Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,316,150 G→T S888I (AGC→ATC)  rcsD → RcsD phosphotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,316,1500GT97.7% 304.8 / 0.2 88S888I (AGC→ATC) rcsDRcsD phosphotransferase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (38/48);  total (39/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

CGTCTTAAAGGCGTATTTGCCATGCTAAATCTGGTACCCGGCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGAGCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGA  >  NC_000913/2316014‑2316286
                                                                                                                                        |                                                                                                                                        
cGTCTTAAAGGCGTATTTGCCATGCTAAATCTGGTACCCGGCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCtt                                                                                                                                       >  1:841061/1‑140 (MQ=255)
   cTTAAAGGCGTATTTGCCATGCTAAATCTGGTACCCGGCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCt                                                                                                                                    >  1:8973/1‑140 (MQ=255)
       aaGGCGTATTTGCCATGCTAAATCTGGTACCCGGCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAg                                                                                                                                <  2:844687/140‑1 (MQ=255)
       aaGGCGTATTTGCCATGCTAAATCTGGTACCCGGCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAg                                                                                                                                <  2:849893/140‑1 (MQ=255)
        aGGCGTATTTGCCATGCTAAATCTGGTACCCGGCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGc                                                                                                                               <  1:434049/140‑1 (MQ=255)
          gCGTATTTGCCATGCTAAATCTGGTACCCGGCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCaa                                                                                                                             >  2:132228/1‑140 (MQ=255)
          gCGTATTTGCCATGCTAAATCTGGTACCCGGCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCaa                                                                                                                             >  1:462010/1‑140 (MQ=255)
           cGTATTTGCCATGCTAAATCTGGTACCCGGCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAg                                                                                                                            <  2:150057/140‑1 (MQ=255)
           cGTATTTGCCATGCTAAATCTGGTACCCGGCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAg                                                                                                                            <  2:841632/140‑1 (MQ=255)
           cGTATTTGCCATGCTAAATCTGGTACCCGGCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAg                                                                                                                            >  2:587848/1‑140 (MQ=255)
           cGTATTTGCCATGCTAAATCTGGTACCCGGCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAg                                                                                                                            <  2:630132/140‑1 (MQ=255)
            gTATTTGCCATGCTAAATCTGGTACCCGGCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAgg                                                                                                                           <  1:871487/140‑1 (MQ=255)
            gTATTTGCCATGCTAAATCTGGTACCCGGCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAgg                                                                                                                           <  1:132228/140‑1 (MQ=255)
             tATTTGCCATGCTAAATCTGGTACCCGGCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGt                                                                                                                          >  2:327387/1‑140 (MQ=255)
             tATTTGCCATGCTAAATCTGGTACCCGGCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGt                                                                                                                          >  2:906237/1‑140 (MQ=255)
             tATTTGCCATGCTAAATCTGGTACCCGGCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGt                                                                                                                          >  2:434636/1‑140 (MQ=255)
             tATTTGCCATGCTAAATCTGGTACCCGGCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGt                                                                                                                          >  2:489847/1‑140 (MQ=255)
             tATTTGCCATGCTAAATCTGGTACCCGGCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGt                                                                                                                          >  1:981467/1‑140 (MQ=255)
             tATTTGCCATGCTAAATCTGGTACCCGGCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGt                                                                                                                          >  1:720237/1‑140 (MQ=255)
              aTTTGCCATGCTAAATCTGGTACCCGGCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTa                                                                                                                         <  2:981467/140‑1 (MQ=255)
              aTTTGCCATGCTAAATCTGGTACCCGGCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTa                                                                                                                         <  1:681507/140‑1 (MQ=255)
              aTTTGCCATGCTAAATCTGGTACCCGGCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTa                                                                                                                         <  2:654543/140‑1 (MQ=255)
                  gCCATGCTAAATCTGGTACCCGGCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCt                                                                                                                     <  2:339547/140‑1 (MQ=255)
                  gCCATGCTAAATCTGGTACCCGGCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCt                                                                                                                     <  1:444956/140‑1 (MQ=255)
                      tGCTAAATCTGGTACCCGGCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTa                                                                                                                 >  2:60963/1‑140 (MQ=255)
                       gCTAAATCTGGTACCCGGCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGAGCTTGCTGTAGCAAGGTAGCCTATTAc                                                                                                                >  2:598789/1‑140 (MQ=255)
                        cTAAATCTGGTACCCGGCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACa                                                                                                               <  2:446502/140‑1 (MQ=255)
                         tAAATCTGGTACCCGGCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGAGCTTGCTGTAGCAAGGTAGCCTATTACAt                                                                                                              <  1:598789/140‑1 (MQ=255)
                          aaaTCTGGTACCCGGCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATg                                                                                                             <  1:609551/140‑1 (MQ=255)
                           aaTCTGGTACCCGGCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGa                                                                                                            <  2:8973/140‑1 (MQ=255)
                                ggTACCCGGCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAat                                                                                                       <  1:409239/140‑1 (MQ=255)
                                 gTACCCGGCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAata                                                                                                      >  2:604885/1‑140 (MQ=255)
                                 gTACCCGGCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAata                                                                                                      <  1:489262/140‑1 (MQ=255)
                                  tACCCGGCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAatat                                                                                                     >  2:155349/1‑140 (MQ=255)
                                     ccGGCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGaa                                                                                                  >  1:687288/1‑140 (MQ=255)
                                       ggCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAatat                                                                                                     >  1:38191/1‑135 (MQ=255)
                                       ggCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAatat                                                                                                     <  2:38191/135‑1 (MQ=255)
                                       ggCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACg                                                                                                <  1:598706/140‑1 (MQ=255)
                                        gCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGt                                                                                               >  1:1047821/1‑140 (MQ=255)
                                         cAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTa                                                                                              >  2:317665/1‑140 (MQ=255)
                                             cAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAatta                                                                                          <  2:147361/140‑1 (MQ=255)
                                             cAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAatta                                                                                          <  1:327387/140‑1 (MQ=255)
                                                     tgAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGAt                                                                                  <  2:462010/140‑1 (MQ=255)
                                                       aaaCGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGa                                                                                >  1:567581/1‑140 (MQ=255)
                                                       aaaCGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAACATGAACGTAATTATTGCCGATGa                                                                                <  1:368695/140‑1 (MQ=255)
                                                          cGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCa                                                                             <  2:841061/140‑1 (MQ=255)
                                                          cGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCa                                                                             <  2:720237/140‑1 (MQ=255)
                                                          cGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCa                                                                             >  2:608286/1‑140 (MQ=255)
                                                            cTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATc                                                                           <  1:626088/140‑1 (MQ=255)
                                                               gAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGa                                                                        <  1:104273/140‑1 (MQ=255)
                                                               gAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGa                                                                        <  2:1047821/140‑1 (MQ=255)
                                                                 aCATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATa                                                                      <  2:489059/140‑1 (MQ=255)
                                                                    tCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACATAATTATTGCCGATGACCATCCGATAGTc                                                                   >  2:905435/1‑140 (MQ=255)
                                                                     cTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCt                                                                  <  2:946625/140‑1 (MQ=255)
                                                                     cTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCt                                                                  >  1:29878/1‑140 (MQ=255)
                                                                       gATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTg                                                                <  1:489847/140‑1 (MQ=255)
                                                                               agaAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTAt                                                        >  1:80535/1‑140 (MQ=255)
                                                                                      tGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCaaa                                                 >  1:706324/1‑140 (MQ=255)
                                                                                       gTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAAt                                                <  1:587848/140‑1 (MQ=255)
                                                                                         tCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCa                                              <  1:906237/140‑1 (MQ=255)
                                                                                         tCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCa                                              <  2:687288/140‑1 (MQ=255)
                                                                                             ggAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTg                                          <  1:155349/140‑1 (MQ=255)
                                                                                              gAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGa                                         >  2:20761/1‑140 (MQ=255)
                                                                                               aaTAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAg                                        <  1:20761/140‑1 (MQ=255)
                                                                                               aaTAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAg                                        <  1:392855/140‑1 (MQ=255)
                                                                                               aaTAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAg                                        <  1:177171/140‑1 (MQ=255)
                                                                                                 tAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCa                                      <  1:434636/140‑1 (MQ=255)
                                                                                                  aGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGTACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCaa                                     >  1:547852/1‑140 (MQ=255)
                                                                                                  aGAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCaa                                     >  1:938701/1‑140 (MQ=255)
                                                                                                   gAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCaaa                                    <  2:390495/140‑1 (MQ=255)
                                                                                                   gAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCaaa                                    >  1:71660/1‑140 (MQ=255)
                                                                                                   gAAAAATACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCaaa                                    >  1:926021/1‑140 (MQ=255)
                                                                                                    aaaaaTACATCAGCGACA‑TGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAAtt                                  >  1:1017342/1‑140 (MQ=255)
                                                                                                       aaTACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGa                                <  1:604885/140‑1 (MQ=255)
                                                                                                        aTACATCAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGTACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAg                               <  2:547852/140‑1 (MQ=255)
                                                                                                              cAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTg                         >  2:167430/1‑140 (MQ=255)
                                                                                                              cAGCGACATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTg                         >  2:414109/1‑140 (MQ=255)
                                                                                                                   aCATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAg                               <  1:749304/129‑1 (MQ=255)
                                                                                                                   aCATTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAg                               >  2:749304/1‑129 (MQ=255)
                                                                                                                     aTTGACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAAtgttg                  >  1:948679/1‑140 (MQ=255)
                                                                                                                        gACAGTTATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAG‑GGGTGAATGTTGTCgg              <  1:865470/140‑1 (MQ=255)
                                                                                                                             ttATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGaa          >  2:735634/1‑140 (MQ=255)
                                                                                                                              tATGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAAt         >  2:1015150/1‑140 (MQ=255)
                                                                                                                                tGTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAAttt       >  1:816330/1‑140 (MQ=255)
                                                                                                                                cgTCAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAAttt       >  2:681296/2‑140 (MQ=255)
                                                                                                                                  tCAAGATCTTGCTGTAGCAAGGCAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGa     <  1:2285/140‑1 (MQ=255)
                                                                                                                                   cAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGaa    <  2:706324/140‑1 (MQ=255)
                                                                                                                                   cAAGATCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGaa    <  1:735634/140‑1 (MQ=255)
                                                                                                                                     agatCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGa  <  2:80535/140‑1 (MQ=255)
                                                                                                                                     agatCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGa  <  1:863768/140‑1 (MQ=255)
                                                                                                                                     agatCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGa  <  1:167430/140‑1 (MQ=255)
                                                                                                                                     agatCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGa  <  2:781315/140‑1 (MQ=255)
                                                                                                                                     agatCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGa  <  2:948679/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
CGTCTTAAAGGCGTATTTGCCATGCTAAATCTGGTACCCGGCAAGCAGTTATGTGAAACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGAGCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGA  >  NC_000913/2316014‑2316286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑