Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,272,432	G→A	A107V (GCG→GTG)	garR ←	tartronate semialdehyde reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,272,432	0	G	A	100.0%	214.4 / NA	61	A107V (GCG→GTG)	garR	tartronate semialdehyde reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/35);  total (26/35)

CGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAGTTTGGCAGCA  >  NC_000913/3272296‑3272564                                                                                                                                         |                                                                                                                                     cGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACtt                                                                                                                                   >  1:444004/1‑140 (MQ=255)            aaTCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCa                                                                                                                        <  2:185059/140‑1 (MQ=255)            aaTCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCa                                                                                                                        <  2:790238/140‑1 (MQ=255)              tCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACg                                                                                                                      >  2:416691/1‑140 (MQ=255)                 tAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCt                                                                                                                   <  2:171655/140‑1 (MQ=255)                     aTTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGcc                                                                                                               <  2:605326/140‑1 (MQ=255)                       ttGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTATCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAg                                                                                                             <  2:412656/140‑1 (MQ=255)                        tGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGc                                                                                                            >  1:934267/1‑140 (MQ=255)                        tGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGc                                                                                                            >  1:624111/1‑140 (MQ=255)                         gTCGAAAATAGCCTTGTCACCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCg                                                                                                           >  1:510918/1‑140 (MQ=255)                           cGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCAGGGCGTGCCAGCGGt                                                                                                         >  2:821486/1‑140 (MQ=255)                            gAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCGTCCAACATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTg                                                                                                        <  2:872237/140‑1 (MQ=255)                                aTAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGAt                                                                                                    <  2:278873/140‑1 (MQ=255)                                aTAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGAt                                                                                                    <  2:386175/140‑1 (MQ=255)                                    ccTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGAt                                                                                                    >  1:688580/1‑136 (MQ=255)                                    ccTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGAt                                                                                                    <  2:688580/136‑1 (MQ=255)                                    ccTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGaa                                                                                                <  2:262378/140‑1 (MQ=255)                                    ccTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGaa                                                                                                >  1:260150/1‑140 (MQ=255)                                        gTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCa                                                                                            <  1:883487/140‑1 (MQ=255)                                        gTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCa                                                                                            <  1:710158/140‑1 (MQ=255)                                             cgccCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCg                                                                                       <  1:647415/140‑1 (MQ=255)                                              gccCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGa                                                                                      >  1:245120/1‑140 (MQ=255)                                              gccCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATAGa                                                                                      >  2:947095/1‑140 (MQ=255)                                               cccACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGAt                                                                                     <  1:335464/140‑1 (MQ=255)                                                 caccaTCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCa                                                                                   <  1:343125/140‑1 (MQ=255)                                                                cGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCt                                                                    >  2:554521/1‑140 (MQ=255)                                                                 gTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATa                                                                                <  2:112497/127‑1 (MQ=255)                                                                 gTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATa                                                                                >  1:112497/1‑127 (MQ=255)                                                                  tACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTc                                                                  >  2:674966/1‑140 (MQ=255)                                                                  tACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTc                                                                  >  1:24002/1‑140 (MQ=255)                                                                   aCCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTcg                                                                 <  1:674966/140‑1 (MQ=255)                                                                    ccGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTAcc                                                                         >  2:728142/1‑131 (MQ=255)                                                                    ccGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTAcc                                                                         <  1:728142/131‑1 (MQ=255)                                                                    ccGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTcgc                                                                <  1:85930/140‑1 (MQ=255)                                                                      gTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCgcgc                                                              >  2:493935/1‑140 (MQ=255)                                                                       tCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGcc                                                             <  2:1597/140‑1 (MQ=255)                                                                       tCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGcc                                                             <  1:726300/140‑1 (MQ=255)                                                                         gATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCtt                                                           <  2:738109/140‑1 (MQ=255)                                                                          aTGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGcc                                                             <  2:796407/137‑1 (MQ=255)                                                                          aTGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGcc                                                             >  1:796407/1‑137 (MQ=255)                                                                          aTGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTc                                                          >  2:921842/1‑140 (MQ=255)                                                                                  cGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGc                                                  <  2:65182/140‑1 (MQ=255)                                                                                  cGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGc                                                  <  1:416691/140‑1 (MQ=255)                                                                                  cGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGc                                                  <  2:444004/140‑1 (MQ=255)                                                                                  cGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGc                                                  <  1:921842/140‑1 (MQ=255)                                                                                        aCCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATtct                                            >  2:465014/1‑140 (MQ=255)                                                                                            ccgcTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCAcc                                        <  1:837150/140‑1 (MQ=255)                                                                                                    cGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCa                                <  2:930342/140‑1 (MQ=255)                                                                                                    cGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCa                                <  2:313120/140‑1 (MQ=255)                                                                                                      gAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCAcc                              <  2:269823/140‑1 (MQ=255)                                                                                                         cATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCAcc                              <  1:82087/137‑1 (MQ=255)                                                                                                         cATCCAGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCAcc                              >  2:82087/1‑137 (MQ=255)                                                                                                              aGCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTcac                      >  2:738406/1‑140 (MQ=255)                                                                                                               gCATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTcaca                     <  1:738406/140‑1 (MQ=255)                                                                                                                cATATCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACAt                    <  2:24002/140‑1 (MQ=255)                                                                                                                  tatCAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGa                  >  1:70517/1‑140 (MQ=255)                                                                                                                     cAATGCCTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAggg               >  2:888574/1‑140 (MQ=255)                                                                                                                          ccTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAGtt          >  1:57596/1‑140 (MQ=255)                                                                                                                          ccTTTCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAGtt          >  2:331703/1‑140 (MQ=255)                                                                                                                              tCGCTTTCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAGTTTGgc      >  2:798703/1‑140 (MQ=255)                                                                                                                                  tttCAGCACTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAGTTTGgcagca  <  2:260150/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     CGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAGTTTGGCAGCA  >  NC_000913/3272296‑3272564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑