Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,144,286	A→G	R285R (CGT→CGC)	hybA ←	hydrogenase 2 iron‑sulfur protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,144,286	0	A	G	100.0%	243.9 / NA	68	R285R (CGT→CGC)	hybA	hydrogenase 2 iron‑sulfur protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/35);  total (33/35)

GTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGCGACGAACCAGCACGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCACC  >  NC_000913/3144149‑3144415                                                                                                                                          |                                                                                                                                  gtgGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGCGACGAACCAGCACGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGcg                                                                                                                                 >  2:40081/1‑140 (MQ=255) gtgGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGCGACGAACCAGCACGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGcg                                                                                                                                 >  2:9946/1‑140 (MQ=255)   ggATCATGACTCATGTTCGTTCCCTCCGTCGTGATGGTTGTTTTTGGTGTTGCGACGAACCAGCCCGTTTAACCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGcgcg                                                                                                                               <  2:437854/140‑1 (MQ=255)   ggATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGCGACGAACCAGCACGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGcgcg                                                                                                                               <  1:562934/140‑1 (MQ=255)   ggATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGCGACGAACCAGCACGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGcgcg                                                                                                                               >  2:721264/1‑140 (MQ=255)   ggATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGCGACGAACCAGCACGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGcgcg                                                                                                                               <  1:728901/140‑1 (MQ=255)   ggATCATGACTAATGTACGTCCCCTCCGTCGTGATGGGCGTTTTGGGGGTGGAGACGAACCAGAACGGTAAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGcgcg                                                                                                                               <  2:841459/140‑1 (MQ=255)     aTCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGCGACGAACCAGCACGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGCGCGCa                                                                                                                             <  1:9946/140‑1 (MQ=255)     aTCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGCGACGAACCAGCACGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGCGCGCa                                                                                                                             <  2:645321/140‑1 (MQ=255)      tCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGCGACGAACCAGCACGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGCGCGCAc                                                                                                                            <  1:40081/140‑1 (MQ=255)         tGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGCGACGAACCAGCACGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGCGCGCACCgg                                                                                                                         >  1:389259/1‑140 (MQ=255)         tGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGCGACGAACCAGCACGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGCGCGCACCgg                                                                                                                         >  1:437854/1‑140 (MQ=255)            cTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGCGACGAACCAGCACGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGCGCGCACCGGTAg                                                                                                                      <  2:532171/140‑1 (MQ=255)                 gATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGCGACGAACCAGCACGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGCGCGCACCGGTAGAAAGa                                                                                                                 >  2:255815/1‑140 (MQ=255)                    cGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGCGACGAACCAGCACGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGCGCGCAcc                                                                                                                           <  1:423192/127‑1 (MQ=255)                    cGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGCGACGAACCAGCACGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGCGCGCAcc                                                                                                                           >  2:423192/1‑127 (MQ=255)                       ctcctcCGTCGTGATGGTCG‑TTTTGGTGTTGCGACGAACCAGCACGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCa                                                                                                          >  1:6470/1‑140 (MQ=255)                             cgtcgtGATGGTCGTTTTTGGTGTTGCGACGAACCAGCACGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTc                                                                                                     >  1:617604/1‑140 (MQ=255)                                cgtGATGGTCGTTTTTGGTGTTGCGACGAACCAGCACGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAG‑TTCGGCa                                                                                                 >  1:663404/1‑140 (MQ=255)                                 gtGATGGTCGTTTTTGGTGTTGCGACGAACCAGCACGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCa                                                                                                 >  1:489067/1‑140 (MQ=255)                                    aTGGTCGTTTTTGGGGTTGCGACGAACCAGCACGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGt                                                                                              >  2:704508/1‑140 (MQ=255)                                          ctttttGGTGTGGCAACGACCCCGCACAGTCCAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAG‑TTCGGCAGGTCGAGAtt                                                                                       <  2:663404/139‑1 (MQ=255)                                               tGGTGTTGCGACGAACCAGCACGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCa                                                                                   <  1:583233/140‑1 (MQ=255)                                                      gCGACGAACCAGCACGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGca                                                                            >  1:185857/1‑140 (MQ=255)                                                         acgaACCAGCACGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCacac                                                                         <  1:788342/140‑1 (MQ=255)                                                             aCCAGCACGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGt                                                                     <  2:185857/140‑1 (MQ=255)                                                             aCCAGCACGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGt                                                                     <  2:489067/140‑1 (MQ=255)                                                              ccAGCACGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTc                                                                    <  1:392692/140‑1 (MQ=255)                                                                    cGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTAcc                                                              <  1:245020/140‑1 (MQ=255)                                                                       ttAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCacac                                                                         >  2:863686/1‑126 (MQ=255)                                                                       ttAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCacac                                                                         <  1:863686/126‑1 (MQ=255)                                                                       ttAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGt                                                                     >  1:107721/1‑130 (MQ=255)                                                                       ttAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGt                                                                     <  2:107721/130‑1 (MQ=255)                                                                       ttAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTGTCGGAGCGCGCACCGGTAGAAAGATCGACCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGa                                                           >  2:848628/1‑140 (MQ=255)                                                                              cGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAg                                                    <  2:389259/140‑1 (MQ=255)                                                                               gCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGt                                                   >  2:946698/1‑140 (MQ=255)                                                                                   gCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTAccg                                               <  2:6470/140‑1 (MQ=255)                                                                                        gCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCgccgcc                                          <  2:434338/140‑1 (MQ=255)                                                                                               gTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTtc                                   <  1:848628/140‑1 (MQ=255)                                                                                                tAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTtct                                  <  1:255815/140‑1 (MQ=255)                                                                                                  gCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCg                                <  1:721264/140‑1 (MQ=255)                                                                                                       atGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTa                           >  2:679580/1‑140 (MQ=255)                                                                                                        tGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCgccgcc                                          >  2:669347/1‑124 (MQ=255)                                                                                                        tGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCgccgcc                                          <  1:669347/124‑1 (MQ=255)                                                                                                        tGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTAc                          >  1:17654/1‑140 (MQ=255)                                                                                                        tGCCTTTATACAGGGTGTGTTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTAc                          >  2:727276/1‑140 (MQ=255)                                                                                                            tttATACAGGGTGTGTTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGAt                      <  2:617604/140‑1 (MQ=255)                                                                                                                  cAGGGTGTGTTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGTCGCCCTCTTTCTCGCCGTACAGATGCGGat                <  2:123178/140‑1 (MQ=255)                                                                                                                     ggTGTGTTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATg                     >  1:879273/1‑132 (MQ=255)                                                                                                                     ggTGTGTTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATg                     <  2:879273/132‑1 (MQ=255)                                                                                                                     ggTGTGTTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGataat             >  1:321337/1‑140 (MQ=255)                                                                                                                     ggTGTGTTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGataat             >  1:346318/1‑140 (MQ=255)                                                                                                                      gtgtgtTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTCAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGataata            >  2:882895/1‑140 (MQ=255)                                                                                                                       tgtgtTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAatat           >  1:206588/1‑140 (MQ=255)                                                                                                                       tgtgtTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAatat           >  1:841839/1‑140 (MQ=255)                                                                                                                        gtgtTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACa                         <  1:861930/125‑1 (MQ=255)                                                                                                                        gtgtTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACa                         >  2:861930/1‑125 (MQ=255)                                                                                                                         tgtTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATAttt         >  2:295173/1‑140 (MQ=255)                                                                                                                         tgtTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATAttt         >  1:533750/1‑140 (MQ=255)                                                                                                                          gtTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGAt                      <  2:471438/126‑1 (MQ=255)                                                                                                                          gtTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGAt                      >  1:471438/1‑126 (MQ=255)                                                                                                                          gtTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTc        >  1:559145/1‑140 (MQ=255)                                                                                                                          gtTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTc        >  1:469974/1‑140 (MQ=255)                                                                                                                          gtTGAATATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTc        >  1:742904/1‑140 (MQ=255)                                                                                                                              aaTATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCa    <  2:841839/140‑1 (MQ=255)                                                                                                                              aaTATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCa    <  1:882895/140‑1 (MQ=255)                                                                                                                              aaTATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCa    <  1:230533/140‑1 (MQ=255)                                                                                                                              aaTATTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCa    <  2:17654/140‑1 (MQ=255)                                                                                                                                tatTTTCGGAGCGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCAcc  <  1:704508/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                  GTGGATCATGACTCATGATCGTCTCCTCCGTCGTGATGGTCGTTTTTGGTGTTGCGACGAACCAGCACGGTTAAGCCCGCCAGCACAGCCAGTGGTAGCATCATGCCTTTATACAGGGTGTGTTGAATATTTTCGGAACGCGCACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCACC  >  NC_000913/3144149‑3144415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑