Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,605,674	T→C	intergenic (‑64/‑77)	yhhM ← / → yhhN	DUF2500 domain‑containing protein YhhM/conserved inner membrane enzyme YhhN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,605,674	0	T	C	100.0%	123.4 / NA	35	intergenic (‑64/‑77)	yhhM/yhhN	DUF2500 domain‑containing protein YhhM/conserved inner membrane enzyme YhhN
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/14);  total (21/14)

CGAAACGATGCGGCGACGACAATTAAGCCAATGATAACAATAAAGAAAAGAGGTGGTTTGCTCATCTTTATCCCTCATCGGAAAATGCGGAAATAAGCATACCCTGCCAGTTATGGTGTTGTCATCCGTCCACCCTCGCCACTAAACTGGAAGCAAGACCGTAGGCATTCCGCTTACGAAAAAATAACGAATTCAAGGAACTAAGATGCTTTGGTCGTTTATCGCTGTCTGTCTTTCCGCATGGCTATCTGTGGATG  >  NC_000913/3605546‑3605802                                                                                                                                 |                                                                                                                                 cgaaacgaTGCGGCGACGACAATTAAGCCAATGATAACAATAAAGAAAAGAGGTGGTTTGCTCATCTTTATCCCTCATCGGAAAATGCGGAAATAAGCATACCCTGCCAGTTATGGTGTTGTCATCCGCCCACCCTCGcc                                                                                                                       >  1:542951/1‑140 (MQ=255) cgaaacgaTGCGGCGACGACAATTAAGCCAATGATAACAATAAAGAAAAGAGGTGGTTTGCTCATCTTTATCCCTCATCGGAAAATGCGGAAATAAGCATACCCTGCCAGTTATGGTGTTGTCATCCGCCCACCCTCGcc                                                                                                                       >  1:337515/1‑140 (MQ=255)         tGCGGCGACGACAATTAAGCCAACGATAACAATAAAGAAAAGAGGTGGTTTGCTCATCTTTATCCCTCATCGGAAAATGCGGAAATAAGCATACCCTGCCAGTTATGGTGTTGTCATCCGCCCACCCTCGCCACTAAACt                                                                                                               <  2:518984/140‑1 (MQ=255)              cgacgaCAATTAAGCCAATGACAACAATAAAGAAAAGAGGTGGTTTGCTCATCTTTATCCCTCATCGGAAAATGCGGAAATAAGCATACCCTGCCAGTTATGGTGTTGTCATCCGCCCACCCTCGCCACTAAACTGGAAg                                                                                                          <  2:864172/140‑1 (MQ=255)                          aGCCAATGATAACAATAAAGAAAAGAGGTGGTTTGCTCATCTTTATCCCTCATCGGAAAATGCGGAAATAAGCATACCCTGCCAGTTATGGTGTTGTCATCCGCCCACCCTCGCCACTAAACTGGAAGCa                                                                                                        >  2:502376/1‑130 (MQ=255)                          aGCCAATGATAACAATAAAGAAAAGAGGTGGTTTGCTCATCTTTATCCCTCATCGGAAAATGCGGAAATAAGCATACCCTGCCAGTTATGGTGTTGTCATCCGCCCACCCTCGCCACTAAACTGGAAGCa                                                                                                        <  1:502376/130‑1 (MQ=255)                                                 agagGTGGTTTGCTCATCTTTATCCCTCATCGGAAAATGCGGAAATAAGCATACCCTGCCAGTTATGGTGTTGTCATCCGCCCACCCTCGCCACTAAACTGGAAGCAAGACCGTAGGCATTCCGCTTACGAAAAAATAAc                                                                       >  1:809836/1‑140 (MQ=255)                                                 agagGTGGTTTGCTCATCTTTATCCCTCATCGGAAAATGCGGAAATAAGCATACCCTGCCAGTTATGGTGTTGTCATCCGCCCACCCTCGCCACTAAACTGGAAGCAAGACCGTAGGCATTCCGCTTACGAAAAAATAAc                                                                       >  1:7065/1‑140 (MQ=255)                                                 agagGTGGTTTGCTCATCTTTATCCCTCATCGGAAAATGCGGAAATAAGCATACCCTGCCAGTTATGGTGTTGTCATCCGCCCACCCTCGCCACTAAACTGGAAGCAAGACCGTAGGCATTCCGCTTACGAAAAAATAAc                                                                       >  1:412586/1‑140 (MQ=255)                                                     gtggtTTGCTCATCTTTATCCCTCATCGGAAAATGCGGAAATAAGCATACCCTGCCAGTTATGGTGTTGTCATCCGCCCACCCTCGCCACTAAACTGGAAGCAAGACCGTAGGCATTCCGCTTACGAAAAAATAACGAAt                                                                   >  1:126478/1‑140 (MQ=255)                                                        gtTTGCTCATCTTTATCCCTCATCGGAAAATGCGGAAATAAGCATACCCTGCCAGTTATGGTGTTGTCATCCGCCCACCCTCGCCACTAAACTTGAAGCAAGACCGTAGGCATTCCGCTTACGAAAAAATAACGAATTCa                                                                >  1:893320/1‑140 (MQ=255)                                                        gtTTGCTCATCTTTATCCCTCATCGGAAAATGCGGAAATAAGCATACCCTGCCAGTTATGGTGTTGTCATCCGCCCACCCTCGCCACTAAACTGGAAGCAAGACCGTAGGCATTCCGCTTACGAAAAAATAACGAATTCa                                                                <  2:337515/140‑1 (MQ=255)                                                                          cTCATCGGAAAATGCGGAAATAAGCATACCCTGCCAGTTATGGTGTTGTCATCCGCCCACCCTCGCCACTAAACTGGAAGCAAGACCGTAGGCATTCCGCTTACGAAAAAATAACGAATTCAAGGAACTAAGATGCtt                                                >  2:801581/1‑138 (MQ=255)                                                                          cTCATCGGAAAATGCGGAAATAAGCATACCCTGCCAGTTATGGTGTTGTCATCCGCCCACCCTCGCCACTAAACTGGAAGCAAGACCGTAGGCATTCCGCTTACGAAAAAATAACGAATTCAAGGAACTAAGATGCtt                                                <  1:801581/138‑1 (MQ=255)                                                                            cATCGGAAAATGCGGAAATAAGCATACCCTGCCAGTTATGGTGTTGTCATCCGCCCACCCTCGCCACTAAACTGGAAGCAAGACCGTAGGCATTCCGCTTACGAAAAAATAACGAATTCAAGGAACTAAGATGCTTTGGt                                            >  1:375176/1‑140 (MQ=255)                                                                            cATCGGAAAATGCGGAAATAAGCATACCCTGCCAGTTATGGTGTTGTCATCCGCCCACCCTCGCCACTAAACTGGAAGCAAGACCGTAGGCATTCCGCTTACGAAAAAATAACGAATTCAAGGAACTAAGATGCTTTGGt                                            >  2:556227/1‑140 (MQ=255)                                                                            cATCGGAAAATGCGGAAATAAGCATACCCTGCCAGTTATGGTGTTGTCATCCGCCCACCCTCGCCACTAAACTGGAAGCAAGACCGTAGGCATTCCGCTTACGAAAAAATAACGAATTCAAGGAACTAAGATGCTTTGGt                                            >  2:798072/1‑140 (MQ=255)                                                                                    aaTGCGGAAATAAGCATACCCTGCCAGTTATGGTGTTGTCATCCGCCCACCCTCGCCACTAAACTGGAAGCAAGACCGTAGGCATTCCGCTTACGAAAAAATAACGAATTCAAGGAACTAAGATGCTTTgg                                             <  1:901412/131‑1 (MQ=255)                                                                                    aaTGCGGAAATAAGCATACCCTGCCAGTTATGGTGTTGTCATCCGCCCACCCTCGCCACTAAACTGGAAGCAAGACCGTAGGCATTCCGCTTACGAAAAAATAACGAATTCAAGGAACTAAGATGCTTTgg                                             >  2:901412/1‑131 (MQ=255)                                                                                                     aCCCTGCCAGTTATGGTGTTGTCATCCGCCCACCCTCGCCACTAAACTGGAAGCAAGACCGTAGGCATTCCGCTTACGAAAAAATAACGAATTCAAGGAACTAAGATGCTTTGGTCGTTTATCGCTGTCTGTCTTTCCGc                   >  1:2649/1‑140 (MQ=255)                                                                                                        cTGCCAGTTATGGTGTTGTCATCCGCCCACCCTCGCCACTAAACTGGAAGCAAGACCGTAGGCATTCCGCTTACGAAAAAATAACGAATTCAAGGAACTAAGATGCTTTGGTCGTTTATCGCTGTCTGTCTTTCCGCATg                <  1:223637/140‑1 (MQ=255)                                                                                                         tGCCAGTTATGGTGTTGTCATCCGCCCACCCTCGCCACTAAACTGGAAGCAAGACCGTAGGCATTCCGCTTACGAAAAAATAACGAATTCAAGGAACTAAGATGCTTTGGTCGTTTATCGCTGTCTGTCTTTCCGCATgg               <  2:602702/140‑1 (MQ=255)                                                                                                         tGCCAGTTATGGTGTTGTCATCCGCCCACCCTCGCCACTAAACTGGAAGCAAGACCGTAGGCATTCCACTTACGAAAAAATAACGAATTCAAGGAACTAAGATGCTTTGGTCGTTTATCGCTGTCTGTCTTTCCGCATgg               <  2:542951/140‑1 (MQ=255)                                                                                                          gCCAGTTATGGTGTTGTCATCCGCCCACCCTCGCCACTAAACTGGAAGCAAGACCGTAGGCATTCCGCTTACGAAAAAATAACGAATTCAAGGAACTAAGATGCTTTGGTCGTTTATCGCTGTCTGTCTTTCCGCATGGc              >  2:808785/1‑140 (MQ=255)                                                                                                           ccAGTTATGGTGTTGTCATCCGCCCACCCTCGCCACTAAACTTGAAGCAAGACCGTAGGCATTCCGCTTACGAAAAAATAACGAATTCAAGGAACTAAGATGCTTTGGTCGTTTATCGCTGTCTGTCTTTCCGCATGGCt             <  2:893320/140‑1 (MQ=255)                                                                                                           ccAGTTATGGTGTTGTCATCCGCCCACCCTCGCCACTAAACTGGAAGCAAGACCGTAGGCATTCCGCTTACGAAAAAATAACGAATTCAAGGAACTAAGATGCTTTGGTCGTTTATCGCTGTCTGTCTTTCCGCATGGCt             >  1:525469/1‑140 (MQ=255)                                                                                                           ccAGTTATGGTGTTGTCATCCGCCCACCCTCGCCACTAAACTGGAAGCAAGACCGTAGGCATTCCGCTTACGAAAAAATAACGAATTCAAGGAACTAAGATGCTTTGGTCGTTTATCGCTGTCTGTCTTTCCGCATGGCt             >  1:336675/1‑140 (MQ=255)                                                                                                           ccAGTTATGGTGTTGTCATCCGCCCACCCTCGCCACTAAACTGGAAGCAAGACCGTAGGCATTCCGCTTACGAAAAAATAACGAATTCAAGGAACTAAGATGCTTTGGTCGTTTATCGCTGTCTGTCTTTCCGCATGGCt             >  2:768910/1‑140 (MQ=255)                                                                                                                tATGGTGTTGTCATCCGCCCACCCTCGCCACTAAACTGGAAGCAAGACCGTAGGCATTCCGCTTACGAAAAAATAACGAATTCAAGGAACTAAGATGCTTTGGTCGTTTATCGCTGTCTGTCTTTCCGCATGGCTATCtg        >  1:155467/1‑140 (MQ=255)                                                                                                                 aTGGTGTTGTCATCCGCCCACCCTCGCCACTAAACTGGAAGCAAGACCGTAGGCATTCCGCTTACGAAAAAATAACGAATTCAAGGAACTAAGATGCTTTGGTCGTTTATCGCTGTCTGTCTTTCCGCATGGCTATCtgt       >  1:319085/1‑140 (MQ=255)                                                                                                                   ggTGTTGTCATCCGCCCACCCTCGCCACTAAACTGGAAGCAAGACCGTAGGCATTCCGCTTACGAAAAAATAACGAATTCAAGGAACTAAGATGCTTTGGTCGTTTATCGCTGTCTGTCTTTCCGCATGGCTATCTGTgg     <  2:319085/140‑1 (MQ=255)                                                                                                                     tgttgtCATCCGCCCTCCCTCGCCACTAAACTGGAAGCAAGACCGTAGGCATTCCGCTTACGGAAAAATAACGAATTCAAGGAACTAAGATGCTTTGGTCGTTTATCGCTGTCTGTCTTTCCGCATGGCTATCTGTGGAt   <  1:808785/140‑1 (MQ=255)                                                                                                                     tgttgtCATCCGCCCACCCTCGCCACTAAACTGGAAGCAAGACCGTAGGCATTCCGCTTACGAAAAAATAACGAATTCAAGGAACTAAGATGCTTTGGTCGTTTATCGCTGTCTGTCTTTCCGCATGGCTATCTGTGGAt   <  1:556227/140‑1 (MQ=255)                                                                                                                     tgttgtCATCCGCCCACCCTCGCCACTAAACTGGAAGCAAGACCGTAGGCATTCCGCTTACGAAAAAATAACGAATTCAAGGAACTAAGATGCTTTGGTCGTTTATCGCTGTCTGTCTTTCCGCATGGCTATCTGTGGAt   <  1:768910/140‑1 (MQ=255)                                                                                                                     tgttgtCATCCGCCCACCCTCGCCACTAAACTGGAAGCAAGACCGTAGGCATTCCGCTTACGAAAAAATAAAGAATTCAAGGAACTAAGATGCTTTGGTCGTTTATCGCTGTCTGTCTTTCCGCATGGCTATCTGTGGAt   >  2:660723/1‑140 (MQ=255)                                                                                                                      gttgtCATCCGCCCACCCTCGCCACTAAACTGGAAGCAAGACCGTAGGCATTCCGCTTACGAAAAAATAACGAATTCAAGGAACTAAGATGCTTTGGTCGTTTATCGCTGTCTGTCTTTCCGCATGGCTATCTGTGGATg  <  2:155467/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                 CGAAACGATGCGGCGACGACAATTAAGCCAATGATAACAATAAAGAAAAGAGGTGGTTTGCTCATCTTTATCCCTCATCGGAAAATGCGGAAATAAGCATACCCTGCCAGTTATGGTGTTGTCATCCGTCCACCCTCGCCACTAAACTGGAAGCAAGACCGTAGGCATTCCGCTTACGAAAAAATAACGAATTCAAGGAACTAAGATGCTTTGGTCGTTTATCGCTGTCTGTCTTTCCGCATGGCTATCTGTGGATG  >  NC_000913/3605546‑3605802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑