Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,780,197	C→A	A194E (GCG→GAG)	ydiQ →	putative electron transfer flavoprotein subunit YdiQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,780,197	0	C	A	100.0%	121.5 / NA	36	A194E (GCG→GAG)	ydiQ	putative electron transfer flavoprotein subunit YdiQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (19/17);  total (19/17)

GGCAATACACTGGTGATTGAACGCACGCTTGAAGATGATGTTGAAGTTATTGAACTCTCTGTTCCAGCCGTGCTCTGCGTCACCTCCGATATTAACGTGCCACGCATTCCTTCGATGAAAGCCATTCTCGGCGCGGGTAAAAAACCGGTAAATCAGTGGCAGGCAAGTGATATTGACTGGAGCCAGAGCGCGCCACTTGCTGAACTGGTCGGCATTCGCGTACCGCCGCAAACAGAACGTAAGCACATCATTATCGATAACGATTCGCCG  >  NC_000913/1780064‑1780333                                                                                                                                      |                                                                                                                                         ggCAATACACTGGTGATTGAACGCACGCTTGAAGATGATGTTGAAGTTATTGAACTCTCTGTTCCAGCCGTGCTCTGCGTCACCTCCGATATTAACGTGCCACGCATTCCTTCGATGAAAGCCATTCTCGGCGAGGGTaa                                                                                                                                    >  2:621343/1‑140 (MQ=255)   cAATACACTGGTGATTGAACGCACGCTTGAAGATGATGTTGAAGTTATTGAACTCTCTGTTCCAGCCGTGCTCTGCGTCACCTCCGATATTAACGTGCCACGCATTCCTTCGATGAAAGCCATTCTCGGCGAGGGTaaaa                                                                                                                                  <  2:692420/140‑1 (MQ=255)    aaTACACTGGTGATTGAACGCTCGCTTGAAGATGATGTTGAAGTTATTGAACTCTCAGTTCCAGCCGTGCTCTGCGTCACCTCCGATATTAACGTGCCACGCATTCCTTCGATGAAAGCCATTCTCGGCGAGGGTaaaaa                                                                                                                                 <  2:29586/140‑1 (MQ=255)            ggTGATTGAACGCACGCTTGAAGATGATGTTGAAGTTATTGAACTCTCTGTTCCAGCCGTGCTCTGCGTCACCTCCGATATTAACGTGCCACGCATTCCTTCGATGAAAGCCATTCTCGGCGAGGGTAAAAAACCGGTaa                                                                                                                         >  1:305609/1‑140 (MQ=255)                  tGAACGCACGCTTGAAGATGATGTTGAAGTTATTGAACTCTCTGTTCCAGCCGTGCTCTGCGTCACCTCCGATATTAACGTGCCACGCATTCCTTCGATGAAAGCCATTCTCGGCGAGGGTAAAAAACCGGTAAATCAGt                                                                                                                   >  2:541073/1‑140 (MQ=255)                  tGAACGCACGCTTGAAGATGATGTTGAAGTTATTGAACTCTCTGTTCCAGCCGTGCTCTGCGTCACCTCCGATATTAACGTGCCACGCATTCCTTCGATGAAAGCCATTCTCGGCGAGGGTAAAAAACCGGTAAATCAGt                                                                                                                   >  1:508357/1‑140 (MQ=255)                     acgcacgcTTGAAGATGATGTTGAAGTTATTGAACTCTCTGTTCCAGCCGTGCTCTGCGTCACCTCCGATATTAACGTGCCACGCATTCCTTCGATGAAAGCCATTCTCGGCGAGGGTAAAAAACCGGTAAATCAGTggc                                                                                                                >  2:92481/1‑140 (MQ=255)                       gcacgcTTGAAGATGATGTTGAAGTTATTGAACTCTCTGTTCCAGCCGTGCTCTGCGTCACCTCCGATATTAACGTGCCACGCATTCCTTCGATGAAAGCCATTCTCGGCGAGGGTAAAAAACCGGTAAATCAGTggcag                                                                                                              >  1:258725/1‑140 (MQ=255)                       gcacgcTTGAAGATGATGTTGAAGTTATTGAACTCTCTGTTCCAGCCGTGCTCTGCGTCACCTCCGATATTAACGTGCCACGCATTCCTTCGATGAAAGCCATTCTCGGCGAGGGTAAAAAACCGGTAAATCAGTggcag                                                                                                              >  1:407298/1‑140 (MQ=255)                            cTTGAAGATGATGTTGAAGTTATTGAACTCTCTGTTCCAGCCGTGCTCTGCGTCACCTCCGATATTAACGTGCCACGCATTCCTTCGATGAAAGCCATTCTCGGCGAGGGTAAAAAACCGGTAAATCAGTGGCAGGCAAg                                                                                                         <  1:281067/140‑1 (MQ=255)                            cTTGAAGATGATGTTGAAGTTATTGAACTCTCTGTTCCAGCCGTGCTCTGCGTCACCTCCGATATTAACGTGCCACGCATTCCTTCGATGAAAGCCATTCTCGGCGAGGGTAAAAAACCGGTAAATCAGTGGCAGGCAAg                                                                                                         <  1:370593/140‑1 (MQ=255)                              tGAAGATGATGTTGAAGTTATTGAACTCTCTGTTCCAGCCGTGCTCTGCGTCACCTCCGATATTAACGTGCCACGCATTCCTTCGATGAAAGCCATTCTCGGCGAGGGTAAAAAACCGGTAAATCAGTGGCAGGCAAGTg                                                                                                       <  1:294500/140‑1 (MQ=255)                                      atgTTGAAGTTATTGAACTCTCTGTTCCAGCCGTGCTCTGCGTCACCTCCGATATTAACGTGCCACGCATTCCTTCGATGAAAGCCATTCTCGGCGAGGGTAAAAAACCGGTAAATCAGTGGCAGGCAAGTGATATTGAc                                                                                               <  2:508357/140‑1 (MQ=255)                                      atgTTGAAGTTATTGAACTCTCTGTTCCAGCCGTGCTCTGCGTCACCTCCGATATTAACGTGCCACGCATTCCTTCGATGAAAGCCATTCTCGGCGAGGGTAAAAAACCGGTAAATCAGTGGCAGGCAAGTGATATTGAc                                                                                               <  1:92481/140‑1 (MQ=255)                                       tgTTGAAGTTATTGAACTCTCTGTTCCAGCCGTGCTCTGCGTCACCTCCGATATTAACGTGCCACGCATTCCTTCGATGAAAGCCATTCTCGGCGAGGGTAAAAAACCGGTAAATCAGTGGCAGGCAAGTGATATTGACt                                                                                              >  1:475466/1‑140 (MQ=255)                                       tgTTGAAGTTATTGAACTCTCTGTTCCAGCCGTGCTCTGCGTCACCTCCGATATTAACGTGCCACGCATTCCTTCGATGAAAGCCATTCTCGGCGAGGGTAAAAAACCGGTAAATCAGTGGCAGGCAAGTGATATTGACt                                                                                              >  2:92698/1‑140 (MQ=255)                                          tGAAGTTATTGAACTCTCTGTTCCAGCCGTGCTCTGCGTCACCTCCGATATTAACGTGCCACGCATTCCTTCGATGAAAGCCATTCTCGGCGAGGGTAAAAAACCGGTAAATCAGTGGCAGGCAAGTGATATTGACTGGa                                                                                           >  2:540147/1‑140 (MQ=255)                                                       ctctctGTTCCAGCCGTGCTCTGCGTCACCTCCGATATTAACGTGCCACGCATTCCTTCGATGAAAGCCATTCTCGGCGAGGGTAAAAAACCGGTAAATCAGTGGCAGGCAAGTGATATTGACTGGAGCCAGAGCGCGcc                                                                              <  2:475466/140‑1 (MQ=255)                                                                 cAGCCGTCCTCTGCGTCACCTCCGATATTAACGTGCCACGCATTCCTTCGATGAAAGCCATTCTCGGCGAGGGTAAAAAACCGGTAAATCAGTGGCAGGCAAGTGATATTGACTGGAGCCAGAGCGCGCCACTTGCTGaa                                                                    <  2:210899/140‑1 (MQ=255)                                                                   gCCGTGCTCTGCGTCACCTCCGATATTAACGTGCCACGCATTCCTTCGATGAAAGCCATTCTCGGCGAGGGTAAAAAACCGGTAAATCAGTGGCAGGCAAGTGATATTGACTGGAGCCAGAGCGCGCCACTTGCTGAACt                                                                  >  2:238076/1‑140 (MQ=255)                                                                    ccGTGCTCTGCGTCACCTCCGATATTAACGTGCCACGCATTCCTTCGATGAAAGCCATTCTCGGCGAGGGTAAAAAACCGGTAAATCAGTGGCAGGCAAGTGATATTGACTGGAGCCAGAGCGCGCCACTTGCTGAACTg                                                                 >  1:283020/1‑140 (MQ=255)                                                                              cGTCACCTCCGATATTAACGTGCCACGCATTCCTTCGATGAAAGCCATTCTCGGCGAGGGTAAAAAACCGGTAAATCAGTGGCAGGCAAGTGATATTGACTGGAGCCAGAGCGCGCCACTTGCTGAACTGGTCGGCATTc                                                       >  1:79151/1‑140 (MQ=255)                                                                                     tCCGATATTAACGTGCCACGCATTCCTTCGATGAAAGCCATTCTCGGCGAGGGTAAAAACCCGGTAAATCAGTGGCAGGCAAGTGATATTGACGGGAGCCGGAGCGCGCCACTGGCTGAACTGGTCGGCATTCGCGTAcc                                                <  2:406088/140‑1 (MQ=255)                                                                                      ccGATATTAACGTGCCACGCATTCCTTCGATGAAAGCCATTCTCGGCGAGGGTAAAAAACCGGTAAATCAGTGGCAGGCAAGTGATATTGACTGGAGCCAGAGCGCGCCACTTGCTGAACTGGTCGGCATTCGCGTAccg                                               >  1:635885/1‑140 (MQ=255)                                                                                              aaCGTGCCACGCATTCCTTCGATGAAAGCCATTCTCGGCGAGGGTAAAAAACCGGTAAATCAGTGGCAGGCAAGTGATATTGACTGGAGCCAGAGCGCGCCACTTGCTGAACTGGTCGGCATTCGCGTACCGCCGCAAAc                                       <  2:258725/140‑1 (MQ=255)                                                                                              aaCGTGCCACGCATTCCTACGATGAAAGCCATTCTCGGCGAGGGTAAAAAACCGGTAAATCAGTGGCAGGCAAGTGATATTGACTGGAGCCAGAGCGCGCCACTTGCTGAACTGGTCGGCATTCGCGTACCGCCGCAAAc                                       <  2:166495/140‑1 (MQ=255)                                                                                                     cACGCATTCCTTCGATGAAAGCCATTCTCGGCGAGGGTAAAAAACCGGTAAATCAGTGGCAGGCAAGTGATATTGACTGGAGCCAGAGCGCGCCACTTGCTGAACTGGTCGGCATTCGCGTACCGCCGCAAACAGAACGt                                <  2:549010/140‑1 (MQ=255)                                                                                                     cACGCATTCCTTCGATGAAAGCCATTCTCGGCGAGGGTAAAAAACCGGTAAATCAGTGGCAGGCAAGTGATATTGACTGGAGCCAGAGCGCGCCACTTGCTGAACTGGTCGGCATTCGCGTACCGCCGCAAACAGAACGt                                <  1:621343/140‑1 (MQ=255)                                                                                                     cACGCATTCCTTCGATGAAAGCCATTCTCGGCGAGGGTAAAAAACCGGTAAATCAGTGGCAGGCAAGTGATATTGACTGGAGCCAGAGCGCGCCACTTGCTGAACTGGTCGGAATTCGCGTACCGCCGCAAACAGAACGt                                <  2:305609/140‑1 (MQ=255)                                                                                                      aCGCATTCCTTCGATGAAAGCCATTCTCGGCGAGGGTAAAAAACCGGTAAATCAGTGGCAGGCAAGTGATATTGACTGGAGCCAGAGCGCGCCACTTGCTGAACTGGTCGGCATTCGCGTACCGCCGCAAACAGAACGTa                               >  1:689756/1‑140 (MQ=255)                                                                                                        gCATTCCTTCGATGAAAGCCATTCTCGGCGAGGGTAAAAAACCGGAAAATCAGTGGCAGGTAAGTGATATTGACTGGAGCCAGAGCGCGCCACTTGCTGAACTGGTCGGCATTCGCGTACCGCCGCAAACAGAACGTAAg                             <  2:225068/140‑1 (MQ=255)                                                                                                          aTTCCTTCGATGAAAGCCATTCTCGGCGAGGGTAAAAAACCGGTAAATCAGTGGCAGGCAAGTGATATTGACTGGAGCCAGAGCGCGCCACTTGCTGAACTGGTCGGCATTCGCGTACCGCCGCAAACAGAACGTAAGca                           >  2:505854/1‑140 (MQ=255)                                                                                                              cTTCGATGAAAGCCATTCTCGGCGAGGGTAAAAAACCGGTAAATCAGTGGCAGGCAAGTGATATTGACTGGAGCCAGAGCGCGCCACTTGCTGAACTGGTCGGCATTCGCGTACCGCCGCAAACAGAACGTAAGCAcatc                       >  1:255717/1‑140 (MQ=255)                                                                                                               ttCGATGAAAGCCATTCTCGGCGAGGGTAAAAAACCGGTAAATCAGTGGCAGGCAAGTGATATTGACTGGAGCCAGAGCGCGCCACTTGCTGAACTGGTCGGCATTCGCGTACCGCCGCAAACAGAACGTAAGCAcatca                      <  2:45333/140‑1 (MQ=255)                                                                                                                 cGATGAAAGCCATTCTCGGCGAGGGTAAAAAACCGGTAAATCAGTGGCAGGCAAGTGATATTGACTGGAGCCAGAGCGCGCCACTTGCTGAACTGGTCGGCATTCGCGTACCGCCGCAAACAGAACGTAAGCACATCAtt                    >  1:265392/1‑140 (MQ=255)                                                                                                                        aGCCATTCTCGGCGAGGGTAAAAAACCGGTAAATCAGTGGCAGGCAAGTGATATTGACTGGAGCCAGAGCGCGCCACTTGCTGAACTGGTCGGCATTCGCGTACCGCCGCAAACAGAACGTAAGCACATCATTATCGATa             >  2:9887/1‑140 (MQ=255)                                                                                                                              tctcGGCGAGGGTAAAAAACCGGTAAATCAGTGGCAGGCAAGTGATATTGACTGGAGCCAGAGCGCGCCACTTGCTGAACTGGTCGGCATTCGCGTACCGCCGCAAACAGAACGTAAGCACATCATTATCGATAACGAtt       <  2:255717/140‑1 (MQ=255)                                                                                                                              tcccggcggGGGTAAAAAACCGGTAAATCAGTGGCAGGCAAGGGATATTGACTGGAGCCAGAGCGCGCCACTTGCTGAACTGGTCGGCATTCGCGTACCGCCGCAAACAGAACGTAAGCACATCATTATCGATAACGAtt       <  2:379448/137‑1 (MQ=255)                                                                                                                                   gcgaGGGTAAAAAATCGGTAAATCAGTGGCAGGCAAGTGATATTGACTGGAGCCAGAGCGCGCCACTTGCTGAACTGGTCGGCATTCGCGTACCGCCGCAAACAGAACGTAAGCACATCATTATCGATAACGATTCGCCg  <  1:623727/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         GGCAATACACTGGTGATTGAACGCACGCTTGAAGATGATGTTGAAGTTATTGAACTCTCTGTTCCAGCCGTGCTCTGCGTCACCTCCGATATTAACGTGCCACGCATTCCTTCGATGAAAGCCATTCTCGGCGCGGGTAAAAAACCGGTAAATCAGTGGCAGGCAAGTGATATTGACTGGAGCCAGAGCGCGCCACTTGCTGAACTGGTCGGCATTCGCGTACCGCCGCAAACAGAACGTAAGCACATCATTATCGATAACGATTCGCCG  >  NC_000913/1780064‑1780333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑