Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,244,751	C→A	intergenic (+5/+27)	yeiG → / ← cirA	S‑formylglutathione hydrolase/S‑lactoylglutathione hydrolase/ferric dihyroxybenzoylserine outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,244,751	0	C	A	100.0%	178.2 / NA	51	intergenic (+5/+27)	yeiG/cirA	S‑formylglutathione hydrolase/S‑lactoylglutathione hydrolase/ferric dihyroxybenzoylserine outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (35/16);  total (35/16)

CGCGCCAGAAAGCGTGGCCGATGACGCTGCGTATTCAGCCGGGATATGATCACAGTTACTACTTCATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGC  >  NC_000913/2244625‑2244881                                                                                                                               |                                                                                                                                   cgcgcCAGAAAGCGTGGCCGATGACGCTGCGTATTCAGCCGGGATATGATCACAGTTACTACTTCATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTACGCCCGGTTCGcc                                                                                                                       >  2:308256/1‑140 (MQ=255)                  ccGATGACGCTGCGTATTCAGCCGGGATATGATCACAGTTACTACTTCATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAg                                                                                                      >  2:982521/1‑140 (MQ=255)                   cGATGACGCTGCGTATTCAGCCGGGATATGATCACAGTTACTACTTCATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGa                                                                                                     <  2:171416/140‑1 (MQ=255)                   cGATGACGCTGCGTATTCAGCCGGGATATGATCACAGTTACTACTTCATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGa                                                                                                     <  1:932407/140‑1 (MQ=255)                     aTGACGCTGCGTATTCAGCCGGGATATGATCACAGTTACTACTTCATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAg                                                                                                   <  1:253483/140‑1 (MQ=255)                     aTGACGCTGCGTATTCAGCCGGGATATGATCACAGTTACTACTTCATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAg                                                                                                   <  1:308256/140‑1 (MQ=255)                                               tGATCACAGTTACTACTTCATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAacg                                                                         >  1:215385/1‑140 (MQ=255)                                                gATCACAGTTACTACTTCATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAacga                                                                        <  1:982521/140‑1 (MQ=255)                                                     caGTTACTACTTCATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGAcgtcc                                                                   >  2:881225/1‑140 (MQ=255)                                                         tactacTTCATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCt                                                               >  2:2114/1‑140 (MQ=255)                                                          actactTCATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCtt                                                              <  1:2114/140‑1 (MQ=255)                                                                tCATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTtat                                                        >  2:254271/1‑140 (MQ=255)                                                                tCATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTtat                                                        >  1:666289/1‑140 (MQ=255)                                                                tCATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTtat                                                        >  2:495286/1‑140 (MQ=255)                                                                   tCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGc                                                     >  2:1030315/1‑140 (MQ=255)                                                                      ccTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGt                                                  >  2:392377/1‑140 (MQ=255)                                                                       ctctTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTa                                                 <  2:800953/140‑1 (MQ=255)                                                                        tctTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAg                                                >  2:1017473/1‑140 (MQ=255)                                                                        tctTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAg                                                >  1:785987/1‑140 (MQ=255)                                                                         ctTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAgt                                               <  1:255347/140‑1 (MQ=255)                                                                         ctTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAgt                                               >  1:190427/1‑140 (MQ=255)                                                                                   ggATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACt                                     <  1:642354/140‑1 (MQ=255)                                                                                   ggATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACt                                     <  1:589240/140‑1 (MQ=255)                                                                                    gATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTg                                    >  2:763138/1‑140 (MQ=255)                                                                                      tCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAgtcgt                                            >  1:896299/1‑130 (MQ=255)                                                                                      tCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAgtcgt                                            <  2:896299/130‑1 (MQ=255)                                                                                            gcgcTTCCATGCGCAGTATTTACTGAAGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTgaga                                 <  1:229617/135‑1 (MQ=255)                                                                                            gcgcTTCCATGCGCAGTATTTACTGAAGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTgaga                                 >  2:229617/1‑135 (MQ=255)                                                                                            gcgcTTCCATGCGCAGTATTTACTGAAGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCtt                            >  1:590402/1‑140 (MQ=255)                                                                                            gcgcTTCCATGCGCAGTATTTACTGAAGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCtt                            >  2:14792/1‑140 (MQ=255)                                                                                              gcTTCCATGCGCAGTATTTACTGAAGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGt                          >  1:959428/1‑140 (MQ=255)                                                                                              gcTTCCATGCGCAGTATTTACTGAAGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGt                          >  1:22128/1‑140 (MQ=255)                                                                                                 tCCATGCGCAGTATTTACTGAAGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGc                       <  1:392377/140‑1 (MQ=255)                                                                                                  ccATGCGCAGTATTTACTGAAGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGcc                      >  2:380644/1‑140 (MQ=255)                                                                                                       cgcAGTATTTACTGAAGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGt                 >  1:945897/1‑140 (MQ=255)                                                                                                            tATTTACTGAAGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGc            >  2:275125/1‑140 (MQ=255)                                                                                                            tATTTACTGAAGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGc            >  1:198459/1‑140 (MQ=255)                                                                                                            tATTTACTGAAGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGc            >  1:148857/1‑140 (MQ=255)                                                                                                             aTTTACTGAAGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCa           <  1:477692/140‑1 (MQ=255)                                                                                                                      aGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGGGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTgcg   <  2:539776/139‑1 (MQ=255)                                                                                                                      aGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGGGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTgcg   >  1:539776/1‑139 (MQ=255)                                                                                                                      aGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCa           >  1:559709/1‑131 (MQ=255)                                                                                                                      aGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCa           <  2:559709/131‑1 (MQ=255)                                                                                                                      aGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTgcgc  >  1:1050009/1‑140 (MQ=255)                                                                                                                      aGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTgcgc  >  1:893622/1‑140 (MQ=255)                                                                                                                      aGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTgcgc  >  1:685498/1‑140 (MQ=255)                                                                                                                      aGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTgcgc  >  2:419622/1‑140 (MQ=255)                                                                                                                      aGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTgcgc  >  2:423320/1‑140 (MQ=255)                                                                                                                      aGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTgcgc  >  2:6549/1‑140 (MQ=255)                                                                                                                      aGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTgcgc  >  2:85999/1‑140 (MQ=255)                                                                                                                      aGTGAAAGTACGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTgcgc  >  1:308866/1‑140 (MQ=255)                                                                                                                               |                                                                                                                                   CGCGCCAGAAAGCGTGGCCGATGACGCTGCGTATTCAGCCGGGATATGATCACAGTTACTACTTCATCGCCTCTTTTATAGAGGATCACCTGCGCTTCCATGCGCAGTATTTACTGAAGTGAAAGTCCGCCCGGTTCGCCGGGCATCTTCTCATCAGAAGCGATAATCCACTGCCATAAAGTAACGACGTCCGTCTTCGTTATAGCTGTAGTCGTCACGACTGAGATCCTTGTCGCCAAGGTTCAGCACGCCTGCGC  >  NC_000913/2244625‑2244881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑