Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,162,416	T→C	D85G (GAC→GGC)	ftsP ←	cell division protein required during stress conditions

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,162,416	0	T	C	100.0%	139.0 / NA	40	D85G (GAC→GGC)	ftsP	cell division protein required during stress conditions
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/24);  total (16/24)

CGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAG  >  NC_000913/3162285‑3162552                                                                                                                                    |                                                                                                                                         cGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGCCGCCCTTc                                                                                                                                  <  1:482705/140‑1 (MQ=255)    gTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGCCGCCCTTCCAg                                                                                                                               >  1:782645/1‑140 (MQ=255)    gTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGCCGCCCTTCCAg                                                                                                                               >  2:599527/1‑140 (MQ=255)          aGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGCCGCCCTTCCAGACGCgg                                                                                                                         <  2:565054/140‑1 (MQ=255)           gTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGCCGCCCTTCCAGACGCGGa                                                                                                                        <  1:161521/140‑1 (MQ=255)               gCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGCCGCCCTTCCAGACGCGGATAGt                                                                                                                    <  1:488060/140‑1 (MQ=255)                   ttGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGCCGCCCTTCCAGACGCGGATAGTCGGc                                                                                                                <  1:253524/140‑1 (MQ=255)                            tcatcCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGCCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTaa                                                                                                       <  2:708458/140‑1 (MQ=255)                            tcatcCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGCCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTaa                                                                                                       <  1:599527/140‑1 (MQ=255)                                         aCCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGCCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTc                                                                                          >  1:322511/1‑140 (MQ=255)                                             cccATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGCCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCa                                                                                      >  2:381898/1‑140 (MQ=255)                                             cccATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGCCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCa                                                                                      >  1:701058/1‑140 (MQ=255)                                                  cAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGCCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCg                                                                                 <  2:782645/140‑1 (MQ=255)                                                       ggCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGCCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGAcgcg                                                                            >  2:49135/1‑140 (MQ=255)                                                         ccTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGCCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGAcgcgcg                                                                          <  2:322511/140‑1 (MQ=255)                                                                  tGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGCCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTgg                                                                 <  1:4090/140‑1 (MQ=255)                                                                  tGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGCCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTgg                                                                 >  2:916944/1‑140 (MQ=255)                                                                       cccGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGCCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTccc                                                                    <  2:594497/132‑1 (MQ=255)                                                                       cccGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGCCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTccc                                                                    >  1:594497/1‑132 (MQ=255)                                                                       cccGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGCCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTaa                                                            >  1:238408/1‑140 (MQ=255)                                                                        ccGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGCCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTaaa                                                           >  2:499690/1‑140 (MQ=255)                                                                           gCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGCCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGa                                                        >  2:273640/1‑140 (MQ=255)                                                                           gCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGCCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGa                                                        >  1:196279/1‑140 (MQ=255)                                                                           gCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGCCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGa                                                        >  2:787355/1‑140 (MQ=255)                                                                            ccACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGCCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGAc                                                       <  1:273640/140‑1 (MQ=255)                                                                                  tCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGCCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTgc                                                 <  1:916944/140‑1 (MQ=255)                                                                                  tCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGCCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTgc                                                 <  1:787355/140‑1 (MQ=255)                                                                                  tCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGCCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTgc                                                 <  1:381898/140‑1 (MQ=255)                                                                                    aTTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGCCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTgcgc                                               <  2:861473/140‑1 (MQ=255)                                                                                     ttGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGCCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCa                                              <  1:49135/140‑1 (MQ=255)                                                                                        agaCATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGCCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGt                                           <  2:701058/140‑1 (MQ=255)                                                                                            aTTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGCCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTa                                       <  2:196279/140‑1 (MQ=255)                                                                                            aTTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGCCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTa                                       <  1:577974/140‑1 (MQ=255)                                                                                              tttCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGCCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACa                                     <  2:593602/140‑1 (MQ=255)                                                                                                 cTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGCCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTc                                  <  2:238408/140‑1 (MQ=255)                                                                                                        gCGGTTGCTGTAAATAAGCTTAACATCGCCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACa                           >  1:350918/1‑140 (MQ=255)                                                                                                            ttGCTGTAAATAAGCTTAACATCGCCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCgg                       <  2:223289/140‑1 (MQ=255)                                                                                                                         gCTTAACATCGCCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCga          <  2:121470/140‑1 (MQ=255)                                                                                                                         gCTTAACATCGCCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCga          >  2:729180/1‑140 (MQ=255)                                                                                                                              aCATCGCCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTc     >  2:937537/1‑140 (MQ=255)                                                                                                                                 tcgccgCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAg  <  1:729180/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                         CGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCAGCGGGCCTGGTACCTGTAGCCCGGCCACCGTCATTGAGACATTTTCTGTCAGGCGGTTGCTGTAAATAAGCTTAACATCGTCGCCCTTCCAGACGCGGATAGTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAG  >  NC_000913/3162285‑3162552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑