Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,641,940	G→A	A302V (GCC→GTC)	rodZ ←	transmembrane component of cytoskeleton

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,641,940	0	G	A	100.0%	88.9 / NA	26	A302V (GCC→GTC)	rodZ	transmembrane component of cytoskeleton
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/12);  total (14/12)

TACTGCGCCGGTGATTGTTCGGCATTGAGGGTCAGACGCGCAACCTGGTTAGTTCTGATAAAACGACTCAGATCGACAGGTTTCCCTTGATACTGGATCTGTACTGCGGCTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGTT  >  NC_000913/2641832‑2642073                                                                                                             |                                                                                                                                      tACTGCGCCGGTGATTGTTCGGCATTGAGGGTCAGACGCGCAACCTGGTTAGTTCTGATAAAACGACTCAGATCGACAGGTTTCCCTTGATACTGGATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGc                                                                                                        <  1:719388/140‑1 (MQ=255) tACTGCGCCGGTGATTGTTCGGCATTGAGGGTCAGACGCGCAACCTGGTTAGTTCTGATAAAACGACTCAGATCGACAGGTTTCCCTTGATACTGGATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGc                                                                                                        <  1:794252/140‑1 (MQ=255)        ccGGTGATTGTTCGGCATTGAGGGTCAGACGCGCAACCTGGTTAGTTCTGATAAAACGACTCAGATCGACAGGTTTCCCTTGATACTGGATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCg                                                                                                 >  2:312490/1‑140 (MQ=255)         cGGTGATTGTTCGGCATTGAGGGTCAGACGCGCAACCTGGTTAGTTCTGATAAAACGACTCAGATCGACAGGTTTCCCTTGATACTGGATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCgg                                                                                                <  1:694876/140‑1 (MQ=255)            tGATTGTTCGGCATTGAGGGTCAGACGCGCAACCTGGTTAGTTCTGATAAAACGACTCAGATCGACAGGTTTCCCTTGATACTGGATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGATa                                                                                             >  2:414579/1‑140 (MQ=255)                     ggCATTGAGGGTCAGACGCGCAACCTGGTTAGTTCTGATAAAACGACTCAGATCGACAGGTTTCCCTTGATACTGGATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAAt                                                                                    >  1:715086/1‑140 (MQ=255)                        aTTGAGGGTCAGACGCGCAACCTGGTTAGTTCTGATAAAACGACTCAGATCGACAGGTTTCCCTTGATACTGGATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTAc                                                                                 <  2:715086/140‑1 (MQ=255)                               gTCAGACGCGCAACCTGGTTAGTTCTGATAAAACGACTCAGATCGACAGGTTTCCCTTGATACTGGATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCttt                                                                          <  2:514015/140‑1 (MQ=255)                                         cAACCTGGTTAGTTCTGATAAAACGACTCAGATCGACAGGTTTCCCTTGATACTGGATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACg                                                                       <  1:394082/133‑1 (MQ=255)                                         cAACCTGGTTAGTTCTGATAAAACGACTCAGATCGACAGGTTTCCCTTGATACTGGATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACg                                                                       >  2:394082/1‑133 (MQ=255)                                                      tCTGATAAAACGACTCAGATCGACAGGTTTCCCTTGATACTGGATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAAtt                                                   >  1:563832/1‑140 (MQ=255)                                                         gATAAAACGACTCAGATCGACAGGTTTCCCTTGATACTGGATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTaaa                                                        <  1:757189/132‑1 (MQ=255)                                                         gATAAAACGACTCAGATCGACAGGTTTCCCTTGATACTGGATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTaaa                                                        >  2:453991/1‑132 (MQ=255)                                                         gATAAAACGACTCAGATCGACAGGTTTCCCTTGATACTGGATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTaaa                                                        >  2:757189/1‑132 (MQ=255)                                                         gATAAAACGACTCAGATCGACAGGTTTCCCTTGATACTGGATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTaaa                                                        <  1:453991/132‑1 (MQ=255)                                                                        aTCGACAGGTTTCCCTTGATACTGGATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAg                                 >  2:579162/1‑140 (MQ=255)                                                                                  ttCCCTTGATACTGGATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTccag                       >  1:676946/1‑140 (MQ=255)                                                                                          aTACTGGATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAAt               >  1:408577/1‑140 (MQ=255)                                                                                              tGGATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGc           >  2:267046/1‑140 (MQ=255)                                                                                                gATCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAg         >  1:12307/1‑140 (MQ=255)                                                                                                  tCTGTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTa       <  2:12307/140‑1 (MQ=255)                                                                                                     gTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTGCCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAg    >  2:884470/1‑140 (MQ=255)                                                                                                     gTACTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAg    >  2:780081/1‑140 (MQ=255)                                                                                                       aCTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGtt  <  1:780081/140‑1 (MQ=255)                                                                                                       aCTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGtt  <  1:579162/140‑1 (MQ=255)                                                                                                       aCTGCGACTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGtt  <  2:88081/140‑1 (MQ=255)                                                                                                             |                                                                                                                                      TACTGCGCCGGTGATTGTTCGGCATTGAGGGTCAGACGCGCAACCTGGTTAGTTCTGATAAAACGACTCAGATCGACAGGTTTCCCTTGATACTGGATCTGTACTGCGGCTGGCGCACCAATTTTCAGTTTGTACGGTGCCTGGCCGGTTAAGTTCAAATTACCGTCTTTACGCTGCATACCGCTAAACAATTTTTTACCGGTAGCATCAGTGACCTCCAGCCAGCAATCGGCAGTAAAGTT  >  NC_000913/2641832‑2642073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑