Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,166,562	G→T	A299S (GCA→TCA)	yegQ →	putative peptidase YegQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,166,562	0	G	T	100.0%	143.3 / NA	41	A299S (GCA→TCA)	yegQ	putative peptidase YegQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (20/21);  total (20/21)

GCGTTTGAAGATGAGCACGGCACTTACATCATGAACTCGAAAGATCTGCGCGCCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTGCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGTTCGATACCAGCCTGCTGGAAACTCTGGAAGGTCTGGCGCATCGTGGCTATACCGAAGGTTTCCTGCGTCGTCATACTCA  >  NC_000913/2166427‑2166698                                                                                                                                        |                                                                                                                                         gcgTTTGAAGATGAGCACGGCACTTACATCATGAACTCGAAAGATCTGCGCGCCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTtcacg                                                                                                                                      <  1:391144/140‑1 (MQ=255)  cgTTTGAAGATGAGCACGGCACTTACATCATGAACTCGAAAGATCTGCGCGCCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTtcacgc                                                                                                                                     >  2:79162/1‑140 (MQ=255)  cgTTTGAAGATGAGCACGGCACTTACATCATGAACTCGAAAGATCTGCGCGCCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTtcacgc                                                                                                                                     >  2:406542/1‑140 (MQ=255)  cgTTTGAAGATGAGCACGGCACTTACATCATGAACTCGAAAGATCTGCGCGCCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTtcacgc                                                                                                                                     >  2:580613/1‑140 (MQ=255)  cgTTTGAAGATGAGCACGGCACTTACATCATGAACTCGAAAGATCTGCGCGCCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTtcacgc                                                                                                                                     >  1:609676/1‑140 (MQ=255)  cgTTTGAAGATGAGCACGGCACTTACATCATGAACTCGAAAGATCTGCGCGCCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTtcacgc                                                                                                                                     >  1:454969/1‑140 (MQ=255)  cgTTTGAAGATGAGCACGGCACTTACATCATGAACTCGAAAGATCTGCGCGCCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTtca                                                                                                                                        >  2:629314/1‑137 (MQ=255)              aGCACGGCACTTACATCATGAACTCGAAAGATCTGCGCGCCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTTCACGCACCGCACAGGtt                                                                                                                         >  1:271079/1‑140 (MQ=255)               gCACGGCACTTACATCATGAACTCGAAAGATCTGCGCGCCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTTCACGCACCGCACAGGttt                                                                                                                        >  2:270839/1‑140 (MQ=255)                  cGGCACTTACATCATGAACTCGAAAGATCTGCGCGCCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTTCACGCACCGCACAGGTTTAcc                                                                                                                     >  1:73095/1‑140 (MQ=255)                             tcatGAACTCGAAAGATCTGCGCGCCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTTCACGCACCGCACAGGTTTACCGCAAAGCTATc                                                                                                          >  1:679993/1‑140 (MQ=255)                               atGAACTCGAAAGATCTGCGCGCCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTTCACGCACCGCACAGGTTTACCGCAAAGCTATCGa                                                                                                        >  1:46935/1‑140 (MQ=255)                               atGAACTCGAAAGATCTGCGCGCCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTTCACGCACCGCACAGGTTTACCGCAAAGCTATCGa                                                                                                        >  2:599943/1‑140 (MQ=255)                                  aaCTCGAAAGATCTGCGCGCCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTTCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGa                                                                                                     >  1:33435/1‑140 (MQ=255)                                      cgtaaGATCTGCGCGCCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTTCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGAcgcc                                                                                                 >  1:427741/4‑140 (MQ=255)                                          aGATCTGCGCGCCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTTCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTg                                                                                             <  1:111743/140‑1 (MQ=255)                                                   cgcCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTTCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAAc                                                                                    >  2:6310/1‑140 (MQ=255)                                                   cgcCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTTCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAAc                                                                                    <  2:512463/140‑1 (MQ=255)                                                      cATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTTCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGt                                                                                 >  2:375048/1‑140 (MQ=255)                                                       aTCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGATGGTCGTACCAAATCTTTCTACTATTGTTCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGtt                                                                                <  2:180805/140‑1 (MQ=255)                                                       aTCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTTCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGtt                                                                                <  2:46935/140‑1 (MQ=255)                                                       aTCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTTCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGtt                                                                                <  2:454969/140‑1 (MQ=255)                                                       aTCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTTCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGtt                                                                                <  2:73095/140‑1 (MQ=255)                                                                    aaCGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTTCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGTTCGATACCAGCctg                                                                   >  2:64496/1‑140 (MQ=255)                                                                      cGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTTCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGTTCGATACCAGCctgct                                                                 >  1:408584/1‑140 (MQ=255)                                                                        ccTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTTCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGTTCGATACCAGCCTGCTgg                                                               <  2:589222/140‑1 (MQ=255)                                                                        ccTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTTCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGTTCGATACCAGCCTGCTgg                                                               >  2:146091/1‑140 (MQ=255)                                                                        ccTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTTCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGTTCGATACCAGCCTGCTgg                                                               >  1:45724/1‑140 (MQ=255)                                                                           gACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTTCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCTCTGCGGGAAAACCGTTCGATACCAGCCTGCTGGaaa                                                            <  2:636103/140‑1 (MQ=255)                                                                           gACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTTCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGTTCGATACCAGCCTGCTGGaaa                                                            <  2:25414/140‑1 (MQ=255)                                                                             ccAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTTCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGTTCGATACCAGCCTGCTGGAAAct                                                          <  1:79162/140‑1 (MQ=255)                                                                                  aTGGGCGTGCATTCGCTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTTCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGTTCGATACCAGCCTGCTGGAAACTCTGGa                                                     <  1:642071/140‑1 (MQ=255)                                                                                          gCATTCGCTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTTCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGTTCGATACCAGCCTGCTGGAAACTCTGGAAGGTCTgg                                             <  2:358446/140‑1 (MQ=255)                                                                                           cATTCGCTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTTCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGTTCGATACCAGCCTGCTGGAAACTCTGGAAGGTCTGgc                                            >  2:116972/1‑140 (MQ=255)                                                                                               cGCTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTTCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGTTCGATACCAGCCTGCTGGAAACTCTGGAAGGTCTGGCGCAt                                        <  1:697027/140‑1 (MQ=255)                                                                                                 cTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTTCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGTTCGATACCAGCCTGCTGGAAACTCTGGATGGTCTGGCGCATCg                                      <  1:574596/140‑1 (MQ=255)                                                                                                     aaaTCGAAGGTCGTACCAAATCTTTCTACTATTGTTCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGTTCGATACCAGCCTGCTGGAAACTCTGGAAGGTCTGGCGCATCGTGGc                                  <  1:116972/140‑1 (MQ=255)                                                                                                     aaaTCGAAGGTCGTACCAAATCTTTCTACTATTGTTCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGTTCGATACCAGCCTGCTGGAAACTCTGGAAGGTCTGGCGCATCGTGGc                                  <  2:339999/140‑1 (MQ=255)                                                                                                      aaTCGAAGGTCGTACCAAATCTTTCTACTATTGTTCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGTTCGATACCAGCCTGCTGGAAACTCTGGAAGGTCTGGCGCATCGTGGCt                                 <  1:64496/140‑1 (MQ=255)                                                                                                          gAAGGTCGTACCAAATCTTTCTACTATTGTTCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGTTCGATACCAGCCTGCTGGAAACTCTGGAAGGTCTGGCGCATCGTGGCTATAc                             <  2:427741/140‑1 (MQ=255)                                                                                                              gTCGTACCAAATCTTTCTACTATTGTTCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGTTCGATACCAGCCTGCTGGAAACTCTGGAAGGTCTGGCGCATCGTGGCTATACCGaa                         >  2:41257/1‑140 (MQ=255)                                                                                                                    ccAAATCTTTCTACTATTGTTCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGTTCGATACCAGCCTGCTGGAAACTCTGGAAGGTCTGGCGCATCGTGGCTATACCGAAGGTTTc                   <  1:6310/140‑1 (MQ=255)                                                                                                                       aaTCTTTCTACTATTGTTCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGTTCGATACCAGCCTGCTGGAAACTCTGGAAGGTCTGGCGCATCGTGGCTATACCGAAGGTTTCCTg                <  2:679993/140‑1 (MQ=255)                                                                                                                             tCTACTATTGTTCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGTTCGATACCAGCCTGCTGGAAACTCTGGAAGGTCTGGCGCATCGTGGCTATACCGAAGGTTTCCTGcgtcgt          >  1:422475/1‑140 (MQ=255)                                                                                                                             tCTACTATTGTTCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGTTCGATACCAGCCTGCTGGAAACTCTGGAAGGTCTGGCGCATCGTGGCTATACCGAAGGTTTCCTGcgtcgt          >  1:125247/1‑140 (MQ=255)                                                                                                                             tCTACTATTGTTCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGTTCGATACCAGCCTGCTGGAAACTCTGGAAGGTCTGGCGCATCGTGGCTATACCGAAGGTTTCCTGcg              <  2:136058/136‑1 (MQ=255)                                                                                                                             tCTACTATTGTTCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGTTCGATACCAGCCTGCTGGAAACTCTGGAAGGTCTGGCGCATCGTGGCTATACCGAAGGTTTCCTGcg              >  1:136058/1‑136 (MQ=255)                                                                                                                                   aTTGTTCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGTTCGATACCAGCCTGCTGGAAACTCTGGAAGGTCTGGCGCATCGTGGCTATACCGAAGGTTTCCTGCGTCGTCATACt    >  2:226286/1‑140 (MQ=255)                                                                                                                                     tgttCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGTTCGATACCAGCCTGCTGGAAACTCTGGAAGGTCTGGCGCATCGTGGCTATACCGAAGGTTTCCTGCGTCGTCATACTCa  <  2:408584/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         GCGTTTGAAGATGAGCACGGCACTTACATCATGAACTCGAAAGATCTGCGCGCCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTGCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGTTCGATACCAGCCTGCTGGAAACTCTGGAAGGTCTGGCGCATCGTGGCTATACCGAAGGTTTCCTGCGTCGTCATACTCA  >  NC_000913/2166427‑2166698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑