Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,995,766	T→C	pseudogene (255/707 nt)	ygeN ←	putative type III secretion system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,995,766	0	T	C	100.0%	255.0 / NA	70	pseudogene (255/707 nt)	ygeN	putative type III secretion system protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/36);  total (34/36)

AGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGTTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGT  >  NC_000913/2995630‑2995900                                                                                                                                         |                                                                                                                                       agaTTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGctct                                                                                                                                     >  2:907149/1‑140 (MQ=255)   aTTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGa                                                                                                                                   >  1:885882/1‑140 (MQ=255)   aTTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGa                                                                                                                                   >  2:320662/1‑140 (MQ=255)   aTTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGa                                                                                                                                   >  1:512831/1‑140 (MQ=255)    tttACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAg                                                                                                                                  >  2:554348/1‑140 (MQ=255)    tttACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAg                                                                                                                                  <  2:512831/140‑1 (MQ=255)    tttACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAg                                                                                                                                  >  2:246745/1‑140 (MQ=255)       aCAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCAt                                                                                                                               >  2:674866/1‑140 (MQ=255)        cAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATa                                                                                                                              >  2:669179/1‑140 (MQ=255)         aGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAg                                                                                                                             <  2:576547/140‑1 (MQ=255)         aGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAg                                                                                                                             <  1:246745/140‑1 (MQ=255)           gACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAg                                                                                                                                  >  2:637194/1‑133 (MQ=255)           gACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAg                                                                                                                                  <  1:637194/133‑1 (MQ=255)             ccTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAg                                                                                                                         >  2:428513/1‑140 (MQ=255)             ccTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAg                                                                                                                         >  2:546786/1‑140 (MQ=255)             ccTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAg                                                                                                                         <  1:554348/140‑1 (MQ=255)                gATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCtt                                                                                                                      >  1:328839/1‑140 (MQ=255)                   atTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATc                                                                                                                   <  2:328839/140‑1 (MQ=255)                     tGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCaa                                                                                                                 <  2:685581/140‑1 (MQ=255)                     tGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCaa                                                                                                                 <  2:2669/140‑1 (MQ=255)                     tGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCa                                                                                                                  <  1:68270/139‑1 (MQ=255)                     tGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCa                                                                                                                  >  2:68270/1‑139 (MQ=255)                       aTGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAgg                                                                                                               >  2:478122/1‑140 (MQ=255)                       aTGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAgg                                                                                                               >  1:549126/1‑140 (MQ=255)                        tGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAggg                                                                                                              >  2:109273/1‑140 (MQ=255)                         ggTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGt                                                                                                             >  2:57748/1‑140 (MQ=255)                          gTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGta                                                                                                            >  1:354784/1‑140 (MQ=255)                          gTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGta                                                                                                            <  1:546786/140‑1 (MQ=255)                                   ttACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTAt                                                                                                   >  2:627127/1‑140 (MQ=255)                                     aCCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATcc                                                                                                 <  1:669179/140‑1 (MQ=255)                                     aCCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATcc                                                                                                 >  2:574700/1‑140 (MQ=255)                                     aCCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATcc                                                                                                 <  1:674866/140‑1 (MQ=255)                                      cccAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCa                                                                                                >  2:28512/1‑140 (MQ=255)                                      cccAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCa                                                                                                >  2:19331/1‑140 (MQ=255)                                       ccAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCAt                                                                                               <  2:602551/140‑1 (MQ=255)                                       ccAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCAt                                                                                               <  1:28512/140‑1 (MQ=255)                                       ccAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCAt                                                                                               <  1:907149/140‑1 (MQ=255)                                         aaCTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCAtct                                                                                             >  1:932995/1‑140 (MQ=255)                                                   aaTTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCaataat                                                                                   <  1:109273/140‑1 (MQ=255)                                                     ttttGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATc                                                                                                  <  2:461027/123‑1 (MQ=255)                                                     ttttGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATc                                                                                                  >  1:461027/1‑123 (MQ=255)                                                       ttGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCt                                                                               <  1:597576/140‑1 (MQ=255)                                                             aaaaCATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGta                                                                         <  1:574700/140‑1 (MQ=255)                                                              aaaCATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGtat                                                                        <  2:875387/140‑1 (MQ=255)                                                                  aTCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGcc                                                                    <  1:777529/140‑1 (MQ=255)                                                                  aTCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGcc                                                                    <  1:298183/140‑1 (MQ=255)                                                                   tCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCca                                                                   <  1:57748/140‑1 (MQ=255)                                                                   tCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCca                                                                   <  1:673852/140‑1 (MQ=255)                                                                    cAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCcat                                                                  <  1:428513/140‑1 (MQ=255)                                                                    cAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCcat                                                                  <  1:728481/140‑1 (MQ=255)                                                                    cAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCcat                                                                  <  1:19331/140‑1 (MQ=255)                                                                            tctGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCAtaataa                                                          >  1:689916/1‑140 (MQ=255)                                                                                             tataCTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTttc                                         >  1:2972/1‑140 (MQ=255)                                                                                             tataCTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCAAAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTttc                                         >  2:231935/1‑140 (MQ=255)                                                                                                 ctctTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTtcttct                                     <  2:689916/140‑1 (MQ=255)                                                                                                    ttCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTtctctc                                  <  2:932995/140‑1 (MQ=255)                                                                                                     tCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTctctct                                 >  2:146901/1‑140 (MQ=255)                                                                                                      cAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCtt                                <  2:69276/140‑1 (MQ=255)                                                                                                        gATCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCACAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCtttt                              <  2:885882/140‑1 (MQ=255)                                                                                                         aTCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTc                             >  1:86522/1‑140 (MQ=255)                                                                                                         aTCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTc                             >  1:417600/1‑140 (MQ=255)                                                                                                         aTCGTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTc                             >  1:177924/1‑140 (MQ=255)                                                                                                            gTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTc                          >  2:451694/1‑140 (MQ=255)                                                                                                            gTTTATCATGTTTATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTc                          >  2:858474/1‑140 (MQ=255)                                                                                                                        tATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCtt                                <  2:232512/122‑1 (MQ=255)                                                                                                                        tATTCTTTTTTTTCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCtt                                >  1:232512/1‑122 (MQ=255)                                                                                                                              tttttttCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGc        <  1:146901/140‑1 (MQ=255)                                                                                                                               ttttttCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCg       <  1:83943/140‑1 (MQ=255)                                                                                                                                tttttCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCg       >  2:421907/1‑139 (MQ=255)                                                                                                                                tttttCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCg       <  1:421907/139‑1 (MQ=255)                                                                                                                                    tCAAGCTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCggtggt  <  2:354784/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       AGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATATACTCTTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGTTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGT  >  NC_000913/2995630‑2995900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑