Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,086,840	C→A	C336F (TGC→TTC)	pgaC ←	poly‑N‑acetyl‑D‑glucosamine synthase subunit PgaC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,086,840	0	C	A	100.0%	230.0 / NA	64	C336F (TGC→TTC)	pgaC	poly‑N‑acetyl‑D‑glucosamine synthase subunit PgaC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (38/26);  total (38/26)

GGCTGACAATAAATTGCAGTAAACATAACGTACACAATAATATTCCGGCAGTATGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGCAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCTGAGCCCAGCGCAGGCGC  >  NC_000913/1086706‑1086971                                                                                                                                       |                                                                                                                                    ggCTGACAATAAATTGCAGTAAACATAACGTACACAATAATATTCCGGCAGTATGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTg                                                                                                                                >  2:230873/1‑140 (MQ=255)   cTGACAATAAATTGCAGTAAACATAACGTACACAATAATATTCCGGCAGTATGTGTCGCAGCAATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGaa                                                                                                                              <  1:563262/140‑1 (MQ=255)     gACAATAAATTGCAGTAAACATAACGTACACAATAATATTCCGGCAGTATGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGaa                                                                                                                              <  1:133559/138‑1 (MQ=255)     gACAATAAATTGCAGTAAACATAACGTACACAATAATATTCCGGCAGTATGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGaa                                                                                                                              >  2:133559/1‑138 (MQ=255)     gACAATAAATTGCAGTAAACATAACGTACACAATAATATTCCGGCAGTATGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAgg                                                                                                                            >  2:429123/1‑140 (MQ=255)      aCAATAAATTGCAGTAAACATAACGTACACAATAATATTCCGGCAGTATGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGc                                                                                                                           >  2:467762/1‑140 (MQ=255)            aaTTGCAGTAAACATAACGTACACAATAATATTCCGGCAGTATGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCatat                                                                                                                     <  1:189507/140‑1 (MQ=255)                gCAGT‑AACATAACGTACACAATAATATTCCGGCAGTATGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTgtcg                                                                                                                >  2:157898/1‑140 (MQ=255)                  aGTAAACATAACGTACACAATAATATTCCGGCAGTATGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTgtcgt                                                                                                               <  2:685771/140‑1 (MQ=255)                  aGTAAACATAACGTACACAATAATATTCCGGCAGTATGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTgtcgt                                                                                                               <  1:429123/140‑1 (MQ=255)                    tAAACATAACGTACACAATAATATTCCGGCAGTATGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCa                                                                                                             <  1:467762/140‑1 (MQ=255)                        cATAACGTACACAATAATATTCCGGCAGTATGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCa                                                                                                         <  2:409654/140‑1 (MQ=255)                          tAACGTACACAATAATATTCCGGCAGTATGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGt                                                                                                       <  1:631947/140‑1 (MQ=255)                          tAACGTACACAATAATATTCCGGCAGTATGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGt                                                                                                       <  2:289808/140‑1 (MQ=255)                           aaCGTACACAATAATATTCCGGCAGTATGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTgtcgtc                                                                                                              <  2:597142/132‑1 (MQ=255)                           aaCGTACACAATAATATTCCGGCAGTATGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTgtcgtc                                                                                                              >  1:597142/1‑132 (MQ=255)                           aaCGTACACAATAATATTCCGGCAGTATGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTa                                                                                                      >  1:714723/1‑140 (MQ=255)                             cGTACACAATAATATTCCGGCAGTATGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTAtt                                                                                                    >  2:716297/1‑140 (MQ=255)                              gTACACAATAATATTCCGGCAGTATGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTc                                                                                                   >  1:515638/1‑140 (MQ=255)                                acacAATAATATTCCGGCAGTATGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCaa                                                                                                 >  2:621233/1‑140 (MQ=255)                                acacAATAATATTCCGGCAGTATGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCaa                                                                                                 >  2:115858/1‑140 (MQ=255)                                  acaATAATATTCCGGCAGTATGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCa                                                                                                         <  2:210608/130‑1 (MQ=255)                                  acaATAATATTCCGGCAGTATGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCaaaa                                                                                               >  2:175499/1‑140 (MQ=255)                                  acaATAATATTCCGGCAGTATGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCaaaa                                                                                               >  1:68620/1‑140 (MQ=255)                                  acaATAATATTCCGGCAGTATGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCaaaa                                                                                               >  1:143380/1‑140 (MQ=255)                                  acaATAATATTCCGGCAGTATGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGACCCATATTGCCGTCAGGCa                                                                                                         >  1:210608/1‑130 (MQ=255)                                   caATAATATTCCGGCAGTATGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCaaaaa                                                                                              >  2:385716/1‑140 (MQ=255)                                            tCCGGCAGTATGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGcc                                                                                     >  2:77900/1‑140 (MQ=255)                                            tCCGGCAGTATGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGcc                                                                                     >  2:44131/1‑140 (MQ=255)                                              cGGCAGTATGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCcac                                                                                   >  2:16124/1‑140 (MQ=255)                                              cGGCAGTATGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGACcac                                                                                   >  1:280983/1‑140 (MQ=255)                                                gCAGTATGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACAt                                                                                 >  2:102067/1‑140 (MQ=255)                                                gCAGTATGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACAt                                                                                 >  2:485707/1‑140 (MQ=255)                                                gCAGTATGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACAt                                                                                 >  2:526588/1‑140 (MQ=255)                                                gCAGTATGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACAt                                                                                 >  1:280510/1‑140 (MQ=255)                                                      tgtgTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGaaa                                                                           >  2:679252/1‑140 (MQ=255)                                                           cGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGtttt                                                                      >  1:252146/1‑140 (MQ=255)                                                              aGCGATATGCGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAAGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCtt                                                                   >  2:473623/1‑140 (MQ=255)                                                                             ttcaatATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTg                                                    >  1:356229/1‑140 (MQ=255)                                                                             ttcaatATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTg                                                    >  2:687497/1‑140 (MQ=255)                                                                                           gTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAgg                                      <  1:44131/140‑1 (MQ=255)                                                                                            tACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGa                                     >  1:185115/1‑140 (MQ=255)                                                                                            tACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGa                                     >  1:636071/1‑140 (MQ=255)                                                                                            tACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCCTGTCATATTTTTGAGGa                                     >  1:676706/1‑140 (MQ=255)                                                                                             acacCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGaa                                    <  1:206765/140‑1 (MQ=255)                                                                                             acacCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGaa                                    <  2:356229/140‑1 (MQ=255)                                                                                             acacCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGaa                                    <  1:16124/140‑1 (MQ=255)                                                                                             acacCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGaa                                    <  2:453016/140‑1 (MQ=255)                                                                                             acacCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCCTGTCATATTTTTGAGGaa                                    <  2:676706/140‑1 (MQ=255)                                                                                              cacCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAAt                                   >  1:670787/1‑140 (MQ=255)                                                                                              cacCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAAt                                   >  1:621336/1‑140 (MQ=255)                                                                                                  ggCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACtt                               <  1:621233/140‑1 (MQ=255)                                                                                                      aGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGc                           >  2:403094/1‑140 (MQ=255)                                                                                                      aGTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGc                           >  2:600057/1‑140 (MQ=255)                                                                                                       gTTGGACTGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGCa                          <  2:714723/140‑1 (MQ=255)                                                                                                              tGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCt                   <  2:280510/140‑1 (MQ=255)                                                                                                              tGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCt                   <  2:670787/140‑1 (MQ=255)                                                                                                              tGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCt                   <  1:509938/140‑1 (MQ=255)                                                                                                              tGCGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCt                   <  1:157898/140‑1 (MQ=255)                                                                                                                cGTAAATAATGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCTGa                 >  2:84546/1‑140 (MQ=255)                                                                                                                        atGAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCTGAGCCCAgcg         >  1:270522/1‑140 (MQ=255)                                                                                                                          gAAACCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCTGAGCCCAGCGCa       >  2:163979/1‑140 (MQ=255)                                                                                                                             aCCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCTGAGCCCAGCGCAGgc    >  2:493204/1‑140 (MQ=255)                                                                                                                             aCCGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCTGAGCCCAGCGCAGgc    <  1:526588/140‑1 (MQ=255)                                                                                                                              ccGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCTGAGCCCAGCGCAGgcg   <  1:403094/140‑1 (MQ=255)                                                                                                                               cGACCAGGAAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCTGAGCCCAGCGCAGgcgc  <  2:616/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                    GGCTGACAATAAATTGCAGTAAACATAACGTACACAATAATATTCCGGCAGTATGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGCAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCTGAGCCCAGCGCAGGCGC  >  NC_000913/1086706‑1086971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑