Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,252,094	C→T	A132V (GCC→GTC)	yeiI →	putative sugar kinase YeiI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,252,094	0	C	T	100.0%	174.6 / NA	50	A132V (GCC→GTC)	yeiI	putative sugar kinase YeiI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/26);  total (24/26)

AATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAA  >  NC_000913/2251960‑2252227                                                                                                                                       |                                                                                                                                      aaTTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGTCGGCg                                                                                                                                  >  2:892820/1‑140 (MQ=255)   ttCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGTCGGCGTg                                                                                                                                >  1:194372/1‑140 (MQ=255)   ttCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGTCGGCGTg                                                                                                                                >  1:217676/1‑140 (MQ=255)   ttCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGTCGGCGTg                                                                                                                                >  1:461423/1‑140 (MQ=255)   ttCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGTCGGCGTg                                                                                                                                >  2:118308/1‑140 (MQ=255)    tCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGTCGGCGTGa                                                                                                                               <  2:217676/140‑1 (MQ=255)    tCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGTCGGCGTGa                                                                                                                               <  2:186778/140‑1 (MQ=255)              gcgggcggCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGTCGGCGTGAATGTCTCCgg                                                                                                                     <  2:461423/140‑1 (MQ=255)                            aCGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGc                                                                                                       <  1:926005/140‑1 (MQ=255)                            aCGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGc                                                                                                       <  2:67233/140‑1 (MQ=255)                            aCGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGc                                                                                                       <  1:920100/140‑1 (MQ=255)                            aCGCAACATCGCCCACAATCTGGCGCTGTTAGGCCATGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGc                                                                                                       <  1:730844/140‑1 (MQ=255)                              gCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCAt                                                                                                     <  1:539283/140‑1 (MQ=255)                                aaCATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATgg                                                                                                   >  2:322282/1‑140 (MQ=255)                                      gCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAg                                                                                             <  1:322282/140‑1 (MQ=255)                                       cccACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGTCGGCGTGAATGTCTCCGGCTGCGTTAGTTTGCATGGTCAAAGc                                                                                            >  1:914822/1‑140 (MQ=255)                                       cccACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGTCGGCGTGAATGTCTCCGGCTGAGTTCGTTTGCATGGTCAAAGc                                                                                            >  1:932874/1‑140 (MQ=255)                                              tCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGa                                                                                     <  2:656005/140‑1 (MQ=255)                                               cTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGAc                                                                                    <  1:118308/140‑1 (MQ=255)                                                tGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACg                                                                                   >  1:379825/1‑140 (MQ=255)                                                        ttAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGc                                                                           >  1:833752/1‑140 (MQ=255)                                                                tGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCa                                                                   >  1:64419/1‑140 (MQ=255)                                                                tGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCa                                                                   >  1:155969/1‑140 (MQ=255)                                                                  aCGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAAt                                                                 >  1:723018/1‑140 (MQ=255)                                                                  aCGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAAt                                                                 >  2:45751/1‑140 (MQ=255)                                                                  aCGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGTCGGCGTGAATGTCCCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAAt                                                                 >  1:141740/1‑140 (MQ=255)                                                                             cTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCa                                                      <  2:194372/140‑1 (MQ=255)                                                                                    tGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTg                                               <  2:833752/140‑1 (MQ=255)                                                                                      aTTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCt                                             <  2:723018/140‑1 (MQ=255)                                                                                       ttGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTg                                            >  1:468749/1‑140 (MQ=255)                                                                                          gCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCg                                         >  2:770863/1‑140 (MQ=255)                                                                                                aCTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAAc                                   >  1:438474/1‑140 (MQ=255)                                                                                                        ggCGAAATGCTCCTGGAAGAAACGCGCCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCa                           <  2:855731/140‑1 (MQ=255)                                                                                                        ggCGAAATGCTCCTGGAAGAAACGCGCCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCa                           <  2:64419/140‑1 (MQ=255)                                                                                                          cGAAATGCTCCTGGAAGAAACGCGCCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATc                         >  2:476343/1‑140 (MQ=255)                                                                                                            aaaTGCTCCTGGAAGAAACGCGCCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTGTCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATctg                       >  2:831346/1‑140 (MQ=255)                                                                                                             aaTGCTCCTGGAAGAAACGCGCCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATctgc                      <  1:228716/140‑1 (MQ=255)                                                                                                              aTGCTCCTGGAAGAAACGCGCCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATctgct                     >  1:205958/1‑140 (MQ=255)                                                                                                               tGCTCCTGGAAGAAACGCGCCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATctgctg                    <  2:205958/140‑1 (MQ=255)                                                                                                                 cTCCTGGAAGAAACGCGCCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCa                           >  2:688583/1‑131 (MQ=255)                                                                                                                 cTCCTGGAAGAAACGCGCCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCa                           <  1:688583/131‑1 (MQ=255)                                                                                                                  tCCTGGAAGAAACGCGCCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGaa                 <  2:389976/140‑1 (MQ=255)                                                                                                                      ggAAGAAACGCGCCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGt             >  1:461885/1‑140 (MQ=255)                                                                                                                       gaagaaACGCGCCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGtt            <  1:476343/140‑1 (MQ=255)                                                                                                                         agaaACGCGCCGTGTCGGCGTGAATGTCCCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGa          <  2:141740/140‑1 (MQ=255)                                                                                                                          gaaACGCGCCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGac         <  2:455959/140‑1 (MQ=255)                                                                                                                          gaaACGCGCCGCGTCGGCGTGAATGTCTCCGGCTGCGTGCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGac         <  2:932874/140‑1 (MQ=255)                                                                                                                              cgcgCCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCg     >  2:256060/1‑140 (MQ=255)                                                                                                                              cgcgCCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCg     >  1:764229/1‑140 (MQ=255)                                                                                                                               gcgcCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGc    <  2:627069/140‑1 (MQ=255)                                                                                                                               gcgcCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGc    <  2:379825/140‑1 (MQ=255)                                                                                                                                 gcCGTGTCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCaa  <  1:770863/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      AATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAGTTGACACCGCAA  >  NC_000913/2251960‑2252227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑