Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,441,981	T→C	N40D (AAC→GAC)	rpsK ←	30S ribosomal subunit protein S11

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,441,981	0	T	C	100.0%	173.9 / NA	49	N40D (AAC→GAC)	rpsK	30S ribosomal subunit protein S11
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/19);  total (30/19)

ATAACTTCCAGATTCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTTACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGAGACTTGTTTTCTTACACGTTTACGTGCACGAATTGGTGCCTTTGCCATTATTCAATCACCCCGAT  >  NC_000913/3441845‑3442115                                                                                                                                         |                                                                                                                                       aTAACTTCCAGATTCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCAcc                                                                                                                                     >  2:470373/1‑140 (MQ=255)   aaCTTCCAGATTCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCt                                                                                                                                   >  2:460327/1‑140 (MQ=255)   aaCTTCCAGATTCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCt                                                                                                                                   >  2:785377/1‑140 (MQ=255)     cTTCCAGATTCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCGCCCTGa                                                                                                                                 <  2:529084/140‑1 (MQ=255)     cTTCCAGATTCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGa                                                                                                                                 >  2:134955/1‑140 (MQ=255)     cTTCCAGATTCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGa                                                                                                                                 <  2:799389/140‑1 (MQ=255)       tCCAGATTCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACg                                                                                                                               <  1:470373/140‑1 (MQ=255)        ccAGATTCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGa                                                                                                                              >  2:689887/1‑140 (MQ=255)         cAGATTCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGAt                                                                                                                             <  1:681819/140‑1 (MQ=255)         cAGATTCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGAt                                                                                                                             >  2:478933/1‑140 (MQ=255)              tCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTg                                                                                                                        <  1:686385/140‑1 (MQ=255)                ttGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGAt                                                                                                                      <  1:134955/140‑1 (MQ=255)                   aTGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGt                                                                                                                   <  1:568096/140‑1 (MQ=255)                      ccGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCAc                                                                                                                >  2:95930/1‑140 (MQ=255)                        gTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGa                                                                                                              >  2:803407/1‑140 (MQ=255)                          aTTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATg                                                                                                            <  1:478933/140‑1 (MQ=255)                          aTTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATg                                                                                                            <  1:95930/140‑1 (MQ=255)                             cTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGgtg                                                                                                         <  1:689887/140‑1 (MQ=255)                                   cGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGttgttg                                                                                                   >  2:646404/1‑140 (MQ=255)                                   cGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGttgttg                                                                                                   >  2:884034/1‑140 (MQ=255)                                   cGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGttgttg                                                                                                   >  2:256969/1‑140 (MQ=255)                                    ggCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGa                                                                                                  >  1:16520/1‑140 (MQ=255)                                     gCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGaa                                                                                                 >  1:907060/1‑140 (MQ=255)                                      cGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGaaa                                                                                                >  1:136814/1‑140 (MQ=255)                                        tCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAaaga                                                                                              >  1:247796/1‑140 (MQ=255)                                        tCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAaaga                                                                                              >  1:462072/1‑140 (MQ=255)                                            cgcAACGCTCAGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCa                                                                                          <  1:884034/140‑1 (MQ=255)                                                aCGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGa                                                                                      >  2:811578/1‑140 (MQ=255)                                                   ctctGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGatat                                                                                   <  2:427379/140‑1 (MQ=255)                                                          gcAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCAc                                                                            <  2:907060/140‑1 (MQ=255)                                                           cAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACg                                                                           <  1:460327/140‑1 (MQ=255)                                                                  aGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCag                                                                    >  1:824142/1‑140 (MQ=255)                                                                   gCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCaga                                                                   >  1:731976/1‑140 (MQ=255)                                                                    cTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCagag                                                                  <  2:731976/140‑1 (MQ=255)                                                                       cAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGAGACt                                                               >  2:402981/1‑140 (MQ=255)                                                                       cAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGAGACt                                                               >  2:299142/1‑140 (MQ=255)                                                                                        cGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGAGACTTGTTTTCTTACACGttt                                              >  1:790902/1‑140 (MQ=255)                                                                                             aCCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGAGACTTGTTTTCTTACACGTTTACGTg                                         >  2:789601/1‑140 (MQ=255)                                                                                                        cGGAACCACCGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGAGACTTGTTTTCTTACACGTTTACGTGCACGAATTGGt                              >  2:913655/1‑140 (MQ=255)                                                                                                            accaccGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGAGACTTGTTTTCTTACACGTTTACGTGCACGAATTGGTGCCt                          >  2:278947/1‑140 (MQ=255)                                                                                                             ccaccGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGAGACTTGTTTTCTTACACGTTTACGTGCACGAATTGGTGCCtt                         <  1:811578/140‑1 (MQ=255)                                                                                                                 cGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGAGACTTGTTTTCTTACACGTTTACGTGCACGAATTGGTGCCTTTGcc                     <  1:803407/140‑1 (MQ=255)                                                                                                                 cGGCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGAGACTTGTTTTCTTACACGTTTACGTGCACGAATTGGTGCCTTTGcc                     <  2:462072/140‑1 (MQ=255)                                                                                                                  ggCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGAGACTTGTTTTCTTACACGTTTACGTGCACGAATTGGTGCCTTTGCCa                    >  1:711806/1‑140 (MQ=255)                                                                                                                  ggCTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGAGACTTGTTTTCTTACACGTTTACGTGCACGAATTGGTGCCTTTGCCa                    >  1:488872/1‑140 (MQ=255)                                                                                                                    cTGTTGCCCAACCCAACGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGAGACTTGTTTTCTTACACGTTTACGTGCACGAATTGGTGCCTTTGCCatt                  >  2:565156/1‑140 (MQ=255)                                                                                                                              acccaacGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGAGACTTGTTTTCTTACACGTTTACGTGCACGAATTGGTGCCTTTGCCATTATTCAATCAc        >  2:87388/1‑140 (MQ=255)                                                                                                                               cccaacGCGTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGAGACTTGTTTTCTTACACGTTTACGTGCACGAATTGGTGCCTTTGCCATTATTCAATCAcc       <  1:913655/140‑1 (MQ=255)                                                                                                                                    cgcgTCACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGGTATGAGCCACGCCGTCAGAGACTTGTTTTCTTACACGTTTACGTGCACGAATTGGTGCCTTTGCCATTATTCAATCACCCCGat  >  2:467174/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       ATAACTTCCAGATTCTTGATGCCGTATTCTTTCACGGCGTCAGCGCAACGCTCTGCTGCAACCTGAGCTGCAAACGGAGTGGATTTGCGAGAACCACGGAAACCGGAACCACCGGCTGTTGCCCAACCCAACGCGTTACCCTGACGATCAGTGATAGTCACGATGGTGTTGTTGAAAGAAGCATGGATATGAGCCACGCCGTCAGAGACTTGTTTTCTTACACGTTTACGTGCACGAATTGGTGCCTTTGCCATTATTCAATCACCCCGAT  >  NC_000913/3441845‑3442115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑