Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,368,754 A→G T310A (ACG→GCG)  yhcG → DUF1016 domain‑containing protein YhcG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,368,7540AG100.0% 198.6 / NA 55T310A (ACG→GCG) yhcGDUF1016 domain‑containing protein YhcG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/24);  total (31/24)

TGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAA  >  NC_000913/3368619‑3368890
                                                                                                                                       |                                                                                                                                        
tGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGGCGCt                                                                                                                                      >  1:870036/1‑140 (MQ=255)
  gTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGGCGCTAc                                                                                                                                    >  1:272477/1‑140 (MQ=255)
       gtgtCGATTTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGGCGCTa                                                                                                                                     <  1:906137/134‑1 (MQ=255)
       gtgtCGATTTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGGCGCTa                                                                                                                                     >  2:906137/1‑134 (MQ=255)
       gtgtCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGGCGCTACCGGAt                                                                                                                               >  2:550937/1‑140 (MQ=255)
         gtCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGGCGCTACCGGATGa                                                                                                                             >  1:142610/1‑140 (MQ=255)
                    gTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCa                                                                                                                  >  1:677104/1‑140 (MQ=255)
                     tttttCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCAt                                                                                                                 <  2:446000/140‑1 (MQ=255)
                     tttttCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCAt                                                                                                                 <  2:272477/140‑1 (MQ=255)
                       tttCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCg                                                                                                               <  2:620886/140‑1 (MQ=255)
                            aCCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTg                                                                                                          >  2:731394/1‑140 (MQ=255)
                              cgccgTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGt                                                                                                        <  2:142610/140‑1 (MQ=255)
                                   tttACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTctct                                                                                                   <  2:677104/140‑1 (MQ=255)
                                   tttACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTctct                                                                                                   <  2:507180/140‑1 (MQ=255)
                                           gcctgcTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCagag                                                                                           >  2:336551/1‑140 (MQ=255)
                                           gcctgcTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCagag                                                                                           >  2:125089/1‑140 (MQ=255)
                                              tgcTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGaaa                                                                                        <  1:512940/140‑1 (MQ=255)
                                                  aaTCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAg                                                                                    <  1:125089/140‑1 (MQ=255)
                                                      gtcgATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCgg                                                                                >  1:489807/1‑140 (MQ=255)
                                                        cgATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGgag                                                                              >  2:754055/1‑140 (MQ=255)
                                                           tCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAg                                                                           <  1:550937/140‑1 (MQ=255)
                                                                 aGTGGGCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCAtt                                                                     <  1:133087/140‑1 (MQ=255)
                                                                      gCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGGGAAGCGCATTATGct                                                                >  1:47996/1‑140 (MQ=255)
                                                                      gCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGct                                                                >  2:96638/1‑140 (MQ=255)
                                                                               gCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGt                                                       <  2:489807/140‑1 (MQ=255)
                                                                                cTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGtt                                                      >  1:917037/1‑140 (MQ=255)
                                                                                    aGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGcc                                                  <  1:226627/140‑1 (MQ=255)
                                                                                        aTGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAac                                              >  1:283387/1‑140 (MQ=255)
                                                                                          gCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAacac                                            >  2:896656/1‑140 (MQ=255)
                                                                                               aCAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAaca                                             >  1:79354/1‑134 (MQ=255)
                                                                                               aCAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAaca                                             <  2:79354/134‑1 (MQ=255)
                                                                                                      aaTATGTATCTCAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAg                                <  1:685396/140‑1 (MQ=255)
                                                                                                      aaTATGTATCTCAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAg                                <  2:771600/140‑1 (MQ=255)
                                                                                                        tatGTATCTCAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGc                               >  1:279255/1‑139 (MQ=255)
                                                                                                        tatGTATCTCAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGc                               <  2:279255/139‑1 (MQ=255)
                                                                                                        tatGTATCTCAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGc                               <  2:801702/139‑1 (MQ=255)
                                                                                                        tatGTATCTCAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGc                               >  1:801702/1‑139 (MQ=255)
                                                                                                             aTCTCAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAatat                         >  1:256440/1‑140 (MQ=255)
                                                                                                             aTCTCAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAatat                         >  2:716227/1‑140 (MQ=255)
                                                                                                             aTCTCAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAata                          <  1:305283/139‑1 (MQ=255)
                                                                                                             aTCTCAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAata                          >  2:305283/1‑139 (MQ=255)
                                                                                                              tctcAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAAtata                        <  1:896656/140‑1 (MQ=255)
                                                                                                                 cAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGa                     >  1:186836/1‑140 (MQ=255)
                                                                                                                 cAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGa                     <  2:870036/140‑1 (MQ=255)
                                                                                                                 cAACTACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGa                     <  1:96638/140‑1 (MQ=255)
                                                                                                                     tACGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCa                 <  1:336551/140‑1 (MQ=255)
                                                                                                                      aCGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCTGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCaa                >  2:514864/1‑140 (MQ=255)
                                                                                                                      aCGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCaa                >  2:64561/1‑140 (MQ=255)
                                                                                                                       cGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATGAGATGCAAc               >  1:716774/1‑140 (MQ=255)
                                                                                                                       cGCCAAAGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAAc               >  1:606209/1‑140 (MQ=255)
                                                                                                                            aaGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGa                     >  2:219609/1‑129 (MQ=255)
                                                                                                                            aaGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGa                     <  1:219609/129‑1 (MQ=255)
                                                                                                                            aaGAGCACTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCt          >  1:403853/1‑140 (MQ=255)
                                                                                                                                  aCTGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATgag    >  2:477676/1‑140 (MQ=255)
                                                                                                                                    tGGGCGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGaa  >  1:677271/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                        
TGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGCCAAAGAGCACTGGACGCTACCGGATGAAAATCCGCCCATCGGTCTGGTTCTCTGTGCAGAGAAAGGAGCCGGAGAAGCGCATTATGCTCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAA  >  NC_000913/3368619‑3368890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑