Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,527,823	T→C	V293A (GTG→GCG)	yfeH →	putative solute:Na(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,527,823	0	T	C	100.0%	154.3 / NA	43	V293A (GTG→GCG)	yfeH	putative solute:Na(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/25);  total (18/25)

GCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTC  >  NC_000913/2527696‑2527956                                                                                                                                |                                                                                                                                      gCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCtgat                                                                                                                           >  1:28151/1‑140 (MQ=255)    cGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTgatgat                                                                                                                        >  1:104853/1‑140 (MQ=255)     gCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCtgat                                                                                                                           <  1:197132/136‑1 (MQ=255)     gCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCtgat                                                                                                                           >  2:197132/1‑136 (MQ=255)     gCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGAtt                                                                                                                       <  1:661733/140‑1 (MQ=255)      ccGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGAttt                                                                                                                      >  1:719651/1‑140 (MQ=255)           tGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCAt                                                                                                                 <  2:28151/140‑1 (MQ=255)            gAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATc                                                                                                                >  1:712996/1‑140 (MQ=255)             aGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCa                                                                                                               <  1:415149/140‑1 (MQ=255)               cTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGa                                                                                                             <  1:755623/140‑1 (MQ=255)                  cAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATcc                                                                                                          <  2:104853/140‑1 (MQ=255)                       aGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTg                                                                                                     <  1:265586/140‑1 (MQ=255)                            gATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGt                                                                                                <  2:106405/140‑1 (MQ=255)                             aTGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTc                                                                                               >  2:503893/1‑140 (MQ=255)                             aTGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTc                                                                                               >  1:214179/1‑140 (MQ=255)                                 aaTTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCtgtg                                                                                           <  2:558073/140‑1 (MQ=255)                                 aaTTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCtgtg                                                                                           <  2:214179/140‑1 (MQ=255)                                       tATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGc                                                                                     >  2:371537/1‑140 (MQ=255)                                       tATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGc                                                                                     >  1:639757/1‑140 (MQ=255)                                          cGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTgg                                                                                  <  2:521952/140‑1 (MQ=255)                                           gTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGgc                                                                                 <  2:719651/140‑1 (MQ=255)                                           gTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGgc                                                                                 >  2:228054/1‑140 (MQ=255)                                                ttGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTc                                                                            <  1:300374/140‑1 (MQ=255)                                                 tgtgGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCg                                                                           >  2:568143/1‑140 (MQ=255)                                                   tgGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCtgtg                                                                                           <  1:546399/122‑1 (MQ=255)                                                   tgGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCtgtg                                                                                           >  2:546399/1‑122 (MQ=255)                                                      ttCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACa                                                                      <  1:345447/140‑1 (MQ=255)                                                        cGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACaaa                                                                    >  1:948083/1‑140 (MQ=255)                                                          aaaaaGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACg                                                                  <  2:639757/140‑1 (MQ=255)                                                                agTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGAc                                                            <  2:712996/140‑1 (MQ=255)                                                                 gTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGAcc                                                           <  1:503893/140‑1 (MQ=255)                                                                    tGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGaa                                                        <  1:371537/140‑1 (MQ=255)                                                                                 cccGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGgcgc                                           <  1:253919/140‑1 (MQ=255)                                                                                      tGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGcagcag                                      >  2:477284/1‑140 (MQ=255)                                                                                                 cTGTTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAgcgc                           <  1:172743/140‑1 (MQ=255)                                                                                                      ccccACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATa                      <  2:948083/140‑1 (MQ=255)                                                                                                              cGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTaa              >  2:303822/1‑140 (MQ=255)                                                                                                              cGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTaa              >  1:375007/1‑140 (MQ=255)                                                                                                                  gATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCg          <  1:477284/140‑1 (MQ=255)                                                                                                                   aTCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCgg         <  1:568143/140‑1 (MQ=255)                                                                                                                   aTCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCgg         >  1:316614/1‑140 (MQ=255)                                                                                                                      ggTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACg      >  1:196445/1‑140 (MQ=255)                                                                                                                          tgatgGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTc  <  1:303822/140‑1 (MQ=255)                                                                                                                                |                                                                                                                                      GCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTC  >  NC_000913/2527696‑2527956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑