Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,393,494	T→C	N922S (AAC→AGC)	yhdP ←	AsmA2 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,393,494	0	T	C	100.0%	210.2 / NA	58	N922S (AAC→AGC)	yhdP	AsmA2 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/31);  total (27/31)

ATTTGATATTCGCCAGCCACGGCGCGTTATTACGCATCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGTTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATCGGCGG  >  NC_000913/3393378‑3393630                                                                                                                     |                                                                                                                                         atTTGATATTCGCCAGCCACGGCGCGTTATTACGCATCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGt                                                                                                                   >  1:97965/1‑140 (MQ=255) atTTGATATTCGCCAGCCACGGCGCGTTATTACGCATCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGt                                                                                                                   <  1:199288/140‑1 (MQ=255)   ttGATATTCGCCAGCCACGGCGCGTTATTACGCATCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGa                                                                                                                 <  1:707685/140‑1 (MQ=255)      atatTCGCCAGCCACGGCGCGTTATTACGCATCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGTCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGacaa                                                                                                              >  1:811689/1‑140 (MQ=255)      atatTCGCCAGCCACGGCGCGTTATTACGCATCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGacaa                                                                                                              >  1:59471/1‑140 (MQ=255)        atTCGCCAGCCACGGCGCGTTATTACGCATCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGacaaca                                                                                                            <  2:899997/140‑1 (MQ=255)         ttCGCCAGCCACGGCGCGTTATTACGCATCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACAt                                                                                                           >  1:139892/1‑140 (MQ=255)           cGCCAGCCACGGCGCGTTATTACGCATCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAg                                                                                                         <  1:818297/140‑1 (MQ=255)           cGCCAGCCACGGCGCGTTATTACGCATCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAg                                                                                                         <  1:772745/140‑1 (MQ=255)            gccagccaCGGCGCGTTATTACGCATCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAgg                                                                                                        <  1:183840/140‑1 (MQ=255)            gccagccaCGGCGCGTTATTACGCATCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAgg                                                                                                        >  2:471201/1‑140 (MQ=255)             ccagccaCGGCGCGTTATTACGCATCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGc                                                                                                       <  2:59471/140‑1 (MQ=255)                  caCGGCGCGTTATTACGCATCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGcgtacg                                                                                                  <  1:601545/140‑1 (MQ=255)                   aCGGCGCGTTATTACGCATCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGcgtacgt                                                                                                 <  1:121113/140‑1 (MQ=255)                    cGGCGCGTTATTACGCATCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGcgtacgta                                                                                                <  2:139892/140‑1 (MQ=255)                           ttattaCGCATCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTAt                                                                                         <  1:471201/140‑1 (MQ=255)                                   cATCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTtgtg                                                                                 >  2:665565/1‑140 (MQ=255)                                     tCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTggg                                                                               >  1:471700/1‑140 (MQ=255)                                      cGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACg                                                                                             >  1:803592/1‑125 (MQ=255)                                      cGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACg                                                                                             <  2:803592/125‑1 (MQ=255)                                      cGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGa                                                                              >  1:625728/1‑140 (MQ=255)                                      cGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGa                                                                              >  2:546039/1‑140 (MQ=255)                                      cGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGa                                                                              >  2:483896/1‑140 (MQ=255)                                      cGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGa                                                                              >  2:348333/1‑140 (MQ=255)                                        cTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGaaa                                                                            >  2:7651/1‑140 (MQ=255)                                            cGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCg                                                                        <  1:30153/140‑1 (MQ=255)                                             gTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTAt                                                                                         >  1:89139/1‑122 (MQ=255)                                             gTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTAt                                                                                         <  2:89139/122‑1 (MQ=255)                                              tGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCt                                                                      <  1:437045/140‑1 (MQ=255)                                               ggCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTg                                                                     <  2:472806/140‑1 (MQ=255)                                                gCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGTGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGc                                                                    >  1:229795/1‑140 (MQ=255)                                                gCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGc                                                                    >  2:936595/1‑140 (MQ=255)                                                 cGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGca                                                                   >  2:100561/1‑140 (MQ=255)                                                  gTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGcac                                                                  <  2:97965/140‑1 (MQ=255)                                                  gTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGcac                                                                  <  1:259769/140‑1 (MQ=255)                                                   ttGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGcacc                                                                 >  1:640849/1‑140 (MQ=255)                                                    tGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGcacca                                                                <  2:625728/140‑1 (MQ=255)                                                       tttCACGCCCTTGCGCTTCAACCAGGGTGTCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCg                                                             <  2:811689/140‑1 (MQ=255)                                                                  tGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCg                                                             <  1:703951/129‑1 (MQ=255)                                                                  tGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCg                                                             >  2:703951/1‑129 (MQ=255)                                                                       ttCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAgcgc                                             <  2:944502/140‑1 (MQ=255)                                                                       ttCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAgcgc                                             <  2:230865/140‑1 (MQ=255)                                                                         cAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAgcgc                                             >  1:155317/1‑138 (MQ=255)                                                                         cAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAgcgc                                             <  2:155317/138‑1 (MQ=255)                                                                         cAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAGCGCCt                                           <  2:471700/140‑1 (MQ=255)                                                                           aCCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAGCGCCttt                                         >  2:245638/1‑140 (MQ=255)                                                                               gggTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAgcgc                                             >  2:475154/1‑132 (MQ=255)                                                                               gggTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAgcgc                                             <  1:475154/132‑1 (MQ=255)                                                                                 gTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAGCGCCTTTCTGGaa                                   >  1:258749/1‑140 (MQ=255)                                                                                 gTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAGCGCCTTTCTGGaa                                   <  1:546039/140‑1 (MQ=255)                                                                                         tGCCGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAGCGCCTTTCTGGAACAGGgcca                           >  1:871056/1‑140 (MQ=255)                                                                                           ccGTCGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAGCGCCTTTCTGGAACAGGgccagc                         >  2:35706/1‑140 (MQ=255)                                                                                               cGGTTGCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAGCGCCTTTCTGGAACAGGGCCAGCCACt                     <  1:816244/140‑1 (MQ=255)                                                                                                    gCGAAACAATACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCa                <  1:7651/140‑1 (MQ=255)                                                                                                            aTACTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCAt        >  2:13371/1‑140 (MQ=255)                                                                                                              aCTCAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATcg      <  1:665565/140‑1 (MQ=255)                                                                                                                 cAGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGTGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATcggcg   <  2:229795/140‑1 (MQ=255)                                                                                                                  aGGCTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATcggcgg  >  2:384919/1‑140 (MQ=255)                                                                                                                     |                                                                                                                                         ATTTGATATTCGCCAGCCACGGCGCGTTATTACGCATCGCTAGCGTGGCGTTGATTTCACGCCCTTGCGCTTCAACCAGGGTGCCATTTGCCGTCGGTTGCGAAACAATACTCAGGTTATTCCATTGCTGATTTCCCAGTGACAACATAGGCGTACGTAACGTTATGTGTTGTGGGAAACTCGCTGCACCACCGACACTCTCCGCAGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATCGGCGG  >  NC_000913/3393378‑3393630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑