Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,448,272	G→T	L57M (CTG→ATG)	prmB ←	50S ribosomal subunit protein L3 N(5)‑glutamine methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,448,272	0	G	T	100.0%	182.6 / NA	53	L57M (CTG→ATG)	prmB	50S ribosomal subunit protein L3 N(5)‑glutamine methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (24/29);  total (24/29)

TGGCCGCAGAACCACGCTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCT  >  NC_000913/2448136‑2448406                                                                                                                                         |                                                                                                                                       tGGCCGCAGAACCACGCTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCgg                                                                                                                                     <  1:915260/140‑1 (MQ=255) tGGCCGCAGAACCACGCTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCgg                                                                                                                                     <  2:755109/140‑1 (MQ=255)  ggCCGCAGAACCACGCTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCGGc                                                                                                                                    >  2:299851/1‑140 (MQ=255)      gCAGAACCACGCTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCGGCAGGt                                                                                                                                <  2:849536/140‑1 (MQ=255)            ccACGCTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCg                                                                                                                          <  2:478173/140‑1 (MQ=255)             cACGCTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGa                                                                                                                         <  1:380431/140‑1 (MQ=255)               cGCTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAg                                                                                                                       >  1:716901/1‑140 (MQ=255)                    tgttgGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAGGCAac                                                                                                                  <  1:299851/140‑1 (MQ=255)                    tgttgGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAGGCAac                                                                                                                  >  1:518299/1‑140 (MQ=255)                       tgGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAGGCAACAcc                                                                                                               <  1:911338/140‑1 (MQ=255)                           cAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAGGCAACAcc                                                                                                               <  2:155543/136‑1 (MQ=255)                           cAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAGGCAACAcc                                                                                                               >  1:155543/1‑136 (MQ=255)                            aGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAGGCAACACCAGCTg                                                                                                          >  1:141199/1‑140 (MQ=255)                            aGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAGGCAACACCAACTg                                                                                                          >  2:735145/1‑140 (MQ=255)                              gTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAGGCAACACCAGCTGTa                                                                                                        >  1:580376/1‑140 (MQ=255)                                        ggAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATc                                                                                              >  1:830926/1‑140 (MQ=255)                                         gAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATcc                                                                                             <  2:716901/140‑1 (MQ=255)                                             gCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATACATCGGCAGGGAGAGCGGAGGGAACACCAGCTGTACGGCTTCATCCCACg                                                                                         >  2:308210/1‑140 (MQ=255)                                              cGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACgg                                                                                        <  2:655335/140‑1 (MQ=255)                                                ttCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGt                                                                                      <  2:62866/140‑1 (MQ=255)                                                ttCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGt                                                                                      <  2:141199/140‑1 (MQ=255)                                                     tGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCg                                                                                 <  2:241977/140‑1 (MQ=255)                                                          cggcggATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTAcc                                                                            >  1:624110/1‑140 (MQ=255)                                                              ggATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGa                                                                        <  1:617924/140‑1 (MQ=255)                                                               gATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGAc                                                                       <  1:1058654/140‑1 (MQ=255)                                                                     gcgTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTAcc                                                                 <  1:84987/140‑1 (MQ=255)                                                                              aaTACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTg                                                        <  1:1022304/140‑1 (MQ=255)                                                                               aTACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGc                                                       >  1:386989/1‑140 (MQ=255)                                                                                 aCGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCcg                                                     >  2:420990/1‑140 (MQ=255)                                                                                  cGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCcgc                                                    <  2:185601/140‑1 (MQ=255)                                                                                   ggTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCcgcg                                                   >  1:78009/1‑140 (MQ=255)                                                                                     tgtTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCt                                                 >  1:222121/1‑140 (MQ=255)                                                                                     tgtTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCGGCAGGCAGAGCGAAGGCAACACCAGCTGTACGGCATCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCgcgcc                                                 >  2:665243/1‑139 (MQ=255)                                                                                      gtTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATACATCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTg                                                <  1:308210/140‑1 (MQ=255)                                                                                               tGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAGGGAACACCAGCTGTACCGCTTCATCTCACGGGCTATCGGCACCCCGACCGGATCAGATATTTTCCGCGCTGCAGCGGCTc                                       >  2:523527/1‑140 (MQ=255)                                                                                               tGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCGGCAGGGAGGGCGCAGGCAACACCAGCTGGACGGCTTCAACCCCCCGGGGATCGGTACCGTGAACGGACCAAAAATTTTTCGCGCCGAAGCGGcgc                                       >  2:792367/1‑138 (MQ=255)                                                                                                ggAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCa                                      <  1:1026095/140‑1 (MQ=255)                                                                                                 gAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCg                                           <  1:251693/134‑1 (MQ=255)                                                                                                 gAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAGGCAAAACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCg                                           >  2:251693/1‑134 (MQ=255)                                                                                                     tCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGAc                                 <  2:222121/140‑1 (MQ=255)                                                                                                     tCAGACGCGCGGTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGAc                                 <  2:624110/140‑1 (MQ=255)                                                                                                         aCGCGCGGTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAgc                             >  2:412913/1‑140 (MQ=255)                                                                                                         aCGCGCGGTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAgc                             <  1:750894/140‑1 (MQ=255)                                                                                                              cgGTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAAc                        <  1:420990/140‑1 (MQ=255)                                                                                                              cgGTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAAc                        <  2:830926/140‑1 (MQ=255)                                                                                                                gTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACAt                      <  1:412913/140‑1 (MQ=255)                                                                                                                gTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACAt                      >  1:34092/1‑140 (MQ=255)                                                                                                                gTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACAt                      <  1:229782/140‑1 (MQ=255)                                                                                                                gTGCGCATATCTTCCGGAATATCCATCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACAt                      <  1:192368/140‑1 (MQ=255)                                                                                                                   cgcATATCTTCCGGAATATCCATCGGCAGGGAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGGGCCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTc                   >  2:938242/1‑140 (MQ=255)                                                                                                                           ttCCGGAATATCCATCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATgg           >  1:450701/1‑140 (MQ=255)                                                                                                                             ccGGAATATCCATCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGGTCACCGACCAGCGCAACATGTCCTGAATGGtt         >  1:776340/1‑140 (MQ=255)                                                                                                                                    atCCATCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCt  >  1:824951/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       TGGCCGCAGAACCACGCTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCT  >  NC_000913/2448136‑2448406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑