Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,118,565	G→T	A183E (GCG→GAG)	yghJ ←	putative lipoprotein YghJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,118,565	0	G	T	100.0%	128.0 / NA	38	A183E (GCG→GAG)	yghJ	putative lipoprotein YghJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (18/20);  total (18/20)

GCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACT  >  NC_000913/3118430‑3118700                                                                                                                                        |                                                                                                                                        gcagcaTTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCTCCgg                                                                                                                                     <  1:139553/140‑1 (MQ=255)   agcaTTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCTCCGGAc                                                                                                                                   <  1:623807/140‑1 (MQ=255)     caTTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCTCCGGACAg                                                                                                                                 <  1:138094/140‑1 (MQ=255)            ttCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCTCCGGACAGCTGTTAc                                                                                                                          <  1:377247/140‑1 (MQ=255)              ccACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCTCCGGACAGCTGTTACTg                                                                                                                        >  1:173268/1‑140 (MQ=255)               cACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCTCCGGACAg                                                                                                                                 <  2:377543/130‑1 (MQ=255)               cACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCTCCGGACAg                                                                                                                                 >  1:377543/1‑130 (MQ=255)               cACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCTCCGGACAGCTGTTACTgg                                                                                                                       >  1:667725/1‑140 (MQ=255)                    ctTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCTCCGGACAGCTGTTACTGGACGtt                                                                                                                  >  2:608533/1‑140 (MQ=255)                    ctTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCTCCGGACAGCTGTTACTGGACGtt                                                                                                                  >  2:509510/1‑140 (MQ=255)                    ctTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCTCCGGACAGCTGTTACTGGACGtt                                                                                                                  >  1:595255/1‑140 (MQ=255)                            aCCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCTCCGGACAGCTGTTACTGGACGTTACCAGCGa                                                                                                          >  2:625656/1‑140 (MQ=255)                            aCCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCTCCGGACAGCTGTTACTGGACGTTACCAGCGa                                                                                                          >  1:378412/1‑140 (MQ=255)                            aCCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCTCCGGACAGCTGTTACTGGACGTTACCAGCGa                                                                                                          <  1:603785/140‑1 (MQ=255)                                gCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCTCCGGACAGCTGTTACTGGACGTTACCAGGGAAAcc                                                                                                      >  2:433687/1‑140 (MQ=255)                                 cTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCTCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCg                                                                                                     <  2:154038/140‑1 (MQ=255)                                      tGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCTCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTg                                                                                                <  2:595702/140‑1 (MQ=255)                                         aCTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCTCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCtt                                                                                             <  2:173268/140‑1 (MQ=255)                                               ccGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCTCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTg                                                                                       >  1:222853/1‑140 (MQ=255)                                                             aTTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCTCCGGACAGCTGTTACTGGACGTTACCAGGGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGc                                                                         <  1:433687/140‑1 (MQ=255)                                                                  cTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCTCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGtt                                                                    >  1:377321/1‑140 (MQ=255)                                                                           cGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCTCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGt                                                           >  2:138363/1‑140 (MQ=255)                                                                            gAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCTCCGGACAGCTG‑TACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTcc                                                         >  1:118619/1‑140 (MQ=255)                                                                              gTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCTCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCt                                                        <  1:250030/140‑1 (MQ=255)                                                                                   aGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCTCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAgg                                                   <  2:377321/140‑1 (MQ=255)                                                                                            tctcGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCTCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGac                                          <  1:450665/140‑1 (MQ=255)                                                                                                 aTCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCTCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACttt                                     >  2:403151/1‑140 (MQ=255)                                                                                                     ccGAGGAGAACGTCAGACAAACCTGTTCTGTATTCTCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCa                                 <  1:509510/140‑1 (MQ=255)                                                                                                       gaggagAACGTCAGACAAACCTGTTCTGTATTCTCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAAc                               <  1:625656/140‑1 (MQ=255)                                                                                                             aaCGTCAGACAAACCTGTTCTGTATTCTCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACcgcacg                         >  2:455070/1‑140 (MQ=255)                                                                                                               cGTCAGACAAACCTGTTCTGTATTCTCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACcgcacgca                       >  1:285786/1‑140 (MQ=255)                                                                                                                        aaCCTGTTCTGTATTCTCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTAcgcg              <  1:403151/140‑1 (MQ=255)                                                                                                                              ttCTGTATTCTCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCtt        >  2:427667/1‑140 (MQ=255)                                                                                                                              ttCTGTATTCTCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCtt        >  1:410957/1‑140 (MQ=255)                                                                                                                               tCTGTATTCTCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTc       <  2:695357/140‑1 (MQ=255)                                                                                                                                  gTATTCTCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTctga    <  2:667725/140‑1 (MQ=255)                                                                                                                                    aTTCTCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTctgact  <  2:595255/140‑1 (MQ=255)                                                                                                                                    aTTCTCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTctgact  <  1:3872/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        GCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACT  >  NC_000913/3118430‑3118700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑