Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,674,415	G→A	Q92* (CAA→TAA)	yphB ←	putative aldose 1‑epimerase YphB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,674,415	0	G	A	100.0%	295.6 / NA	85	Q92* (CAA→TAA)	yphB	putative aldose 1‑epimerase YphB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (43/42);  total (43/42)

CTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTCACGCCCCTGCCAGACAAAACGATTGCCGCTCACCCGGTTTGCAAATGGCACCAGCGGAAAACA  >  NC_000913/2674280‑2674550                                                                                                                                        |                                                                                                                                        cTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCAc                                                                                                                                     >  1:250058/1‑140 (MQ=255)   tGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTc                                                                                                                                   >  1:359263/1‑140 (MQ=255)   tGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTc                                                                                                                                   >  2:889473/1‑140 (MQ=255)   tGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTc                                                                                                                                   >  2:332576/1‑140 (MQ=255)   tGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTc                                                                                                                                   >  2:20487/1‑140 (MQ=255)    gATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCGCTCg                                                                                                                                  >  1:606013/1‑140 (MQ=255)     aTTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGc                                                                                                                                 <  1:810428/140‑1 (MQ=255)      ttGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGcc                                                                                                                                >  2:28816/1‑140 (MQ=255)        ggTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCcca                                                                                                                              >  2:153760/1‑140 (MQ=255)         gTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCa                                                                                                                                      <  2:603498/131‑1 (MQ=255)         gTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCa                                                                                                                                      >  1:603498/1‑131 (MQ=255)              agagagCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCcagcaagc                                                                                                                        <  1:153760/140‑1 (MQ=255)               gagagCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCagccagcc                                                                                                                       >  1:852739/1‑140 (MQ=255)               gagagCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCagccagcc                                                                                                                       >  1:754099/1‑140 (MQ=255)                  ggtctccccGTCGGCTTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATc                                                                                                                    <  2:884566/133‑1 (MQ=255)                  agCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATc                                                                                                                    <  2:29917/140‑1 (MQ=255)                  agCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATc                                                                                                                    <  1:521882/140‑1 (MQ=255)                    cGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGc                                                                                                                  <  2:852739/140‑1 (MQ=255)                    cGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGc                                                                                                                  <  1:332576/140‑1 (MQ=255)                       cACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCgt                                                                                                               >  2:930253/1‑140 (MQ=255)                              aGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATa                                                                                                        <  2:359263/140‑1 (MQ=255)                              aGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATa                                                                                                        <  2:754099/140‑1 (MQ=255)                              aGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATa                                                                                                        >  1:853608/1‑140 (MQ=255)                                ggTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGt                                                                                                      <  2:218792/139‑1 (MQ=255)                                cGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGt                                                                                                      <  1:493967/140‑1 (MQ=255)                                cGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGt                                                                                                      <  2:22910/140‑1 (MQ=255)                                   aTCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTgcg                                                                                                   <  1:446755/140‑1 (MQ=255)                                    tCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTgcgc                                                                                                  >  2:537205/1‑140 (MQ=255)                                       gccgTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATc                                                                                               >  2:55362/1‑140 (MQ=255)                                          gTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCa                                                                                            >  2:610732/1‑140 (MQ=255)                                           ttAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCAc                                                                                           <  2:393918/140‑1 (MQ=255)                                            tAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACt                                                                                          <  1:55362/140‑1 (MQ=255)                                             aaaTGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTc                                                                                         >  1:215722/1‑140 (MQ=255)                                                 gAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACa                                                                                     <  2:511702/140‑1 (MQ=255)                                                   aaCGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACAtt                                                                                   <  2:113590/140‑1 (MQ=255)                                                     cGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACAtt                                                                                   <  2:779396/138‑1 (MQ=255)                                                     cGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACAtt                                                                                   >  1:779396/1‑138 (MQ=255)                                                     cGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCg                                                                                 >  1:204689/1‑140 (MQ=255)                                                      gCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCgg                                                                                <  1:269446/140‑1 (MQ=255)                                                        cTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACt                                                                                          >  2:331080/1‑128 (MQ=255)                                                        cTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACt                                                                                          <  1:331080/128‑1 (MQ=255)                                                         tGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTg                                                                             <  2:204689/140‑1 (MQ=255)                                                          gACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGc                                                                            <  1:259100/140‑1 (MQ=255)                                                           aCTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCa                                                                           >  1:141794/1‑140 (MQ=255)                                                                 ccGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGAt                                                                     >  2:335477/1‑140 (MQ=255)                                                                   gATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGt                                                                               >  2:768951/1‑128 (MQ=255)                                                                   gATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGt                                                                               <  1:768951/128‑1 (MQ=255)                                                                     tAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTc                                                                 >  1:468381/1‑140 (MQ=255)                                                                          aTAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTCACGcc                                                            >  2:702324/1‑140 (MQ=255)                                                                               cacCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTg                                                                       <  1:452309/124‑1 (MQ=255)                                                                               cacCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTg                                                                       >  2:452309/1‑124 (MQ=255)                                                                                 ccGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTCACGCCCCTGCCa                                                     <  1:537205/140‑1 (MQ=255)                                                                                  cGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTCACGCCCCTg                                                        >  1:136811/1‑136 (MQ=255)                                                                                  cGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTCACGCCCCTg                                                        <  2:136811/136‑1 (MQ=255)                                                                                  cGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTCACGCCCCTGc                                                       <  1:382747/137‑1 (MQ=255)                                                                                  cGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTCACGCCCCTGc                                                       >  2:382747/1‑137 (MQ=255)                                                                                  cGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTCACGCCCCTGCCAg                                                    >  1:929223/1‑140 (MQ=255)                                                                                  cGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTCACGCCCCTGCCAg                                                    >  2:577169/1‑140 (MQ=255)                                                                                  cGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTCACGCCCCTGCCAg                                                    >  1:195014/1‑140 (MQ=255)                                                                                    cTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTCACGCCCCTGCCAGAc                                                  >  1:252780/1‑140 (MQ=255)                                                                                            ttCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTCACGCCCCTGCCAGACAAAACGAt                                          <  2:853608/140‑1 (MQ=255)                                                                                            ttCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTCACGCCCCTGCCAGACAAAACGAt                                          >  1:904631/1‑140 (MQ=255)                                                                                            ttCATACACCAGACACAAACCATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTCACGCCCCTGCCAGACAAAACGAt                                          <  1:786394/140‑1 (MQ=255)                                                                                             tCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTCACGCCCCTGCCAGACAAAACGAtt                                         <  1:889473/140‑1 (MQ=255)                                                                                                 acacCAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTCACGCCCCTGCCAGACAAAACGATTGCCg                                     >  1:802457/1‑140 (MQ=255)                                                                                                    ccAGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGTCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTCACGCCCCTGCCAGACAAAACGATTGCCGCTc                                  >  2:656824/1‑140 (MQ=255)                                                                                                      aGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTCACGCCCCTGCCAGACAAAACGATTGCCGCTCAc                                >  2:232059/1‑140 (MQ=255)                                                                                                      aGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTCACGCCCCTGCCAGACAAAACGATTGCCGCTCAc                                <  2:215722/140‑1 (MQ=255)                                                                                                      aGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTCACGCCCCTGCCAGACAAAACGATTGCCGCTCAc                                >  1:410067/1‑140 (MQ=255)                                                                                                      aGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTCACGCCCCTGCCAGACAAAACGATTGCCGCTCAc                                <  2:252780/140‑1 (MQ=255)                                                                                                      aGACACAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTCACGCCCCTGCCAGACAAAACGATTGCCGCTCAc                                >  1:166142/1‑140 (MQ=255)                                                                                                        acacAAACTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTCACGCCCCTGCCAGACAAAACGATTGCCGCTCAccc                              >  2:548553/1‑140 (MQ=255)                                                                                                             aaCTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTCACGCCCCTGCCAGACAAAACGATTGCCGCTCACCCGGttt                         >  1:553185/1‑140 (MQ=255)                                                                                                             aaCTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTCACGCCCCTGCCAGACAAAACGATTGCCGCTCACCCGGttt                         <  2:298963/140‑1 (MQ=255)                                                                                                              aCTATCATCGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTCACGCCCCTGCCAGACAAAACGATTGCCGCTCACCCGGTTTg                        <  1:930253/140‑1 (MQ=255)                                                                                                                   catcGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTCACGCCCCTGCCAGACAAAACGATTGCCGCTCACCCGGTTTGCAAAt                   <  1:335477/140‑1 (MQ=255)                                                                                                                      cGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGGTACTCACGCCCCTGCCAGACAAAACGATTGCCGCTCACCCGGTTTGCAAATGGc                >  2:263209/1‑140 (MQ=255)                                                                                                                      cGCTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGGTACTCACGCCCCTGCCAGACAAAACGATTGCCGCTCACCCGGTTTGCAAATGGc                >  2:263207/1‑140 (MQ=255)                                                                                                                        cTGTGCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTCACGCCCCTGCCAGACAAAACGATTGCCGCTCACCCGGTTTGCAAATGGCAc              <  2:802457/140‑1 (MQ=255)                                                                                                                         tgtgCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTCACGCCCCTGCCAGACAAAACGATTGCCGCTCACCCGGTTTGCAAATGGCAcc             <  2:904631/140‑1 (MQ=255)                                                                                                                           tgCGAGACACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTCACGCCCCTGCCAGACAAAACGATTGCCGCTCACCCGGTTTGCAAATGGCACCAg           >  1:231704/1‑140 (MQ=255)                                                                                                                               agaCACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTCACGCCCCTGCCAGACAAAACGATTGCCGCTCACCCGGTTTGCAAATGGCACCAGCGGa       <  2:250058/140‑1 (MQ=255)                                                                                                                               agaCACATTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTCACGCCCCTGCCAGACAAAACGATTGCCGCTCACCCGGTTTGCAAATGGCACCAGCGGa       <  2:166142/140‑1 (MQ=255)                                                                                                                                    caTTACCGCTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTCACGCCCCTGCCAGACAAAACGATTGCCGCTCACCCGGTTTGCAAATGGCACCAGCGGAAAACa  <  2:606013/140‑1 (MQ=255)                                                                                                                                     aTTACCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTCACGCCCCTGCCAGACAAAACGATTGCCGCTCACCCGGTTTGCAAATGGCAc              <  1:421859/127‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        CTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTCAACATTCGGTTGCAGTTGATACTCACGCCCCTGCCAGACAAAACGATTGCCGCTCACCCGGTTTGCAAATGGCACCAGCGGAAAACA  >  NC_000913/2674280‑2674550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑