Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,112,376	T→C	Q229R (CAG→CGG)	rfbB ←	dTDP‑glucose 4,6‑dehydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,112,376	0	T	C	100.0%	257.3 / NA	70	Q229R (CAG→CGG)	rfbB	dTDP‑glucose 4,6‑dehydratase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/33);  total (37/33)

TAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCTGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGACCATAGTTGTTCGAGCAATTAGTCACAATTGTCGGTAAACCAT  >  NC_000913/2112245‑2112508                                                                                                                                    |                                                                                                                                     tAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCt                                                                                                                              <  1:767738/140‑1 (MQ=255)    tGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTg                                                                                                                           >  2:777159/1‑140 (MQ=255)    tGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTg                                                                                                                           >  2:420403/1‑140 (MQ=255)      aGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGcc                                                                                                                         >  1:832836/1‑140 (MQ=255)        cACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCAt                                                                                                                       >  2:65658/1‑140 (MQ=255)           tACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGcc                                                                                                                         <  1:889982/135‑1 (MQ=255)           tACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATaaa                                                                                                                    <  2:832836/140‑1 (MQ=255)           tACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATaaa                                                                                                                    >  1:901930/1‑140 (MQ=255)           tACATCGATGTTCTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGcc                                                                                                                         >  2:889982/1‑135 (MQ=255)             cATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCt                                                                                                                              <  2:931580/128‑1 (MQ=255)             cATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCt                                                                                                                              >  1:931580/1‑128 (MQ=255)             cATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATa                                                                                                                  >  1:761199/1‑140 (MQ=255)               tCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAgg                                                                                                                <  1:440359/140‑1 (MQ=255)                cGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTg                                                                                                                           >  1:944913/1‑128 (MQ=255)                cGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTg                                                                                                                           <  2:944913/128‑1 (MQ=255)                cGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGt                                                                                                               <  1:745661/140‑1 (MQ=255)                 gATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTa                                                                                                              <  1:95337/140‑1 (MQ=255)                 gATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTa                                                                                                              <  2:901930/140‑1 (MQ=255)                     tttttCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGt                                                                                                               <  2:635963/135‑1 (MQ=255)                     tttttCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGt                                                                                                               >  1:635963/1‑135 (MQ=255)                        ttcttttcGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCtt                                                                                                       >  1:623731/1‑140 (MQ=255)                                 ttGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAg                                                                                              <  1:912908/140‑1 (MQ=255)                                  tgtgCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGt                                                                                             <  1:792212/140‑1 (MQ=255)                                    tgCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGc                                                                                           >  1:389484/1‑140 (MQ=255)                                      cccACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCAt                                                                                         <  2:761199/140‑1 (MQ=255)                                             aTGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAAt                                                                                  <  1:173277/140‑1 (MQ=255)                                              tGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGa                                                                                    >  2:465670/1‑137 (MQ=255)                                              tGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGa                                                                                    <  1:465670/137‑1 (MQ=255)                                              tGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATa                                                                                 <  2:368828/140‑1 (MQ=255)                                                ttATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAAc                                                                               >  1:463601/1‑140 (MQ=255)                                                   taAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAg                                                                            <  2:821909/140‑1 (MQ=255)                                                        ttCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGaa                                                                       >  1:519603/1‑140 (MQ=255)                                                          cACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATa                                                                     >  2:419657/1‑140 (MQ=255)                                                          cACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATa                                                                     >  2:320902/1‑140 (MQ=255)                                                          cACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATa                                                                     >  1:564682/1‑140 (MQ=255)                                                                       ttCGGTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGa                                                        <  2:389484/140‑1 (MQ=255)                                                                           gTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATg                                                    >  2:724136/1‑140 (MQ=255)                                                                             tACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGAt                                                  <  2:623731/140‑1 (MQ=255)                                                                               cgacgGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATaa                                                >  1:45554/1‑140 (MQ=255)                                                                                  cgGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGa                                             <  2:519603/140‑1 (MQ=255)                                                                                      atataACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGaa                                                       >  2:545167/1‑126 (MQ=255)                                                                                      atataACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGaa                                                       <  1:545167/126‑1 (MQ=255)                                                                                            cgcacgcGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGACCATAgttgt                                   >  2:510653/1‑140 (MQ=255)                                                                                              cacgcGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGAt                                                  >  1:753361/1‑123 (MQ=255)                                                                                              cacgcGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGAt                                                  <  2:753361/123‑1 (MQ=255)                                                                                              cacgcGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGACCATAGTTGTTc                                 >  2:323397/1‑140 (MQ=255)                                                                                               acgcGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGACCATAGTTGTTCg                                >  2:754172/1‑140 (MQ=255)                                                                                               acgcGCATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGACCATAGTTGTTCg                                >  1:481351/1‑140 (MQ=255)                                                                                                 gcgcATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGACCATAGTTGTTCGAg                              <  1:724136/140‑1 (MQ=255)                                                                                                 gcgcATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGACCATAGTTGTTCGAg                              >  2:579669/1‑140 (MQ=255)                                                                                                 gcgcATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGACCATAGTTGTTCGAg                              <  1:419657/140‑1 (MQ=255)                                                                                                 gcgcATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGACCATAGTTGTTCGAg                              >  1:896959/1‑140 (MQ=255)                                                                                                 acgcATGATCTTCAACATACAACCAGTCGAGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGACCATAGTTGTTCGAg                              >  2:451326/2‑140 (MQ=255)                                                                                                    cATGATCTTCAACATACAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGACCATAGTTGTTCGAGCaa                           <  2:481351/140‑1 (MQ=255)                                                                                                                   aCAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGACCATAGTTGTTCGAGCAATTAGTCACAATTGTc            >  2:654801/1‑140 (MQ=255)                                                                                                                   aCAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGACCATAGTTGTTCGAGCAATTAGTCACAATTGTc            >  2:940626/1‑140 (MQ=255)                                                                                                                   aCAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGACCATAGTTGTTCGAGCAATTAGTCACAATTGTc            >  2:737402/1‑140 (MQ=255)                                                                                                                   aCAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGACCATAGTTGTTCGAGCAATTAGTCACAATTGTc            >  2:528919/1‑140 (MQ=255)                                                                                                                   aCAACCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGACCATAGTTGTTCGAGCAATTAGTCACAATTGTc            >  1:223051/1‑140 (MQ=255)                                                                                                                     aaCCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGACCATAGTTGTTCGAGCaa                           >  2:776734/1‑123 (MQ=255)                                                                                                                     aaCCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGACCATAGTTGTTCGAGCaa                           <  1:776734/123‑1 (MQ=255)                                                                                                                     aaCCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGACCATAGTTGTTCGAGCAATTAGTCACAATTGTCgg          <  2:223051/140‑1 (MQ=255)                                                                                                                      aCCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGACCATAGTTGTTCGAGCAATTAGTCACAATTGTCGGt         <  1:777159/140‑1 (MQ=255)                                                                                                                      aCCAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGACCATAGTTGTTCGAGCAATTAGTCACAATTGTCGGt         <  1:737402/140‑1 (MQ=255)                                                                                                                       ccAGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGACCATAGTTGTTCGAGCAATTAGTCACAATTGTCGGTa        <  1:114389/140‑1 (MQ=255)                                                                                                                         aGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGACCATAGTTGTTCGAGCAATTAGTCACAATTGTCGGTaaa      <  1:528919/140‑1 (MQ=255)                                                                                                                         aGTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGACCATAGTTGTTCGAGCAATTAGTCACAATTGTCGGTaaa      <  1:420403/140‑1 (MQ=255)                                                                                                                          gTCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGACCATAGTTGTTCGAGCAATTAGTCACAATTGTCGGTAAAc     >  1:612486/1‑140 (MQ=255)                                                                                                                           tCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGACCATAGTTGTTCGAGCAATTAGTCACAATTGTCGGTAAAcc    <  1:579669/140‑1 (MQ=255)                                                                                                                           tCGCGGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGACCATAGTTGTTCGAGCAATTAGTCACAATTGTCGGTAAAcc    >  1:139521/1‑140 (MQ=255)                                                                                                                           tCGCGGATCCGACCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGACCATAGTTGTTCGAGCAATTAGTCACAATTGTCGGTAAAcc    >  1:117088/1‑140 (MQ=255)                                                                                                                             gcgGATCCGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTTCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGACCATAGTTGTTCGAGCAATTAGTCACAATTGTCGGTAAACCat  <  2:362317/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                     TAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTCAACATACAACCAGTCGCGGATCTGATCTCCTTTGCCATAAATAGGTAATGCCTTACCTTCCAGTGCATTAAGAATAACCAGTGGAATAAGCTTTTCCGGGAAATGATAAGGACCATAGTTGTTCGAGCAATTAGTCACAATTGTCGGTAAACCAT  >  NC_000913/2112245‑2112508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑