Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,269,368	C→A	T318T (ACC→ACA)	yhaC →	uncharacterized protein YhaC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,269,368	0	C	A	100.0%	190.5 / NA	54	T318T (ACC→ACA)	yhaC	uncharacterized protein YhaC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (32/22);  total (32/22)

CAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGG  >  NC_000913/3269235‑3269504                                                                                                                                      |                                                                                                                                         cAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGaa                                                                                                                                    <  1:680222/140‑1 (MQ=255)     aGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATg                                                                                                                                >  1:285115/1‑140 (MQ=255)          tatataAAAGCTCATCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTAt                                                                                                                           >  2:430222/1‑140 (MQ=255)                aaaGCTCATCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGgtc                                                                                                                     >  2:267516/1‑140 (MQ=255)                    cTCATCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTcc                                                                                                                 >  2:205368/1‑140 (MQ=255)                     tCATCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTccc                                                                                                                >  1:697840/1‑140 (MQ=255)                      cATCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATc                                                                                                                          >  2:61041/1‑129 (MQ=255)                      cATCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATc                                                                                                                          <  1:61041/129‑1 (MQ=255)                      cATCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTcccc                                                                                                               >  2:502939/1‑140 (MQ=255)                      cATCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTcccc                                                                                                               <  2:636776/140‑1 (MQ=255)                       aTCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTccccc                                                                                                              >  1:159724/1‑140 (MQ=255)                       aTCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTccccc                                                                                                              >  1:388055/1‑140 (MQ=255)                       aTCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTccccc                                                                                                              >  1:491600/1‑140 (MQ=255)                        tCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGgtcgt                                                                                                                   >  1:697560/1‑134 (MQ=255)                        tCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGgtcgt                                                                                                                   <  2:697560/134‑1 (MQ=255)                        tCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCCTATCTGGTCGTCCCCCa                                                                                                             <  2:285115/140‑1 (MQ=255)                         cGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTCCCCCaa                                                                                                            <  2:254644/140‑1 (MQ=255)                         cGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTCCCCCaa                                                                                                            <  1:71545/140‑1 (MQ=255)                         cGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTCCCCCaa                                                                                                            <  1:724803/140‑1 (MQ=255)                         cGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTCCCCCaa                                                                                                            <  2:48867/140‑1 (MQ=255)                                 atcaATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTCCCCCAAattaatta                                                                                                    <  2:491600/140‑1 (MQ=255)                                        gCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATg                                                                                             <  1:299393/140‑1 (MQ=255)                                         cTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATgg                                                                                            >  1:296947/1‑140 (MQ=255)                                           tGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGAt                                                                                          >  1:626452/1‑140 (MQ=255)                                             aaCAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGAttt                                                                                        <  1:205368/140‑1 (MQ=255)                                               cAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTaa                                                                                      <  1:430222/140‑1 (MQ=255)                                                  aattaaattaACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACa                                                                                   >  2:645647/1‑140 (MQ=255)                                                  aattaaattaACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACa                                                                                   >  1:316734/1‑140 (MQ=255)                                                       aattaaCAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATagaa                                                                              <  2:632717/140‑1 (MQ=255)                                                       aattaaCAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATagaa                                                                              <  1:531617/140‑1 (MQ=255)                                                           aaCAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGAct                                                                          <  2:296947/140‑1 (MQ=255)                                                            aCAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGActc                                                                         >  1:8624/1‑140 (MQ=255)                                                            aCAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGActc                                                                         >  1:168607/1‑140 (MQ=255)                                                                 gaTGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCt                                                                    <  1:153352/140‑1 (MQ=255)                                                                  aTGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTc                                                                   >  2:654257/1‑140 (MQ=255)                                                                      aaGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCagaa                                                               <  2:168607/140‑1 (MQ=255)                                                                          aTTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGAct                                                                          >  2:689063/1‑125 (MQ=255)                                                                          aTTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGAct                                                                          <  1:689063/125‑1 (MQ=255)                                                                          aTTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGt                                                           <  2:623316/140‑1 (MQ=255)                                                                            tctcATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTa                                                         >  2:369321/1‑140 (MQ=255)                                                                                               tGATTACATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCg                                      >  2:655682/1‑140 (MQ=255)                                                                                                     cATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAg                                >  2:73697/1‑140 (MQ=255)                                                                                                     cATCGATATAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAg                                >  2:400540/1‑140 (MQ=255)                                                                                                          atatAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGaa                           >  1:245565/1‑140 (MQ=255)                                                                                                          atatAATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGaa                           >  1:11086/1‑140 (MQ=255)                                                                                                            ataATCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGaaaa                         >  2:259098/1‑140 (MQ=255)                                                                                                                tCATTGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATtct                     >  2:573279/1‑140 (MQ=255)                                                                                                                    tGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCgg                 >  2:491457/1‑140 (MQ=255)                                                                                                                    tGAAGATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCgg                 >  1:82636/1‑140 (MQ=255)                                                                                                                        gATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCGGACaa             >  2:138869/1‑140 (MQ=255)                                                                                                                        gATTATGAAAATACAAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCGGACaa             <  1:259098/140‑1 (MQ=255)                                                                                                                           tATGAAAATACAAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCGGACAATgg          >  1:529765/1‑140 (MQ=255)                                                                                                                           tATGAAAATACAAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCGGACAATgg          >  1:173570/1‑140 (MQ=255)                                                                                                                                   tACAAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAgg  <  2:626452/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         CAAAAGAAATATATAAAAGCTCATCGAAAATCATCAATTGCTTGAACAGAATTAAATTAACAGAGATGAAAGAATTCTCATCAGAAAAAATATATGATTACATCGATATAATCATTGAAGATTATGAAAATACCAAAGAAAATGCTTATCTGGTCGTCCCCCAAATTAATTATACTATGGATTTAAACATAGAAGACTCTAGCTCAGAAGAGTTACTTTCAGATAATACCCTCGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGG  >  NC_000913/3269235‑3269504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑