Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,050,863	A→G	T1976A (ACT→GCT)	yeeJ →	inverse autotransporter adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,050,863	0	A	G	100.0%	305.4 / NA	83	T1976A (ACT→GCT)	yeeJ	inverse autotransporter adhesin
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (45/38);  total (45/38)

TACGGTGACTGCATCTTTCCATAACGGCGTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACACTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCGAAGGTCTCACTGTGTACTTCGCCTTAAAAAGCGGCTCTGCCACATTAACGTCATTAACAGCGGTGACCGAT  >  NC_000913/2050733‑2050988                                                                                                                                   |                                                                                                                              taCGGTGACTGCATCTTTCCATAACGGCGTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTaa                                                                                                                      <  1:98800/140‑1 (MQ=255)  aCGGTGACTGCATCTTTCCATAACGGCGTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAg                                                                                                                     <  1:98297/140‑1 (MQ=255)   cGGTGACTGCATCTTTCCATAACGGCGTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGt                                                                                                                    <  1:538778/140‑1 (MQ=255)     gTGACTGCATCTTTCCATAACGGCGTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTAc                                                                                                                  >  1:768680/1‑140 (MQ=255)     gTGACTGCATCTTTCCATAACGGCGTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTAc                                                                                                                  >  1:607925/1‑140 (MQ=255)     gTGACTGCATCTTTCCATAACGGCGTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTAc                                                                                                                  >  1:490920/1‑140 (MQ=255)       gACTGCATCTTTCCATAACGGCGTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCt                                                                                                                             >  2:230019/1‑127 (MQ=255)        aCTGCATCTTTCCATAACGGCGTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGAttt                                                                                                                        <  2:27713/131‑1 (MQ=255)        aCTGCATCTTTCCATAACGGCGTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGAttt                                                                                                                        >  1:27713/1‑131 (MQ=255)        aCTGCATCTTTCCATAACGGCGTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCtt                                                                                                               >  2:158265/1‑140 (MQ=255)        aCTGCATCTTTCCATAACGGCGTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCtt                                                                                                               >  1:723783/1‑140 (MQ=255)        aCTGCATCTTTCCATAACGGCGTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCtt                                                                                                               >  1:546597/1‑140 (MQ=255)         cTGCATCTTTCCATAACGGCGTAATAATACAGACACAGACAACCGTGAAAGTCACTGACAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTa                                                                                                              >  2:652410/1‑140 (MQ=255)         cTGCATCTTTCCATAACGGCGTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTa                                                                                                              >  1:302011/1‑140 (MQ=255)         cTGCATCTTTCCATAACGGCGTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTa                                                                                                              <  1:158265/140‑1 (MQ=255)          tGCATCTTTCCATAACGGCGTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTaa                                                                                                                      <  1:928325/131‑1 (MQ=255)          tGCATCTTTCCATAACGGCGTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTaa                                                                                                                      >  2:928325/1‑131 (MQ=255)            cATCTTTCCATAACGGCGTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTaaa                                                                                                            >  2:326443/1‑139 (MQ=255)            cATCTTTCCATAACGGCGTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTaaa                                                                                                            <  1:326443/139‑1 (MQ=255)            cATCTTTCCATAACGGCGTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAg                                                                                                           >  1:845382/1‑140 (MQ=255)            cATCTTTCCATAACGGCGTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAg                                                                                                           >  1:654778/1‑140 (MQ=255)            cATCTTTCCATAACGGCGTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAg                                                                                                           >  1:626975/1‑140 (MQ=255)              tCTTTCCATAACGGCGTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGc                                                                                                         <  2:764121/140‑1 (MQ=255)                  tCCATAACGGCGTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCaa                                                                                                       <  1:313715/138‑1 (MQ=255)                  tCCATAACGGCGTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCaa                                                                                                       >  2:313715/1‑138 (MQ=255)                  tCCATAACGGCGTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACg                                                                                                     >  2:509605/1‑140 (MQ=255)                   ccATAACGGCGTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACgg                                                                                                    <  1:379519/140‑1 (MQ=255)                     aTAACGGCGTAACAATTCAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGtt                                                                                                  <  2:152597/140‑1 (MQ=255)                            cGTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCaa                                                                                                       <  1:577914/128‑1 (MQ=255)                            cGTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCaa                                                                                                       >  2:577914/1‑128 (MQ=255)                            cGTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGa                                                                                             <  1:682204/138‑1 (MQ=255)                            cGTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGa                                                                                             >  2:682204/1‑138 (MQ=255)                            cGTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATg                                                                                           >  2:423662/1‑140 (MQ=255)                            cGTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATg                                                                                           >  2:363535/1‑140 (MQ=255)                            cGTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATg                                                                                           >  2:917374/1‑140 (MQ=255)                            cGTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATg                                                                                           >  1:247268/1‑140 (MQ=255)                             gTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATgg                                                                                          <  2:768680/140‑1 (MQ=255)                             gTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATgg                                                                                          <  2:634250/140‑1 (MQ=255)                             gTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATgg                                                                                          <  1:505506/140‑1 (MQ=255)                             gTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATgg                                                                                          <  1:423489/140‑1 (MQ=255)                             gTAACAATACAGACACAGACAACCGTGAAAGTCACCGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATgg                                                                                          <  2:654778/140‑1 (MQ=255)                                       agacacagacaACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAAc                                                                                <  2:490920/140‑1 (MQ=255)                                         acacagacaACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATgg                                                                                          >  2:449400/1‑128 (MQ=255)                                         acacagacaACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATgg                                                                                          <  1:449400/128‑1 (MQ=255)                                         acacagacaACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCt                                                                              >  1:4498/1‑140 (MQ=255)                                         acacagacaACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCt                                                                              >  1:541904/1‑140 (MQ=255)                                              gacaACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCg                                                                         >  2:13186/1‑140 (MQ=255)                                              gacaACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCg                                                                         >  2:734971/1‑140 (MQ=255)                                              gacaACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCg                                                                         >  1:684236/1‑140 (MQ=255)                                               acaACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCGa                                                                        >  2:825215/1‑140 (MQ=255)                                                  aCCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCGAAgg                                                                     <  1:825215/140‑1 (MQ=255)                                                    cGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTCAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCGAAGGtc                                                                   <  2:845382/140‑1 (MQ=255)                                                    cGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCGAAGGtc                                                                   <  2:684236/140‑1 (MQ=255)                                                    cGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCGAAGGtc                                                                   <  1:494541/140‑1 (MQ=255)                                                    cGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCGAAGGtc                                                                   <  2:508056/140‑1 (MQ=255)                                                    cGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCGAAGGtc                                                                   <  2:541904/140‑1 (MQ=255)                                                     gTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCGAAGGtct                                                                  <  1:802057/140‑1 (MQ=255)                                                      tGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCGAAGGtctc                                                                 >  1:857994/1‑140 (MQ=255)                                                       gAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCGAAGGTCTCa                                                                <  2:626975/140‑1 (MQ=255)                                                             cACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCGAAGGTCTCACTgtgt                                                          >  2:131561/1‑140 (MQ=255)                                                              aCTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCGa                                                                        <  2:677962/125‑1 (MQ=255)                                                              aCTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCGa                                                                        >  1:677962/1‑125 (MQ=255)                                                              aCTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCGAAGGTCTCACTGTGTa                                                         >  1:231727/1‑140 (MQ=255)                                                                tGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCGAAGGtc                                                                   <  2:249206/128‑1 (MQ=255)                                                                tGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCGAAGGtc                                                                   >  1:249206/1‑128 (MQ=255)                                                                     aCTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCGAAGGtct                                                                  >  1:913267/1‑124 (MQ=255)                                                                     aCTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCGAAGGtct                                                                  <  2:913267/124‑1 (MQ=255)                                                                          aGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCGAAGGTCTCACTGTGTACTTCGCCTTaaa                                             >  1:211611/1‑140 (MQ=255)                                                                               cGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCGAAGGTCTCACTGTGTACTTCGCCTTAAAAAGCg                                        >  1:908930/1‑140 (MQ=255)                                                                               cGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCGAAGGTCTCACTGTGTACTTCGCCTTAAAAAGCg                                        <  1:917374/140‑1 (MQ=255)                                                                                 cccATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCGAAGGTCTCACTGTGTACTTCGCCTTAAAAAGCGGc                                      >  1:770158/1‑140 (MQ=255)                                                                                 cccATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCGAAGGTCTCACTGTGTACTTCGCCTTAAAAAGCGGc                                      <  1:652410/140‑1 (MQ=255)                                                                                 cccATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCGAAGGTCTCACTGTGTACTTCGCCTTAAAAAGCGGc                                      <  1:509605/140‑1 (MQ=255)                                                                                  ccATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCGAAGGTCTCACTGTGTACTTCGCCTTAAAAAGCGGct                                     <  1:168501/140‑1 (MQ=255)                                                                                     tGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCGAAGGTCTCACTGTGTACTTCGCCTTAAAAAGCGGCTCTg                                  <  2:770158/140‑1 (MQ=255)                                                                                          cTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCGAAGGTCTCACTGTGTACTTCGCCTTAAAAAGCGGCTCTGCcaca                             <  2:546597/140‑1 (MQ=255)                                                                                          cTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCGAAGGTCTCACTGTGTACTTCGCCTTAAAAAGCGGCTCTGCcaca                             <  2:857994/140‑1 (MQ=255)                                                                                                  aTCGCTGATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCGTAGGTCTCACTGTGTACTTCGCCTTAAAAAGCGGCTCTGCCACATTAACGTc                     >  1:167696/1‑140 (MQ=255)                                                                                                        gATCCATCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCGAAGGTCTCACTGTGTACTTCGCCTTAAAAAGCGGCTCTGCCACATTAACGTCATTAAc               >  2:926538/1‑140 (MQ=255)                                                                                                              tCGACTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCGAAGGTCTCACTGTGTACTTCGCCTTAAAAAGCGGCTCTGCCACATTAACGTCATTAACAGCGGt         >  2:770735/1‑140 (MQ=255)                                                                                                                 aCTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCGAAGGTCTCACTGTGTACTTCGCCTTAAAAAGCGGCTCTGCCACATTAACGTCATTAACAGCGGTGAc      >  2:269512/1‑140 (MQ=255)                                                                                                                 aCTATCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCGAAGGTCTCACTGTGTACTTCGCCTTAAAAAGCGGCTCTGCCACATTAACGTCATTAACAGCGGTGAc      >  1:619964/1‑140 (MQ=255)                                                                                                                     tCGCCGCCACCAACGCTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCGAAGGTCTCACTGTGTACTTCGCCTTAAAAAGCGGCTCTGCCACATTAACGTCATTAACAGCGGTGACCGAt  >  1:918201/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                              TACGGTGACTGCATCTTTCCATAACGGCGTAACAATACAGACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTGCTAGCTTTATCGCTGATCCATCGACTATCGCCGCCACCAACACTGATTTAAGTACCTTAAAGGCAACGGTTGAGGATGGCAGTGGTAACCTGATCGAAGGTCTCACTGTGTACTTCGCCTTAAAAAGCGGCTCTGCCACATTAACGTCATTAACAGCGGTGACCGAT  >  NC_000913/2050733‑2050988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑