Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,550,043 C→A A10S (GCG→TCG)  malQ ← 4‑alpha‑glucanotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,550,0430CA96.8% 103.5 / ‑2.6 31A10S (GCG→TCG) malQ4‑alpha‑glucanotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (13/17);  total (13/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

GGACTGGCGTTACCGCCACTTTCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGC  >  NC_000913/3549908‑3550174
                                                                                                                                       |                                                                                                                                   
ggccTGGCTTTACCGCCACTTTCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGAGGCa                                                                                                                                 <  2:265866/137‑1 (MQ=255)
ggACTGGCGTTACCGCCACTTTCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGAGGCa                                                                                                                                 <  1:158992/140‑1 (MQ=255)
       gtttACCGCCACTTTCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGAGGCATTATCCa                                                                                                                          <  1:359868/138‑1 (MQ=255)
           aCCGCCACTTTCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGAGGCATTATCCAGACg                                                                                                                      >  1:601751/1‑140 (MQ=255)
                cACTTTCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGAGGCATTATCCAGACGTTTGc                                                                                                                 >  2:90354/1‑140 (MQ=255)
                  cTTTCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGAGGCATTATCCAGACGTTTGCtt                                                                                                               >  1:205768/1‑140 (MQ=255)
                     tCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGAGGCATTATCCAGACGTTTGCTTTcc                                                                                                            <  2:482517/140‑1 (MQ=255)
                     tCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGAGGCATTATCCAGACGTTTGCTTTcc                                                                                                            <  1:515734/140‑1 (MQ=255)
                       gTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGAGGCATTATCCAGACGTTTGCTTTCCAt                                                                                                          <  2:84873/140‑1 (MQ=255)
                                     aTGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGAGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTAGc                                                                                            >  1:581213/1‑140 (MQ=255)
                                           cgcgTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGAGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTAGCGTTTtg                                                                                      <  1:52290/140‑1 (MQ=255)
                                           cgcgTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGAGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTAGCGTTTtg                                                                                      <  2:601751/140‑1 (MQ=255)
                                            gcgTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGAGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTAGCGTTTtgc                                                                                     <  2:304423/140‑1 (MQ=255)
                                             cgTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGAGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTAGCGTTTtgcc                                                                                    >  2:492207/1‑140 (MQ=255)
                                             cgTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGAGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTAGCGTTTtgcc                                                                                    >  2:408920/1‑140 (MQ=255)
                                                 aaGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGAGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTAGCGTTTtgcctgcc                                                                                <  1:447398/140‑1 (MQ=255)
                                                      aaCGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGAGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTAGCGTTTTGCCTGCCAGATa                                                                           <  1:241356/140‑1 (MQ=255)
                                                                            aaTCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGAGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGa                                                                  <  1:716818/127‑1 (MQ=255)
                                                                            aaTCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGAGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGa                                                                  >  2:716818/1‑127 (MQ=255)
                                                                             aTCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATaga                                                    <  1:621861/140‑1 (MQ=255)
                                                                                            ccGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGAGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTaaa                                     >  2:225791/1‑140 (MQ=255)
                                                                                                 ggCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGAGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATAc                                >  2:232693/1‑140 (MQ=255)
                                                                                                          gTAATTGGGGCTAATCCCCGCCGCCAGCGAGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAgcggg                       <  1:195065/140‑1 (MQ=255)
                                                                                                            aaTTGGGGCTAATCCCCGCCGCCAGCGAGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAgcgggcg                     <  1:90354/140‑1 (MQ=255)
                                                                                                            aaTTGGGGCTAATCCCCGCCGCCAGCGAGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAgcgggcg                     <  1:225791/140‑1 (MQ=255)
                                                                                                                  ggCTAATCCCCGCCGCCAGCGAGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAgcggg                       <  2:256867/132‑1 (MQ=255)
                                                                                                                  ggCTAATCCCCGCCGCCAGCGAGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAgcggg                       >  1:256867/1‑132 (MQ=255)
                                                                                                                  ggCTAATCCCCGCCGCCAGCGAGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTg               >  2:295059/1‑140 (MQ=255)
                                                                                                                  ggCTAATCCCCGCCGCCAGCGAGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTg               >  2:616677/1‑140 (MQ=255)
                                                                                                                            cgccgcCAGCGAGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATcgccg     >  2:554822/1‑140 (MQ=255)
                                                                                                                               cgccAGCGAGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCcgcgc  <  1:232693/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                   
GGACTGGCGTTACCGCCACTTTCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGC  >  NC_000913/3549908‑3550174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑