Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,460,545	C→T	H243H (CAC→CAT)	gspF →	Type II secretion system protein GspF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,460,545	0	C	T	100.0%	248.8 / NA	69	H243H (CAC→CAT)	gspF	Type II secretion system protein GspF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (40/29);  total (40/29)

CAACTGCCGCTGAGTACACGCATTCTTTTAGGTCTGAGCGACACGTTGCAACGTACCGGCCCGACATTATTAGCGACAGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCACCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTC  >  NC_000913/3460411‑3460679                                                                                                                                       |                                                                                                                                       caACTGCCGCTGAGTACACGCATTCTTTTAGGTCTGAGCGACACGTTGCAACGTACCGGCCCGACATTATTAGCGACAGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGttt                                                                                                                                   >  2:394933/1‑140 (MQ=255)           tGAGTACACGCATTCTTTTAGGTCTGAGCGACACGTTGCAACGTACCGGCCCGACATTATTAGCGACAGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATg                                                                                                                         <  2:271624/140‑1 (MQ=255)               tACACGCATTCTTTTAGGTCTGAGCGACACGTTGCAACGTACCGGCCCGACATTATTAGCGACAGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTtgc                                                                                                                     >  2:679409/1‑140 (MQ=255)               tACACGCATTCTTTTAGGTCTGAGCGACACGTTGCAACGTACCGGCCCGACATTATTAGCGACAGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTtgc                                                                                                                     >  1:749932/1‑140 (MQ=255)                  acGCATTCTTTTAGGTCTGAGCGACACGTTGCAACGTACCGGCCCGACATTATTAGCGACAGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTtgctgc                                                                                                                  >  1:314019/1‑140 (MQ=255)                                   tGAGCGACACGTTGCAACGTACCGGCCCGACATTATTAGCGACAGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGc                                                                                                 >  1:230038/1‑140 (MQ=255)                                   tGAGCGACACGTTGCAACGTACCGGCCCGACATTATTAGCGACAGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGc                                                                                                 >  1:537890/1‑140 (MQ=255)                                    gAGCGACACGTTGCAACGTACCGGCCCGACATTATTAGCGACAGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGcc                                                                                                >  2:754064/1‑140 (MQ=255)                                      gCGACACGTTGCAACGTACCGGCCCGACATTATTAGCGACAGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCg                                                                                              <  2:537890/140‑1 (MQ=255)                                          cacGTTGCAACGTACCGGCCCGACATTATTAGCGACAGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGa                                                                                          >  2:506271/1‑140 (MQ=255)                                          cacGTTGCAACGTACCGGCCCGACATTATTAGCGACAGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGa                                                                                          >  2:121746/1‑140 (MQ=255)                                              ttGCAACGTACCGGCCCGACATTATTAGCGACAGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTg                                                                                      <  2:230038/140‑1 (MQ=255)                                               tGCAACGTACCGGCCCGACATTATTAGCGACAGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTgc                                                                                     >  2:819929/1‑140 (MQ=255)                                               tGCAACGTACCGGCCCGACATTATTAGCGACAGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTgc                                                                                     >  2:553847/1‑140 (MQ=255)                                               tGCAACGTACCGGCCCGACATTATTAGCGACAGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTgc                                                                                     >  2:716745/1‑140 (MQ=255)                                                gCAACGTACCGGCCCGACATTATTAGCGACAGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTgcg                                                                                    <  1:394933/140‑1 (MQ=255)                                                 cAACGTACCGGCCCGACATTATTAGCGACAGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTgcgc                                                                                   >  2:699835/1‑140 (MQ=255)                                                     gTACCGGCCCGACATTATTAGCGACAGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCAtt                                                                               <  1:716745/140‑1 (MQ=255)                                                       aCCGGCCCGACATTATTAGCGACAGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTaa                                                                             >  1:499750/1‑140 (MQ=255)                                                       aCCGGCCCGACATTATTAGCGACAGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTaa                                                                             >  2:532012/1‑140 (MQ=255)                                                        ccGGCCCGACATTATTAGCGACAGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAAc                                                                            >  1:604249/1‑140 (MQ=255)                                                         cGGCCCGACATTATTAGCGACAGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTgc                                                                                     <  2:301171/130‑1 (MQ=255)                                                         cGGCCCGACATTATTAGCGACAGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTgc                                                                                     >  1:301171/1‑130 (MQ=255)                                                          ggCCCGACATTATTAGCGACAGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAg                                                                          <  1:553847/140‑1 (MQ=255)                                                          ggCCCGACATTATTAGCGACAGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAg                                                                          <  2:604249/140‑1 (MQ=255)                                                            cccGACATTATTAGCGACAGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAgcg                                                                        <  2:749932/140‑1 (MQ=255)                                                                aCATTATTAGCGACAGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTgc                                                                                     <  2:690900/123‑1 (MQ=255)                                                                aCATTATTAGCGACAGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTgc                                                                                     >  1:690900/1‑123 (MQ=255)                                                                         gCGACAGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCg                                                           <  2:314019/140‑1 (MQ=255)                                                                          cGACAGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGc                                                          >  2:278918/1‑140 (MQ=255)                                                                          cGACAGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGc                                                          <  1:248541/140‑1 (MQ=255)                                                                          cGACAGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGc                                                          >  2:799673/1‑140 (MQ=255)                                                                            aCAGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTAtc                                                               >  2:399929/1‑133 (MQ=255)                                                                            aCAGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTAtc                                                               <  1:399929/133‑1 (MQ=255)                                                                              aGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACtt                                                      >  2:181948/1‑140 (MQ=255)                                                                              aGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACtt                                                      <  1:679409/140‑1 (MQ=255)                                                                                tgtTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCg                                                           <  1:791993/133‑1 (MQ=255)                                                                                tgtTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCg                                                           >  2:791993/1‑133 (MQ=255)                                                                                  tttATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCg                                                           <  1:372726/131‑1 (MQ=255)                                                                                  tttATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCg                                                           >  2:372726/1‑131 (MQ=255)                                                                                    tATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTa                                                <  1:819929/140‑1 (MQ=255)                                                                                    tATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTa                                                >  2:390091/1‑140 (MQ=255)                                                                                          cGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTcc                                                            <  1:514937/122‑1 (MQ=255)                                                                                          cGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTcc                                                            >  2:514937/1‑122 (MQ=255)                                                                                          cGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTg                                           <  1:190733/139‑1 (MQ=255)                                                                                          cGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTg                                           >  2:190733/1‑139 (MQ=255)                                                                                          cGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGc                                          >  1:808063/1‑140 (MQ=255)                                                                                          cGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGc                                          >  1:743611/1‑140 (MQ=255)                                                                                            cTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCaa                                        >  2:753754/1‑140 (MQ=255)                                                                                            cTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCaa                                        >  2:821371/1‑140 (MQ=255)                                                                                            cTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCaa                                        >  1:148668/1‑140 (MQ=255)                                                                                               tAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATcc                                     <  2:241682/140‑1 (MQ=255)                                                                                               tAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATcc                                     <  2:657962/140‑1 (MQ=255)                                                                                                       tGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGt                             >  1:126822/1‑140 (MQ=255)                                                                                                       tGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGt                             >  2:642661/1‑140 (MQ=255)                                                                                                       tGGCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGt                             >  1:459429/1‑140 (MQ=255)                                                                                                         gCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTcc                           <  1:390091/140‑1 (MQ=255)                                                                                                         gCTCTGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTcc                           <  1:821371/140‑1 (MQ=255)                                                                                                            ctGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTcc                           >  1:112380/1‑137 (MQ=255)                                                                                                            ctGGTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTcc                           <  2:112380/137‑1 (MQ=255)                                                                                                              ggTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTctg                      >  1:526064/1‑140 (MQ=255)                                                                                                              ggTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTctg                      <  1:799673/140‑1 (MQ=255)                                                                                                               gTTAAAACGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTctgc                     >  2:122032/1‑140 (MQ=255)                                                                                                                     aCGCGGCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTggatg               <  1:532012/140‑1 (MQ=255)                                                                                                                         ggCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTggatgggat           >  1:646680/1‑140 (MQ=255)                                                                                                                         ggCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTggatggga            >  1:148814/1‑139 (MQ=255)                                                                                                                         ggCAATAACCGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTggatggga            <  2:148814/139‑1 (MQ=255)                                                                                                                                 ccGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGt   <  2:459429/140‑1 (MQ=255)                                                                                                                                  cGCCATCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTc  <  1:181948/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       CAACTGCCGCTGAGTACACGCATTCTTTTAGGTCTGAGCGACACGTTGCAACGTACCGGCCCGACATTATTAGCGACAGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAATAACCGCCACCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTC  >  NC_000913/3460411‑3460679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑