Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,001,918	0	A	C	32.5%	151.0 / 38.4	80	intergenic (‑129/+192)	fliA/fliC	RNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein
Reads supporting (aligned to +/- strand):  ref base A (51/3);  new base C (0/26);  total (51/29)
Fisher's exact test for biased strand distribution p-value = 4.75e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCA  >  NC_000913/2001781‑2002055                                                                                                                                           |                                                                                                                                           tGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAAcc                                                                                                                                          >  1:1201351/1‑140 (MQ=255)   aaTTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAAcc‑cc                                                                                                                                       >  1:462170/1‑140 (MQ=255)   aaTTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAAcc‑cc                                                                                                                                       >  1:587139/1‑140 (MQ=255)   aaTTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAAcc‑cc                                                                                                                                       >  1:1758815/1‑140 (MQ=255)    aTTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑ccg                                                                                                                                      >  1:2061832/1‑140 (MQ=255)    aTTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑ccg                                                                                                                                      >  2:2395021/1‑140 (MQ=255)      tCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑ccgcc                                                                                                                                    >  2:1087817/1‑140 (MQ=255)                  gCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGaa                                                                                                                        >  1:1469979/1‑140 (MQ=255)                   cccTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAg                                                                                                                       >  1:298979/1‑140 (MQ=255)                      tGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGGAc                                                                                                                    >  2:1888525/1‑140 (MQ=255)                      tGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCAc                                                                                                                    >  1:1778347/1‑140 (MQ=255)                      tGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCAc                                                                                                                    >  1:228463/1‑140 (MQ=255)                        ttttATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGTTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑AAAGGTAAATTCCAGGCAAAAAAAACCC‑CCGCCGGTGGCGGGGAAGCACGt                                                                                                                  <  2:1201351/138‑1 (MQ=255)                             tatGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTg                                                                                                             >  2:909099/1‑140 (MQ=255)                                  gTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACaaa                                                                                                        >  1:2394461/1‑140 (MQ=255)                                    tATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATGGGACGATTGGGGGGTGAAAGGGGGGGTTTTTGGGGGGTT‑ACAGGTAAATTCCAGGCAAAAAAAACCC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAAtt                                                                                                      <  2:1243006/140‑1 (MQ=255)                                    tATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGGGGGTAAAAGGGGGGGTTTTTGGGGGGTT‑ACAGGAAAATTCCAGGAAAAAAAAACCC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAAtt                                                                                                      <  2:1557862/140‑1 (MQ=255)                                     aTCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTg                                                                                                     >  2:191356/1‑140 (MQ=255)                                     aTCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTg                                                                                                     >  1:1243006/1‑140 (MQ=255)                                     aTCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTg                                                                                                     >  1:540318/1‑140 (MQ=255)                                       gggCATGTTTATCGGTTTCGGCGGGTTTTTTAACGGGCCGAGTAGTGGG‑GAAAAGGGGGGGTTTTTGGGGGTTGAAAGTGAAATGCCA‑GCAAAAAAAAACCGCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTgcg                                                                                                   <  2:963828/139‑1 (MQ=255)                                       cGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTgcg                                                                                                   >  1:1801764/1‑140 (MQ=255)                                       cGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTgcg                                                                                                   >  1:1137006/1‑140 (MQ=255)                                             gATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTAt                                                                                             >  1:1108248/1‑140 (MQ=255)                                              aTTATCCGTTTCTGCGGGGTTTTTAATCGGACGTTTGGGGGGGGAAATGAGGGGTTTTTGGGGGGTT‑ACAGGTAAATCCCAGGCAAAAAAAACCC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATg                                                                                            <  2:1801764/140‑1 (MQ=255)                                                  tCCGTTTCTGCAGTTTTTTTAATCGGACGATTAGGGGGTGAAATGGGGGGTTATTGGGGGGTT‑ACAGGAAAATTCCAGGAAAAAAAAACCC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGc                                                                                        <  2:1314930/140‑1 (MQ=255)                                                      tttCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGa                                                                                    >  1:939967/1‑140 (MQ=255)                                                       ttCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGAt                                                                                   >  1:1194598/1‑140 (MQ=255)                                                       ttCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGAt                                                                                   >  1:399740/1‑140 (MQ=255)                                                        tCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATg                                                                                  >  2:206124/1‑140 (MQ=255)                                                         cTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgc                                                                                 >  1:2333804/1‑140 (MQ=255)                                                         cTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgc                                                                                 >  2:2583158/1‑140 (MQ=255)                                                          tGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgcg                                                                                >  1:193246/1‑140 (MQ=255)                                                            cAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgcggc                                                                              >  1:1815228/1‑140 (MQ=255)                                                            cAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgcggc                                                                              >  2:1161093/1‑140 (MQ=255)                                                              gggTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGt                                                                            >  1:506133/1‑140 (MQ=255)                                                               gttttttAATCGGAGGTTTAGTGGGTAAAAGGGGGGTTTATTTGGGGGTA‑ACAGGTAATTTCCGGGAAAAAAAAACCC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTa                                                                           <  2:540318/138‑1 (MQ=255)                                                               gttttttAATCGGAAGATTAGTGGGTAAAATGGGGGGTTTTTTGGGGGTT‑ACAGTTAAATTCCAGGAAAAAAAACCCC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTa                                                                           <  2:1778347/138‑1 (MQ=255)                                                                   tttAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACg                                                                       >  1:380358/1‑140 (MQ=255)                                                                   tttAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACg                                                                       >  1:579293/1‑140 (MQ=255)                                                                   tttAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACg                                                                       >  1:82637/1‑140 (MQ=255)                                                                          ggaggaTTGGTGGGTGAAAGGGGGGTTTTTTGGGGGGTT‑ACAGGTAAATTCCGGGCAGAAAAAACCC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATc                                                                <  1:1888525/140‑1 (MQ=38)                                                                          ggACGATTGGGGGGTAAAATGGGGGGTTTTTGGGGGGTT‑AAAGGAAAATCCCAGGAAAAAAAAACCC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATc                                                                <  2:1985580/140‑1 (MQ=37)                                                                          ggACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAACCC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATc                                                                <  1:2096686/140‑1 (MQ=255)                                                                          ggACGATTAGTGGGTAAAATGGGGGTTTATTGGGGGTTT‑ACAGGAAAATCCCAGGAAAAAAAAACCC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATc                                                                <  2:2394461/140‑1 (MQ=38)                                                                          ggACGATTAGGGGGTGAAATGAGGGGTTATTGGGGGGTT‑ACAGGTAAATCCCAGGCAAAAAAAACCC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATc                                                                <  2:1815228/140‑1 (MQ=39)                                                                           gACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATcc                                                               >  1:968793/1‑140 (MQ=255)                                                                           gACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATcc                                                               >  1:1923361/1‑140 (MQ=255)                                                                           gACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATcc                                                               >  1:2382820/1‑140 (MQ=255)                                                                            aCGATTAGTGGGTAAAATGAGGGTTTATTGGGGGGTT‑AAAGGTAAATTCCAGGCAGAAAAAACCC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCg                                                              <  1:1317900/140‑1 (MQ=39)                                                                            aCGATTAGGGGGTGAAATGGGGGGTTTTTGGGGGGTT‑AAAGGAAAATCCCAGGAAAAAAAAACCC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCg                                                              <  2:1108248/140‑1 (MQ=37)                                                                            aCGATTAGGGGGTGAAATGGGGGGTTATTGGGGGTTT‑ACAGTAAAATCCCAGGAAAAAAAAACCC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCg                                                              <  2:298979/140‑1 (MQ=37)                                                                              gATTAGGGGGTAAAATGGGGGGTTTTTCGGGGGTT‑AAAGTTAATTCCAAGGAAAAAAAAACCC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                            <  2:2582831/140‑1 (MQ=25)                                                                                      ggTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa                                                    >  1:1213974/1‑140 (MQ=255)                                                                                      ggTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa                                                    >  1:2582831/1‑140 (MQ=255)                                                                                      ggTAAAATGAGGGTTTATTTGGGGGTC‑ACAGGTAAATTCCAGCAAGAAAAAACCC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa                                                    <  2:1428654/140‑1 (MQ=38)                                                                                            aggAGGGGTTTTTGGGGGGTT‑ACAGGAAAATCCCAGGCAAAAAAAACCC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGc                                              <  2:968793/138‑1 (MQ=37)                                                                                             tGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCg                                                              >  2:2241028/1‑123 (MQ=255)                                                                                             tGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCg                                                              <  1:2241028/123‑1 (MQ=255)                                                                                             ggAGGGTTTTTTTGGGGTTT‑ACAGTAAAATTCCAGGAAAAAAAACCCC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCa                                             <  2:193246/139‑1 (MQ=37)                                                                                                  ggTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTc                                        >  1:1518227/1‑140 (MQ=255)                                                                                                     tATTGGGGGGTT‑ACAGGTAAATCCCAGGAAAAAAAAACCC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAAt                                     <  2:380358/140‑1 (MQ=37)                                                                                                           ggggTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTg                               >  2:1022097/1‑140 (MQ=255)                                                                                                              gTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCaa                            >  2:621989/1‑140 (MQ=255)                                                                                                                  aCAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTc                         >  2:1244946/1‑140 (MQ=255)                                                                                                                  aCAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTc                         >  2:2591546/1‑140 (MQ=255)                                                                                                                  aCAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTc                         >  1:532839/1‑140 (MQ=255)                                                                                                                   cAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCa                        <  1:191356/140‑1 (MQ=255)                                                                                                                   agggTAAATCCCAGGAAGAAAAAACCC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCa                        <  2:462170/138‑1 (MQ=255)                                                                                                                   aaGGAAAATCCCAGGAAAAAAAAACCC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCa                        <  2:1518227/139‑1 (MQ=37)                                                                                                                      gTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACt                     >  2:631206/1‑140 (MQ=255)                                                                                                                        aaaTTCCAGGCAGAAAAAACCC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTg                   <  1:2591546/140‑1 (MQ=255)                                                                                                                        aaaTTCCAGGAAAAAAAAACCC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTg                   <  1:909099/140‑1 (MQ=255)                                                                                                                          atcccAGGAAAAAAAAACCC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTa                 <  2:2382820/137‑1 (MQ=255)                                                                                                                             ccAGGCAGAAAAAACCC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGc              <  2:399740/140‑1 (MQ=255)                                                                                                                             ccAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGc              <  2:1137006/140‑1 (MQ=255)                                                                                                                             ccAGGAAAAAAAAACCC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGc              <  2:1923361/140‑1 (MQ=255)                                                                                                                               aGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCt            >  2:996373/1‑140 (MQ=255)                                                                                                                                ggCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGt                  >  1:1894917/1‑133 (MQ=255)                                                                                                                                ggCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGt                  <  2:1894917/133‑1 (MQ=255)                                                                                                                                ggCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTg           >  2:2124775/1‑140 (MQ=255)                                                                                                                                ggCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTg           >  1:2534043/1‑140 (MQ=255)                                                                                                                                ggCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTg           >  2:1409891/1‑140 (MQ=255)                                                                                                                                  cAGAAAAAACCC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGAt         <  1:2583158/140‑1 (MQ=255)                                                                                                                                     aaaaaaaCC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAag      >  1:1547220/1‑140 (MQ=255)                                                                                                                                         aaaCC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgca  <  1:631206/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                           TGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCA  >  NC_000913/2001781‑2002055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑