Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,026,670	A→G	N302S (AAC→AGC)	guaD →	guanine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,026,670	0	A	G	100.0%	102.6 / NA	29	N302S (AAC→AGC)	guaD	guanine deaminase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/11);  total (18/11)

TTATCTGGATGTTTACCATCAGTACGGCCTGACCGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAA  >  NC_000913/3026536‑3026799                                                                                                                                       |                                                                                                                                  ttATCTGGATGTTTACCATCAGTACGGCCTGACCGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCttt                                                                                                                              >  1:702775/1‑140 (MQ=255)              tACCATCAGTACGGCCTGACCGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCgg                                                                                                                 >  1:574142/1‑140 (MQ=255)                       tACGGCCTGACCGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCaa                                                                                                        >  1:173232/1‑140 (MQ=255)                       tACGGCCTGACCGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCaa                                                                                                        >  2:827682/1‑140 (MQ=255)                             cTGACCGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGaa                                                                                                  >  1:595537/1‑140 (MQ=255)                             cTGACCGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTCTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGaa                                                                                                  <  2:652921/140‑1 (MQ=255)                               gACCGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGaaaa                                                                                                >  2:780395/1‑140 (MQ=255)                               gACCGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGaaaa                                                                                                >  1:810597/1‑140 (MQ=255)                                 ccGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAAc                                                                                                       <  2:846736/131‑1 (MQ=255)                                 ccGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAAc                                                                                                       >  1:846736/1‑131 (MQ=255)                                    gTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCa                                                                                           <  2:574142/140‑1 (MQ=255)                                       aaaaCTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATgg                                                                                        <  2:173232/140‑1 (MQ=255)                                                            gCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGc                                                                   >  2:206156/1‑140 (MQ=255)                                                               tCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATg                                                                >  2:246683/1‑140 (MQ=255)                                                               tCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATg                                                                >  2:875699/1‑140 (MQ=255)                                                                ccATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCTGAAGAAAGTTAAAGTGGGCATgg                                                               >  1:731442/1‑140 (MQ=255)                                                                ccATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATgg                                                               >  1:454387/1‑140 (MQ=255)                                                                       gaagaaAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGa                                                        >  1:553461/1‑140 (MQ=255)                                                                                           tctcAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACtt                                    >  2:703010/1‑140 (MQ=255)                                                                                                    aaCCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGaa                                          <  2:421763/125‑1 (MQ=255)                                                                                                    aaCCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGaa                                          >  1:421763/1‑125 (MQ=255)                                                                                                    aaCCAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACAtgc                           >  2:244909/1‑140 (MQ=255)                                                                                                       cAAATCCAGCATTGCTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACAtgctgc                        <  2:86931/140‑1 (MQ=255)                                                                                                                     cTTTCTGTCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGaa          <  1:206156/140‑1 (MQ=255)                                                                                                                            tCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCTGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACa   <  2:731442/140‑1 (MQ=255)                                                                                                                            tCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACa   <  1:875699/140‑1 (MQ=255)                                                                                                                            tCCGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACa   <  1:726253/140‑1 (MQ=255)                                                                                                                             ccGACCTCCAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACaa  >  2:906537/1‑140 (MQ=255)                                                                                                                                    ccAGCCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACa   <  1:193389/132‑1 (MQ=255)                                                                                                                                       |                                                                                                                                  TTATCTGGATGTTTACCATCAGTACGGCCTGACCGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTACAA  >  NC_000913/3026536‑3026799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑