Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,357,149	A→G	E82E (GAA→GAG)	rpnE →	inactive recombination‑promoting nuclease‑like protein RpnE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,357,149	0	A	G	100.0%	148.2 / NA	41	E82E (GAA→GAG)	rpnE	inactive recombination‑promoting nuclease‑like protein RpnE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/13);  total (28/13)

CTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCTGGTGGTGCCGTTGCTGTTTTATCATGGCGAA  >  NC_000913/2357018‑2357281                                                                                                                                    |                                                                                                                                     cTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAg                                                                                                                              <  1:871561/140‑1 (MQ=255)   ttGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGct                                                                                                                            <  1:454566/140‑1 (MQ=255)      cAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTg                                                                                                                         <  1:563443/140‑1 (MQ=255)           tGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGaa                                                                                                                    <  2:623888/140‑1 (MQ=255)                            gAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGGGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAatat                                                                                                            >  2:659935/1‑131 (MQ=255)                            gAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAatat                                                                                                            <  1:659935/131‑1 (MQ=255)                            gAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCg                                                                                                   >  2:121666/1‑140 (MQ=255)                                 cACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTaa                                                                                              <  1:522211/140‑1 (MQ=255)                                  aCTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAAt                                                                                             >  1:946137/1‑140 (MQ=255)                                   cTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATg                                                                                            >  1:304740/1‑140 (MQ=255)                                   cTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATg                                                                                            >  1:766808/1‑140 (MQ=255)                                              gAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCAc                                                                                 <  2:766808/140‑1 (MQ=255)                                                      tttACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCa                                                                         >  2:772328/1‑140 (MQ=255)                                                              cTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGt                                                                 <  2:437907/140‑1 (MQ=255)                                                                 actactCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCAc                                                              <  2:411345/140‑1 (MQ=255)                                                                  ctactCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCAcc                                                             <  2:78686/140‑1 (MQ=255)                                                                           tGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAg                                                    >  1:253914/1‑140 (MQ=255)                                                                                    gTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACa                                           >  2:294/1‑140 (MQ=255)                                                                                         tGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACa                                                       >  2:162292/1‑123 (MQ=255)                                                                                         tGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACa                                                       <  1:162292/123‑1 (MQ=255)                                                                                          ggAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTcc                                     >  2:142177/1‑140 (MQ=255)                                                                                            aaaCCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCg                                   <  2:253914/140‑1 (MQ=255)                                                                                                 aGCGACGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCtggt                              >  2:66671/1‑140 (MQ=255)                                                                                                      cGGTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCTGGTGGTGc                         >  1:687145/1‑140 (MQ=255)                                                                                                       ggTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCTGGTGGTGcc                        >  1:243542/1‑140 (MQ=255)                                                                                                        gTGACGGCTATATCTACTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCTGGTGGTGCCg                       <  2:304740/140‑1 (MQ=255)                                                                                                                        cTGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCTGGTGGTGCCGTTGCTGTTTTATCATg       >  1:321/1‑140 (MQ=255)                                                                                                                         tGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCTGGTGGTGCCGTTGCTGTTTTATCATgg      >  2:158482/1‑140 (MQ=255)                                                                                                                         tGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCTGGTGGTGCCGTTGCTGTTTTATCATgg      >  1:365071/1‑140 (MQ=255)                                                                                                                         tGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCTGGTGGTGCCGTTGCTGTTTTATCATgg      >  1:358664/1‑140 (MQ=255)                                                                                                                         tGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCTGGTGGTGCCGTTGCTGTTTTATCATgg      >  2:747064/1‑140 (MQ=255)                                                                                                                         tGCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCTGGTGGTGCCGTTGCTGTTTTATCATgg      >  1:288123/1‑140 (MQ=255)                                                                                                                          gCGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCTGGTGGTGCCGTTGCTGTTTTATCATGGc     >  1:701397/1‑140 (MQ=255)                                                                                                                           cGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCTGGTGGTGCCGTTGCTGTTTTATCATGGCg    >  1:479712/1‑140 (MQ=255)                                                                                                                           cGTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTCCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCTGGTGGTGCCGTTGCTGTTTTATCATGGCg    >  1:346126/1‑140 (MQ=255)                                                                                                                            gTGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCTGGTGGTGCCGTTGCTGTTTTATCATGGCGa   >  2:415235/1‑140 (MQ=255)                                                                                                                             tGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCTGGTGGTGCCGTTGCTGTTTTATCATGGCGaa  >  1:884674/1‑140 (MQ=255)                                                                                                                             tGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCTGGTGGTGCCGTTGCTGTTTTATCATGGCGaa  >  1:562630/1‑140 (MQ=255)                                                                                                                             tGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCTGGTGGTGCCGTTGCTGTTTTATCATGGCGaa  >  1:505609/1‑140 (MQ=255)                                                                                                                             tGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCTGGTGGTGCCGTTGCTGTTTTATCATGGCGaa  >  2:506607/1‑140 (MQ=255)                                                                                                                             tGATTGAGCATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGACCCGCTGGTGGTGCCGTTGCTGTTTTATCATGGCGaa  >  2:420400/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                     CTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAGTCCCGCTGGTGGTGCCGTTGCTGTTTTATCATGGCGAA  >  NC_000913/2357018‑2357281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑