Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,935,822	T→C	V80A (GTA→GCA)	fucI →	L‑fucose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,935,822	0	T	C	100.0%	155.6 / NA	43	V80A (GTA→GCA)	fucI	L‑fucose isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/25);  total (18/25)

AAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGTGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGC  >  NC_000913/2935687‑2935951                                                                                                                                        |                                                                                                                                  aaGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGTGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGCAGGc                                                                                                                               <  2:599984/140‑1 (MQ=255) aaGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGTGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGCAGGc                                                                                                                               <  2:607455/140‑1 (MQ=255) aaGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGTGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGCAGGc                                                                                                                               >  1:599984/1‑140 (MQ=255) aaGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGTGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGCAGGc                                                                                                                               <  1:377994/140‑1 (MQ=255)             cACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGTGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCt                                                                                                                             <  2:948468/130‑1 (MQ=255)             cACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGTGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCt                                                                                                                             >  1:948468/1‑130 (MQ=255)               ctgctgACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGTGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCTCACCATTACGGt                                                                                                                 <  2:362416/140‑1 (MQ=255)                   tgACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGTGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCTCACCATTACGGTAACg                                                                                                             <  2:816431/140‑1 (MQ=255)                    gACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGTGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCTCACCATTACGGTAACGc                                                                                                            <  1:741234/140‑1 (MQ=255)                     aCCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGTGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCTCGCCATTACGGTAACGcc                                                                                                           <  1:227607/140‑1 (MQ=255)                        gagaAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGTGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCTCACCATTACGGTAACGCCTTg                                                                                                        <  2:864909/140‑1 (MQ=255)                        gagaAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGTGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCTCACCATTACGGTAACGCCTTg                                                                                                        <  1:219777/140‑1 (MQ=255)                          gaAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGTGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCTCACCATTACGGTAACGCCTTGCt                                                                                                      >  2:312209/1‑140 (MQ=255)                                  gcCATGCCTGCGGAGCTGCCGTCGAGTGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTAt                                                                                              <  1:824869/140‑1 (MQ=255)                                                gCTGCCGTCGAGTGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCAt                                                                                <  2:750305/140‑1 (MQ=255)                                                gCTGCCGTCGAGTGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCAt                                                                                <  1:312209/140‑1 (MQ=255)                                                 cTGCCGTCGAGTGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATgg                                                                                            <  1:737180/127‑1 (MQ=255)                                                 cTGCCGTCGAGTGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATgg                                                                                            >  2:737180/1‑127 (MQ=255)                                                 cTGCCGTCGAGTGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCa                                                                                          >  2:908940/1‑129 (MQ=255)                                                 cTGCCGTCGAGTGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCa                                                                                          <  1:908940/129‑1 (MQ=255)                                                 cTGCCGTCGAGTGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATc                                                                               <  1:456307/140‑1 (MQ=255)                                                      gTCGAGTGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACAt                                                                          <  1:466018/140‑1 (MQ=255)                                                              tgtCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCaa                                                                  >  1:631297/1‑140 (MQ=255)                                                              tgtCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCaa                                                                  >  2:584489/1‑140 (MQ=255)                                                              tgtCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCaa                                                                  >  1:327649/1‑140 (MQ=255)                                                                          tACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGa                                                                       <  1:14553/123‑1 (MQ=255)                                                                          tACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGa                                                                       >  2:14553/1‑123 (MQ=255)                                                                          tACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAgg                                                      <  2:327649/140‑1 (MQ=255)                                                                                tATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGGAGGCCAttt                                                >  2:827687/1‑140 (MQ=255)                                                                                       ggTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCtt                                         >  1:897285/1‑140 (MQ=255)                                                                                        gTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCttt                                        >  2:7955/1‑140 (MQ=255)                                                                                          aTGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTaa                                      <  1:924861/140‑1 (MQ=255)                                                                                          aTGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTaa                                      <  1:785380/140‑1 (MQ=255)                                                                                          aTGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTaa                                      >  2:924861/1‑140 (MQ=255)                                                                                                  aGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTg                              >  2:212588/1‑140 (MQ=255)                                                                                                       ctgctTGCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGc                         >  1:934820/1‑140 (MQ=255)                                                                                                        tgctTGCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGcc                        >  2:56716/1‑140 (MQ=255)                                                                                                            tGCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCgg                    <  1:505258/140‑1 (MQ=255)                                                                                                            tGCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCgg                    >  1:157011/1‑140 (MQ=255)                                                                                                             gCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCgg                    <  2:146383/139‑1 (MQ=255)                                                                                                             gCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCgg                    >  1:146383/1‑139 (MQ=255)                                                                                                             gCGAAGAAAAATTCAGCAGTCAGAATGCAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGgc                   <  1:212588/140‑1 (MQ=255)                                                                                                                              agTCAGAATGCAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGc  <  2:897285/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                  AAGCTACGGCCGCACTGCTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGTGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGC  >  NC_000913/2935687‑2935951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑