Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,970,866 T→C G149G (GGT→GGC)  ygdQ → UPF0053 inner membrane protein YgdQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,970,8660TC100.0% 163.9 / NA 46G149G (GGT→GGC) ygdQUPF0053 inner membrane protein YgdQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/21);  total (25/21)

AGCAAGGAAATCCACGAATCCATTGAAGGTGAAGAAGAAGGGCTGAAAACACGCGTTTCATCATTCCTCGGCGCTATCGTGCAGATTATGCTGCTGGATATTATCTTTAGCCTCGACTCGGTGATTACGGCTGTGGGTCTGTCAGATCACCTGTTTATTATGATGGCAGCCGTGGTGATTGCCGTAGGCGTGATGATGTTCGCCGCGCGCTCGATTGGTGATTTTGTCGAACGCCATCCTTCGGTAAAAATGCTGGCGCTCTCTTTCCTGATT  >  NC_000913/2970729‑2971001
                                                                                                                                         |                                                                                                                                       
aGCAAGGAAATCCACGAATCCATTGAAGGTGAAGAAGAAGGGCTGAAAACACGCGTTTCATCATTCCTCGGCGCTATCGTGCAGATTATGCTGCTGGATATTATCTTTAGCCTCGACTCGGTGATTACGGCTGTGGGCCt                                                                                                                                       >  1:696167/1‑140 (MQ=255)
    aGGAAATCCACGAATCCATTGAAGGTGAAGAAGAAGGGCTGAAAACACGCGTTTCATCATTCCTCGGCGCTATCGTGCAGATTATGCTGCTGGATATTATCTTTAGCCTCGACTCGGTGATTACGGCTGTGGGCCTGTCa                                                                                                                                   <  1:348588/140‑1 (MQ=255)
     ggAAATCCACGAATCCATTGAAGGTGAAGAAGAAGGGCTGAAAACACGCGTTTCATCATTCCTCGGCGCTATCGTGCAGATTATGCTGCTGGATATTATCTTTAGCCTCGACTCGGTGATTACGGCTGTGGGCCTGTCAg                                                                                                                                  <  2:933682/140‑1 (MQ=255)
          tCCACGAATCCATTGAAGGTGAAGAAGAAGGGCTGAAAACACGCGTTTCATCATTCCTCGGCGCTATCGTGCAGATTATGCTGCTGGATATTATCTTTAGCCTCGACTCGGTGATTACGGCTGTGGGCCTGTCAGATCAc                                                                                                                             <  1:512521/140‑1 (MQ=255)
           ccACGAATCCATTGAAGGTGAAGAAGAAGGGCTGAAAACACGCGTTTCATCATTCCTCGGCGCTATCGTGCAGATTATGCTGCTGGATATTATCTTTAGCCTCGACTCGGTGATTACGACTGTGGGCCTGTCAGATCAcc                                                                                                                            <  1:739039/140‑1 (MQ=255)
               gAATCCATTGAAGGTGAAGAAGAAGGGCTGAAAACACGCGTTTCATCATTCCTCGGCGCTATCGTGCAGATTATGCTGCTGGATATTATCTTTAGCCTCGACTCGGTGATTACGGCTGTGGGCCTGTCAGATCACCTGt                                                                                                                         >  2:477602/1‑139 (MQ=255)
               gAATCCATTGAAGGTGAAGAAGAAGGGCTGAAAACACGCGTTTCATCATTCCTCGGCGCTATCGTGCAGATTATGCTGCTGGATATTATCTTTAGCCTCGACTCGGTGATTACGGCTGTGGGCCTGTCAGATCACCTGt                                                                                                                         <  1:477602/139‑1 (MQ=255)
                aaTCCATTGAAGGTGAAGAAGAAGGGCTGAAAACACGCGTTTCATCATTCCTCGGCGCTATCGTGCAGATTATGCTGCTGGATATTATCTTTAGCCTCGACTCGGTGATTACGGCTGTGGGCCTGTCAGATCAc                                                                                                                             <  1:184582/134‑1 (MQ=255)
                aaTCCATTGAAGGTGAAGAAGAAGGGCTGAAAACACGCGTTTCATCATTCCTCGGCGCTATCGTGCAGATTATGCTGCTGGATATTATCTTTAGCCTCGACTCGGTGATTACGGCTGTGGGCCTGTCAGATCAc                                                                                                                             >  2:184582/1‑134 (MQ=255)
                aaTCCATTGAAGGTGAAGAAGAAGGGCTGAAAACACGCGTTTCATCATTCCTCGGCGCTATCGTGCAGATTATGCTGCTGGATATTATCTTTAGCCTCGACTCGGTGATTACGGCTGTGGGCCTGTCAGATCACCTGttt                                                                                                                       <  1:320647/140‑1 (MQ=255)
                          aGGTGAAGAAGAAGGGCTGAAAACACGCGTTTCATCATTCCTCGGCGCTATCGTGCAGATTATGCTGCTGGATATTATCTTTAGCCTCGACTCGGTGATTACGGCTGTGGGCCTGTCAGATCACCTGTTTATTATGATgg                                                                                                             <  2:548419/140‑1 (MQ=255)
                              gaagaagaaGGGCTGAAAACACGCGTTTCATCATTCCTCGGCGCTATCGTGCAGATTATGCTGCTGGATATTATCTTTAGCCTCGACTCGGTGATTACGGCTGTGGGCCTGTCAGATCACCTGTTTATTATGATGGCa                                                                                                           <  1:145393/138‑1 (MQ=255)
                              gaagaagaaGGGCTGAAAACACGCGTTTCATCATTCCTCGGCGCTATCGTGCAGATTATGCTGCTGGATATTATCTTTAGCCTCGACTCGGTGATTACGGCTGTGGGCCTGTCAGATCACCTGTTTATTATGATGGCa                                                                                                           >  2:145393/1‑138 (MQ=255)
                                            gAAAACACGCGTTTCATCATTCCTCGGCGCTATCGTGCAGATTATGCTGCTGGATATTATCTTTAGCCTCGACTCGGTGATTACGGCTGTGGGCCTGTCAGATCACCTGTTTATTATGATGGCAGCCGTGGTGATTGCCg                                                                                           >  2:728915/1‑140 (MQ=255)
                                            gAAAACACGCGTTTCATCATTCCTCGGCGCTATCGTGCAGATTATGCTGCTGGATATTATCTTTAGCCTCGACTCGGTGATTACGGCTGTGGGCCTGTCAGATCACCTGTTTATTATGATGGCAGCCGTGGTGATTGCCg                                                                                           <  2:696167/140‑1 (MQ=255)
                                                  acGCGTTTCATCATTCCTCGGCGCTATCGTGCAGATTATGCTGCTGGATATTATCTTTAGCCTCGACTCGGTGATTACGGCTGTGGGCCTGTCAGATCACCTGTTTATTATGATGGCAGCCGTGGTGATTGCCGTAGGCg                                                                                     >  1:6735/1‑140 (MQ=255)
                                                  acGCGTTTCATCATTCCTCGGCGCTATCGTGCAGATTATGCTGCTGGATATTATCTTTAGCCTCGACTCGGTGATTACGGCTGTGGGCCTGTCAGATCACCTGTTTATTATGATGGCAGCCGTGGTGATTGCCGTAGGCg                                                                                     >  2:131137/1‑140 (MQ=255)
                                                      gTTTTAGCATTCCTCCACGCTAGCGTGGAGATTGTGCTGCTGGATATGATCTTTAGCCTCGACTCGGTGATTACGGCTGTGGGCCTGTCAGATCACCTGTTTATTATGATGGCAGCCGTGGTGATTGCCGTAGGCGtgat                                                                                 <  2:276328/140‑1 (MQ=255)
                                                                 ccTCGGCGCTATCGTGCAGATTATGCTGCTGGATATTATCTTTAGCCTCGACTCGGTGATTACGGCTGTGGGCCTGTCAGATCACCTGTTTATTATGATGGCAGCCGTGGTGATTGCCGTAGGCGTGATGATGTTcgccg                                                                      <  1:728915/140‑1 (MQ=255)
                                                                  cTCGGCGCTATCGTGCAGATTATGCTGCTGGATATTATCTTTAGCCTCGACTCGGTGATTACGGCTGTGGGCCTGTCAGATCACCTGTTTATTATGATGGCAGCCGTGGTGATTGCCGTAGGCGTGATGATGTTcgccgc                                                                     >  1:615161/1‑140 (MQ=255)
                                                                    cGGCGCTATCGTGCAGATTATGCTGCTGGATATTATCTTTAGCCTCGACTCGGTGATTACGGCTGTGGGCCTGTCAGATCACCTGTTTATTATGATGGCAGCCGTGGTGATTGCCGTAGGCGTGATGATGTTCGCcgcgc                                                                   >  1:373349/1‑140 (MQ=255)
                                                                    cGGCGCTATCGTGCAGATTATGCTGCTGGATATTATCTTTAGCCTCGACTCGGTGATTACGGCTGTGGGCCTGTCAGATCACCTGTTTATTATGATGGCAGCCGTGGTGATTGCCGTAGGCGTGATGATGTTCGCcgcgc                                                                   >  2:387908/1‑140 (MQ=255)
                                                                         cTATCGTGCAGATTATGCTGCTGGATATTATCTTTAGCCTCGACTCGGTGATTACGGCTGTGGGCCTGTCAGATCACCTGTTTATTATGATGGCAGCCGTGGTGATTGCCGTAGGCGTGATGATGTTCGCCGCGCGCTCg                                                              >  1:888415/1‑140 (MQ=255)
                                                                          tATCGTGCAGATTATGCTGCTGGATATTATCTTTAGCCTCGACTCGGTGATTACGGCTGTGGGCCTGTCAGATCACCTGTTTATTATGATGGCAGCCGTGGTGATTGCCGTAGGCGTGATGATGTTCGCcgcgcg                                                                  <  1:107883/135‑1 (MQ=255)
                                                                          tATCGTGCAGATTATGCTGCTGGATATTATCTTTAGCCTCGACTCGGTGATTACGGCTGTGGGCCTGTCAGATCACCTGTTTATTATGATGGCAGCCGTGGTGATTGCCGTAGGCGTGATGATGTTCGCcgcgcg                                                                  >  2:107883/1‑135 (MQ=255)
                                                                            tCGTGCAGATTATGCTGCTGGATATTATCTTTAGCCTCGACTCGGTGATTACGGCTGTGGGCCTGTCAGATCACCTGTTTATTATGATGGCAGCCGTGGTGATTGCCGTAGGCGTGATGATGTTCGCCGCGCGCTCGAtt                                                           >  2:369469/1‑140 (MQ=255)
                                                                                      tATGCTGCTGGATATTATCTTTAGCCTCGACTCGGTGATTACGGCTGTGGGCCTGTCAGATCACCTGTTTATTATGATGGCAGCCGTGGTGATTGCCGTAGGCGTGATGATGTTCGCCGCGCGCTCGATTGGTGATTTTg                                                 >  2:110028/1‑140 (MQ=255)
                                                                                      tATGCTGCTGGATATTATCTTTAGCCTCGACTCGGTGATTACGGCTGTGGGCCTGTCAGATCACCTGTTTATTATGATGGCAGCCGTGGTGATTGCCGTAGGCGTGATGATGTTCGCCGCGCGCTCGATTGGTGATTTTg                                                 >  2:218355/1‑140 (MQ=255)
                                                                                      tATGCTGCTGGATATTATCTTTAGCCTCGACTCGGTGATTACGGCTGTGGGCCTGTCAGATCACCTGTTTATTATGATGGCAGCCGTGGTGATTGCCGTAGGCGTGATGATGTTCGCCGCGCGCTCGATTGGTGATTTTg                                                 <  2:615161/140‑1 (MQ=255)
                                                                                        tgctgcTGGATATTATCTTTAGCCTCGACTCGGTGATTACGGCTGTGGGCCTGTCAGATCACCTGTTTATTATGATGGCAGCCGTGGTGATTGCCGTAGGCGTGATGATGTTCGCCGCGCGCTCGATTGGTGATTTTGTc                                               <  2:112584/140‑1 (MQ=255)
                                                                                        tgctgcTGGATATTATCTTTAGCCTCGACTCGGTGATTACGGCTGTGGGCCTGTCAGATCACCTGTTTATTATGATGGCAGCCGTGGTGATTGCCGTAGGCGTGATGATGTTCGCCGCGCGCTCGATTGGTGATTTTGTc                                               <  1:533724/140‑1 (MQ=255)
                                                                                          ctgctgGATATTATCTTTAGCCTCGACTCGGTGATTACGGCTGTGGGCCTGTCAGATCACCTGTTTATTATGATGGCAGCCGTGGTGATTGCCGTAGGCGTGATGATGTTCGCCGCGCGCTCGATTGGTGATTTTGTCGa                                             <  2:584985/140‑1 (MQ=255)
                                                                                             ctgGATATTATCTTTAGCCTCGACTCGGTGATTACGGCTGTGGGCCTGTCAGATCACCTGTTTATTATGATGGCAGCCGTGGTGATTGCCGTAGGCGTGATGATGTTCGCCGCGCGCTCGATTGGTGATTTTGTCGAACg                                          >  1:338970/1‑140 (MQ=255)
                                                                                              tgGATATTATCTTTAGCCTCGACTCGGTGATTACGGCTGTGGGCCTGTCAGATCACCTGTTTATTATGATGGCAGCCGTGGTGATTGCCGTAGGCGTGATGATGTTCGCCGCGCGCTCGATTGGTGATTTTGTCGAACGc                                         >  1:596629/1‑140 (MQ=255)
                                                                                                gATATTATCTTTAGCCTCGACTCGGTGATTACGGCTGTGGGCCTGTCAGATCACCTGTTTATTATGATGGCAGCCGTGGTGATTGCCGTAGGCGTGATGATGTTCGCCGCGCGCTCGATTGGTGATTTTGTCGAACGCCa                                       >  1:559143/1‑140 (MQ=255)
                                                                                                     tatCTTTAGCCTCGACTCGGTGATTACGGCTGTGGGCCTGTCAGATCACCTGTTTATTATGATGGCAGCCGTGGTGATTGCCGTAGGCGTGATGATGTTCGCCGCGCGCTCGATTGGTGATTTTGTCGAACGCCATCCtt                                  <  2:596629/140‑1 (MQ=255)
                                                                                                         tttAGCCTCGACTCGGTGATTACGGCTGTGGGCCTGTCAGATCACCTGTTTATTATGATGGCAGCCGTGGTGATTGCCGTAGGCGTGATGATGTTCGCCGCGCGCTCGATTGGTGATTTTGTCGAACGCCATCCTTCGGt                              <  2:338970/140‑1 (MQ=255)
                                                                                                                 cGACTCGGTGATTACGGCTGTGGGCCTGTCAGATCACCTGTTTATTATGATGGCAGCCGTGGTGATTGCCGTAGGCGTGATGATGTTCGCCGCGCGCTCGATTGGTGATTTTGTCGAACGCCATCCTTCGGTAAAAATGc                      >  2:686711/1‑140 (MQ=255)
                                                                                                                       ggTGATTACGGCTGTGGGCCTGTCAGATCACCTGTTTATTATGATGGCAGCCGTGGTGATTGCCGTAGGCGTGATGATGTTCGCCGCGCGCTCGATTGGTGATTTTGTCGAACGCCATCCTTCGGTAAAAATGCTGgcgc                <  1:201397/140‑1 (MQ=255)
                                                                                                                       ggTGATTACGGCTGTGGGCCTGTCAGATCACCTGTTTATTATGATGGCAGCCGTGGTGATTGCCGTAGGCGTGATGATGTTCGCCGCGCGCTCGATTGGTGATTTTGTCGAACGCCATCCTTCGGTAAAAATGCTGgcgc                <  1:369469/140‑1 (MQ=255)
                                                                                                                        gTGATTACGGCTGTGGGCCTGTCAGATCACCTGTTTATTATGATGGCAGCCGTGGTGATTGCCGTAGGCGTGATGATGTTCGCCGCGCGCTCGATTGGTGATTTTGTCGAACGCCATCCTTCGGTAAAAATGCTGGCGct               >  1:400755/1‑140 (MQ=255)
                                                                                                                        gTGATTACGGCTGTGGGCCTGTCAGATCACCTGTTTATTATGATGGCAGCCGTGGTGATTGCCGTAGGCGTGATGATGTTCGCCGCGCGCTCGATTGGTGATTTTGTCGAACGCCATCCTTCGGTAAAAATGCTGGCGct               >  2:716491/1‑140 (MQ=255)
                                                                                                                        gTGATTACGGCTGTGGGCCTGTCAGATCACCTGTTTATTATGATGGCAGCCGTGGTGATTGCCGTAGGCGTGATGATGTTCGCCGCGCGCTCGATTGGTGATTTTGTCGAACGCCATCCTTCGGTAAAAATGCTGGCGct               <  1:110028/140‑1 (MQ=255)
                                                                                                                                 gCTGTGGGCCTGTCAGATCACCTGTTTATTATGATGGCAGCCGTGGTGATTGCCGTAGGCGTGATGATGTTCGCCGCGCGCTCGATTGGTGATTTTGTCGAACGCCATCCTTCGGTAAAAATGCTGGCGCTCTCTTTCCt      >  1:14680/1‑140 (MQ=255)
                                                                                                                                   tgtgGGCCTGTCAGATCACCTGTTTATTATGATGGCAGCCGTGGTGATTGCCGTAGGCGTGATGATGTTCGCCGCGCGCTCGATTGGTGATTTTGTCGAACGCCATCCTTCGGTAAAAATGCTGGCGCTCTCTTTCCTGa    >  1:769352/1‑140 (MQ=255)
                                                                                                                                     tgGGCCTGTCAGATCACCTGTTTATTATGATGGCAGCCGTGGTGATTGCCGTAGGCGTGATGATGTTCGCCGCGCGCTCGATTGGTGATTTTGTCGAACGCCATCCTTCGGTAAAAATGCTGGCGCTCTCTTTCCTGAtt  >  1:116287/1‑140 (MQ=255)
                                                                                                                                     tgGGCCTGTCAGATCACCTGTTTATTATGATGGCAGCCGTGGTGATTGCCGTAGGCGTGATGATGTTCGCCGCGCGCTCGATTGGTGATTTTGTCGAACGCCATCCTTCGGTAAAAATGCTGGCGCTCTCTTTCCTGAtt  >  1:283915/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                       
AGCAAGGAAATCCACGAATCCATTGAAGGTGAAGAAGAAGGGCTGAAAACACGCGTTTCATCATTCCTCGGCGCTATCGTGCAGATTATGCTGCTGGATATTATCTTTAGCCTCGACTCGGTGATTACGGCTGTGGGTCTGTCAGATCACCTGTTTATTATGATGGCAGCCGTGGTGATTGCCGTAGGCGTGATGATGTTCGCCGCGCGCTCGATTGGTGATTTTGTCGAACGCCATCCTTCGGTAAAAATGCTGGCGCTCTCTTTCCTGATT  >  NC_000913/2970729‑2971001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑