Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,462,869	G→T	E176* (GAG→TAG)	gspJ →	Type II secretion system protein GspJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,462,869	0	G	T	100.0%	227.6 / NA	63	E176* (GAG→TAG)	gspJ	Type II secretion system protein GspJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (35/28);  total (35/28)

CCCAGGCGATGCTGGAACATGTCGAGAGCTTTCTTCTGGAAAGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAG  >  NC_000913/3462732‑3462996                                                                                                                                          |                                                                                                                                cccAGGCGATGCTGGAACATGTCGAGAGCTTTCTTCTGGAAAGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAg                                                                                                                               >  1:410072/1‑140 (MQ=255) cccAGGCGATGCTGGAACATGTCGAGAGCTTTCTTCTGGAAAGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAg                                                                                                                               >  2:510608/1‑140 (MQ=255) cccAGGCGATGCTGGAACATGTCGAGAGCTTTCTTCTGGAAAGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAg                                                                                                                               >  1:259904/1‑140 (MQ=255)  ccAGGCGATGCTGGAACATGTCGAGAGCTTTCTTCTGGAAAGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGc                                                                                                                              >  2:364592/1‑140 (MQ=255)        gATGCTGGAACATGTCGAGAGCTTTCTTCTGGAAAGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAg                                                                                                                        <  1:136202/140‑1 (MQ=255)          tGCTGGAACATGTCGAGAGCTTTCTTCTGGAAAGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCg                                                                                                                      >  2:1659/1‑140 (MQ=255)           gCTGGAACATGTCGAGAGCTTTCTTCTGGAAAGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCga                                                                                                                     >  1:630842/1‑140 (MQ=255)                  cATGTCGAGAGCTTTCTTCTGGAAAGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGagaaga                                                                                                              <  2:160173/140‑1 (MQ=255)                     gTCGAGAGCTTTCTTCTGGAAAGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTc                                                                                                           >  1:213774/1‑140 (MQ=255)                            gCTTTCTTCTGGAAAGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCt                                                                                                    <  1:490890/140‑1 (MQ=255)                            gCTTTCTTCTGGAAAGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCt                                                                                                    <  2:677091/140‑1 (MQ=255)                                  ttctGGAAAGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACg                                                                                              <  2:9762/140‑1 (MQ=255)                                     tGGAAAGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAg                                                                                           <  2:294441/140‑1 (MQ=255)                                       gAAAGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGc                                                                                         <  1:364592/140‑1 (MQ=255)                                        aaaGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGcc                                                                                        <  2:410072/140‑1 (MQ=255)                                        aaaGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGcc                                                                                        <  2:593612/140‑1 (MQ=255)                                         aaGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCg                                                                                       <  2:259904/140‑1 (MQ=255)                                         aaGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCg                                                                                       <  1:642812/140‑1 (MQ=255)                                            tAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGa                                                                                    >  2:146551/1‑140 (MQ=255)                                            tAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGa                                                                                    >  2:673516/1‑140 (MQ=255)                                                aGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCTGGaataa                                                                                <  1:143088/140‑1 (MQ=255)                                                aGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGaataa                                                                                <  1:268062/140‑1 (MQ=255)                                                    gggAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAAc                                                                            >  2:16614/1‑140 (MQ=255)                                                       aaaGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCAt                                                                         <  1:12899/140‑1 (MQ=255)                                                               gAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATg                                                                 >  1:175095/1‑140 (MQ=255)                                                               gAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATg                                                                 >  2:23826/1‑140 (MQ=255)                                                                    tCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGaacaa                                                            >  2:85332/1‑140 (MQ=255)                                                                     ccTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGaacaac                                                           >  1:115940/1‑140 (MQ=255)                                                                             aGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAAcg                                                          <  1:583625/133‑1 (MQ=255)                                                                             aGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAAcg                                                          >  2:583625/1‑133 (MQ=255)                                                                             aGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTc                                                   >  1:566761/1‑140 (MQ=255)                                                                              gTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTcg                                                  <  2:29511/140‑1 (MQ=255)                                                                                  cGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCgct                                              <  1:420948/140‑1 (MQ=255)                                                                                  cGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCgct                                              <  2:561546/140‑1 (MQ=255)                                                                                    ttACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCgctgc                                            <  1:510608/140‑1 (MQ=255)                                                                                    ttACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCgctgc                                            <  1:258100/140‑1 (MQ=255)                                                                                     tACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCgctgct                                           >  1:310189/1‑140 (MQ=255)                                                                                       cATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCa                                         <  1:48462/140‑1 (MQ=255)                                                                                       cATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCa                                         >  1:182814/1‑140 (MQ=255)                                                                                       cATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCa                                         >  1:406531/1‑140 (MQ=255)                                                                                       cATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCa                                         <  1:411664/140‑1 (MQ=255)                                                                                            gCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTa                                    <  2:246197/140‑1 (MQ=255)                                                                                             cAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTAc                                   >  1:669391/1‑140 (MQ=255)                                                                                                 cTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCAt                               >  2:87269/1‑140 (MQ=255)                                                                                                   cAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGt                             >  1:546232/1‑140 (MQ=255)                                                                                                    aGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGtt                            >  1:390501/1‑140 (MQ=255)                                                                                                    aGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGtt                            >  2:665280/1‑140 (MQ=255)                                                                                                     gCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTa                           <  1:665280/140‑1 (MQ=255)                                                                                                     gCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTa                           >  2:374026/1‑140 (MQ=255)                                                                                                     gCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTa                           >  1:190345/1‑140 (MQ=255)                                                                                                          atGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAGCAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGgc                      >  2:497797/1‑140 (MQ=255)                                                                                                          atGGGGGGTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGgc                      >  1:396763/1‑140 (MQ=255)                                                                                                            ggggggTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGgcgc                    >  1:346400/1‑140 (MQ=255)                                                                                                            ggggggTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGgcgc                    >  1:136966/1‑140 (MQ=255)                                                                                                              ggggTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTc                  >  1:514474/1‑140 (MQ=255)                                                                                                                ggTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCAt                >  2:691827/1‑140 (MQ=255)                                                                                                                 gTTACAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATg               >  1:304052/1‑140 (MQ=255)                                                                                                                    aCAGCGCCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGgct            >  2:106000/1‑140 (MQ=255)                                                                                                                       gcgcCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGgctgct         >  1:608574/1‑140 (MQ=255)                                                                                                                         gcCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGctct       <  2:566761/140‑1 (MQ=255)                                                                                                                         gcCGTTTTGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGctct       <  1:277950/140‑1 (MQ=255)                                                                                                                              tttGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGgcag  <  1:1659/140‑1 (MQ=255)                                                                                                                              tttGCATTACCTTAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGgcag  <  1:468582/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                CCCAGGCGATGCTGGAACATGTCGAGAGCTTTCTTCTGGAAAGTAACAGCGGGGAAAGCCAGGAACTTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAG  >  NC_000913/3462732‑3462996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑