Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	286,341	A→G	H316R (CAC→CGC)	yagG →	putative D‑xylonate transporter YagG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	286,341	0	A	G	100.0%	130.6 / NA	37	H316R (CAC→CGC)	yagG	putative D‑xylonate transporter YagG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/22);  total (15/22)

CACCATGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCACATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCAGCGGTCGCCGCCTCGACGGGCTGGTG  >  NC_000913/286216‑286477                                                                                                                              |                                                                                                                                         caccaTGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCGCATCGCGCTCAttt                                                                                                                            <  1:888775/140‑1 (MQ=255)     aTGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCGCATCGCGCTCATTTTTGc                                                                                                                        <  2:752861/140‑1 (MQ=255)      tGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCGCATCGCGCTCATTTTTGcc                                                                                                                       >  1:628447/1‑140 (MQ=255)       gTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCGCATCGCGCTCATTTTTGccc                                                                                                                      <  1:630699/140‑1 (MQ=255)           ggCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCGCATCGCGCTCATTTTTGCCCTCaa                                                                                                                  <  2:841745/140‑1 (MQ=255)           ggCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCGCATCGCGCTCATTTTTGCCCTCaa                                                                                                                  <  1:83684/140‑1 (MQ=255)                           cACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCGCATCGCGCTCATTTTTGCCCTCAACATCctgttcctgttc                                                                                                  <  1:943112/140‑1 (MQ=255)                           cACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCGCATCGCGCTCATTTTTGCCCTCAACATCctgttcctgttc                                                                                                  <  1:386815/140‑1 (MQ=255)                              gCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCGCATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTc                                                                                               >  1:514143/1‑140 (MQ=255)                                 tgctgGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCGCATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCttt                                                                                            >  2:825919/1‑140 (MQ=255)                                   ctgGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCGCATCGCGCTCATTTTTGCCCTCAACATCctgttcctgttc                                                                                                  <  2:731274/132‑1 (MQ=255)                                   ctgGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCGCATCGCGCTCATTTTTGCCCTCAACATCctgttcctgttc                                                                                                  >  1:731274/1‑132 (MQ=255)                                           cTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCGCATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATAccacca                                                                                  >  2:28933/1‑140 (MQ=255)                                               gACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCGCATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCAcgcc                                                                              <  2:514143/140‑1 (MQ=255)                                                aCCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCGCATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCAcgccg                                                                             <  2:628447/140‑1 (MQ=255)                                                  cgcgTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCGCATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCt                                                                           <  1:825919/140‑1 (MQ=255)                                                    cgTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCGCATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGc                                                                         >  2:777140/1‑140 (MQ=255)                                                         ccGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCGCATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTgg                                                                    <  2:777852/140‑1 (MQ=255)                                                               tCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCGCATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATg                                                              >  2:617789/1‑140 (MQ=255)                                                                cAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCGCATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATgg                                                             >  2:896503/1‑140 (MQ=255)                                                                      gATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCGCATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTg                                                       <  1:867319/140‑1 (MQ=255)                                                                       atcatcGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCGCATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGa                                                      >  1:188076/1‑140 (MQ=255)                                                                              tcgCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCGCATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACgtggtg                                               >  1:189370/1‑140 (MQ=255)                                                                                     cTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCGCATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACg                                                    <  1:74479/128‑1 (MQ=255)                                                                                     cTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCGCATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACg                                                    >  2:74479/1‑128 (MQ=255)                                                                                     cTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCGCATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACg                                        >  2:353924/1‑140 (MQ=255)                                                                                       cGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCGCATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTTCgag                                      <  1:617789/140‑1 (MQ=255)                                                                                          tGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCGCATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACgaggag                                   <  1:28933/140‑1 (MQ=255)                                                                                                 ccTGCTGATTTTCGTCACCCCGGCGGAGCGCATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCa                            >  1:696161/1‑140 (MQ=255)                                                                                                  ctgctgATTTTCGTCACCCCGGCGGAGCGCATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTGATACCACCACGCCGCTGCAGTGGCTGATGGATTCTGACGTGGTGGTCTACGAGGAGAGCCGCAg                           >  2:755119/1‑140 (MQ=255)                                                                                                        aTTTTCGTCACCCCGGCGGAGCGCATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCAGCGGTcg                     <  1:670085/140‑1 (MQ=255)                                                                                                                cACCCCGGCGGAGCGCATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCAGCGGTCGCCGCCTCg             <  1:777140/140‑1 (MQ=255)                                                                                                                 aCCCCGGCGGAGCGCATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCAGCGGTCGCCGCCTCGa            <  1:353924/140‑1 (MQ=255)                                                                                                                  ccccGGCGGAGCGCATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCAGCGGTCGCCGCCTCGAc           >  1:112605/1‑140 (MQ=255)                                                                                                                   cccGGCGGAGCGCATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCAGCGGTCGCCGCCTCGACg          <  2:647090/140‑1 (MQ=255)                                                                                                                           aGCGCATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCAGCGGTCGCCGCCTCGACGGGCTGgtg  <  2:188076/140‑1 (MQ=255)                                                                                                                           aGCGCATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCAGCGGTCGCCGCCTCGACGGGCTGgtg  <  1:896503/140‑1 (MQ=255)                                                                                                                              |                                                                                                                                         CACCATGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCGGAGCACATCGCGCTCATTTTTGCCCTCAACATCCTGTTCCTGTTCGTCTTTAATACCACCACGCCGCTGCAGTGGCTGATGGCTTCTGACGTGGTGGACTACGAGGAGAGCCGCAGCGGTCGCCGCCTCGACGGGCTGGTG  >  NC_000913/286216‑286477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑