Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,946,181	A→T	I333N (ATC→AAC)	mltA ←	membrane‑bound lytic murein transglycosylase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,946,181	0	A	T	100.0%	228.1 / NA	65	I333N (ATC→AAC)	mltA	membrane‑bound lytic murein transglycosylase A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (33/32);  total (33/32)

GTCATATCCGTAAAAACGGCATACAGAATATCACATCAGCCGCTAAAGACGTTACCTGCGCCCGGGGCGGTTTTCAGCACCCAGACACGTCCATAGTGGTTGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGATCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTAAATTTGCCGTTATTATCCAGCAACGGCACTTCTGCCAGCAAGGTAGT  >  NC_000913/2946046‑2946314                                                                                                                                        |                                                                                                                                      gTCATATCCGTAAAAACGGCATACAGAATATCACATCAGCCGCTAAAGACGTTACCTGCGCCCGGGGCGGTTTTCAGCACCCAGACACGTCCATAGTGGTTGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTccc                                                                                                                                   <  1:258499/140‑1 (MQ=255) gTCATATCCGTAAAAACGGCATACAGAATATCACATCAGCCGCTAAAGACGTTACCTGCGCCCGGGGCGGTTTTCAGCACCCAGACACGTCCATAGTGGTTGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTccc                                                                                                                                   <  1:568644/140‑1 (MQ=255) gTCATATCCGTAAAAACGGCATACAGAATATCACATCAGCCGCTAAAGACGTTACCTGCGCCCGGGGCGGTTTTCAGCACCCAGACACGTCCATAGTGGTTGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTccc                                                                                                                                   <  1:351390/140‑1 (MQ=255) gTCATATCCGTAAAAACGGCATACAGAATATCACATCAGCCGCTAAAGACGTTACCTGCGCCCGGGGCGGTTTTCAGCACCCAGACACGTCCATAGTGGTTGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTccc                                                                                                                                   >  1:98973/1‑140 (MQ=255)    atatCCGTAAAAACGGCATACAGAATATCACATCAGCCGCTAAAGACGTTACCTGCGCCCGGGGCGGTTTTCAGCACCCAGACACGTCCATAGTGGTTGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTg                                                                                                                                <  1:847220/140‑1 (MQ=255)      atCCGTAAAAACGGCATACAGAATATCACATCAGCCGCTAAAGATGTTACCTGCGCCCGGGGCGGTTTTCAGCACCCAGACACGTCCATAGTGGTTGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTgat                                                                                                                              <  2:479132/140‑1 (MQ=255)      atCCGTAAAAACGGCATACAGAATATCACATCAGCCGCTAAAGACGTTACCTGCGCCCGGGGCGGTTTTCAGCACCCAGACACGTCCATAGTGGTTGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTgat                                                                                                                              <  1:706652/140‑1 (MQ=255)        ccGTAAAAACGGCATACAGAATATCACATCAGCCGCTAAAGACGTTACCTGCGCCCGGGGCGGTTTTCAGCACCCAGACACGTCCATAGTGGTTGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTgatag                                                                                                                            <  2:100915/140‑1 (MQ=255)            aaaaaCGGCATACAGAATATCACATCAGCCGCTAAAGACGTTACCTGCGCCCGGGGCGGTTTTCAGCACCCAGACACGTCCATAGTGGTTGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGatat                                                                                                                        >  2:355784/1‑140 (MQ=255)              aaaCGGCATACAGAATATCACATCAGCCGCTAAAGACGTTACCTGCGCCCGGGGCGGTTTTCAGCACCCAGACACGTCCATAGTGGTTGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCg                                                                                                                      <  1:660074/140‑1 (MQ=255)                         aGAATATCACATCAGCCGCTAAAGACGTTACCTGCGCCCGGGGCGGTTTTCAGCACCCAGACACGTCCATAGTGGTTGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGcc                                                                                                           <  1:426549/140‑1 (MQ=255)                             tatCACATCAGCCGCTAAAGACGTTACCTGCGCCCGGGGCGGTTTTCAGCACCCAGACACGTCCATAGTGGTTGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTg                                                                                                       <  1:632129/140‑1 (MQ=255)                                   aTCAGCCGCTAAAGACGTTACCTGCGCCCGGGGCGGTTTTCAGCACCCAGACACGTCCATAGTGGTTGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGca                                                                                                 >  1:10061/1‑140 (MQ=255)                                    tCAGCCGCTAAAGACGTTACCTGCGCCCGGGGCGGTTTTCAGCACCCAGACACGTCCATAGTGGTTGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGcac                                                                                                <  1:279922/140‑1 (MQ=255)                                       gCCGCTAAAGACGTTACCTGCGCCCGGGGCGGTTTTCAGCACCCAGACACGTCCATAGTGGTTGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGcc                                                                                                           >  2:368172/1‑126 (MQ=255)                                       gCCGCTAAAGACGTTACCTGCGCCCGGGGCGGTTTTCAGCACCCAGACACGTCCATAGTGGTTGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGcc                                                                                                           <  1:368172/126‑1 (MQ=255)                                              aaGACGTTACCTGCGCCCGGGGCGGTTTTCAGCACCCAGACACGTCCATAGTGGTTGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATc                                                                                      <  1:409764/140‑1 (MQ=255)                                                      aCCTGCGCCCGGGGCGGTTTTCAGCACCCAGACACGTCCATAGTGGTTGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGccc                                                                              >  2:497509/1‑139 (MQ=255)                                                      aCCTGCGCCCGGGGCGGTTTTCAGCACCCAGACACGTCCATAGTGGTTGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGcca                                                                              >  2:255565/1‑140 (MQ=255)                                                      aCCTGCGCCCGGGGCGGTTTTCAGCACCCAGACACGTCCATAGTGGTTGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGcca                                                                              <  2:822941/140‑1 (MQ=255)                                                               cGGGGCGGTTTTCAGCACCCAGGCACGTCCATAGTGGTTGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCGCCACCGACATCCAGCGCCACCATCAGAc                                                                     <  2:749105/140‑1 (MQ=255)                                                               cGGGGCGGTTTTCAGCACCCAGACACGTCCATAGTGGTTGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGAc                                                                     <  1:355784/140‑1 (MQ=255)                                                               cGGGGCGGTTTTCAGCACCCAGACACGTCCATAGTGGTTGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGAc                                                                     <  1:497509/140‑1 (MQ=255)                                                                 gggCGGTTTTCAGCACCCAGACACGTCCATAGTGGTTGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGAc                                                                     >  2:83890/1‑138 (MQ=255)                                                                 gggCGGTTTTCAGCACCCAGACACGTCCATAGTGGTTGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGAc                                                                     <  1:83890/138‑1 (MQ=255)                                                                          tCAGCACCCAGACACGTCCATAGTGGTTGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTAc                                                          <  1:742694/140‑1 (MQ=255)                                                                                   aGACACGTCCATAGTGGTTGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTa                                                 >  1:940689/1‑140 (MQ=255)                                                                                   aGACACGTCCATAGTGGTTGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTa                                                 >  2:792445/1‑140 (MQ=255)                                                                                        cGTCCATAGTGGTTGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTAAAttt                                            >  1:850681/1‑140 (MQ=255)                                                                                                   gTTGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTAAATTTGc                                          >  2:897734/1‑131 (MQ=255)                                                                                                   gTTGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTAAATTTGc                                          <  1:897734/131‑1 (MQ=255)                                                                                                   gTTGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTAAATTTGCCGTtattat                                 >  1:372937/1‑140 (MQ=255)                                                                                                   gTTGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTAAATTTGCCGTtattat                                 >  1:686301/1‑140 (MQ=255)                                                                                                    ttGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTAAATTTGCCGTTATTATc                                >  1:719348/1‑140 (MQ=255)                                                                                                     tGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTAAATTTGCCGTTATTATcc                               >  1:395905/1‑140 (MQ=255)                                                                                                     tGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTAAATTTGCCGTTATTATcc                               >  2:505190/1‑140 (MQ=255)                                                                                                     tGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTAAATTTGCCGTTATTATcc                               >  1:352307/1‑140 (MQ=255)                                                                                                     tGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTAAATTTGCCGTTATTATcc                               >  1:294153/1‑140 (MQ=255)                                                                                                     tGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTAAATTTGCCGTTATTATcc                               >  2:175172/1‑140 (MQ=255)                                                                                                     tGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTCCGTACTGACCATTAAATTTGc                                          <  2:946247/129‑1 (MQ=255)                                                                                                     tGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTCCGTACTGACCATTAAATTTGc                                          >  1:946247/1‑129 (MQ=255)                                                                                                           aaCCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTAAATTTGCCGTTATTATCCAGCAAc                         >  1:870790/1‑140 (MQ=255)                                                                                                            aCCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTAAATTTGCCGTTATTATCCAGCAACg                        >  1:573310/1‑140 (MQ=255)                                                                                                            aCCTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTAAATTTGCCGTTATTATCCAGCAACg                        >  1:657857/1‑140 (MQ=255)                                                                                                             ccTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTAAATTTGCCGTTATTATCCAGCAACg                        >  1:464958/1‑139 (MQ=255)                                                                                                             ccTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTAAATTTGCCGTTATTATCCAGCAACg                        <  2:464958/139‑1 (MQ=255)                                                                                                              cTGCGCGGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTAAATTTGCCGTTATTATCCAGCAACGGc                      >  2:385468/1‑140 (MQ=255)                                                                                                                 cgcgGTGTCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTAAATTTGCCGTTATTATCCAGCAACGGCACt                   >  2:568948/1‑140 (MQ=255)                                                                                                                      tgtCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTAAATTTGCCGTTATTATCCAGCAACGGCACTTCTGc              <  2:352307/140‑1 (MQ=255)                                                                                                                       gtCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTAAATTTGCCGTTATTATCCAGCAACGGCACTTCTGcc             >  2:331069/1‑140 (MQ=255)                                                                                                                        tCCGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTAAATTTGCCGTTATTATCCAGCAACGGCACTTCTGCCa            >  1:840554/1‑140 (MQ=255)                                                                                                                         ccGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCGCCATCAGACGCAGTTCGTACTGACCATTAAATTTGCCGTTATTATCCAGCAACGGCACTTCTGCCAg           <  2:184897/140‑1 (MQ=255)                                                                                                                         ccGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTAAATTTGCCGTTATTATCCAGCAACGGCACTTCTGCCAg           <  2:372937/140‑1 (MQ=255)                                                                                                                         ccGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTAAATTTGCCGTTATTATCCAGCAACGGCACTTCTGCCAg           <  1:505190/140‑1 (MQ=255)                                                                                                                         ccGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTAAATTTGCCGTTATTATCCAGCAACGGCACTTCTGCCAg           <  2:840554/140‑1 (MQ=255)                                                                                                                         ccGGCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTAAATTTGCCGTTATTATCCAGCAACGGCACTTCTGCCAg           <  1:175172/140‑1 (MQ=255)                                                                                                                           ggCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTAAATTTGCCGTTATTATCCAGCAACGGCACTTCTGCCAGCa         <  2:657857/140‑1 (MQ=255)                                                                                                                           ggCTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTAAATTTGCCGTTATTATCCAGCAACGGCACTTCTGCCAGCa         <  2:686301/140‑1 (MQ=255)                                                                                                                             cTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTAAATTTGCCGTTATTATCCAGCAACGGCACTTCTGCCAGCa         >  1:724936/1‑138 (MQ=255)                                                                                                                             cTTCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTAAATTTGCCGTTATTATCCAGCAACGGCACTTCTGCCAGCa         <  2:724936/138‑1 (MQ=255)                                                                                                                               tCCGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTAAATTTGCCGTTATTATCCAGCAACGGCACTTCTGCCAGCAAGgt     <  2:395905/140‑1 (MQ=255)                                                                                                                                ccGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTAAATTTGCCGTTATTATCCAGCAACGGCACTTCTGCCAg           <  2:429905/133‑1 (MQ=255)                                                                                                                                ccGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTAAATTTGCCGTTATTATCCAGCAACGGCACTTCTGCCAg           >  1:429905/1‑133 (MQ=255)                                                                                                                                ccGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTAAATTTGCCGTTATTATCCAGCAACGGCACTTCTGCCAGCAAGgta    >  2:755873/1‑140 (MQ=255)                                                                                                                                ccGGCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTAAATTTGCCGTTATTATCCAGCAACGGCACTTCTACCAGCAAGgta    >  2:640013/1‑140 (MQ=255)                                                                                                                                  ggCCCGTTCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTAAATTTGCCGTTATTATCCAGCAACGGCACTTCTGCCAGCAAGgtagt  >  1:718797/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      GTCATATCCGTAAAAACGGCATACAGAATATCACATCAGCCGCTAAAGACGTTACCTGCGCCCGGGGCGGTTTTCAGCACCCAGACACGTCCATAGTGGTTGTACCAACCTGCGCGGTGTCCGGCTTCCGGCCCGATCCCTTGATAGATATCGAAGTGTTGGCCTTTGATTGCACCACCGACATCCAGCGCCACCATCAGACGCAGTTCGTACTGACCATTAAATTTGCCGTTATTATCCAGCAACGGCACTTCTGCCAGCAAGGTAGT  >  NC_000913/2946046‑2946314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑