Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,526,328	T→C	intergenic (‑179/‑141)	nudE ← / → igaA	ADP‑sugar diphosphatase NudE/inner membrane protein ‑ inhibits the Rcs signaling pathway

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,526,328	0	T	C	100.0%	260.8 / NA	71	intergenic (‑179/‑141)	nudE/igaA	ADP‑sugar diphosphatase NudE/inner membrane protein ‑ inhibits the Rcs signaling pathway
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/35);  total (36/35)

CGGACACGCTTTCGGCAATGTGAATTGCATGTTATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGTAAGATGGG  >  NC_000913/3526192‑3526462                                                                                                                                         |                                                                                                                                       cGGACACGCTTTCGGCAATGTGAATTGCATGTTATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTg                                                                                                                                     <  1:831045/140‑1 (MQ=255)   gACACGCTTTCGGCAATGTGAATTGCATGTTATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCt                                                                                                                                   <  1:63902/140‑1 (MQ=255)   gACACGCTTTCGGCAATGTGAATTGCATGTTATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCt                                                                                                                                   <  2:757222/140‑1 (MQ=255)        gCTTTCGGCAATGTGAATTGCATGTTATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGAt                                                                                                                              >  2:189003/1‑140 (MQ=255)         cTTTCGGCAATGTGAATTGCATGTTATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATc                                                                                                                             <  2:613094/140‑1 (MQ=255)         cTTTCGGCAATGTGAATTGCATGTTATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATc                                                                                                                             >  1:645265/1‑140 (MQ=255)         cTTTCGGCAATGTGAATTGCATGTTATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATc                                                                                                                             <  1:442865/140‑1 (MQ=255)          tttCGGCAATGTGAATTGCATGTTATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCa                                                                                                                            >  1:819059/1‑140 (MQ=255)          tttCGGCAATGTGAATTGCATGTTATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCa                                                                                                                            >  1:568063/1‑140 (MQ=255)          tttCGGCAATGTGAATTGCATGTTATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCa                                                                                                                            >  1:873750/1‑140 (MQ=255)           ttCGGCAATGTGAATTGCATGTTATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAg                                                                                                                           <  2:818165/140‑1 (MQ=255)            tCGGCAATGTGAATTGCATGTTATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGc                                                                                                                          <  2:730221/140‑1 (MQ=255)            tCGGCAATGTGAATTGCATGTTATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGc                                                                                                                          >  2:521560/1‑140 (MQ=255)                   tgtgAATTGCATGTTATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCgg                                                                                                                        <  1:309657/135‑1 (MQ=255)                   tgtgAATTGCATGTTATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCgg                                                                                                                        >  2:309657/1‑135 (MQ=255)                   tgtgAATTGCATGTTATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACt                                                                                                                   <  2:426302/140‑1 (MQ=255)                      gAATTGCATGTTATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACtgtg                                                                                                                <  2:924662/140‑1 (MQ=255)                        aTTGCATGTTATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGAt                                                                                                              <  1:524592/140‑1 (MQ=255)                        aTTGCATGTTATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGAt                                                                                                              <  1:628657/140‑1 (MQ=255)                        aTTGCATGTTATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGAt                                                                                                              <  1:84870/140‑1 (MQ=255)                                 tATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACt                                                                                                                   >  2:197035/1‑126 (MQ=255)                                 tATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACt                                                                                                                   <  1:197035/126‑1 (MQ=255)                                             tGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAg                                                                                           <  2:766567/138‑1 (MQ=255)                                             tGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAg                                                                                           >  1:766567/1‑138 (MQ=255)                                                 aCTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAAc                                                                                     >  1:528940/1‑140 (MQ=255)                                                     aataaataATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTcga                                                                                 >  2:878870/1‑138 (MQ=255)                                                     aataaataATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCa                                                                                 <  1:878870/140‑1 (MQ=255)                                                      ataaataaTCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCAc                                                                                >  1:460446/1‑140 (MQ=255)                                                            aatCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGc                                                                          <  2:681823/140‑1 (MQ=255)                                                            aatCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGc                                                                          >  1:162628/1‑140 (MQ=255)                                                             atCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGcc                                                                         >  1:147778/1‑140 (MQ=255)                                                                 tCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATa                                                                     >  2:43025/1‑140 (MQ=255)                                                                   cTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGc                                                                   >  2:916758/1‑140 (MQ=255)                                                                    tcaaatcaaaTTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGcc                                                                  <  2:162628/140‑1 (MQ=255)                                                                        atcaaatTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAAcc                                                              >  1:577192/1‑140 (MQ=255)                                                                        atcaaatTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAAcc                                                              >  1:947984/1‑140 (MQ=255)                                                                         tcaaatTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCg                                                             <  1:540507/140‑1 (MQ=255)                                                                           aaatTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCa                                                           <  2:577192/140‑1 (MQ=255)                                                                             atTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGa                                                         <  2:947984/140‑1 (MQ=255)                                                                             atTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGa                                                         <  2:568063/140‑1 (MQ=255)                                                                             atTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGa                                                         >  1:439703/1‑140 (MQ=255)                                                                                       aaTAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGGATCATGTAtt                                               >  1:650697/1‑140 (MQ=255)                                                                                       aaTAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTAtt                                               >  1:246420/1‑140 (MQ=255)                                                                                              tGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCg                                        >  2:335653/1‑140 (MQ=255)                                                                                              tGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGAGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCg                                        >  1:127196/1‑140 (MQ=255)                                                                                               gAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCgg                                       >  2:536569/1‑140 (MQ=255)                                                                                                 aaTAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTg                                     <  1:536569/140‑1 (MQ=255)                                                                                                      cTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACt                                >  1:352499/1‑140 (MQ=255)                                                                                                      cTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACt                                >  2:657556/1‑140 (MQ=255)                                                                                                      cTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACt                                >  2:507880/1‑140 (MQ=255)                                                                                                       tCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTg                               >  1:466496/1‑140 (MQ=255)                                                                                                       tCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTg                               >  2:218482/1‑140 (MQ=255)                                                                                                       tCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTg                               >  2:473309/1‑140 (MQ=255)                                                                                                        ccAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGa                              <  2:460446/140‑1 (MQ=255)                                                                                                        ccAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGa                              <  1:657556/140‑1 (MQ=255)                                                                                                        ccAGGAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGAGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGa                              <  2:127196/140‑1 (MQ=255)                                                                                                            gAATTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCAc                          >  1:775090/1‑140 (MQ=255)                                                                                                             aaTTTGCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACg                         <  1:522348/140‑1 (MQ=255)                                                                                                                  gCTGATATTCCGCCTTCAGAGGGCTTGCTGTGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAAGCATGTATTGTGTCCGGTGCGACTGACCACGCCTgg                    <  1:218482/140‑2 (MQ=255)                                                                                                                  gCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGa                    <  2:147778/140‑1 (MQ=255)                                                                                                                  gCTGATATTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGa                    <  2:352499/140‑1 (MQ=255)                                                                                                                       tatTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGAc               >  1:612944/1‑140 (MQ=255)                                                                                                                        atTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGAc                   <  2:63250/135‑1 (MQ=255)                                                                                                                        atTCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGAc                   >  1:63250/1‑135 (MQ=255)                                                                                                                         ttCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACta             >  2:464406/1‑140 (MQ=255)                                                                                                                          tCCGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACtaa            <  2:246420/140‑1 (MQ=255)                                                                                                                            cGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACtaagt          <  1:507880/140‑1 (MQ=255)                                                                                                                            cGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACtaagt          <  1:335653/140‑1 (MQ=255)                                                                                                                            cGCCTTCAGAGGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACtaagt          <  1:521560/140‑1 (MQ=255)                                                                                                                                   agagGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGTAAGATgg   >  1:758197/1‑140 (MQ=255)                                                                                                                                    gagGGCTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGTAAGATggg  >  1:637655/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       CGGACACGCTTTCGGCAATGTGAATTGCATGTTATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGTAAGATGGG  >  NC_000913/3526192‑3526462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑