BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,981,635	A→C	V183G (GTT→GGT)	araE ←	arabinose:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,981,635	0	A	C	98.7%	291.7 / ‑4.2	79	V183G (GTT→GGT)	araE	arabinose:H(+) symporter
Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (36/42); total (37/42)
Fisher's exact test for biased strand distribution p-value = 4.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

GCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATGCCGAGTGTGACCATCAACTGGTACATACTGATCATCTTACCGCGAACGTTTTCACTTGCCATTTCAGA  >  NC_000913/2981498‑2981771

gcgcTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAccc                                                                                                                                        >  1:971367/1‑140 (MQ=255)

  gcTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACcccc                                                                                                                                      >  1:747815/1‑140 (MQ=255)

              gTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTgcgcg                                                                                                                          >  2:264144/1‑140 (MQ=255)

              gTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTgcgcg                                                                                                                          <  2:402586/140‑1 (MQ=255)

                    cgcAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGtt                                                                                                                    <  1:169648/140‑1 (MQ=255)

                    cgcAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGtt                                                                                                                    <  1:975191/140‑1 (MQ=255)

                    cgcAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGtt                                                                                                                    <  1:667834/140‑1 (MQ=255)

                     gcagcaTACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTa                                                                                                                   >  1:147886/1‑140 (MQ=255)

                      cagcaTACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTAc                                                                                                                  <  1:667391/140‑1 (MQ=255)

                      cagcaTACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTAc                                                                                                                  <  2:663338/140‑1 (MQ=255)

                         caTACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGTTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGc                                                                                                               <  2:767056/140‑1 (MQ=255)

                         caTACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGc                                                                                                               >  2:528257/1‑140 (MQ=255)

                          aTACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCt                                                                                                              <  2:836976/140‑1 (MQ=255)

                              gCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATaa                                                                                                          <  2:751226/140‑1 (MQ=255)

                                  tACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGa                                                                                                      >  2:639795/1‑140 (MQ=255)

                                   aCTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGaa                                                                                                     >  2:460924/1‑140 (MQ=255)

                                   aCTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGaa                                                                                                     >  2:1031803/1‑140 (MQ=255)

                                    cttcttCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAAc                                                                                                    <  2:77544/140‑1 (MQ=255)

                                     ttcttcCGCCTCAATATGCCGCGCCTGTTCCGCCAGCCAGCGCGGGTTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTTGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGTACg                                                                                                   <  2:466541/140‑1 (MQ=255)

                                     ttcttcCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACg                                                                                                   >  2:263423/1‑140 (MQ=255)

                                       cttcCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCt                                                                                                 >  1:529239/1‑140 (MQ=255)

                                       cttcCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCt                                                                                                 >  2:865110/1‑140 (MQ=255)

                                        ttcCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTg                                                                                                >  2:363468/1‑140 (MQ=255)

                                         tcCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGt                                                                                               >  2:1019271/1‑140 (MQ=255)

                                            gCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATc                                                                                            >  1:588484/1‑140 (MQ=255)

                                             ccTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCg                                                                                           >  2:456155/1‑140 (MQ=255)

                                               tCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAAc                                                                                                    <  1:992287/129‑1 (MQ=255)

                                               tCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAAc                                                                                                    >  2:992287/1‑129 (MQ=255)

                                                 aaTATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATa                                                                                       >  1:104492/1‑140 (MQ=255)

                                                   tatGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATaaa                                                                                     <  1:692302/140‑1 (MQ=255)

                                                        cGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGc                                                                                >  1:140822/1‑140 (MQ=255)

                                                           cccTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCgg                                                                                          <  1:430629/127‑1 (MQ=255)

                                                           cccTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCgg                                                                                          >  2:430629/1‑127 (MQ=255)

                                                           cccTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAg                                                                             >  1:778844/1‑140 (MQ=255)

                                                           cccTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAg                                                                             >  1:496199/1‑140 (MQ=255)

                                                           cccTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAg                                                                             >  2:582005/1‑140 (MQ=255)

                                                           cccTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAg                                                                             >  1:662372/1‑140 (MQ=255)

                                                             cTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCa                                                                           >  2:681084/1‑140 (MQ=255)

                                                             cTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCa                                                                           >  2:535444/1‑140 (MQ=255)

                                                               tttCCGCCAGCCAGCGCTGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACg                                                                         >  1:45946/1‑140 (MQ=255)

                                                               tttCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACg                                                                         >  1:817502/1‑140 (MQ=255)

                                                                  ccGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATg                                                                      <  1:639795/140‑1 (MQ=255)

                                                                  ccGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATg                                                                      <  1:865110/140‑1 (MQ=255)

                                                                  ccGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATg                                                                      <  1:80382/140‑1 (MQ=255)

                                                                     ccagccagCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATGCCg                                                                   <  1:1031803/140‑1 (MQ=255)

                                                                         ccagcGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATGCCGAgtg                                                               <  1:264144/140‑1 (MQ=255)

                                                                         ccagcGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATGCCGAgtg                                                               >  2:644130/1‑140 (MQ=255)

                                                                         ccagcGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATGCCGAgtg                                                               <  1:644130/140‑1 (MQ=255)

                                                                          cagcGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATGCCGAgtgt                                                              <  2:588695/140‑1 (MQ=255)

                                                                               cgGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATGCCGAGTGTGACCa                                                         <  1:456155/140‑1 (MQ=255)

                                                                                  gCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATGCCGAGTGTGACCATCa                                                      >  2:152546/1‑140 (MQ=255)

                                                                                  gCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATGCCGAGTGTGACCATCa                                                      >  2:814290/1‑140 (MQ=255)

                                                                                  gCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATGCCGAGTGTGACCATCa                                                      >  1:696802/1‑140 (MQ=255)

                                                                                   gtATTTGGCAGGAAGACGAACAGAATAATCAGCAGATCTGCTTGTAACACAAGAACCCCCAGCAAGGCGCGTCGGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCGGCACGATGCCGAGTGTGACCATCat                                                     <  2:696802/139‑2 (MQ=255)

                                                                                   cTATTTGGCAGGAGGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATGCCGAGTGTGACCATCaa                                                     <  2:18425/140‑1 (MQ=255)

                                                                                   cTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATGCCGAGTGTGACCATCaa                                                     <  1:363468/140‑1 (MQ=255)

                                                                                   cTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATGCCGAGTGTGACCATCaa                                                     <  2:967350/140‑1 (MQ=255)

                                                                                   cTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATGCCGAGTGTGACCATCaa                                                     <  1:263423/140‑1 (MQ=255)

                                                                                   cTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATGCCGAGTGTGACCATCaa                                                     <  2:817502/140‑1 (MQ=255)

                                                                                   cTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATGCCGAGTGTGACCATCaa                                                     <  1:528257/140‑1 (MQ=255)

                                                                                      tttGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATGCCGAGTGTGACCATCAACTg                                                  <  2:485488/140‑1 (MQ=255)

                                                                                        tGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATGCCGAGTGTGACCATCAACTGGt                                                <  2:739492/140‑1 (MQ=255)

                                                                                        tGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATGCCGAGTGTGACCATCAACTGGt                                                <  2:685546/140‑1 (MQ=255)

                                                                                        tGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATGCCGAGTGTGACCATCAACTGGt                                                <  2:529239/140‑1 (MQ=255)

                                                                                        tGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATGCCGAGTGTGACCATCAACTGGt                                                <  2:21924/140‑1 (MQ=255)

                                                                                        tGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATGCCGAGTGTGACCATCAACTGGt                                                <  2:394058/140‑1 (MQ=255)

                                                                                        tGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATGCCGAGTGTGACCATCAACTGGt                                                <  2:147886/140‑1 (MQ=255)

                                                                                          gCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATGCCGAGTGTGACCATCAACTGGTAc                                              <  1:460924/140‑1 (MQ=255)

                                                                                             ggAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATGCCGAGTGTGACCATCAACTGGTACATa                                           <  1:379415/140‑1 (MQ=255)

                                                                                             ggAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATGCCGAGTGTGACCATCAACTGGTACATa                                           <  1:1019271/140‑1 (MQ=255)

                                                                                              gAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATGCCGAGTGTGACCATCAACTGGTACATAc                                          >  1:89087/1‑140 (MQ=255)

                                                                                                         gAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCGAGCACGATGCCGAGTGTGACCATCAACTGGTACATACTGATCATCTTa                               >  2:801699/1‑140 (MQ=255)

                                                                                                         gAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATGCCGAGTGTGACCATCAACTGGTACATACTGATCATCTTa                               >  2:615178/1‑140 (MQ=255)

                                                                                                         gAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATGCCGAGTGTGACCATCAACTGGTACATACTGATCATCTTa                               >  1:97482/1‑140 (MQ=255)

                                                                                                         gAATAATCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATGCCGA‑‑GTGACCATCAACTGGGACATACTGATCATCTTAcc                             >  2:236368/1‑140 (MQ=255)

                                                                                                          aataatCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATGCCGAGTGTGACCATCAACTGGTACATACTGATCATCTTAc                              >  1:228223/1‑140 (MQ=255)

                                                                                                               tCAGCAGAACTGCTGGTAAAGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATGCCGAGTGTGACCATCAACTGGTACATACTGATCATCTTACCGCGa                         >  1:813080/1‑140 (MQ=255)

                                                                                                                                  aGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATGCCGAGTGTGACCATCAACTGGTACATACTGATCATCTTACCGCGAACGTTTTCACTTGCCAttt      <  1:615178/140‑1 (MQ=255)

                                                                                                                                  aGCAAGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATGCCGAGTGTGACCATCAACTGGTACATACTGATCATCTTACCGCGAACGTTTTCACTTGCCAttt      <  2:496199/140‑1 (MQ=255)

                                                                                                                                     aaGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATGCCGAGTGTGACCATCAACTGGTACATACTGATCATCTTACCGCGAACGTTTTCACTTGCCATTTCAg   <  1:343659/140‑1 (MQ=255)

                                                                                                                                     aaGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATGCCGAGTGTGACCATCAACTGGTACATACTGATCATCTTACCGCGAACGTTTTCACTTGCCATTTCAg   <  2:813080/140‑1 (MQ=255)

                                                                                                                                      aGACCCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATGCCGAGTGTGACCATCAACTGGTACATACTGATCATCTTACCGCGAACGTTTTCACTTGCCATTTCAGa  <  2:104492/140‑1 (MQ=255)

GCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAAAACGCCAGCACGATGCCGAGTGTGACCATCAACTGGTACATACTGATCATCTTACCGCGAACGTTTTCACTTGCCATTTCAGA  >  NC_000913/2981498‑2981771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`