Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	708,647	G→T	S105I (AGC→ATC)	chiP →	chitobiose outer membrane channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	708,647	0	G	T	100.0%	162.1 / NA	47	S105I (AGC→ATC)	chiP	chitobiose outer membrane channel
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (18/29);  total (18/29)

CGGCGACAAATACAAAACCAACCTTTCTCACTCCACCTGGAATGCCAACCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCGAGGGC  >  NC_000913/708510‑708779                                                                                                                                          |                                                                                                                                     cGGCGACAAATACAAAACCAACCTTTCTCACTCCACCTGGAATGCCAACCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCt                                                                                                                                    >  1:924081/1‑140 (MQ=255)   gCGACAAATACAAAACCAACCTTTCTCACTCCACCTGGAATGCCAACCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCTcc                                                                                                                                  >  2:269818/1‑140 (MQ=255)                     accTTTCTCACTCCACCTGGAATGCCAACCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATcgcg                                                                                                                >  1:573827/1‑140 (MQ=255)                          tctcACTCCACCTGGAATGCCAACCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTc                                                                                                           <  1:400921/140‑1 (MQ=255)                           ctcACTCCACCTGGAATGCCAACCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCa                                                                                                          <  1:606508/140‑1 (MQ=255)                           ctcACTCCACCTGGAATGCCAACCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCa                                                                                                          <  2:573827/140‑1 (MQ=255)                            tcACTCCACCTGGAATGCCAACCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCaa                                                                                                         >  1:13014/1‑140 (MQ=255)                             cACTCCACCTGGAATGCCAACCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCaaa                                                                                                        <  1:880361/140‑1 (MQ=255)                               cTCCACCTGGAATGCCAACCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCaaaaa                                                                                                      <  2:384971/140‑1 (MQ=255)                                tCCACCTGGAATGCCAACCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAg                                                                                                     >  2:536981/1‑140 (MQ=255)                                tCCACCTGGAATGCCAACCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAg                                                                                                     >  2:248334/1‑140 (MQ=255)                                tCCACCTGGAATGCCAACCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAg                                                                                                     >  1:60977/1‑140 (MQ=255)                                    ccTGGAATGCCAACCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGtaat                                                                                                 >  2:1025646/1‑140 (MQ=255)                                    ccTGGAATGCCAACCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGtaat                                                                                                 <  2:800929/140‑1 (MQ=255)                                    ccTGGAATGCCAACCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGtaat                                                                                                 >  1:908754/1‑140 (MQ=255)                                        gAATGCCAACCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAg                                                                                             >  2:77726/1‑140 (MQ=255)                                          aTGCCAACCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGcc                                                                                           >  1:570660/1‑140 (MQ=255)                                           tGCCAACCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCt                                                                                          <  2:941681/140‑1 (MQ=255)                                            gCCAACCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTa                                                                                         <  2:617423/140‑1 (MQ=255)                                                aCCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGAc                                                                                     >  2:972996/1‑140 (MQ=255)                                                     gATTTTCAGTCAGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGa                                                                                <  1:493382/140‑1 (MQ=255)                                                       ttttCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACt                                                                              >  2:619376/1‑140 (MQ=255)                                                                  gTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGGGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGAc                                                                   <  1:1010626/140‑1 (MQ=255)                                                                      tgctgcTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACaaaa                                                               <  1:536981/140‑1 (MQ=255)                                                                       gctgctGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAg                                                              <  1:345589/140‑1 (MQ=255)                                                                       gctgctGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAg                                                              <  1:972996/140‑1 (MQ=255)                                                                       gctgctGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAg                                                              <  1:1025646/140‑1 (MQ=255)                                                                       gctgctGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAg                                                              <  2:908754/140‑1 (MQ=255)                                                                            tgATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGta                                                         <  1:248334/140‑1 (MQ=255)                                                                            tgATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGta                                                         <  1:305846/140‑1 (MQ=255)                                                                            tgATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGta                                                         >  1:406439/1‑140 (MQ=255)                                                                            tgATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGta                                                         <  1:77726/140‑1 (MQ=255)                                                                                             atatTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCt                                        >  1:509323/1‑140 (MQ=255)                                                                                                ttGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCg                                     <  2:107842/140‑1 (MQ=255)                                                                                                  gctgcGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGc                                   >  1:495704/1‑140 (MQ=255)                                                                                                  gctgcGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGc                                   <  1:31062/140‑1 (MQ=255)                                                                                                   ctgcGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGcc                                  <  2:887094/140‑1 (MQ=255)                                                                                                   ctgcGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGcc                                  <  2:570660/140‑1 (MQ=255)                                                                                                      cGTTTACGGCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGAC‑AAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAAt                              >  1:750184/1‑140 (MQ=255)                                                                                                        tttACGGCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGAC‑AAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAAttt                            <  2:750184/140‑1 (MQ=255)                                                                                                             ggCGATTGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAAt                        <  2:38615/140‑1 (MQ=255)                                                                                                                  ttGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGcc                                  <  1:157760/125‑1 (MQ=255)                                                                                                                  ttGAAATGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGcc                                  >  2:157760/1‑125 (MQ=255)                                                                                                                        tGGCGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGtt             <  1:297922/140‑1 (MQ=255)                                                                                                                           cGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGt                   >  1:107656/1‑131 (MQ=255)                                                                                                                           cGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGt                   <  2:107656/131‑1 (MQ=255)                                                                                                                           cGGAAAACGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTgg          >  2:581357/1‑140 (MQ=255)                                                                                                                                 aCGGCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCGAgg    <  1:581357/140‑1 (MQ=255)                                                                                                                                   ggCGACATCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCGAGGGc  <  2:60977/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                     CGGCGACAAATACAAAACCAACCTTTCTCACTCCACCTGGAATGCCAACCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCGAGGGC  >  NC_000913/708510‑708779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑