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATGCTTGAAGATTTACGCAGCGTGATGCATGAAGAAACGCAAAGCTTTCTTGATAAGTTAATTCAGGATGCGGGGTATCCCGTTGACAAGACGTTTATTGCCTACGGTGAGTTAAGCGAACATATTCTGGAA > NC_000913/1979776‑1980040 | cGCTGTTGCGGTTACACCGGAAAGTCAGCAACTGCTGGCAAAAGCGGTATCTATCGCCAGGCCAGTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATACTTg < 2:84878/140‑1 (MQ=255) tGTTGCGGTTACACCGGAAAGTCAGCAACTGCTGGCAAAAGCGGTATCTATCGCCAGGCCAGTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATACTTGAAg > 2:805008/1‑140 (MQ=255) cGGTTACACCGGAAAGTCAGCAACTGCTGGCAAAAGCGGTATCTATCGCCAGGCCAGTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATACTTGAAGATTTa > 1:9864/1‑140 (MQ=255) gTTACACCGGAAAGTCAGCAACTGCTGGCAAAAGCGGTATCTATCGCCAGGCCAGTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATACTTGAAGATTTACg > 1:29405/1‑140 (MQ=255) gTTACACCGGAAAGTCAGCAACTGCTGGCAAAAGCGGTATCTATCGCCAGGCCAGTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATACTTGAAGATTTACg < 1:940112/140‑1 (MQ=255) ttACACCGGAAAGTCAGCAACTGCTGGCAAAAGCGGTATCTATCGCCAGGCCAGTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATACTTGAAGATTTACGc < 1:389589/140‑1 (MQ=255) tACACCGGAAAGTCAGCAACTGCTGGCAAAAGCGGTATCTATCGCCAGGCCAGTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATACTTGAAGATTTACGCa < 2:566575/140‑1 (MQ=255) tACACCGGAAAGTCAGCAACTGCTGGCAAAAGCGGTATCTATCGCCAGGCCAGTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATACTTGAAGATTTACGCa < 1:528527/140‑1 (MQ=255) acacCGGAAAGTCAGCAACTGCTGGCAAAAGCGGTATCTATCGCCAGGCCAGTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATACTTGAAGATTTACGCAg < 2:293600/140‑1 (MQ=255) acCGGAAAGTCAGCAACTGCTGGCAAAAGCGGTATCTATCGCCAGGCCAGTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATACTTGAAGATTTACGCAGCg < 1:408186/140‑1 (MQ=255) ccGGAAAGTCAGCAACTGCTGGCAAAAGCGGTATCTATCGCCAGGCCAGTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATGCTTGAAGATTTACGCAGCGt < 1:918619/140‑1 (MQ=255) ccGGAAAGTCAGCAACTGCTGGCAAAAGCGGTATCTATCGCCAGGCCAGTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATACTTGAAGATTTACGCAGCGt < 2:415953/140‑1 (MQ=255) cGGAAAGTCAGCAACTGCTGGCAAAAGCGGTATCTATCGCCAGGCCAGTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATACTTGAAGATTTACGCAGCGTg < 1:801345/140‑1 (MQ=255) cGGAAAGTCAGCAACTGCTGGCAAAAGCGGTATCTATCGCCAGGCCAGTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATACTTGAAGATTTACGCAGCGTg < 2:558296/140‑1 (MQ=255) cGGAAAGTCAGCAACTGCTGGCAAAAGCGGTATCTATCGCCAGGCCAGTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATACTTGAAGATTTACGCAGCGTg < 1:805008/140‑1 (MQ=255) gAAAGTCAGCAACTGCTGGCAAAAGCGGTATCTATCGCCAGGCCAGTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATACTTGAAGATTTACGCa < 2:240782/133‑1 (MQ=255) gAAAGTCAGCAACTGCTGGCAAAAGCGGTATCTATCGCCAGGCCAGTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATACTTGAAGATTTACGCa > 1:240782/1‑133 (MQ=255) aaGCGGTATCTATCGCCAGGCCAGTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATACTTGAAGATTTACGCAGCGTGATGCATGAAGAAACGCAAAGCttt < 2:584797/140‑1 (MQ=255) gTATCTATCGCCAGGCCAGTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATACTTGAAGATTTACGCAGCGTGATGCATGAAGAAACGCAAAGCTTTCTTGa < 1:808398/140‑1 (MQ=255) tatctatcGCCAGGCCAGTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATACTTGAAGATTTACGCAGCGTGATGCATGAAGAAACGCAAAGCTTTCTTGAt < 1:321922/140‑1 (MQ=255) atcGCCAGGCCAGTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATACTTGAAGATTTACGCAGCGTGATGCATGAAGAAACGCAAAGCTTTCtt < 2:685621/132‑1 (MQ=255) atcGCCAGGCCAGTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATACTTGAAGATTTACGCAGCGTGATGCATGAAGAAACGCAAAGCTTTCtt > 1:685621/1‑132 (MQ=255) atcGCCAGGCCAGTAAAGGAACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATACTTGAAGATTTACGCAGCGTGATGCATGAAGAAACGCAAAGCTTTCTTGATAAGtt < 2:399495/140‑1 (MQ=255) ggccagTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATACTTGAAGATTTACGCAGCGTGATGCATGAAGAAACGCAAAGCTTTCTTGATAAGTTAATTCAg > 1:639113/1‑140 (MQ=255) cagTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATACTTGAAGATTTACGCAGCGTGATGCATGAAGAAACGCAAAGCTTTCTTGATAAGTTAATTCAGGAt < 2:307607/140‑1 (MQ=255) cagTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATACTTGAAGATTTACGCAGCGTGATGCATGAAGAAACGCAAAGCTTTCTTGATAAGTTAATTCAGGAt < 2:14168/140‑1 (MQ=255) cagTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATACTTGAAGATTTACGCAGCGTGATGCATGAAGAAACGCAAAGCTTTCTTGATAAGTTAATTCAGGAt < 2:833094/140‑1 (MQ=255) agTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATACTTGAAGATTTACGCAGCGTGATGCATGAAGAAACGCAAAGCTTTCTTGATAAGTTAATTCAGGATg > 1:52744/1‑140 (MQ=255) tAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATACTTGAAGATTTACGCAGCGTGATGCATGAAGAAACGCAAAGCTTTCTTGATAAGTTAATTCAGGATGCg < 2:52744/140‑1 (MQ=255) aaGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATACTTGAAGATTTACGCAGCGTGATGCATGAAGAAACGCAAAGCTTTCTTGATAAGTTAATTCAGGATGCggg < 2:345639/140‑1 (MQ=255) tctcGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATACTTGAAGATTTACGCAGCGTGATGCATGAAGAAACGCAAAGCTTTCTTGATAAGTTAATTCAGGATGCGGGGTATCCCGTTGACAAGACGTTTa < 1:472026/140‑1 (MQ=255) ctcGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATACTTGAAGATTTACGCAGCGTGATGCATGAAGAAACGCAAAGCTTTCTTGATAAGTTAATTCAGGATGCGGGGTATCCCGTTGACAAGACGTTTAt < 2:777355/140‑1 (MQ=255) gCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATACTTGAAGATTTACGCAGCGTGATGCATGAAGAAACGCAAAGCTTTCTTGATAAGTTAATTCAGGATGCGGGGTATCCCGTTGAc > 1:482462/1‑126 (MQ=255) gCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATACTTGAAGATTTACGCAGCGTGATGCATGAAGAAACGCAAAGCTTTCTTGATAAGTTAATTCAGGATGCGGGGTATCCCGTTGAc < 2:482462/126‑1 (MQ=255) gCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATACTTGAAGATTTACGCAGCGTGATGCATGAAGAAACGCAAAGCTTTCTTGATAAGTTAATTCAGGATGCGGGGTATCCCGTTGACAAGACGTTTATTGc < 2:550087/140‑1 (MQ=255) ccGACCCGGAAATGTACAATCAATTAGCTGCGCCGATACTTGAAGATTTACGCAGCGTGATGCATGAAGAAACGCAAAGCTTTCTTGATAAGTTAATTCAGGATGCGGGGTATCCCGTTGACAAGACGTTTATTGc > 2:634943/1‑136 (MQ=255) ccGACCCGGAAATGTACAATCAATTAGCTGCGCCGATACTTGAAGATTTACGCAGCGTGATGCATGAAGAAACGCAAAGCTTTCTTGATAAGTTAATTCAGGATGCGGGGTATCCCGTTGACAAGACGTTTATTGc < 1:634943/136‑1 (MQ=255) ccGACCCGGAAATGTACAATCAATTAGCTGCGCCGATACTTGAAGATTTACGCAGCGTGATGCATGAAGAAACGCAAAGCTTTCTTGATAAGTTAATTCAGGATGCGGGGTATCCCGTTGACAAGACGTTTATTGCCTAc < 2:9864/140‑1 (MQ=255) cGACCCGGAAATGTACAATCAATTAGCTGCGCCGATACTTGAAGATTTACGCAGCGTGATGCATGAAGAAACGCAAAGCTTTCTTGATAAGTTAATTCAGGATGCGGGGTATCCCGTTGACAAGACGTTTATTGCCTACg > 1:135671/1‑140 (MQ=255) ggAAATGTACAATCAATTAGCTGCGCCGATACTTGAAGATTTACGCAGCGTGATGCATGAAGAAACGCAAAGCTTTCTTGATAAGTTAATTCAGGATGCGGGGTATCCCGTTGACAAGACGTTTATTGCCTACGGTGAGt < 1:260498/140‑1 (MQ=255) aTGTACAATCAATTAGCTGCGCCGATACTTGAAGATTTACGCAGCGTGATGCATGAAGAAACGCAAAGCTTTCTTGATAAGTTAATTCAGGATGCGGGGTATCCCGTTGACAAGACGTTTATTGCCTACGGTGAGTTAAg < 2:135671/140‑1 (MQ=255) tcaatTAGCTGCGCCGATACTTGAAGATTTACGCAGCGTGATGCATGAAGAAACGCAAAGCTTTCTTGATAAGTTAATTCAGGATGCGGGGTATCCCGTTGACAAGACGTTTATTGCCTACGGTGAGTTAAGCGAACata > 2:203629/1‑140 (MQ=255) tcaatTAGCTGCGCCGATACTTGAAGATTTACGCAGCGTGATGCATGAAGAAACGCAAAGCTTTCTTGATAAGTTAATTCAGGATGCGGGGTATCCCGTTGACAAGACGTTTATTGCCTACGGTGAGTTAAGCGAACata > 2:142166/1‑140 (MQ=255) atTAGCTGCGCCGATACTTGAAGATTTACGCAGCGTGATGCATGAAGAAACGCAAAGCTTTCTTGATAAGTTAATTCAGGATGCGGGGTATCCCGTTGACAAGACGTTTATTGCCTACGGTGAGTTAAGCGAAc < 2:708845/134‑1 (MQ=255) atTAGCTGCGCCGATACTTGAAGATTTACGCAGCGTGATGCATGAAGAAACGCAAAGCTTTCTTGATAAGTTAATTCAGGATGCGGGGTATCCCGTTGACAAGACGTTTATTGCCTACGGTGAGTTAAGCGAAc > 1:708845/1‑134 (MQ=255) ttAGCTGCGCCGATACTTGAAGATTTACGCAGCGTGATGCATGAAGAAACGCAAAGCTTTCTTGATAAGTTAATTCAGGATGCGGGGTATCCCGTTGACAAGACGTTTATTGCCTACGGTGAGTTAAGCGAACATATTCt > 2:23650/1‑140 (MQ=255) aGCTGCGCCGATACTTGAAGATTTACGCAGCGTGATGCATGAAGAAACGCAAAGCTTTCTTGATAAGTTAATTCAGGATGCGGGGTATCCCGTTGACAAGACGTTTATTGCCTACGGTGAGTTAAGCGAACATATTCTgg < 2:129365/140‑1 (MQ=255) cTGCGCCGATACTTGAAGATTTACGCAGCGTGATGCATGAAGAAACGCAAAGCTTTCTTGATAAGTTAATTCAGGATGCGGGGTATCCCGTTGACAAGACGTTTATTGCCTACGGTGAGTTAAGCGAACATATTCTGGaa > 2:188099/1‑140 (MQ=255) gcgcCGATACTTGAAGATTTACGCAGCGTGATGCATGAAGAAACGCAAAGCTTTCTTGATAAGTTAATTCAGGATGCGGGGTATCCCGTTGACAAGACGTTTATTGCCTACGGTGAGTTAAGCGAACa < 2:125944/128‑1 (MQ=255) gcgcCGATACTTGAAGATTTACGCAGCGTGATGCATGAAGAAACGCAAAGCTTTCTTGATAAGTTAATTCAGGATGCGGGGTATCCCGTTGACAAGACGTTTATTGCCTACGGTGAGTTAAGCGAACa > 1:125944/1‑128 (MQ=255) gcgcCGATACTTGAAGATTTACGCAGCGTGATGCATGAAGAAACGCAAAGCTTTCTTGATAAGTTAATTCAGGATGCGGGGTATCCCGTTGACAAGACGTTTATTGCCTACGGTGAGTTAAGCGAACATATTc > 1:828436/1‑133 (MQ=255) gcgcCGATACTTGAAGATTTACGCAGCGTGATGCATGAAGAAACGCAAAGCTTTCTTGATAAGTTAATTCAGGATGCGGGGTATCCCGTTGACAAGACGTTTATTGCCTACGGTGAGTTAAGCGAACATATTc < 2:828436/133‑1 (MQ=255) | CGCTGTTGCGGTTACACCGGAAAGTCAGCAACTGCTGGCAAAAGCGGTATCTATCGCCAGGCCAGTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATGCTTGAAGATTTACGCAGCGTGATGCATGAAGAAACGCAAAGCTTTCTTGATAAGTTAATTCAGGATGCGGGGTATCCCGTTGACAAGACGTTTATTGCCTACGGTGAGTTAAGCGAACATATTCTGGAA > NC_000913/1979776‑1980040 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |