Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,851,992	C→A	R43R (CGC→CGA)	hypC →	hydrogenase 3 maturation protein HypC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,851,992	0	C	A	100.0%	219.2 / NA	61	R43R (CGC→CGA)	hypC	hydrogenase 3 maturation protein HypC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (31/30);  total (31/30)

ACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATGGCGAGG  >  NC_000913/2851858‑2852128                                                                                                                                       |                                                                                                                                          acaGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTggg                                                                                                                                      <  2:12711/140‑1 (MQ=255)  caGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGc                                                                                                                                     >  2:587181/1‑140 (MQ=255)  caGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGc                                                                                                                                     >  1:410239/1‑140 (MQ=255)    gCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCa                                                                                                                                   <  1:764360/140‑1 (MQ=255)      gATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGt                                                                                                                                 <  1:717254/140‑1 (MQ=255)       aTGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTg                                                                                                                                <  2:789666/140‑1 (MQ=255)       aTGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTg                                                                                                                                >  1:794135/1‑140 (MQ=255)       aTGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTg                                                                                                                                <  2:410239/140‑1 (MQ=255)           gCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTa                                                                                                                            >  2:1017716/1‑140 (MQ=255)                    ttCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTacac                                                                                                                   >  1:91007/1‑140 (MQ=255)                    ttCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTacac                                                                                                                   >  1:256884/1‑140 (MQ=255)                    ttCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTacac                                                                                                                   >  1:689603/1‑140 (MQ=255)                     tCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACg                                                                                                                  <  1:662065/140‑1 (MQ=255)                      cccGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGt                                                                                                                 >  2:484107/1‑140 (MQ=255)                           ccAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCt                                                                                                           >  1:72604/1‑140 (MQ=255)                           ccAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCt                                                                                                           >  1:621669/1‑140 (MQ=255)                                 ccGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCa                                                                                                     <  2:238887/140‑1 (MQ=255)                                    cACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGa                                                                                                  >  1:242074/1‑140 (MQ=255)                                         ttGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGta                                                                                             <  1:587181/140‑1 (MQ=255)                                         ttGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGta                                                                                             >  1:144381/1‑140 (MQ=255)                                         ttGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGta                                                                                             >  1:377249/1‑140 (MQ=255)                                         ttGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGta                                                                                             >  1:368738/1‑140 (MQ=255)                                          tGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGtaa                                                                                            <  2:72604/140‑1 (MQ=255)                                            aCGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGtaatt                                                                                          >  2:39088/1‑140 (MQ=255)                                                   ccAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAg                                                                                   >  2:289900/1‑140 (MQ=255)                                                           aaGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCa                                                                           <  2:242074/140‑1 (MQ=255)                                                           aaGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCa                                                                           <  2:57325/140‑1 (MQ=255)                                                             gTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCAcg                                                                         <  1:484107/140‑1 (MQ=255)                                                                  cGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGaca                                                                    <  2:426351/140‑1 (MQ=255)                                                                     cTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACActc                                                                 >  2:42162/1‑140 (MQ=255)                                                                           cATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACg                                                           <  2:368738/140‑1 (MQ=255)                                                                           cATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACg                                                           <  1:1051296/140‑1 (MQ=255)                                                                            aTTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGc                                                          <  1:551212/140‑1 (MQ=255)                                                                                aGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTa                                                      <  2:689603/140‑1 (MQ=255)                                                                                aGCGCGATGTCGATTTAACGTTAGTCAGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCTCGCGACACTCTCGACGCCTTa                                                      >  2:310783/1‑140 (MQ=255)                                                                                 gcgcGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTAc                                                     <  2:377249/140‑1 (MQ=255)                                                                                  cgcgATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTa                                                      <  2:967126/138‑1 (MQ=255)                                                                                  cgcgATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTa                                                      >  1:967126/1‑138 (MQ=255)                                                                                  cgcgATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTACa                                                    >  1:214373/1‑140 (MQ=255)                                                                                  cgcgATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTACa                                                    >  2:406401/1‑140 (MQ=255)                                                                                       tGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTACAAAACa                                               >  1:263591/1‑140 (MQ=255)                                                                                       tGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTACAAAACa                                               >  1:588406/1‑140 (MQ=255)                                                                                         tCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTACAAAACATg                                             >  1:537270/1‑140 (MQ=255)                                                                                                 aCGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTACAAAACATGTTTGACGt                                     >  2:408003/1‑140 (MQ=255)                                                                                                 aCGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTAAAAAACATGTTTGACGt                                     >  1:223309/1‑140 (MQ=255)                                                                                                       gTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTTGGGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGc                                >  2:863510/1‑140 (MQ=255)                                                                                                       gTCGGCAGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGcc                               >  2:46807/1‑140 (MQ=255)                                                                                                               cTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCg                       <  1:731251/140‑1 (MQ=255)                                                                                                                  cGATGAAAACGTTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGgcg                    <  2:223309/140‑1 (MQ=255)                                                                                                                  cGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCGCGCGACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGgcg                    <  2:91007/140‑1 (MQ=255)                                                                                                                  cGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGgcg                    <  1:289900/140‑1 (MQ=255)                                                                                                                  cGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGgcg                    >  1:1056571/1‑140 (MQ=255)                                                                                                                  cGATGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGgcg                    <  2:144381/140‑1 (MQ=255)                                                                                                                    aTGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGgcgcg                  <  2:588406/140‑1 (MQ=255)                                                                                                                    aTGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGgcgcg                  <  2:605728/140‑1 (MQ=255)                                                                                                                    aTGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGgcgcg                  <  1:406401/140‑1 (MQ=255)                                                                                                                    aTGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGgcgcg                  <  2:256884/140‑1 (MQ=255)                                                                                                                    aTGAAAACGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGgcgcg                  <  2:1056571/140‑1 (MQ=255)                                                                                                                       aaaaCGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCGCtg               <  1:39088/140‑1 (MQ=255)                                                                                                                       aaaaCGGTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCGCtg               <  1:1017716/140‑1 (MQ=255)                                                                                                                             gTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTAt         >  1:10830/1‑140 (MQ=255)                                                                                                                             gTCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTAt         >  2:617369/1‑140 (MQ=255)                                                                                                                              tCAGCCGCGAGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATg        >  1:955341/1‑140 (MQ=255)                                                                                                                                    gcgagTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATGGCGAgg  <  2:794135/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                          ACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTGGGTACTGGTACACGTT‑GGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATGGCGAGG  >  NC_000913/2851858‑2852128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑