Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,507,545	T→C	N53D (AAT→GAT)	yhfU ←	DUF2620 domain‑containing protein YhfU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,507,545	0	T	C	100.0%	156.5 / NA	45	N53D (AAT→GAT)	yhfU	DUF2620 domain‑containing protein YhfU
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/20);  total (25/20)

AAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCGTTGTGAATGAAAACTTCAAAACAGCCAGGAGCCGTCGCTTCAATAGTTTTTTTAATCTGCTCACGCTGTAAG  >  NC_000913/3507408‑3507678                                                                                                                                          |                                                                                                                                      aaaCGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATCac                                                                                                                                     >  1:582288/1‑140 (MQ=255) aaaCGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATCac                                                                                                                                     >  1:350582/1‑140 (MQ=255)  aaCGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTGTAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATcaca                                                                                                                                    <  1:676829/140‑1 (MQ=255)       cACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATCACACgcgc                                                                                                                               <  1:440968/140‑1 (MQ=255)       cACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATCACACgcgc                                                                                                                               <  1:436753/140‑1 (MQ=255)        aCTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATCACACGCGcc                                                                                                                              >  1:792094/1‑140 (MQ=255)        aCTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATCACACGCGcc                                                                                                                              >  2:863079/1‑140 (MQ=255)            ttCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATCACACGCGCCGa                                                                                                                            <  2:827015/138‑1 (MQ=255)            ttCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATCACACGCGCCGa                                                                                                                            >  1:827015/1‑138 (MQ=255)                     cGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATCACACGCGCCGATGTAATAATcc                                                                                                                 <  1:528043/140‑1 (MQ=255)                       aTCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATCACACGCGCCGATGTAATAATCCAg                                                                                                               >  1:305655/1‑140 (MQ=255)                            tttGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATCACACGCGCCGATGTAATAATCCAGTTGcc                                                                                                          <  2:350582/140‑1 (MQ=255)                            tttGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATCACACGCGCCGATGTAATAATCCAGTTGcc                                                                                                          <  1:501197/140‑1 (MQ=255)                            tttGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATCACACGCGCCGATGTAATAATCCAGTTGcc                                                                                                          <  1:534093/140‑1 (MQ=255)                                 cGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATCACACGCGCCGATGTAATAATCCAGTTGCCCGGAt                                                                                                     <  1:504268/140‑1 (MQ=255)                                                tCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATCACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGcc                                                                                      >  2:353486/1‑140 (MQ=255)                                                tCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATCACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGcc                                                                                      <  2:582288/140‑1 (MQ=255)                                                  gCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATCACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGc                                                                                    <  1:397663/140‑1 (MQ=255)                                                              ggTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATCACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCg                                                                          <  2:143825/138‑1 (MQ=255)                                                              ggTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATCACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCg                                                                          >  1:143825/1‑138 (MQ=255)                                                              ggTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATCACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCGtt                                                                        >  1:610953/1‑140 (MQ=255)                                                                tttGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATCACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCGTtgt                                                                      >  2:446014/1‑140 (MQ=255)                                                                    gCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATCACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCGTTGtgaat                                                                  >  1:281824/1‑140 (MQ=255)                                                                        tGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATCACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCGTTGTGAATGaaa                                                              >  2:524283/1‑140 (MQ=255)                                                                          gTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATCACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCGTTGTGAATGAAAAc                                                            >  1:825787/1‑140 (MQ=255)                                                                          gTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATCACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCGTTGTGAATGAAAAc                                                            >  1:607151/1‑140 (MQ=255)                                                                          gTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATCACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCGTTGTGAATGAAAAc                                                            <  1:446014/140‑1 (MQ=255)                                                                                    tGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATCACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCGTTGTGAATGAAAACTTCAAAACAg                                                  >  2:489683/1‑140 (MQ=255)                                                                                               aTCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATCACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCGTTGTGAATGAAAACTTCAAAACAGCCAGGAGCCGt                                       <  2:610953/140‑1 (MQ=255)                                                                                                tCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATCACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCGTTGTGAATGAAAACTTCAAAACAGCCAGGAGCCGTc                                      >  2:502626/1‑140 (MQ=255)                                                                                                     gcgATGGCAATCGACAATGCCGCGCCCGCACCGGTATCACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCGTTGTGAATGAAAACTTCAAAACAGCCAGGAGCCGTCGCTTc                                 >  1:324008/1‑140 (MQ=255)                                                                                                      cgATGGCAATCGACAATGCCGCGCCCGCACCGGTATCACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCGTTGTGAATGAAAACTTCAAAACAGCCAGGAGCCGTCGCTTCa                                >  2:299055/1‑140 (MQ=255)                                                                                                            cAATCGACAATGCCGCGCCCGCACCGGTATCACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCGTTGTGAATGAAAACTTCAAAACAGCCAGGAGCCGTCGCTTCAATAGtt                          >  2:318073/1‑140 (MQ=255)                                                                                                                       gCCGCGCCCGCACCGGTATCACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCGTTGTGAATGAAAACTTCAAAACAGCCAGGAGCCGTCGCTTCAATAGTTTTTTTAATCTg               >  2:476555/1‑140 (MQ=255)                                                                                                                         cgcgCCCGCACCGGTATCACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCGTTGTGAATGAAAACTTCAAAACAGCCAGGAGCCGTCGCTTCAATAGTTTTTTTAATCTGCt             <  1:176146/140‑1 (MQ=255)                                                                                                                         cgcgCCCGCACCGGTATCACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCGTTGTGAATGAAAACTTCAAAACAGCCAGGAGCCGTCGCTTCAATAGTTTTTTTAATCTGCt             >  1:86168/1‑140 (MQ=255)                                                                                                                           cgcCCGCACCGGTATCACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCGTTGTGAATGAAAACTTCAAAACAGCCAGGAGCCGTCGCTTCAATAg                            <  1:640363/123‑1 (MQ=255)                                                                                                                           cgcCCGCACCGGTATCACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCGTTGTGAATGAAAACTTCAAAACAGCCAGGAGCCGTCGCTTCAATAGTTTTTTTAATCTGCTCa           <  2:324008/140‑1 (MQ=255)                                                                                                                           cgcCCGCACCGGTATCACACGCGCCGATGCAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCGTTGTGAATGAAAACTTCAAAACAGCCAGGAGCCGTCGCTTCAATAg                            >  2:640363/1‑123 (MQ=255)                                                                                                                             cccGCACCGGTATCACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCGTTGTGAATGAAAACTTCAAAACAGCCAGGAGCCGTCGCTTCAATAGTTTTTTTAATCTg               >  2:434889/1‑134 (MQ=255)                                                                                                                             cccGCACCGGTATCACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCGTTGTGAATGAAAACTTCAAAACAGCCAGGAGCCGTCGCTTCAATAGTTTTTTTAATCTg               <  1:434889/134‑1 (MQ=255)                                                                                                                             cccGCACCGGTATCACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCGTTGTGAATGAAAACTTCAAAACAGCCAGGAGCCGTCGCTTCAATAGTTTTTTTAATCTGCTCACg         >  2:92118/1‑140 (MQ=255)                                                                                                                                 cACCGGTATCACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCGTTGTGAATGAAAACTTCAAAACAGCCAGGAGCCGTCGCTTCAATAGTTTTTTTAATCTGCTCACGCTGt     <  1:92118/140‑1 (MQ=255)                                                                                                                                  aCCGGTATCACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCGTTGTGAATGAAAACTTCAAAACAGCCAGGAGCCGTCGCTTCAATAGTTTTTTTAATCTGCTCACGCTGTa    >  2:573430/1‑140 (MQ=255)                                                                                                                                    cGGTATCACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCGTTGTGAATGAAAACTTCAAAACAGCCAGGAGCCGTCGCTTCAATAGTTTTTTTAATCTGCTCACGCTGTAAg  <  2:281824/140‑1 (MQ=255)                                                                                                                                    cGGTATCACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCGTTGTGAATGAAAACTTCAAAACAGCCAGGAGCCGTCGCTTCAATAGTTTTTTTAATCTGCTCACGCTGTAAg  <  1:353486/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      AAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGTAATAATCCAGTTGCCCGGATTTCACCTTCATTGCCGCTTCCATGTCGTTGTGAATGAAAACTTCAAAACAGCCAGGAGCCGTCGCTTCAATAGTTTTTTTAATCTGCTCACGCTGTAAG  >  NC_000913/3507408‑3507678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑