Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,741,761	A→G	intergenic (+36/‑114)	yfiL → / → yfiR	DUF2799 domain‑containing lipoprotein YfiL/DUF4154 domain‑containing protein YfiR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,741,761	0	A	G	100.0%	115.9 / NA	33	intergenic (+36/‑114)	yfiL/yfiR	DUF2799 domain‑containing lipoprotein YfiL/DUF4154 domain‑containing protein YfiR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/17);  total (16/17)

CTTTCCTGCCAGCTGCAACAATGTTGAAAATGCCAGCCAACTGCATGAAGTATGGCAAAAAGGGGCTGATGAAAATGCCAGCACAATACGATTAAATTAATTAAAACCCCACAAATAAATTAAACATAAGATTTTACTCATTGTGTTTTATTTCTCACATTGATGACGGTCGCATGAAAAAATGATATTTTAATGAGCATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGA  >  NC_000913/2741626‑2741892                                                                                                                                        |                                                                                                                                    cTTTCCTGCCAGCTGCAACAATGTTGAAAATGCCAGCCAACTGCATGAAGTATGGCAAAAAGGGGCTGATGAAAATGCCAGCACAATACGATTAAATTAATTAAAACCCCACAAATAAATTAAACATAAGATTTTGCTCa                                                                                                                                 <  1:569173/140‑1 (MQ=255)   ttCCTGCCAGCTGCAACAATGTTGAAAATGCCAGCCAACTGCATGAAGTATGGCAAAAAGGGGCTGATGAAAATGCCAGCACAATACGATTAAATTAATTAAAACCCCACAAATAAATTAAACATAAGATTTTGCTCAtt                                                                                                                               <  2:567431/140‑1 (MQ=255)     ccTGCCAGCTGCAACAATGTTGAAAATGCCAGCCAACTGCATGAAGTATGGCAAAAAGGGGCTGATGAAAATGCCAGCACAATACGATTAAATTAATTAAAACCCCACAAATAAATTAAACATAAGATTTTGCTCATtgt                                                                                                                             >  1:101526/1‑140 (MQ=255)          cAGCTGCAACAATGTTGAAAATGCCAGCCAACTGCATGAAGTATGGCAAAAAGGGGCTGATGAAAATGCCAGCACAATACGATTAAATTAATTAAAACCCCACAAATAAATTAAACATAAGATTTTGCTCATTGTGtttt                                                                                                                        <  1:772444/140‑1 (MQ=255)             cTGCAACAATGTTGAAAATGCCAGCCAACTGCATGAAGTATGGCAAAAAGGGGCTGATGAAAATGCCAGCACAATACGATTAAATTAATTAAAACCCCACAAATAAATTAAACATAAGATTTTGCTCATTGTGTTTTAtt                                                                                                                     >  2:786672/1‑140 (MQ=255)                    aaTGTTGAAAATGCCAGCCAACTGCATGAAGTATGGCAAAAAGGGGCTGATGAAAATGCCAGCACAATACGATTAAATTAATTAAAACCCCACAAATAAATTAAACATAAGATTTTGCTCATTGTGTTTTATTTCTcaca                                                                                                              <  1:786672/140‑1 (MQ=255)                      tGTTGAAAATGCCAGCCAACTGCATGAAGTATGGCAAAAAGGGGCTGATGAAAATGCCAGCACAATACGATTAAATTAATTAAAACCCCACAAATAAATTAAACATAAGATTTTGCTCATTGTGTTTTATTTCTcaca                                                                                                              <  1:426290/138‑1 (MQ=255)                      tGTTGAAAATGCCAGCCAACTGCATGAAGTATGGCAAAAAGGGGCTGATGAAAATGCCAGCACAATACGATTAAATTAATTAAAACCCCACAAATAAATTAAACATAAGATTTTGCTCATTGTGTTTTATTTCTcaca                                                                                                              >  2:426290/1‑138 (MQ=255)                           aaaaTGCCAGCCAACTGCATGAAGTATGGCAAAAAGGGGCTGATGAAAATGCCAGCACAATACGATTAAATTAATTAAAACCCCACAAATAAATTAAACATAAGATTTTGCTCATTGTGTTTTATTTCTCACATtgatga                                                                                                       >  1:593920/1‑140 (MQ=255)                                  cagccaACTGCATGAAGTATGGCAAAAAGGGGCTGATGAAAATGCCAGCACAATACGATTAAATTAATTAAAACCCCACAAATAAATTAAACATAAGATTTTGCTCATTGTGTTTTATTTCTcaca                                                                                                              >  2:612789/1‑126 (MQ=255)                                  cagccaACTGCATGAAGTATGGCAAAAAGGGGCTGATGAAAATGCCAGCACAATACGATTAAATTAATTAAAACCCCACAAATAAATTAAACATAAGATTTTGCTCATTGTGTTTTATTTCTcaca                                                                                                              <  1:612789/126‑1 (MQ=255)                                        aCTGCATGAAGTATGGCAAAAAGGGGCTGATGAAAATGCCAGCACAATACGATTAAATTAATTAAAACCCCACAAATAAATTAAACATAAGATTTTGCTCATTGTGTTTTATTTCTCACATTGATGACGGTCGCATGaaa                                                                                          <  2:657913/140‑1 (MQ=255)                                           gCATGAAGTATGGCAAAAAGGGGCTGATGAAAATGCCAGCACAATACGATTAAATTAATTAAAACCCCACAAATAAATTAAACATAAGATTTTGCTCATTGTGTTTTATTTCTCACATTGATGACGGTCGCATGaaaaaa                                                                                       >  1:390018/1‑140 (MQ=255)                                                aaGTATGGCAAAAAGGGGCTGATGAAAATGCCAGCACAATACGATTAAATTAATTAAAACCCCACAAATAAATTAAACATAAGATTTTGCTCATTGTGTTTTATTTCTCACATTGATGACGGTCGCATGAAAAAATGata                                                                                  <  1:589128/140‑1 (MQ=255)                                                    aTGGCAAAAAGGGGCTGATGAAAATGCCAGCACAATACTATTAAATTAATTAAAACCCCACAAATAAATTAAACATAAGATTTTGCTCATTGTGTTTTATTTCTCACATTGATGACGGTCGCATGAAAAAATGATAtttt                                                                              <  2:361561/140‑1 (MQ=255)                                                    aTGGCAAAAAGGGGCTGATGAAAATGCCAGCACAATACGATTAAATTAATTAAAACCCCACAAATAAATTAAACATAAGATTTTGCTCATTGTGTTTTATTTCTCACATTGATGACGGTCGCATGAAAAAATGATAtttt                                                                              >  2:283364/1‑140 (MQ=255)                                                           aaaGGGGCTGATGAAAATGCCAGCACAATACGATTAAATTAATTAAAACCCCACAAATAAATTAAACATAAGATTTTGCTCATTGTGTTTTATTTCTCACATTGATGACGGTCGCATGAAAAAATGATATTTTAATGAGc                                                                       >  2:113202/1‑140 (MQ=255)                                                           aaaGGGGCTGATGAAAATGCCAGCACAATACGATTAAATTAATTAAAACCCCACAAATAAATTAAACATAAGATTTTGCTCATTGTGTTTTATTTCTCACATTGATGACGGTCGCATGAAAAAATGATATTTTAATGAGc                                                                       >  1:279272/1‑140 (MQ=255)                                                            aaGGGGCTGATGAAAATGCCAGCACAATACGATTAAATTAATTAAAACCCCACAAATAAATTAAACATAAGATTTTGCTCATTGTGTTTTATTTCTCACATTGATGACGGTCGCATGAAAAAATGATATTTTAATGAGCa                                                                      >  1:613815/1‑140 (MQ=255)                                                                             gCCAGCACAATACGATTAAATTAATTAAAACCCCACAAATAAATTAAACATAAGATTTTGCTCATTGTGTTTTATTTCTCACATTGATGACGGTCGCATGAAAAAATGATATTTTAATGAGCATTCTGAAAAAATATCGa                                                     <  2:390018/140‑1 (MQ=255)                                                                                 gCACAATACGATTAAATTAATTAAAACCCCACAAATAAATTAAACATAAGATTTTGCTCATTGTGTTTTATTTCTCACATTGATGACGGTCGCATGAAAAAATGATATTTTAATGAGCATTCTGAAAAAATATCGATGCa                                                 >  2:107702/1‑140 (MQ=255)                                                                                       tACGATTAAATTAATTAAAACCCCACAAATAAATTAAACATAAGATTTTGCTCATTGTGTTTTATTTCTCACATTGATGACGGTCGCATGAAAAAATGATATTTTAATGAGCATTCTGAAAAAATATCGATGCATTTCGa                                           >  2:234038/1‑140 (MQ=255)                                                                                            ttaaattaaTTAAAACCCCACAAATAAATTAAACATAAGATTTTGCTCATTGTGTTTTATTTCTCACATTGATGACGGTCGCATGAAAAAATGATATTTTAATGAGCATTCTGAAAAAATATCGATGCATTTCGAGCGaa                                      <  1:283364/140‑1 (MQ=255)                                                                                            ttaaattaaTTAAAACCCCACAAATAAATTAAACATAAGATTTTGCTCATTGTGTTTTATTTCTCACATTGATGACGGTCGCATGAAAAAATGATATTTTAATGAGCATTCTGAAAAAATATCGATGCATTTCGAGCGaa                                      >  1:446918/1‑140 (MQ=255)                                                                                             taaattaaTTAAAACCCCACAAATAAATTAAACATAAGATTTTGCTCATTGTGTTTTATTTCTCACATTGATGACGGTCGCATGAAAAAATGATATTTTAATGAGCATTCTGAAAAAATATCGATGCATTTCGAGCGAAg                                     <  2:279272/140‑1 (MQ=255)                                                                                             taaattaaTTAAAACCCCACAAATAAATTAAACATAAGATTTTGCTCATTGTGTTTTATTTCTCACATTGATGACGGTCGCATGAAAAAATGATATTTTAATGAGCATTCTGAAAAAATATCGATGCATTTCGAGCGAAg                                     <  1:234038/140‑1 (MQ=255)                                                                                                          aCCCCACAAATAAATTAAACATAAGATTTTGCTCATTGTGTTTTATTTCTCACATTGATGACGGTCGCATGAAAAAATGATATTTTAATGAGCATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATcc                        >  1:415975/1‑140 (MQ=255)                                                                                                              cacaAATAAATTAAACATAAGATTTTGCTCATTGTGTTTTATTTCTCACATTGATGACGGTCGCATGAAAAAATGATATTTTAATGAGCATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGa                    <  1:113202/140‑1 (MQ=255)                                                                                                                           aaCATAAGATTTTGCTCATTGTGTTTTATTTCTCACATTGATGACGGTCGCATGAAAAAATGATATTTTAATGAGCATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTtctc       <  2:407377/140‑1 (MQ=255)                                                                                                                           aaCATAAGATTTTGCTCATTGTGTTTTATTTCTCACATTGATGACGGTCGCATGAAAAAATGATATTTTAATGAGCATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTtctc       <  2:613815/140‑1 (MQ=255)                                                                                                                                aaGATTTTGCTCATTGTGTTTTATTTCTCACATTGATGACGGTCGCATGAAAAAATGATATTTTAATGAGCATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGa  >  1:74761/1‑140 (MQ=255)                                                                                                                                  gATTTTGCTCATTGTGTTTTATTTCTCACATTGATGACGGTCGCATGAAAAAATGATATTTTAATGAGCATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGttt            <  1:35771/128‑1 (MQ=255)                                                                                                                                  gATTTTGCTCATTGTGTTTTATTTCTCACATTGATGACGGTCGCATGAAAAAATGATATTTTAATGAGCATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGttt            >  2:35771/1‑128 (MQ=255)                                                                                                                                        |                                                                                                                                    CTTTCCTGCCAGCTGCAACAATGTTGAAAATGCCAGCCAACTGCATGAAGTATGGCAAAAAGGGGCTGATGAAAATGCCAGCACAATACGATTAAATTAATTAAAACCCCACAAATAAATTAAACATAAGATTTTACTCATTGTGTTTTATTTCTCACATTGATGACGGTCGCATGAAAAAATGATATTTTAATGAGCATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGA  >  NC_000913/2741626‑2741892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑