Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,068,851	G→T	intergenic (‑43/+96)	argO ← / ← mscS	L‑arginine exporter/small conductance mechanosensitive channel MscS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,068,851	0	G	T	100.0%	124.7 / NA	36	intergenic (‑43/+96)	argO/mscS	L‑arginine exporter/small conductance mechanosensitive channel MscS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (17/19);  total (17/19)

GTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCAC  >  NC_000913/3068715‑3068985                                                                                                                                         |                                                                                                                                       gTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCTCgg                                                                                                                                     >  2:28023/1‑140 (MQ=255)   aTGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCTCGGGa                                                                                                                                   >  1:627376/1‑140 (MQ=255)           aTTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCTCGGGATTGttatt                                                                                                                           >  2:307110/1‑140 (MQ=255)                       aGCATTTTGTGGACCTAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCTCGGGATTGTTATTAGCGGAAATTGa                                                                                                               <  1:435622/140‑1 (MQ=255)                        gCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCTCGGGATTGTTATTAGCGGAAATTGat                                                                                                              <  1:638476/140‑1 (MQ=255)                              tgtgGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCTCGGGATTGTTATTAGCGGAAATTGATAATAtt                                                                                                        <  1:606669/140‑1 (MQ=255)                                        gCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCTCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAAt                                                                                              <  1:254895/140‑1 (MQ=255)                                        gCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCTCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAAt                                                                                              <  1:567786/140‑1 (MQ=255)                                                 tCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCTCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAAc                                                                                     <  1:538963/140‑1 (MQ=255)                                                    tAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCTCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACtaa                                                                                  <  1:589044/140‑1 (MQ=255)                                                      gccgccCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCTCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACtaata                                                                                >  1:207922/1‑140 (MQ=255)                                                         gccCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCTCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAaga                                                                             >  2:664768/1‑140 (MQ=255)                                                          ccccAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCTCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAagag                                                                            >  2:282898/1‑140 (MQ=255)                                                          ccccAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCTCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAagag                                                                            >  1:624927/1‑140 (MQ=255)                                                            ccAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCTCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAagagag                                                                          <  2:189827/140‑1 (MQ=255)                                                            ccAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCTCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAagagag                                                                          <  1:119707/140‑1 (MQ=255)                                                             cAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCTCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAagagaga                                                                         <  2:196988/140‑1 (MQ=255)                                                               aGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCTCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAgg                                                                       >  1:335634/1‑140 (MQ=255)                                                                   cAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCTCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCag                                                                   <  1:64272/140‑1 (MQ=255)                                                                    aaGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCTCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCaga                                                                  <  1:28023/140‑1 (MQ=255)                                                                           tGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCTCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGtatt                                                           >  1:420010/1‑140 (MQ=255)                                                                                   ataaGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCTCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGcc                                                   >  2:311109/1‑140 (MQ=255)                                                                                        aaaaCACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCTCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGc                                              <  1:632429/140‑1 (MQ=255)                                                                                          aaCACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCTCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGt                                            <  2:624927/140‑1 (MQ=255)                                                                                           acacGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCTCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGtt                                           >  1:347492/1‑140 (MQ=255)                                                                                           acacGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCTCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGtt                                           >  2:202386/1‑140 (MQ=255)                                                                                                 tGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCTCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTAc                                     <  2:502698/140‑1 (MQ=255)                                                                                                  ggATATTCCGAATTAAGTATCTGATGCAGAGACTATATCTCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACg                                    <  2:335634/140‑1 (MQ=255)                                                                                                       ttCCGAATTAAGTATCTGATGCAGAGACTATATCTCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCt                               <  1:664768/140‑1 (MQ=255)                                                                                                           gAATTAAGTATCTGATGCAGAGACTATATCTCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGt                           <  2:307467/140‑1 (MQ=255)                                                                                                           gAATTAAGTATCTGATGCAGAGACTATATCTCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGt                           <  2:627376/140‑1 (MQ=255)                                                                                                                      cTGATGCAGAGACTATATCTCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCAcc                >  2:526584/1‑140 (MQ=255)                                                                                                                          tGCAGAGACTATATCTCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGATTTGTCTTCTTTCACCCGCt            >  2:88942/1‑140 (MQ=255)                                                                                                                                 aCTATATCTCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGtt     >  1:404020/1‑140 (MQ=255)                                                                                                                                 aCTATATCTCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGtt     >  2:373512/1‑140 (MQ=255)                                                                                                                                    atatCTCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTcac  >  1:285850/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       GTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCAC  >  NC_000913/3068715‑3068985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑