Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,436,238	G→T	L138M (CTG→ATG)	usg ←	putative semialdehyde dehydrogenase Usg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,436,238	0	G	T	100.0%	144.1 / NA	41	L138M (CTG→ATG)	usg	putative semialdehyde dehydrogenase Usg
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (20/21);  total (20/21)

CCGTTGAGCAATTTCGCACTCTGCCCCGCTAACGCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGT  >  NC_000913/2436105‑2436366                                                                                                                                      |                                                                                                                                 ccGTTGAGCAATTTCGCACTCTGCCCCGCTAACGCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCATCAgctg                                                                                                                            <  2:275647/140‑1 (MQ=255) ccGTTGAGCAATTTCGCACTCTGCCCCGCTAACGCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCATCAgctg                                                                                                                            >  1:923146/1‑140 (MQ=255)  cGTTGAGCAATTTCGCACTCTGCCCCGCTAACGCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCATCAgctgg                                                                                                                           >  2:158592/1‑140 (MQ=255)  cGTTGAGCAATTTCGCACTCTGCCCCGCTAACGCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCATCAgctgg                                                                                                                           <  2:761530/140‑1 (MQ=255)  cGTTGAGCAATTTCGCACTCTGCCCCGCTAACGCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCATCAgctgg                                                                                                                           <  2:1028129/140‑1 (MQ=255)       aGCAATTTCGCACTCTGCCCCGCTAACGCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAGCCGCCCTGATCGATTAACGGTTTCAGTGCCGCCATCAgctg                                                                                                                            <  2:536295/134‑1 (MQ=255)       aGCAATTTCGCACTCTGCCCCGCTAACGCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCATCAgctg                                                                                                                            >  1:536295/1‑134 (MQ=255)       aGCAATTTCGCACTCTGCCCCGCTAACGCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCATCAGCTGGCTGGt                                                                                                                      >  1:527954/1‑140 (MQ=255)           aTTTCGCACTCTGCCCCGCTAACGCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCATCAGCTGGCTGGTCAGa                                                                                                                  <  2:146727/140‑1 (MQ=255)           aTTTCGCACTCTGCCCCGCTAACGCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCATCAGCTGGCTGGTCAGa                                                                                                                  <  2:996808/140‑1 (MQ=255)           aTTTCGCACTCTGCCCCGCTAACGCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCATCAGCTGGCTGGTCAGa                                                                                                                  <  2:398887/140‑1 (MQ=255)           aTTTCGCACTCTGCCCCGCTAACGCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCATCAGCTGGCTGGTCAGa                                                                                                                  <  2:411338/140‑1 (MQ=255)                    tctGCCCCGCTAACGCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCATCAGCTGGCTGGTCAGACTGTCTGGt                                                                                                         <  1:1032041/140‑1 (MQ=255)                        ccccGCTAACGCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCATCAGCTGGCTGGTCAGACTGTCTGGTACgg                                                                                                     <  1:422783/140‑1 (MQ=255)                                      cGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCATCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACAttccgg                                                                                       <  1:546796/140‑1 (MQ=255)                                      cGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCATCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACAttccgg                                                                                       >  2:254884/1‑140 (MQ=255)                                       gACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCATCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACAttccggt                                                                                      >  2:532077/1‑140 (MQ=255)                                        aCCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCATCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACAttccggtt                                                                                     >  1:748279/1‑140 (MQ=255)                                          cGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCATCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACAttccggttcc                                                                                   <  2:1035586/140‑1 (MQ=255)                                               ttttGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCATCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTaa                                                                              <  2:562289/140‑1 (MQ=255)                                               ttttGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCATCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTaa                                                                              <  1:158592/140‑1 (MQ=255)                                                  tGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCATCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCt                                                                           <  2:999081/140‑1 (MQ=255)                                                                   gAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCATCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGtt                                                          >  1:961538/1‑140 (MQ=255)                                                                   gAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCATCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGtt                                                          >  2:914468/1‑140 (MQ=255)                                                                          ggCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCATCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTcc                                                   >  2:128532/1‑140 (MQ=255)                                                                          ggCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCATCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTcc                                                   >  1:645002/1‑140 (MQ=255)                                                                            cTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCATCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCgg                                                 <  1:128532/140‑1 (MQ=255)                                                                            cTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCATCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCgg                                                 >  2:719208/1‑140 (MQ=255)                                                                                    gCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCATCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCAccacca                                         <  1:247842/140‑1 (MQ=255)                                                                                        aTACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCATCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCgg                                     <  1:55490/140‑1 (MQ=255)                                                                                         tACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCATCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGt                                    >  1:489560/1‑140 (MQ=255)                                                                                          aCGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCATCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTa                                   <  2:923146/140‑1 (MQ=255)                                                                                                   aCCGCCCTGATCGATTAACGGTTTCAGTGCCGCCATCAGCTGGCTGGACAGACTGTCTGGCACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGtt                          >  2:984865/1‑140 (MQ=255)                                                                                                      gCCCTGATCGATTAACGGTTTCAGTGCCGCCATCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCga                       >  1:870339/1‑140 (MQ=255)                                                                                                       cccTGATCGATTAACGGTTTCAGTGCCGCCATCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCgag                      >  2:149114/1‑140 (MQ=255)                                                                                                            aTCGATTAACGGTTTCAGTGCCGCCATCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCaa                 >  2:699285/1‑140 (MQ=255)                                                                                                                  tAACGGTTTCAGTGCCGCCATCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATc           <  2:527954/140‑1 (MQ=255)                                                                                                                    aCGGTTTCAGTGCCGCCATCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCa         <  2:645002/140‑1 (MQ=255)                                                                                                                          tCAGTGCCGCCATCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCActgctg   >  1:243994/1‑140 (MQ=255)                                                                                                                           cAGTGCCGCCATCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGt  >  2:560497/1‑140 (MQ=255)                                                                                                                           cAGTGCCGCCATCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGt  >  1:48933/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                 CCGTTGAGCAATTTCGCACTCTGCCCCGCTAACGCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGT  >  NC_000913/2436105‑2436366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑