Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,157,607	T→C	intergenic (+157/‑43)	dkgA → / → yqhG	methylglyoxal reductase DkgA/DUF3828 domain‑containing protein YqhG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,157,607	0	T	C	100.0%	220.0 / NA	60	intergenic (+157/‑43)	dkgA/yqhG	methylglyoxal reductase DkgA/DUF3828 domain‑containing protein YqhG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/25);  total (35/25)

CCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAATATAAGGACATCATCATGCAGAGCCGGAAGCTCTTAAAAGAACAACTCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATA  >  NC_000913/3157487‑3157743                                                                                                                         |                                                                                                                                         ccGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAATATAAGGACATCATCATGCAGAGCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATaaa                                                                                                                       <  2:350160/140‑1 (MQ=255) ccGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAATATAAGGACATCATCATGCAGAGCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATaaa                                                                                                                       <  2:374050/140‑1 (MQ=255) ccGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAATATAAGGACATCATCATGCAGAGCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATaaa                                                                                                                       <  1:410882/140‑1 (MQ=255)                   aTTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAATATAAGGACATCATCATGCAGAGCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGaaa                                                                                                     >  1:822515/1‑140 (MQ=255)                   aTTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAATATAAGGACATCATCATGCAGAGCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGaaa                                                                                                     >  2:295093/1‑140 (MQ=255)                        ttCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAATATAAGGACATCATCATGCAGAGCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAg                                                                                                >  1:881412/1‑140 (MQ=255)                           cccTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAATATAAGGACATCATCATGCAGAGCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATg                                                                                             <  2:577662/140‑1 (MQ=255)                            ccTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAATATAAGGACATCATCATGCAGAGCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGa                                                                                            >  2:40242/1‑140 (MQ=255)                               cgcgTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAATATAAGGACATCATCATGCAGAGCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGaaaa                                                                                         >  1:491523/1‑140 (MQ=255)                                      cgcccgTTGTTACTCTTCCTTGTTCAGGAATGCCAAATATAAGGACATCATCATGCAGAGCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACt                                                                                  >  2:483967/1‑140 (MQ=255)                                        cccgTTGTTACTCTTCCTTGTTCAGGAATGCCAAATATAAGGACATCATCATGCAGAGCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTc                                                                                >  1:155284/1‑140 (MQ=255)                                                       ccTTGTTCAGGAATGCCAAATATAAGGACATCATCATGCAGAGCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGccc                                                                 >  2:918981/1‑140 (MQ=255)                                                          tGTTCAGGAATGCCAAATATAAGGACATCATCATGCAGAGCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTgg                                                              <  1:804556/140‑1 (MQ=255)                                                          tGTTCAGGAATGCCAAATATAAGGACATCATCATGCAGAGCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTgg                                                              >  1:497057/1‑140 (MQ=255)                                                           gTTCAGGAATGCCAAATATAAGGACATCATCATGCAGAGCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGc                                                             <  2:607876/140‑1 (MQ=255)                                                            ttCAGGAATGCCAAATATAAGGACATCATCATGCAGAGCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCa                                                            >  2:662398/1‑140 (MQ=255)                                                             tCAGGAATGCCAAATATAAGGACATCATCATGCAGAGCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCaa                                                           >  2:826246/1‑140 (MQ=255)                                                                ggAATGCCAAATATAAGGACATCATCATGCAGAGCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGtt                                                        <  1:938183/140‑1 (MQ=255)                                                                  aaTGCCAAATATAAGGACATCATCATGCAGAGCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGtttt                                                      <  1:483967/140‑1 (MQ=255)                                                                    tGCCAAATATAAGGACATCATCATGCAGAGCCGGAAGCTCTTACAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTAc                                                    <  2:881412/140‑1 (MQ=255)                                                                    tGCCAAATATAAGGACATCATCATGCAGAGCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTAc                                                    <  2:491523/140‑1 (MQ=255)                                                                      ccAAATATAAGGACATCATCATGCAGAGCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCg                                                  >  2:408242/1‑140 (MQ=255)                                                                      ccAAATATAAGGACATCATCATGCAGAGCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCg                                                  >  1:634373/1‑140 (MQ=255)                                                                      ccAAATATAAGGACATCATCATGCAGAGCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCg                                                  >  2:95350/1‑140 (MQ=255)                                                                       cAAATATAAGGACATCATCATGCAGAGCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGt                                                 <  1:40242/140‑1 (MQ=255)                                                                           tataAGGACATCATCATGCAGAGCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTacac                                             >  1:502034/1‑140 (MQ=255)                                                                                ggACATCATCATGCAGAGCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGcaca                                        >  1:588710/1‑140 (MQ=255)                                                                                ggACATCATCATGCAGAGCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGcaca                                        >  2:131522/1‑140 (MQ=255)                                                                                     tcatcatGCAGAGCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGcaca                                        <  1:770312/135‑1 (MQ=255)                                                                                     tcatcatGCAGAGCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGcaca                                        >  2:770312/1‑135 (MQ=255)                                                                                     tcatcatGCAGAGCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGcccc                                   >  2:243861/1‑140 (MQ=255)                                                                                     tcatcatGCAGAGCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGcccc                                   >  1:803199/1‑140 (MQ=255)                                                                                     tcatcatGCAGAGCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGcccc                                   >  2:411899/1‑140 (MQ=255)                                                                                      catcatGCAGAGCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGccccc                                  >  1:790519/1‑140 (MQ=255)                                                                                       atcatGCAGAGCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGcccccc                                 >  1:130679/1‑140 (MQ=255)                                                                                        tcatGCAGAGCCGGAAGCTCTTAAAAGGACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCt                                >  2:759316/1‑140 (MQ=255)                                                                                            gCAGAGCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGccc                                    >  2:171652/1‑132 (MQ=255)                                                                                            gCAGAGCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGccc                                    <  1:171652/132‑1 (MQ=255)                                                                                            gCAGAGCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTcaca                            <  1:662398/140‑1 (MQ=255)                                                                                              agagCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGa                          <  1:720339/140‑1 (MQ=255)                                                                                                agCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGAcc                        <  2:155284/140‑1 (MQ=255)                                                                                                agCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGAcc                        >  1:696070/1‑140 (MQ=255)                                                                                                agCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGAcc                        >  1:405982/1‑140 (MQ=255)                                                                                                agCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGAcc                        <  1:243861/140‑1 (MQ=255)                                                                                                agCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGAcc                        <  1:131522/140‑1 (MQ=255)                                                                                                 gCCGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCg                       <  1:295093/140‑1 (MQ=255)                                                                                                  ccGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGcaca                                        <  2:239308/122‑1 (MQ=255)                                                                                                  ccGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGcaca                                        >  1:239308/1‑122 (MQ=255)                                                                                                  ccGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCg                       >  2:97910/1‑139 (MQ=255)                                                                                                  ccGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCg                       <  1:97910/139‑1 (MQ=255)                                                                                                   cGGAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTa                     >  1:12329/1‑140 (MQ=255)                                                                                                     gAAGCTCTTAAAAGAACAACGCCTCTATATCCCGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGa                   >  2:5646/1‑140 (MQ=255)                                                                                                     gAAGCTCTTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAGCAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGa                   >  2:186871/1‑140 (MQ=255)                                                                                                          tctTAAAAGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGaacaaa              <  2:588710/140‑1 (MQ=255)                                                                                                                 aGAACAACCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCg       <  2:895000/140‑1 (MQ=255)                                                                                                                   aacaacCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGc     <  2:696070/140‑1 (MQ=255)                                                                                                                   aacaacCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGc     >  2:228220/1‑140 (MQ=255)                                                                                                                   aacaacCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGc     >  1:84753/1‑140 (MQ=255)                                                                                                                     caacCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCat   >  1:267098/1‑140 (MQ=255)                                                                                                                     caacCCATCCATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCat   >  1:383411/1‑140 (MQ=255)                                                                                                                      aacCCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCata  <  2:634373/140‑1 (MQ=255)                                                                                                                         |                                                                                                                                         CCGGACTTTTCAGAAATCATTTATTCCCCTCGCGTCCCGCCCGTTGTTACTCTTCCTTGTTCAGGAATGCCAAATATAAGGACATCATCATGCAGAGCCGGAAGCTCTTAAAAGAACAACTCATCTATATCCGGGATAAACGCAACGGAGAGGTGAAAAACAGATGAAAATAATACTTCTGTTTTTAGCAGCCCTGGCAAGTTTTACCGTACACGCACAGCCCCCCTCACAGACCGTAGAACAAACAGTCCGGCATA  >  NC_000913/3157487‑3157743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑